Click here to switch to the map view.

The map label for this gene is gloB [H]

Identifier: 16273189

GI number: 16273189

Start: 1350114

End: 1350830

Strand: Direct

Name: gloB [H]

Synonym: HI1274

Alternate gene names: 16273189

Gene position: 1350114-1350830 (Clockwise)

Preceding gene: 16273188

Following gene: 30995435

Centisome position: 73.77

GC content: 40.17

Gene sequence:

>717_bases
ATGTTATTTGCTTTACCTGCGCTGAATGATAACTACATTTGGCTTTATCAACGAGAAAATCTGCCGCTTATTATTGTGGA
TTTGCCTGAAACGGACAAGCTGTTTGCATGGCTAGAGAAGCAAAACGCAACGATTGAAGCGGTGTTGCTTACTCACGAAC
ATGATGATCATACACAAGGTGTGTCGGCATTTAAAAAACGTTATCCAACAGTGCCGATTTATGGGCCGCAAGAATGTGAG
AAAAAAGGTGCGACTCAAATTGTGAACGAAGGGAAGATCCTTACCGCAAATTATCAAATTGATGTCATTCCGACGGGGGG
ACACACTAAACAGCACGTTAGTTTTTTAGTGGATAATCATTTATTTTGTGGTGATGCGTTATTTTCTGCTGGTTGCGGAC
GTGTTTTCACAGGTAATTATGCGCTGATGTTTGAAGGTTTACAGCGTTTGAATACATTGCCTGACGAGACGATTGTTTGC
CCAGCTCATGAATATACCTTGGGGAATTTAGCATTTGCGGAAACTGTGCTGGTGGATAAAAGTGCGGTAGAAAAAAGTGC
GGTAGAAAAACAACGTATTTTTGTTGAAACACAACGGGCTGAAAATAAACCGAGTTTGCCAACAACCTTAAAACTAGAGC
GAGAAATTAATCCGTTTTTACAAGCAAAAACACTTGAAGAATTTACCGCACTTCGTAAAGCCAAAGATATTTTCTAA

Upstream 100 bases:

>100_bases
CGGACAGAAAAAATGGGCGTTAATTTGGTGGAATGTGGATCGCATCTAATGTTTCAAGTGGAATCAATTTATTTTTCTAT
CACTAATTAAAAGGACGATT

Downstream 100 bases:

>100_bases
GTTATCTTTTTATTGGGGGGGAGCACAAAGTGCGGTGATTTTTTAAATTAGCAGATTATTTCTTTCTCGCTAACATGGTG
GCAAATTTCATTTTAATGCG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MLFALPALNDNYIWLYQRENLPLIIVDLPETDKLFAWLEKQNATIEAVLLTHEHDDHTQGVSAFKKRYPTVPIYGPQECE
KKGATQIVNEGKILTANYQIDVIPTGGHTKQHVSFLVDNHLFCGDALFSAGCGRVFTGNYALMFEGLQRLNTLPDETIVC
PAHEYTLGNLAFAETVLVDKSAVEKSAVEKQRIFVETQRAENKPSLPTTLKLEREINPFLQAKTLEEFTALRKAKDIF

Sequences:

>Translated_238_residues
MLFALPALNDNYIWLYQRENLPLIIVDLPETDKLFAWLEKQNATIEAVLLTHEHDDHTQGVSAFKKRYPTVPIYGPQECE
KKGATQIVNEGKILTANYQIDVIPTGGHTKQHVSFLVDNHLFCGDALFSAGCGRVFTGNYALMFEGLQRLNTLPDETIVC
PAHEYTLGNLAFAETVLVDKSAVEKSAVEKQRIFVETQRAENKPSLPTTLKLEREINPFLQAKTLEEFTALRKAKDIF
>Mature_238_residues
MLFALPALNDNYIWLYQRENLPLIIVDLPETDKLFAWLEKQNATIEAVLLTHEHDDHTQGVSAFKKRYPTVPIYGPQECE
KKGATQIVNEGKILTANYQIDVIPTGGHTKQHVSFLVDNHLFCGDALFSAGCGRVFTGNYALMFEGLQRLNTLPDETIVC
PAHEYTLGNLAFAETVLVDKSAVEKSAVEKQRIFVETQRAENKPSLPTTLKLEREINPFLQAKTLEEFTALRKAKDIF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI21703352, Length=239, Percent_Identity=34.3096234309623, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI116642887, Length=233, Percent_Identity=34.3347639484979, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI14150041, Length=244, Percent_Identity=29.5081967213115, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI94538320, Length=256, Percent_Identity=30.078125, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI94538322, Length=256, Percent_Identity=30.078125, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI46361987, Length=180, Percent_Identity=32.2222222222222, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1786406, Length=254, Percent_Identity=37.7952755905512, Blast_Score=170, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17536925, Length=232, Percent_Identity=31.8965517241379, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320478, Length=231, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356335, Length=258, Percent_Identity=29.8449612403101, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24667711, Length=258, Percent_Identity=29.8449612403101, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24667703, Length=258, Percent_Identity=29.8449612403101, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 26850; Mature: 26850

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFALPALNDNYIWLYQRENLPLIIVDLPETDKLFAWLEKQNATIEAVLLTHEHDDHTQG
CEEEECCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHCCCCEEEEEEEEECCCCHHHH
VSAFKKRYPTVPIYGPQECEKKGATQIVNEGKILTANYQIDVIPTGGHTKQHVSFLVDNH
HHHHHHHCCCCCCCCCHHHHHCCCHHHHCCCEEEEEEEEEEEEECCCCCHHHHHHHHCCC
LFCGDALFSAGCGRVFTGNYALMFEGLQRLNTLPDETIVCPAHEYTLGNLAFAETVLVDK
EEECHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCH
SAVEKSAVEKQRIFVETQRAENKPSLPTTLKLEREINPFLQAKTLEEFTALRKAKDIF
HHHHHHHHHHHHHEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLFALPALNDNYIWLYQRENLPLIIVDLPETDKLFAWLEKQNATIEAVLLTHEHDDHTQG
CEEEECCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHCCCCEEEEEEEEECCCCHHHH
VSAFKKRYPTVPIYGPQECEKKGATQIVNEGKILTANYQIDVIPTGGHTKQHVSFLVDNH
HHHHHHHCCCCCCCCCHHHHHCCCHHHHCCCEEEEEEEEEEEEECCCCCHHHHHHHHCCC
LFCGDALFSAGCGRVFTGNYALMFEGLQRLNTLPDETIVCPAHEYTLGNLAFAETVLVDK
EEECHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCH
SAVEKSAVEKQRIFVETQRAENKPSLPTTLKLEREINPFLQAKTLEEFTALRKAKDIF
HHHHHHHHHHHHHEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA