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The map label for this gene is murD [H]

Identifier: 16273062

GI number: 16273062

Start: 1203734

End: 1205047

Strand: Direct

Name: murD [H]

Synonym: HI1136

Alternate gene names: 16273062

Gene position: 1203734-1205047 (Clockwise)

Preceding gene: 16273061

Following gene: 16273063

Centisome position: 65.77

GC content: 38.66

Gene sequence:

>1314_bases
ATGAACGCCTATCAAAACAAAAATATTACTATCATCGGGCTTGGCAAAACAGGTCTTTCTTGTGTGGATTATCTCTTATC
CCAACAGGCTAATATTCGTGTGATTGATACTCGAAAAAATCCTACTGGTATTGATAAACTTCCTCAAAATATCCCTCTTC
ATACTGGTAGTTTAAATCAGGAATGGTTACTTGAAAGCGATATGATTGTTATTAGCCCAGGGCTTGCGGTAAAAACACCA
GAAATTCAAACCGCACTTAAAGCGGGAGTGGAAGTAATCGGCGATATTGAATTATTCTGCCGCGCAGCGACAAAGCCAAT
TGTGGGGATTACAGGTTCAAATGGTAAAAGTACCGTAACTACTTTAGTTTATGAAATGGCGAAAGCTGCTGGTGTGAAAG
TTGGTATGGGCGGAAATATTGGGATTCCCGCTTTGTCATTGTTGAATGAAGATTGTGAACTTTATGTACTAGAGCTTTCT
AGTTTTCAGCTTGAGACAACTTATAGCTTAAAAGCTGCGGCAGCGACTGTCTTGAACGTGACTGAAGATCATATGGATCG
CTATATGGATTTAGAAGATTATCGCCAAGCAAAATTACGCATTTATCATAATGCTAAAGTAGGTGTGTTGAACAATGAAG
ATAGGCTGACTTTTGGGGAAAACGAAAATCAAGCGAAACATACCGTTTCTTTTGCGGAAAATAGTGCGGATTATTGGCTA
AAAACTGAAAATGGCAAGCAATATTTAATGGTAAAAGATGAAGTGATTTTACCTTGTGAAGAAGCTACATTGGTTGGTCG
CCATAATTATATGAACATTTTGGCAGCAACAGCATTGGCACAAGCTATAGGTATTAATTTAGATTCAATTCGTACCGCAC
TTCGTCATTTCAAAGGGTTAGATCATCGTTTTCAATTAGTGCATCAAGCTAATGGCATTCGTTGGATTAATGACTCTAAA
GCAACAAATGTGGGGAGTACAGTTGCTGCATTGGCTGGGCTTTATATTGAGGGTAAATTGCATTTGTTGCTAGGCGGAGA
CGGAAAAGGGGCTGATTTTTCAGAATTAGCTGAATTAATTAATCAACCACACATTATTTGTTATTGTTTTGGTCGAGATG
GTGCGCTGCTTGCAAAATTTTCATCGCAAAGTTATTTGTTCGATACAATGGAACAAGCGATAGAATTTTTACGCCCAACA
TTGCAAAGCGGAGATATGGTATTATTGTCGCCTGCTTGTGCAAGTCTCGATCAGTTTGCTTCTTTTGAAAAGCGCGGCGA
AGAATTTACGCATTTAGCTCAATGTTTAACCTAA

Upstream 100 bases:

>100_bases
TTCACTTTTGAAAGAGCGACCTTTTTTACGTTAGTAAAGGAAATTTATATTTCCATCAATGATATAATTTTAAAATTTAT
AGCAATTAAGAAGAAAGAAA

Downstream 100 bases:

>100_bases
TTTAGTAACTCACGAAATAAGATGGAATTTTTACAAAATATCAAAAAAAATTACGATGAATGGACACGCATCACACCTCA
AGGTTTGTTGTATGATCGCG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MNAYQNKNITIIGLGKTGLSCVDYLLSQQANIRVIDTRKNPTGIDKLPQNIPLHTGSLNQEWLLESDMIVISPGLAVKTP
EIQTALKAGVEVIGDIELFCRAATKPIVGITGSNGKSTVTTLVYEMAKAAGVKVGMGGNIGIPALSLLNEDCELYVLELS
SFQLETTYSLKAAAATVLNVTEDHMDRYMDLEDYRQAKLRIYHNAKVGVLNNEDRLTFGENENQAKHTVSFAENSADYWL
KTENGKQYLMVKDEVILPCEEATLVGRHNYMNILAATALAQAIGINLDSIRTALRHFKGLDHRFQLVHQANGIRWINDSK
ATNVGSTVAALAGLYIEGKLHLLLGGDGKGADFSELAELINQPHIICYCFGRDGALLAKFSSQSYLFDTMEQAIEFLRPT
LQSGDMVLLSPACASLDQFASFEKRGEEFTHLAQCLT

Sequences:

>Translated_437_residues
MNAYQNKNITIIGLGKTGLSCVDYLLSQQANIRVIDTRKNPTGIDKLPQNIPLHTGSLNQEWLLESDMIVISPGLAVKTP
EIQTALKAGVEVIGDIELFCRAATKPIVGITGSNGKSTVTTLVYEMAKAAGVKVGMGGNIGIPALSLLNEDCELYVLELS
SFQLETTYSLKAAAATVLNVTEDHMDRYMDLEDYRQAKLRIYHNAKVGVLNNEDRLTFGENENQAKHTVSFAENSADYWL
KTENGKQYLMVKDEVILPCEEATLVGRHNYMNILAATALAQAIGINLDSIRTALRHFKGLDHRFQLVHQANGIRWINDSK
ATNVGSTVAALAGLYIEGKLHLLLGGDGKGADFSELAELINQPHIICYCFGRDGALLAKFSSQSYLFDTMEQAIEFLRPT
LQSGDMVLLSPACASLDQFASFEKRGEEFTHLAQCLT
>Mature_437_residues
MNAYQNKNITIIGLGKTGLSCVDYLLSQQANIRVIDTRKNPTGIDKLPQNIPLHTGSLNQEWLLESDMIVISPGLAVKTP
EIQTALKAGVEVIGDIELFCRAATKPIVGITGSNGKSTVTTLVYEMAKAAGVKVGMGGNIGIPALSLLNEDCELYVLELS
SFQLETTYSLKAAAATVLNVTEDHMDRYMDLEDYRQAKLRIYHNAKVGVLNNEDRLTFGENENQAKHTVSFAENSADYWL
KTENGKQYLMVKDEVILPCEEATLVGRHNYMNILAATALAQAIGINLDSIRTALRHFKGLDHRFQLVHQANGIRWINDSK
ATNVGSTVAALAGLYIEGKLHLLLGGDGKGADFSELAELINQPHIICYCFGRDGALLAKFSSQSYLFDTMEQAIEFLRPT
LQSGDMVLLSPACASLDQFASFEKRGEEFTHLAQCLT

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=441, Percent_Identity=60.9977324263039, Blast_Score=506, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47907; Mature: 47907

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAYQNKNITIIGLGKTGLSCVDYLLSQQANIRVIDTRKNPTGIDKLPQNIPLHTGSLNQ
CCCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCCCC
EWLLESDMIVISPGLAVKTPEIQTALKAGVEVIGDIELFCRAATKPIVGITGSNGKSTVT
CCEEECCEEEECCCCEEECCHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCHHHH
TLVYEMAKAAGVKVGMGGNIGIPALSLLNEDCELYVLELSSFQLETTYSLKAAAATVLNV
HHHHHHHHHCCEEEECCCCCCCCHHHHCCCCCEEEEEEECCEEEEEEEEHHHHHHHHHHC
TEDHMDRYMDLEDYRQAKLRIYHNAKVGVLNNEDRLTFGENENQAKHTVSFAENSADYWL
CHHHHHHHCCHHHHHCEEEEEEECCEEEEECCCCCEEECCCCCCHHHEEEECCCCCCEEE
KTENGKQYLMVKDEVILPCEEATLVGRHNYMNILAATALAQAIGINLDSIRTALRHFKGL
EECCCCEEEEEECCEEECCCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
DHRFQLVHQANGIRWINDSKATNVGSTVAALAGLYIEGKLHLLLGGDGKGADFSELAELI
CHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCEEEECEEEEEECCCCCCCCHHHHHHHH
NQPHIICYCFGRDGALLAKFSSQSYLFDTMEQAIEFLRPTLQSGDMVLLSPACASLDQFA
CCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHH
SFEKRGEEFTHLAQCLT
HHHHHCHHHHHHHHHCC
>Mature Secondary Structure
MNAYQNKNITIIGLGKTGLSCVDYLLSQQANIRVIDTRKNPTGIDKLPQNIPLHTGSLNQ
CCCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCCCC
EWLLESDMIVISPGLAVKTPEIQTALKAGVEVIGDIELFCRAATKPIVGITGSNGKSTVT
CCEEECCEEEECCCCEEECCHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCHHHH
TLVYEMAKAAGVKVGMGGNIGIPALSLLNEDCELYVLELSSFQLETTYSLKAAAATVLNV
HHHHHHHHHCCEEEECCCCCCCCHHHHCCCCCEEEEEEECCEEEEEEEEHHHHHHHHHHC
TEDHMDRYMDLEDYRQAKLRIYHNAKVGVLNNEDRLTFGENENQAKHTVSFAENSADYWL
CHHHHHHHCCHHHHHCEEEEEEECCEEEEECCCCCEEECCCCCCHHHEEEECCCCCCEEE
KTENGKQYLMVKDEVILPCEEATLVGRHNYMNILAATALAQAIGINLDSIRTALRHFKGL
EECCCCEEEEEECCEEECCCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
DHRFQLVHQANGIRWINDSKATNVGSTVAALAGLYIEGKLHLLLGGDGKGADFSELAELI
CHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCEEEECEEEEEECCCCCCCCHHHHHHHH
NQPHIICYCFGRDGALLAKFSSQSYLFDTMEQAIEFLRPTLQSGDMVLLSPACASLDQFA
CCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHH
SFEKRGEEFTHLAQCLT
HHHHHCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA