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The map label for this gene is rfaD [H]

Identifier: 16273039

GI number: 16273039

Start: 1180817

End: 1181743

Strand: Reverse

Name: rfaD [H]

Synonym: HI1114

Alternate gene names: 16273039

Gene position: 1181743-1180817 (Counterclockwise)

Preceding gene: 16273040

Following gene: 16273036

Centisome position: 64.57

GC content: 40.02

Gene sequence:

>927_bases
ATGATTATCGTAACAGGTGGCGCTGGTTTTATTGGCAGCAATATTGTCAAAGCATTGAATGATTTAGGACGCAAAGATAT
TTTAGTGGTCGATAACTTAAAAGATGGCACGAAATTTGCAAATTTAGTTGATTTAGACATCGCAGATTATTGTGATAAAG
AAGATTTCATTGCTTCTATTATCGCGGGCGATGAATTTGGCGATATTGATGCGGTATTCCACGAGGGGGCTTGTTCTGCG
ACTACGGAATGGGATGGCAAATACATTATGCACAACAACTACGAATATTCTAAAGAGTTGTTGCATTATTGCCTTGACCG
CGAAATTCCTTTTTTCTATGCCTCAAGCGCAGCAACTTATGGAGATACCAAAGTATTCCGTGAAGAACGTGAATTTGAAG
GCCCATTAAATGTGTATGGCTATTCTAAATTCTTGTTCGATCAATATGTGCGTAACATTTTGCCAGAAGCAAAATCGCCA
GTATGTGGTTTCCGTTATTTCAATGTTTACGGGCCGCGTGAAAATCATAAAGGTTCAATGGCGAGTGTGGCATTCCACTT
GAATAACCAAATCTTAAAAGGCGAAAATCCAAAATTATTTGCAGGCAGCGAACACTTCCGCCGTGATTTTGTTTATGTGG
GTGATGTGGCAGCAGTGAATATTTGGTGTTGGCAAAATGGTATTTCGGGTATTTACAACCTTGGTACAGGAAATGCAGAA
AGTTTCCGTGCGGTGGCTGATGCGGTAGTGAAATTCCACGGAAAAGGCGAGATTGAAACCATTCCATTCCCAGAGCATTT
GAAATCTCGTTATCAAGAATATACTCAAGCAGATTTAACTAAACTTCGCTCAACAGGCTACGATAAACCATTCAAAACAG
TTGCGGAAGGCGTGACTGAATATATGGCGTGGTTGAATAGAAAATAA

Upstream 100 bases:

>100_bases
TATTGGGGATTAAAAGTGCGGTTGTTTTTCGCAGAGTTTTTTGGCTAAGCGGTAGAAAATCTATTACAATACGCAGACTT
ATTTTAAAGAGGACAGAATT

Downstream 100 bases:

>100_bases
ATTATTAATGTAGTAAATCCCCTATAGCTTATCGGTATAGGGGATTTTATTTAATATAATTGTGATAAATGTGTTAAATA
ACGCTCATAAGCATTTTGAT

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MIIVTGGAGFIGSNIVKALNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDEFGDIDAVFHEGACSA
TTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATYGDTKVFREEREFEGPLNVYGYSKFLFDQYVRNILPEAKSP
VCGFRYFNVYGPRENHKGSMASVAFHLNNQILKGENPKLFAGSEHFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAE
SFRAVADAVVKFHGKGEIETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVTEYMAWLNRK

Sequences:

>Translated_308_residues
MIIVTGGAGFIGSNIVKALNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDEFGDIDAVFHEGACSA
TTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATYGDTKVFREEREFEGPLNVYGYSKFLFDQYVRNILPEAKSP
VCGFRYFNVYGPRENHKGSMASVAFHLNNQILKGENPKLFAGSEHFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAE
SFRAVADAVVKFHGKGEIETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVTEYMAWLNRK
>Mature_308_residues
MIIVTGGAGFIGSNIVKALNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDEFGDIDAVFHEGACSA
TTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATYGDTKVFREEREFEGPLNVYGYSKFLFDQYVRNILPEAKSP
VCGFRYFNVYGPRENHKGSMASVAFHLNNQILKGENPKLFAGSEHFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAE
SFRAVADAVVKFHGKGEIETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVTEYMAWLNRK

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790049, Length=308, Percent_Identity=78.2467532467532, Blast_Score=507, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71982035, Length=322, Percent_Identity=27.639751552795, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=324, Percent_Identity=27.4691358024691, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.20 [H]

Molecular weight: Translated: 34781; Mature: 34781

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVTGGAGFIGSNIVKALNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASI
CEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHEEECCHHHHCCHHHHHHHH
IAGDEFGDIDAVFHEGACSATTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATY
HCCCCCCCHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCC
GDTKVFREEREFEGPLNVYGYSKFLFDQYVRNILPEAKSPVCGFRYFNVYGPRENHKGSM
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCC
ASVAFHLNNQILKGENPKLFAGSEHFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAE
EEEEEEECCEEECCCCCCEEECCHHHHHCEEEECCEEEEEEEEECCCCCCEEECCCCCHH
SFRAVADAVVKFHGKGEIETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVTE
HHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YMAWLNRK
HHHHHCCC
>Mature Secondary Structure
MIIVTGGAGFIGSNIVKALNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASI
CEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHEEECCHHHHCCHHHHHHHH
IAGDEFGDIDAVFHEGACSATTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATY
HCCCCCCCHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCC
GDTKVFREEREFEGPLNVYGYSKFLFDQYVRNILPEAKSPVCGFRYFNVYGPRENHKGSM
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCC
ASVAFHLNNQILKGENPKLFAGSEHFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAE
EEEEEEECCEEECCCCCCEEECCHHHHHCEEEECCEEEEEEEEECCCCCCEEECCCCCHH
SFRAVADAVVKFHGKGEIETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVTE
HHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YMAWLNRK
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA