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The map label for this gene is xylF

Identifier: 16273036

GI number: 16273036

Start: 1176677

End: 1177675

Strand: Reverse

Name: xylF

Synonym: HI1111

Alternate gene names: 16273036

Gene position: 1177675-1176677 (Counterclockwise)

Preceding gene: 16273039

Following gene: 16273035

Centisome position: 64.35

GC content: 33.43

Gene sequence:

>999_bases
ATGAAAATCAAATCAGCTTTACTTACCCTTGTTGGTGCATTAACTGTATTTAGTAGTTCTGCCCATTCCAAAGATCTTAA
AATTGGTTTATCCATTGATGATTTACGTTTAGAAAGATGGCAAAAAGATCGAGATATTTTCGTAAACAAAGCAGAATCAA
TGGGTGCAAAAGTGTTTGTCCAATCTGCAAATGGTGATGATTCAGCACAAATTTCTCAAATCGAAAATATGATCAATAAA
AATATTGATGTATTAGTAATTATTCCTCATAACGGTGAAGTATTAAGTAACGTTATTTCTGAAGCTAAAAAGGAAGGAAT
AAAAGTATTAGCGTATGACCGTTTAATCAATAATGCAGATTTAGATTTTTATGTTTCCTTTGATAATGAAAAAGTGGGTG
AACTACAAGCTAAAAGTATTGTGGCTGTAAAACCAGAAGGTAATTATTTTTTAATGGGGGGATCTCCTGTAGATAATAAT
GCGAAATTATTTAGAAAAGGGCAAATGAAAGTATTAGATCCTTTAATTGCCAGTGGGAAAATTAAGGTTGTAGGAGATCA
ATGGGTTGATTCTTGGTTAGCTGAAAAAGCGTTACAAATTATGGAAAACGCATTAACAGCTAATAAAAATAATGTGGATG
CTGTTGTTGCATCTAACGATGCTACTGCTGGAGGTGCGATTCAAGCCCTTAGCGCACAAGGCTTATCTGGGAAAGTCGCA
ATTTCTGGGCAAGATGCAGATTTAGCTGCAATTAAACGTATTGTCAATGGCTCGCAAACTATGACTGTTTATAAACCAAT
TACGAAACTTGCAGATAAAGCGGCAGAAATTGCGGTTGAATTAGGTAAGAATGAAAAAATAGAAGCTAATGCAGAACTGA
ATAATGGCTTAAAAAATGTTCCTGCGTATTTACTTGATCCTATCGCTGTTGATAAACGTAATATTAATGAAACTGTAATT
AAAGATGGTTTTCATACTAAGGAAAGTATTTATCACTAA

Upstream 100 bases:

>100_bases
ATAATGGAGAAAGATTTTATAATTGAAAGCCTTTTTTAAAGTGTGAAAACTAACAACTGGAGCAATAACTAGTCCTTCAC
TTAACTATTGAGGTATCAAT

Downstream 100 bases:

>100_bases
CTTTAAGAGGGAAGAGAAAACTCTTCCCTTACCTCTTAGGGAGTTACGATATGGCATTGTTGGAAATGAAACATATCACA
AAAAAATTTGGTGATGTGAC

Product: D-xylose transporter subunit XylF

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKIKSALLTLVGALTVFSSSAHSKDLKIGLSIDDLRLERWQKDRDIFVNKAESMGAKVFVQSANGDDSAQISQIENMINK
NIDVLVIIPHNGEVLSNVISEAKKEGIKVLAYDRLINNADLDFYVSFDNEKVGELQAKSIVAVKPEGNYFLMGGSPVDNN
AKLFRKGQMKVLDPLIASGKIKVVGDQWVDSWLAEKALQIMENALTANKNNVDAVVASNDATAGGAIQALSAQGLSGKVA
ISGQDADLAAIKRIVNGSQTMTVYKPITKLADKAAEIAVELGKNEKIEANAELNNGLKNVPAYLLDPIAVDKRNINETVI
KDGFHTKESIYH

Sequences:

>Translated_332_residues
MKIKSALLTLVGALTVFSSSAHSKDLKIGLSIDDLRLERWQKDRDIFVNKAESMGAKVFVQSANGDDSAQISQIENMINK
NIDVLVIIPHNGEVLSNVISEAKKEGIKVLAYDRLINNADLDFYVSFDNEKVGELQAKSIVAVKPEGNYFLMGGSPVDNN
AKLFRKGQMKVLDPLIASGKIKVVGDQWVDSWLAEKALQIMENALTANKNNVDAVVASNDATAGGAIQALSAQGLSGKVA
ISGQDADLAAIKRIVNGSQTMTVYKPITKLADKAAEIAVELGKNEKIEANAELNNGLKNVPAYLLDPIAVDKRNINETVI
KDGFHTKESIYH
>Mature_332_residues
MKIKSALLTLVGALTVFSSSAHSKDLKIGLSIDDLRLERWQKDRDIFVNKAESMGAKVFVQSANGDDSAQISQIENMINK
NIDVLVIIPHNGEVLSNVISEAKKEGIKVLAYDRLINNADLDFYVSFDNEKVGELQAKSIVAVKPEGNYFLMGGSPVDNN
AKLFRKGQMKVLDPLIASGKIKVVGDQWVDSWLAEKALQIMENALTANKNNVDAVVASNDATAGGAIQALSAQGLSGKVA
ISGQDADLAAIKRIVNGSQTMTVYKPITKLADKAAEIAVELGKNEKIEANAELNNGLKNVPAYLLDPIAVDKRNINETVI
KDGFHTKESIYH

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family

Homologues:

Organism=Escherichia coli, GI1789990, Length=329, Percent_Identity=75.9878419452888, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1790192, Length=294, Percent_Identity=24.1496598639456, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790526, Length=290, Percent_Identity=25.8620689655172, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): XYLF_HAEIN (P45047)

Other databases:

- EMBL:   L42023
- PIR:   C64183
- RefSeq:   NP_439268.1
- ProteinModelPortal:   P45047
- GeneID:   950082
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1111
- NMPDR:   fig|71421.1.peg.1067
- TIGR:   HI_1111
- HOGENOM:   HBG531827
- OMA:   EAYTDGW
- ProtClustDB:   PRK10355
- BioCyc:   HINF71421:HI_1111-MONOMER
- InterPro:   IPR001761
- InterPro:   IPR013456
- TIGRFAMs:   TIGR02634

Pfam domain/function: PF00532 Peripla_BP_1

EC number: NA

Molecular weight: Translated: 35901; Mature: 35901

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKSALLTLVGALTVFSSSAHSKDLKIGLSIDDLRLERWQKDRDIFVNKAESMGAKVFV
CCHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHCCCCHHEEECHHCCCEEEEE
QSANGDDSAQISQIENMINKNIDVLVIIPHNGEVLSNVISEAKKEGIKVLAYDRLINNAD
EECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHCCCC
LDFYVSFDNEKVGELQAKSIVAVKPEGNYFLMGGSPVDNNAKLFRKGQMKVLDPLIASGK
EEEEEEECCCCCCCEEECEEEEECCCCCEEEECCCCCCCCHHHHCCCCHHHHHHHHCCCE
IKVVGDQWVDSWLAEKALQIMENALTANKNNVDAVVASNDATAGGAIQALSAQGLSGKVA
EEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCEEE
ISGQDADLAAIKRIVNGSQTMTVYKPITKLADKAAEIAVELGKNEKIEANAELNNGLKNV
ECCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHCCHHHC
PAYLLDPIAVDKRNINETVIKDGFHTKESIYH
CHHHHCCHHCCCCCCCHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MKIKSALLTLVGALTVFSSSAHSKDLKIGLSIDDLRLERWQKDRDIFVNKAESMGAKVFV
CCHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHCCCCHHEEECHHCCCEEEEE
QSANGDDSAQISQIENMINKNIDVLVIIPHNGEVLSNVISEAKKEGIKVLAYDRLINNAD
EECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHCCCC
LDFYVSFDNEKVGELQAKSIVAVKPEGNYFLMGGSPVDNNAKLFRKGQMKVLDPLIASGK
EEEEEEECCCCCCCEEECEEEEECCCCCEEEECCCCCCCCHHHHCCCCHHHHHHHHCCCE
IKVVGDQWVDSWLAEKALQIMENALTANKNNVDAVVASNDATAGGAIQALSAQGLSGKVA
EEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCEEE
ISGQDADLAAIKRIVNGSQTMTVYKPITKLADKAAEIAVELGKNEKIEANAELNNGLKNV
ECCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHCCHHHC
PAYLLDPIAVDKRNINETVIKDGFHTKESIYH
CHHHHCCHHCCCCCCCHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800