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The map label for this gene is nrfD

Identifier: 16272997

GI number: 16272997

Start: 1130062

End: 1131027

Strand: Reverse

Name: nrfD

Synonym: HI1066

Alternate gene names: 16272997

Gene position: 1131027-1130062 (Counterclockwise)

Preceding gene: 16272998

Following gene: 30995420

Centisome position: 61.8

GC content: 35.92

Gene sequence:

>966_bases
ATGACGTTAGATTATCCAGTTCCATTTCATACGCCTAATCTAGTGTGGGATTATACCATTGCGATCTACTTGTTTTTATT
GGGGATCTCATCTGGTGCGGTACAGTTAGCAATTGCCTATAAACGCAGTAATAAACTAGAAAATCTAAGCCAAAACTGGA
TTATTCGATCTGGGGTAATTTTAGGGTCTGTACCAACATTAATTGGTTTAACGCTATTAATTTTCCACTTAACTCGACCT
TGGACATTCTGGAAATTGATGTTTAACTATCAATTCAACTCCGTAATGTCGATGGGGGTCATGCTATTCCAAATCTATAT
GTTATTTTTAGTAATTTGGGGGGTAGTGATTTTCAAAAAAGAAATCGAAGCATTAATTAATCGCTTTATACCAAAACTGC
AATTTGTGATGAAACTCATCGGTATTGCAGAACGTATTGTAAGTCCAGTCGAAGTTATTCTTTTCATATTGGCTGCTGTG
CTTGGAGCATACACAGGGTTCTTACTTTCTGCATTGATTAGCTATCCAATGTTAAATAACCCAGTATTACCCGCATTATT
CTTAGCTTCTGGCACATCATCTGGTATTGCTGCAACCTTCTTAATTATCTTAATTGCAGGAAAACTAAAAGGTGATTCTC
ACGAATCGCACTTCATCCACAAATTTGAAGTGCCAATTATGGTGACAGAACTTGGTTTGATTGTGTGTTTCTTCGTTGGA
TTACATTTTGGTGGCGGACAAAAAACAGTTGCGTTACATAATGCCTTATCTGGTTTTTGGGGAGTGGTATTCTGGGTTGG
AGTATTGATTATTGGTATCTTGATCCCATTAATCGCCAATATGTTTGTAAATGACCGTTTAAAATACAATCGTAACTTTA
TTATCTTGGTATCAATTTTTGACTTAATTGGGGTATTTTGTTTACGTTTCTTTATTTTATATGCGGGACAACTCACTGTA
GCTTAA

Upstream 100 bases:

>100_bases
CACGTAAAGTGAAAGAAAAACCGGTTTATCGCACTAAAGTGGAATTAGGTACACAACCAAACTTATATCACATTCCATTC
CAACATGGGGAGCCTAGAAG

Downstream 100 bases:

>100_bases
ACTGAAATTTTATAAAAAATTAATCTGCATTCTAAAAGTTTGATTAACTTTCTCACTTATTAGAGTGCAGATTTTTATAT
CTAAATAATCCAACTATGAA

Product: nitrite reductase transmembrane protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTLDYPVPFHTPNLVWDYTIAIYLFLLGISSGAVQLAIAYKRSNKLENLSQNWIIRSGVILGSVPTLIGLTLLIFHLTRP
WTFWKLMFNYQFNSVMSMGVMLFQIYMLFLVIWGVVIFKKEIEALINRFIPKLQFVMKLIGIAERIVSPVEVILFILAAV
LGAYTGFLLSALISYPMLNNPVLPALFLASGTSSGIAATFLIILIAGKLKGDSHESHFIHKFEVPIMVTELGLIVCFFVG
LHFGGGQKTVALHNALSGFWGVVFWVGVLIIGILIPLIANMFVNDRLKYNRNFIILVSIFDLIGVFCLRFFILYAGQLTV
A

Sequences:

>Translated_321_residues
MTLDYPVPFHTPNLVWDYTIAIYLFLLGISSGAVQLAIAYKRSNKLENLSQNWIIRSGVILGSVPTLIGLTLLIFHLTRP
WTFWKLMFNYQFNSVMSMGVMLFQIYMLFLVIWGVVIFKKEIEALINRFIPKLQFVMKLIGIAERIVSPVEVILFILAAV
LGAYTGFLLSALISYPMLNNPVLPALFLASGTSSGIAATFLIILIAGKLKGDSHESHFIHKFEVPIMVTELGLIVCFFVG
LHFGGGQKTVALHNALSGFWGVVFWVGVLIIGILIPLIANMFVNDRLKYNRNFIILVSIFDLIGVFCLRFFILYAGQLTV
A
>Mature_320_residues
TLDYPVPFHTPNLVWDYTIAIYLFLLGISSGAVQLAIAYKRSNKLENLSQNWIIRSGVILGSVPTLIGLTLLIFHLTRPW
TFWKLMFNYQFNSVMSMGVMLFQIYMLFLVIWGVVIFKKEIEALINRFIPKLQFVMKLIGIAERIVSPVEVILFILAAVL
GAYTGFLLSALISYPMLNNPVLPALFLASGTSSGIAATFLIILIAGKLKGDSHESHFIHKFEVPIMVTELGLIVCFFVGL
HFGGGQKTVALHNALSGFWGVVFWVGVLIIGILIPLIANMFVNDRLKYNRNFIILVSIFDLIGVFCLRFFILYAGQLTVA

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family

Homologues:

Organism=Escherichia coli, GI1790509, Length=314, Percent_Identity=48.0891719745223, Blast_Score=277, Evalue=6e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFD_HAEIN (P45014)

Other databases:

- EMBL:   L42023
- PIR:   I64180
- RefSeq:   NP_439224.1
- GeneID:   950044
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1066
- NMPDR:   fig|71421.1.peg.1025
- TIGR:   HI_1066
- HOGENOM:   HBG458904
- OMA:   FWKIMIY
- ProtClustDB:   CLSK2518367
- BioCyc:   HINF71421:HI_1066-MONOMER
- InterPro:   IPR017566
- InterPro:   IPR005614
- TIGRFAMs:   TIGR03148

Pfam domain/function: PF03916 NrfD

EC number: NA

Molecular weight: Translated: 35945; Mature: 35814

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xdc71bb8)-; HASH(0xd16340c)-; HASH(0xdec7df4)-; HASH(0xd991c90)-; HASH(0xd164114)-; HASH(0xdf15f28)-; HASH(0xe244134)-; HASH(0xdade0e8)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDYPVPFHTPNLVWDYTIAIYLFLLGISSGAVQLAIAYKRSNKLENLSQNWIIRSGVI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHCCEEECCCH
LGSVPTLIGLTLLIFHLTRPWTFWKLMFNYQFNSVMSMGVMLFQIYMLFLVIWGVVIFKK
HCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIEALINRFIPKLQFVMKLIGIAERIVSPVEVILFILAAVLGAYTGFLLSALISYPMLNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVLPALFLASGTSSGIAATFLIILIAGKLKGDSHESHFIHKFEVPIMVTELGLIVCFFVG
CHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
LHFGGGQKTVALHNALSGFWGVVFWVGVLIIGILIPLIANMFVNDRLKYNRNFIILVSIF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCEEEHHHHH
DLIGVFCLRFFILYAGQLTVA
HHHHHHHHHHHHHHHCCCCCH
>Mature Secondary Structure 
TLDYPVPFHTPNLVWDYTIAIYLFLLGISSGAVQLAIAYKRSNKLENLSQNWIIRSGVI
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHCCEEECCCH
LGSVPTLIGLTLLIFHLTRPWTFWKLMFNYQFNSVMSMGVMLFQIYMLFLVIWGVVIFKK
HCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIEALINRFIPKLQFVMKLIGIAERIVSPVEVILFILAAVLGAYTGFLLSALISYPMLNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVLPALFLASGTSSGIAATFLIILIAGKLKGDSHESHFIHKFEVPIMVTELGLIVCFFVG
CHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
LHFGGGQKTVALHNALSGFWGVVFWVGVLIIGILIPLIANMFVNDRLKYNRNFIILVSIF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCEEEHHHHH
DLIGVFCLRFFILYAGQLTVA
HHHHHHHHHHHHHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800