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The map label for this gene is hindVM

Identifier: 16272974

GI number: 16272974

Start: 1105412

End: 1106326

Strand: Direct

Name: hindVM

Synonym: HI1041

Alternate gene names: 16272974

Gene position: 1105412-1106326 (Clockwise)

Preceding gene: 16272973

Following gene: 16272980

Centisome position: 60.4

GC content: 31.58

Gene sequence:

>915_bases
ATGAAGTGTGTAGACTTATTTTCAGGATGTGGAGGTTTATCTCTTGGTTTTGAATTAGCTGGATTTGAGATCTGTGCAGC
TTTTGAAAATTGGGAGAAAGCTATTGAGATTTATAAAAATAACTTTTCACACCCTATATATAATATTGATTTGAGAAATG
AGAAGGAAGCAGTCGAAAAGATTAAAAAATACAGTCCGGATTTAATAATGGGAGGTCCTCCATGTCAAGATTTTTCAAGT
GCGGGGAAGCGAGATATATCTTTGGGAAGAGCAGATTTAACATATAGTTTTGCAAATATAGTGTGTAACATTCGTCCAAA
ATGGTTTGTAATGGAGAATGTAGAACAAATTAAAAAAAGTCATATATTGCAAGATATTATTAATCAATTTATTGATTTTG
GTTATGGGTTGACTTCAGCTATATTAGATGCCTCTTACTGTGGTGTTCCACAATCTAGAACAAGATTTTCCTTAATTGGA
AAATTAAATAGTGAACATAATTTTCTGATTCCAACATTGTCTAGAAAATTATCAGATAAGCCTATGACAGTTAGAGATTA
TTTGGGAAATTCATTAAATCTAGAGTTTTATTATCGCCATCCAAGAAATTATAATAGAAGGGGAATTTTTTCAATCGATG
AGCCAAGTCCAACGATTAGAGGTGTAAATAGACCCATTCCTAAAGGATATAATATAAACAGTTGTGATCCTAAAGGAGTT
GAGTTAGCAAAAGTTAGACCATTAACAACAATTGAACGCAGTTATATTCAAACATTTCCTAAGTCGTTTTTATTTTCGGG
AACAAAAACTGATTTAGAGCAAATGATAGGTAATGCGGTACCAGTAAATTTAGCTAAATTTGTAGCTAGTGCAATTATAA
ACTTTGAAAAAGAACCAATAAGATCTATGGGATAA

Upstream 100 bases:

>100_bases
AGTCTGAAATAAAGAATATTATCTTAGGTGGAGGACAAGAATTACTTAGTCCAGAACGACGTTTTGATGCAATTATTTAC
AATTCTTCGGAGTTATTTAG

Downstream 100 bases:

>100_bases
ATTGATTTATTTGAAAATAAAGTATCTCCTTTTATTTTATCAGAGAAAGTGCTGCATAAGATAAAAGAACAGTATATATA
TCAATGATCTTTTATATTAA

Product: modification methylase

Products: NA

Alternate protein names: M.HindV; Cytosine-specific methyltransferase HindV

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKCVDLFSGCGGLSLGFELAGFEICAAFENWEKAIEIYKNNFSHPIYNIDLRNEKEAVEKIKKYSPDLIMGGPPCQDFSS
AGKRDISLGRADLTYSFANIVCNIRPKWFVMENVEQIKKSHILQDIINQFIDFGYGLTSAILDASYCGVPQSRTRFSLIG
KLNSEHNFLIPTLSRKLSDKPMTVRDYLGNSLNLEFYYRHPRNYNRRGIFSIDEPSPTIRGVNRPIPKGYNINSCDPKGV
ELAKVRPLTTIERSYIQTFPKSFLFSGTKTDLEQMIGNAVPVNLAKFVASAIINFEKEPIRSMG

Sequences:

>Translated_304_residues
MKCVDLFSGCGGLSLGFELAGFEICAAFENWEKAIEIYKNNFSHPIYNIDLRNEKEAVEKIKKYSPDLIMGGPPCQDFSS
AGKRDISLGRADLTYSFANIVCNIRPKWFVMENVEQIKKSHILQDIINQFIDFGYGLTSAILDASYCGVPQSRTRFSLIG
KLNSEHNFLIPTLSRKLSDKPMTVRDYLGNSLNLEFYYRHPRNYNRRGIFSIDEPSPTIRGVNRPIPKGYNINSCDPKGV
ELAKVRPLTTIERSYIQTFPKSFLFSGTKTDLEQMIGNAVPVNLAKFVASAIINFEKEPIRSMG
>Mature_304_residues
MKCVDLFSGCGGLSLGFELAGFEICAAFENWEKAIEIYKNNFSHPIYNIDLRNEKEAVEKIKKYSPDLIMGGPPCQDFSS
AGKRDISLGRADLTYSFANIVCNIRPKWFVMENVEQIKKSHILQDIINQFIDFGYGLTSAILDASYCGVPQSRTRFSLIG
KLNSEHNFLIPTLSRKLSDKPMTVRDYLGNSLNLEFYYRHPRNYNRRGIFSIDEPSPTIRGVNRPIPKGYNINSCDPKGV
ELAKVRPLTTIERSYIQTFPKSFLFSGTKTDLEQMIGNAVPVNLAKFVASAIINFEKEPIRSMG

Specific function: This methylase recognizes the double-stranded sequence GRCGYC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HindV endonuclease

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family

Homologues:

Organism=Homo sapiens, GI195927037, Length=190, Percent_Identity=30.5263157894737, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4503351, Length=190, Percent_Identity=30.5263157894737, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4758184, Length=170, Percent_Identity=28.2352941176471, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788271, Length=259, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTH5_HAEIN (P45000)

Other databases:

- EMBL:   L42023
- PIR:   C64109
- RefSeq:   NP_439200.1
- ProteinModelPortal:   P45000
- SMR:   P45000
- REBASE:   3574
- GeneID:   950661
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1041
- NMPDR:   fig|71421.1.peg.1002
- TIGR:   HI_1041
- HOGENOM:   HBG422323
- OMA:   RGVNRPI
- ProtClustDB:   CLSK892394
- BioCyc:   HINF71421:HI_1041-MONOMER
- BRENDA:   2.1.1.37
- InterPro:   IPR018117
- InterPro:   IPR001525
- PANTHER:   PTHR10629
- PRINTS:   PR00105
- TIGRFAMs:   TIGR00675

Pfam domain/function: PF00145 DNA_methylase

EC number: =2.1.1.37

Molecular weight: Translated: 34366; Mature: 34366

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00094 C5_MTASE_1; PS00095 C5_MTASE_2

Important sites: ACT_SITE 75-75

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCVDLFSGCGGLSLGFELAGFEICAAFENWEKAIEIYKNNFSHPIYNIDLRNEKEAVEK
CCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHH
IKKYSPDLIMGGPPCQDFSSAGKRDISLGRADLTYSFANIVCNIRPKWFVMENVEQIKKS
HHHCCCCEEECCCCCHHHHHCCCCCCCCCCHHHHEEHHHHEECCCCCEEEHHHHHHHHHH
HILQDIINQFIDFGYGLTSAILDASYCGVPQSRTRFSLIGKLNSEHNFLIPTLSRKLSDK
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHEECCCCCCCEECHHHHHHCCCC
PMTVRDYLGNSLNLEFYYRHPRNYNRRGIFSIDEPSPTIRGVNRPIPKGYNINSCDPKGV
CCCHHHHCCCCCEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
ELAKVRPLTTIERSYIQTFPKSFLFSGTKTDLEQMIGNAVPVNLAKFVASAIINFEKEPI
EEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHH
RSMG
HCCC
>Mature Secondary Structure
MKCVDLFSGCGGLSLGFELAGFEICAAFENWEKAIEIYKNNFSHPIYNIDLRNEKEAVEK
CCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHH
IKKYSPDLIMGGPPCQDFSSAGKRDISLGRADLTYSFANIVCNIRPKWFVMENVEQIKKS
HHHCCCCEEECCCCCHHHHHCCCCCCCCCCHHHHEEHHHHEECCCCCEEEHHHHHHHHHH
HILQDIINQFIDFGYGLTSAILDASYCGVPQSRTRFSLIGKLNSEHNFLIPTLSRKLSDK
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHEECCCCCCCEECHHHHHHCCCC
PMTVRDYLGNSLNLEFYYRHPRNYNRRGIFSIDEPSPTIRGVNRPIPKGYNINSCDPKGV
CCCHHHHCCCCCEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
ELAKVRPLTTIERSYIQTFPKSFLFSGTKTDLEQMIGNAVPVNLAKFVASAIINFEKEPI
EEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHH
RSMG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800