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The map label for this gene is tbpA

Identifier: 16272953

GI number: 16272953

Start: 1082773

End: 1083771

Strand: Direct

Name: tbpA

Synonym: HI1019

Alternate gene names: 16272953

Gene position: 1082773-1083771 (Clockwise)

Preceding gene: 16272951

Following gene: 16272954

Centisome position: 59.16

GC content: 37.94

Gene sequence:

>999_bases
ATGAAACTTCTTAAATTAACCTTAATTTCTACCGCACTTTTTTCCACCGCAGCATTAGCTCAAGCACAGCAAAGTGTAAA
CGTATATAGCTACGACTCTTTCACTTCTGAATGGGGGGCTGGGCCAAAAGTAAAACAAGATTTTGAAAAAGCCCATCCAC
AATGTGCGATTAATTTTACGCCTTTTGAAAGCGTTGGTGTGTTGCTCAACCGTGTTCGTTTGGAAGGAAAGAAAACAAAA
GCGGATATTGTGCTAGGTTTAGATAACTTTTTTTTGGAACAAGCTGAAAAAACAGGTATTTTTGCGCCAAATAACGTGGA
TTTAACTCAGCTGGATTTACCAACAAAATGGGCGAATAAAACCTTCTTACCTTTTGATTTCGGTAATTATGCTTTTGTGT
ACGATAAAACTAAATTACAAAACCCGCCGAAAAGTTTGAAAGAATTAGTTGAACGTCAAGATCTTAGTGTGATTTATCAA
GATCCTCGCACCAGCAGTGTGGGTCGTGGTTTATTGGTTTGGATGAATGCTGTTTACCCTGCTGATAAAATTCAATCCGC
TTGGAAAGAGTTAGATAAGCATACAGTGACAGTGGGTAAAGGCTGGTCAGATACTTACGGTGCATTTTTGAAAGGCGAAG
CGGATTTAGTATTAAGTTACAGCACATCGCCACTTTATCATCAACTTTTTGAGAAAAAAGATAATTATGCGGCGACAGAT
TTTGCGGAAGGTCATATTACTCAAGTAGAATTAGCGGCGCGAGTTGCAAACCATCCAAATCAATGTGCGGATGATTTTAT
GGCATTTTTAATTTCGCCTACAGCACAAAAACATATTGTGACCGCGAACATTATGTTGCCAGTTATTCAAGGCGAAATTG
AACCGCACTTTGATGCACTTAAAGTTCAACAAAAAACTCAAACGTCAATTAATCCAATGGTGAATACTGAGCAGTTAAAA
AATTGGATCAGCACTTGGCAAACTACATTAACAAAATAA

Upstream 100 bases:

>100_bases
GAACCTGAAACAGTTAATACTGACGTAGGAAACTAGGAATATAACCTTATCTCCTTTTCGTCATTATTGCTGCCTTTTAA
TCATAAATAAGGATGTAATA

Downstream 100 bases:

>100_bases
TTTGATGTTTTCGTTATTTCATCATCCACAACTTCGCCCTCGTCATTATGCGGGCGGGGTTGTTGTTATTAGTTTCATTA
TTCTTTTTTATGGTGGTGCC

Product: thiamin ABC transporter periplasmic-binding protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKLLKLTLISTALFSTAALAQAQQSVNVYSYDSFTSEWGAGPKVKQDFEKAHPQCAINFTPFESVGVLLNRVRLEGKKTK
ADIVLGLDNFFLEQAEKTGIFAPNNVDLTQLDLPTKWANKTFLPFDFGNYAFVYDKTKLQNPPKSLKELVERQDLSVIYQ
DPRTSSVGRGLLVWMNAVYPADKIQSAWKELDKHTVTVGKGWSDTYGAFLKGEADLVLSYSTSPLYHQLFEKKDNYAATD
FAEGHITQVELAARVANHPNQCADDFMAFLISPTAQKHIVTANIMLPVIQGEIEPHFDALKVQQKTQTSINPMVNTEQLK
NWISTWQTTLTK

Sequences:

>Translated_332_residues
MKLLKLTLISTALFSTAALAQAQQSVNVYSYDSFTSEWGAGPKVKQDFEKAHPQCAINFTPFESVGVLLNRVRLEGKKTK
ADIVLGLDNFFLEQAEKTGIFAPNNVDLTQLDLPTKWANKTFLPFDFGNYAFVYDKTKLQNPPKSLKELVERQDLSVIYQ
DPRTSSVGRGLLVWMNAVYPADKIQSAWKELDKHTVTVGKGWSDTYGAFLKGEADLVLSYSTSPLYHQLFEKKDNYAATD
FAEGHITQVELAARVANHPNQCADDFMAFLISPTAQKHIVTANIMLPVIQGEIEPHFDALKVQQKTQTSINPMVNTEQLK
NWISTWQTTLTK
>Mature_332_residues
MKLLKLTLISTALFSTAALAQAQQSVNVYSYDSFTSEWGAGPKVKQDFEKAHPQCAINFTPFESVGVLLNRVRLEGKKTK
ADIVLGLDNFFLEQAEKTGIFAPNNVDLTQLDLPTKWANKTFLPFDFGNYAFVYDKTKLQNPPKSLKELVERQDLSVIYQ
DPRTSSVGRGLLVWMNAVYPADKIQSAWKELDKHTVTVGKGWSDTYGAFLKGEADLVLSYSTSPLYHQLFEKKDNYAATD
FAEGHITQVELAARVANHPNQCADDFMAFLISPTAQKHIVTANIMLPVIQGEIEPHFDALKVQQKTQTSINPMVNTEQLK
NWISTWQTTLTK

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family

Homologues:

Organism=Escherichia coli, GI1786255, Length=313, Percent_Identity=51.7571884984025, Blast_Score=309, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIB_HAEIN (P44984)

Other databases:

- EMBL:   L42023
- PIR:   C64164
- RefSeq:   NP_439179.1
- ProteinModelPortal:   P44984
- SMR:   P44984
- GeneID:   949440
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1019
- NMPDR:   fig|71421.1.peg.981
- TIGR:   HI_1019
- HOGENOM:   HBG476839
- OMA:   AGPKVKQ
- ProtClustDB:   CLSK870073
- BioCyc:   HINF71421:HI_1019-MONOMER
- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967
- TIGRFAMs:   TIGR01254
- TIGRFAMs:   TIGR01276

Pfam domain/function: PF01547 SBP_bac_1

EC number: NA

Molecular weight: Translated: 37273; Mature: 37273

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLKLTLISTALFSTAALAQAQQSVNVYSYDSFTSEWGAGPKVKQDFEKAHPQCAINFT
CCEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCHHHHHHHCCCCEEEECC
PFESVGVLLNRVRLEGKKTKADIVLGLDNFFLEQAEKTGIFAPNNVDLTQLDLPTKWANK
CHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCCCCEEEECCCCHHCCC
TFLPFDFGNYAFVYDKTKLQNPPKSLKELVERQDLSVIYQDPRTSSVGRGLLVWMNAVYP
EECEEECCCEEEEEECHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHEEHHHHCCC
ADKIQSAWKELDKHTVTVGKGWSDTYGAFLKGEADLVLSYSTSPLYHQLFEKKDNYAATD
HHHHHHHHHHHHHCEEEECCCCCCCCCCEECCCCEEEEEECCCHHHHHHHHCCCCCCCCC
FAEGHITQVELAARVANHPNQCADDFMAFLISPTAQKHIVTANIMLPVIQGEIEPHFDAL
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCHHHH
KVQQKTQTSINPMVNTEQLKNWISTWQTTLTK
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLLKLTLISTALFSTAALAQAQQSVNVYSYDSFTSEWGAGPKVKQDFEKAHPQCAINFT
CCEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCHHHHHHHCCCCEEEECC
PFESVGVLLNRVRLEGKKTKADIVLGLDNFFLEQAEKTGIFAPNNVDLTQLDLPTKWANK
CHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCCCCEEEECCCCHHCCC
TFLPFDFGNYAFVYDKTKLQNPPKSLKELVERQDLSVIYQDPRTSSVGRGLLVWMNAVYP
EECEEECCCEEEEEECHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHEEHHHHCCC
ADKIQSAWKELDKHTVTVGKGWSDTYGAFLKGEADLVLSYSTSPLYHQLFEKKDNYAATD
HHHHHHHHHHHHHCEEEECCCCCCCCCCEECCCCEEEEEECCCHHHHHHHHCCCCCCCCC
FAEGHITQVELAARVANHPNQCADDFMAFLISPTAQKHIVTANIMLPVIQGEIEPHFDAL
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCHHHH
KVQQKTQTSINPMVNTEQLKNWISTWQTTLTK
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800