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The map label for this gene is vacB

Identifier: 16272801

GI number: 16272801

Start: 910379

End: 912727

Strand: Reverse

Name: vacB

Synonym: HI0861

Alternate gene names: 16272801

Gene position: 912727-910379 (Counterclockwise)

Preceding gene: 16272802

Following gene: 16272800

Centisome position: 49.87

GC content: 40.61

Gene sequence:

>2349_bases
ATGACAAAAAAACGTAAAAACTTAATAAACCAAGATCCGCATTACAAACGAGAATTGGAAAAATACGGCAATCCAATCCC
GAGCCGTGAATTTATTTTAACCGTTATTCGAGACAATAATGCACCGATGAATCGGGATGAAATTTTGACCGCACTTTCCA
TTCGAAATGAAGATCAAATAGAAGCGATGCGTCGCCGATTACGCGCGATGGAAAATGACGGACAATTAGTGTTCACCAAA
CGTAAACGCTATGCCTTACCAGAAAAATTAGATTTATTTAAAGGCACGGTAATTGGTCATCGCGAAGGTTTTGGTTTTTT
ACAGGTCGATGGCAAAAAGGATGATTTATTCATTCCGAACCATCAAATGCAAAGAGTCATGCATGGAGATTTTGTCTTAG
CGCAACCTGCTGGATTAGATCGTCGTGGTCGCCGTGAAGTGCGAATTGTGCGCGTATTAGAAAGCCGTAAAAAACAAATT
GTGGGTCGTTTTTTCTTAGAAAACGGTTTTGGCTATGTGGTGCCTGATGATAGCAGAATTGGTCGAGATATTCTCGTGCC
AAATGAACATCGTAATGGTGCACGTATGGGACAAGTTGTCGTTGTCGAATTGCAAGAACGTTCTGCCAGTTTTAACCAGC
CAATTGGCGTAATCACTGAAATCCTAGGCGATAATATGGCGAAAGGAATGGAAGTAGAAATCGCCTTGCGTAACCACGAT
ATTCTCCATAAGTTTCCAAGTGCGGTGGAAAAATATGTTAAAAAATTCACTGAAGAAGTCTCTGAAGAAGCAAAAAAAGG
CCGTGTGGATTTACGCAATTTACCGCTTGTGACCATTGATGGCGAAGATGCGCGGGATTTTGATGATGCCGTTTATTGTG
AAAAACACGGCAAAGGCTGGAAACTTTGGGTGGCGATTGCGGATGTTAGTTATTACGTACGTTTACGTTCCACTTTGGAT
GTAGAAGCGCATAATCGTGGTAACTCGGTCTATTTTCCGAACCGAGTTGTACCTATGCTACCCGAAATTTTATCGAACGG
CTTATGCTCTCTTAACCCACAAGTGGATCGTTTGTGTATGGTGTGTGAAATGCAAATTTCTGCCAAAGGTAAGCTCACAG
ATTATCGCTTTTATGAAGCAGTGATGAATTCTCACGCTCGCCTCACTTATACCAAAGTAGCAAAAATGCTTGAAGGCGAT
GAAGAATTACGCACTCGTTATTCTACGCTTGTGCCTCATTTAGAAGAACTTTATAAACTCTATCAAGCTTTGCTTAGTGC
GCGTCATCAACGTGGTGCGATCGATTTTGAAACCATTGAAACTAAGTTTATTTTTAATGCAATGGGGAGAATTGAGCGTA
TTGAACCCGTTGTTCGCAATGATGCCCAKAAAATCATTGAGGAATGTATGAWTCTTGCGAATATCGCAGCAGCCAATTTT
ATGGAAAAACATAAAGAGCCAGCGTTATATCGCATTCATGCCACCCCGAGCGAAGAAAAATTAACATCGTTCCGCACTTT
CTTAAGTGAGTTTGGTTTAACTTTAGAAGGCGGATTAAAACCAACTACAAAAGACTATGCTGCTCTCTTAGAAAAAGTTA
AAGAACGGCCTGATCATGAACTTATTCAAACAATGTTGCTACGTTCATTAAGTCAAGCCGTCTATCATGCTGATAATATT
GGGCACTTTGGCTTGGCTTTGGAAGAATATGCTCACTTTACTTCGCCAATTCGCCGTTATCCAGACTTAACACTTCATCG
AGGTATCAAATATTTACTCGCTAAAGAACAAGGCGCTAAACGTAAAACCACCGATACAGGCGGTTATCATTACTCTTTTG
ATGAAATGGATTTACTTGGCAATCACTGTTCAATGACCGAACGCCGCGCTGACGACGCAACTCGTGAAGTGGCTGATTGG
CTGAAATGTGAATATATGCAAGATCATGTCGGTGGCGAATTTAGCGGTGTTATTTCATCTGTGACGGGATTTGGTTTATT
TGTACGCTTAGATGATTTATTCATCGATGGTTTAGTCCATATTTCCACCTTAGAAAACGATTATTACCAATTCGATGCAG
CAAAACAGCGTTTAATCGGCGAAAATAGCGGAATGCAATATCGCTTAGGCGATAAAGTTCGCATCAAAGTTGAAGCGGTA
CATTTAGAAAATAAAATGGTCGATTTCTCGCTAATAGGTAGTGAACGTAAGCCTCGTCGAGCTGGAAAAACCGCGAAAGA
AAAAGCTAAGAAAGTTTTTAAAGAGCTACCGTCCAAAGCCTCCAAAAAACGAAAAAGTGCGGTAAAAAAGAAAGACGTTT
CGAAGAAAACATCTAGAAAACGAAAATAA

Upstream 100 bases:

>100_bases
TAAAATATCCTTAAAATATACAGTGATAATTTTATCCCCGTCTTGCATTATCCTCCTTACAGTGTCAAACTCTCCGCACT
TTTTAAAACTGTAAAAAATA

Downstream 100 bases:

>100_bases
TTCACTTCCTATTGTAAGAATGAATTAAAAATTCAAATAAGAAAAAGAGAAATTTATGTCAGAACAAATCTACGGCATTC
ATGCTGTTAATAGTATTTTA

Product: virulence-associated protein

Products: NA

Alternate protein names: RNase R; VacB protein homolog

Number of amino acids: Translated: 782; Mature: 781

Protein sequence:

>782_residues
MTKKRKNLINQDPHYKRELEKYGNPIPSREFILTVIRDNNAPMNRDEILTALSIRNEDQIEAMRRRLRAMENDGQLVFTK
RKRYALPEKLDLFKGTVIGHREGFGFLQVDGKKDDLFIPNHQMQRVMHGDFVLAQPAGLDRRGRREVRIVRVLESRKKQI
VGRFFLENGFGYVVPDDSRIGRDILVPNEHRNGARMGQVVVVELQERSASFNQPIGVITEILGDNMAKGMEVEIALRNHD
ILHKFPSAVEKYVKKFTEEVSEEAKKGRVDLRNLPLVTIDGEDARDFDDAVYCEKHGKGWKLWVAIADVSYYVRLRSTLD
VEAHNRGNSVYFPNRVVPMLPEILSNGLCSLNPQVDRLCMVCEMQISAKGKLTDYRFYEAVMNSHARLTYTKVAKMLEGD
EELRTRYSTLVPHLEELYKLYQALLSARHQRGAIDFETIETKFIFNAMGRIERIEPVVRNDAXKIIEECMXLANIAAANF
MEKHKEPALYRIHATPSEEKLTSFRTFLSEFGLTLEGGLKPTTKDYAALLEKVKERPDHELIQTMLLRSLSQAVYHADNI
GHFGLALEEYAHFTSPIRRYPDLTLHRGIKYLLAKEQGAKRKTTDTGGYHYSFDEMDLLGNHCSMTERRADDATREVADW
LKCEYMQDHVGGEFSGVISSVTGFGLFVRLDDLFIDGLVHISTLENDYYQFDAAKQRLIGENSGMQYRLGDKVRIKVEAV
HLENKMVDFSLIGSERKPRRAGKTAKEKAKKVFKELPSKASKKRKSAVKKKDVSKKTSRKRK

Sequences:

>Translated_782_residues
MTKKRKNLINQDPHYKRELEKYGNPIPSREFILTVIRDNNAPMNRDEILTALSIRNEDQIEAMRRRLRAMENDGQLVFTK
RKRYALPEKLDLFKGTVIGHREGFGFLQVDGKKDDLFIPNHQMQRVMHGDFVLAQPAGLDRRGRREVRIVRVLESRKKQI
VGRFFLENGFGYVVPDDSRIGRDILVPNEHRNGARMGQVVVVELQERSASFNQPIGVITEILGDNMAKGMEVEIALRNHD
ILHKFPSAVEKYVKKFTEEVSEEAKKGRVDLRNLPLVTIDGEDARDFDDAVYCEKHGKGWKLWVAIADVSYYVRLRSTLD
VEAHNRGNSVYFPNRVVPMLPEILSNGLCSLNPQVDRLCMVCEMQISAKGKLTDYRFYEAVMNSHARLTYTKVAKMLEGD
EELRTRYSTLVPHLEELYKLYQALLSARHQRGAIDFETIETKFIFNAMGRIERIEPVVRNDAXKIIEECMXLANIAAANF
MEKHKEPALYRIHATPSEEKLTSFRTFLSEFGLTLEGGLKPTTKDYAALLEKVKERPDHELIQTMLLRSLSQAVYHADNI
GHFGLALEEYAHFTSPIRRYPDLTLHRGIKYLLAKEQGAKRKTTDTGGYHYSFDEMDLLGNHCSMTERRADDATREVADW
LKCEYMQDHVGGEFSGVISSVTGFGLFVRLDDLFIDGLVHISTLENDYYQFDAAKQRLIGENSGMQYRLGDKVRIKVEAV
HLENKMVDFSLIGSERKPRRAGKTAKEKAKKVFKELPSKASKKRKSAVKKKDVSKKTSRKRK
>Mature_781_residues
TKKRKNLINQDPHYKRELEKYGNPIPSREFILTVIRDNNAPMNRDEILTALSIRNEDQIEAMRRRLRAMENDGQLVFTKR
KRYALPEKLDLFKGTVIGHREGFGFLQVDGKKDDLFIPNHQMQRVMHGDFVLAQPAGLDRRGRREVRIVRVLESRKKQIV
GRFFLENGFGYVVPDDSRIGRDILVPNEHRNGARMGQVVVVELQERSASFNQPIGVITEILGDNMAKGMEVEIALRNHDI
LHKFPSAVEKYVKKFTEEVSEEAKKGRVDLRNLPLVTIDGEDARDFDDAVYCEKHGKGWKLWVAIADVSYYVRLRSTLDV
EAHNRGNSVYFPNRVVPMLPEILSNGLCSLNPQVDRLCMVCEMQISAKGKLTDYRFYEAVMNSHARLTYTKVAKMLEGDE
ELRTRYSTLVPHLEELYKLYQALLSARHQRGAIDFETIETKFIFNAMGRIERIEPVVRNDAXKIIEECMXLANIAAANFM
EKHKEPALYRIHATPSEEKLTSFRTFLSEFGLTLEGGLKPTTKDYAALLEKVKERPDHELIQTMLLRSLSQAVYHADNIG
HFGLALEEYAHFTSPIRRYPDLTLHRGIKYLLAKEQGAKRKTTDTGGYHYSFDEMDLLGNHCSMTERRADDATREVADWL
KCEYMQDHVGGEFSGVISSVTGFGLFVRLDDLFIDGLVHISTLENDYYQFDAAKQRLIGENSGMQYRLGDKVRIKVEAVH
LENKMVDFSLIGSERKPRRAGKTAKEKAKKVFKELPSKASKKRKSAVKKKDVSKKTSRKRK

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI190014623, Length=443, Percent_Identity=31.6027088036117, Blast_Score=177, Evalue=5e-44,
Organism=Homo sapiens, GI190014625, Length=443, Percent_Identity=31.6027088036117, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI134288890, Length=506, Percent_Identity=28.2608695652174, Blast_Score=150, Evalue=6e-36,
Organism=Homo sapiens, GI19115966, Length=456, Percent_Identity=26.3157894736842, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI219521928, Length=456, Percent_Identity=26.3157894736842, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI156105695, Length=211, Percent_Identity=28.9099526066351, Blast_Score=78, Evalue=4e-14,
Organism=Homo sapiens, GI156105693, Length=211, Percent_Identity=28.9099526066351, Blast_Score=77, Evalue=8e-14,
Organism=Escherichia coli, GI87082383, Length=740, Percent_Identity=66.8918918918919, Blast_Score=1040, Evalue=0.0,
Organism=Escherichia coli, GI1787542, Length=660, Percent_Identity=27.4242424242424, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI212645896, Length=423, Percent_Identity=29.7872340425532, Blast_Score=166, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17553506, Length=464, Percent_Identity=28.8793103448276, Blast_Score=159, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324552, Length=568, Percent_Identity=24.8239436619718, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24649634, Length=476, Percent_Identity=28.9915966386555, Blast_Score=166, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24654592, Length=373, Percent_Identity=27.6139410187668, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19922976, Length=373, Percent_Identity=27.6139410187668, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24654597, Length=373, Percent_Identity=27.6139410187668, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNR_HAEIN (P44907)

Other databases:

- EMBL:   L42023
- PIR:   G64098
- RefSeq:   NP_439021.1
- ProteinModelPortal:   P44907
- GeneID:   949873
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0861
- NMPDR:   fig|71421.1.peg.829
- TIGR:   HI_0861
- HOGENOM:   HBG690243
- OMA:   GHRDGFG
- ProtClustDB:   CLSK870678
- BioCyc:   HINF71421:HI_0861-MONOMER
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805
- InterPro:   IPR013668
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR23355:SF9
- SMART:   SM00357
- SMART:   SM00955
- SMART:   SM00316
- TIGRFAMs:   TIGR00358
- TIGRFAMs:   TIGR02063

Pfam domain/function: PF08461 HTH_12; PF08206 OB_RNB; PF00773 RNB; PF00575 S1; SSF50249 Nucleic_acid_OB

EC number: 3.1.-.- [C]

Molecular weight: Translated: 89740; Mature: 89609

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS01175 RIBONUCLEASE_II; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKRKNLINQDPHYKRELEKYGNPIPSREFILTVIRDNNAPMNRDEILTALSIRNEDQI
CCCHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCHHHH
EAMRRRLRAMENDGQLVFTKRKRYALPEKLDLFKGTVIGHREGFGFLQVDGKKDDLFIPN
HHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCEEECCCCCEEEEECCCCCCEECCC
HQMQRVMHGDFVLAQPAGLDRRGRREVRIVRVLESRKKQIVGRFFLENGFGYVVPDDSRI
HHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
GRDILVPNEHRNGARMGQVVVVELQERSASFNQPIGVITEILGDNMAKGMEVEIALRNHD
CCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCHHHHHHHHCCHHCCCCEEEEEECCCH
ILHKFPSAVEKYVKKFTEEVSEEAKKGRVDLRNLPLVTIDGEDARDFDDAVYCEKHGKGW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEECCCCCCCCCCHHHHHCCCCCE
KLWVAIADVSYYVRLRSTLDVEAHNRGNSVYFPNRVVPMLPEILSNGLCSLNPQVDRLCM
EEEEEEECHHEEEEEHHHCCEEECCCCCEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHH
VCEMQISAKGKLTDYRFYEAVMNSHARLTYTKVAKMLEGDEELRTRYSTLVPHLEELYKL
HHHHHCCCCCCCCHHHHHHHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YQALLSARHQRGAIDFETIETKFIFNAMGRIERIEPVVRNDAXKIIEECMXLANIAAANF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
MEKHKEPALYRIHATPSEEKLTSFRTFLSEFGLTLEGGLKPTTKDYAALLEKVKERPDHE
HHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCHH
LIQTMLLRSLSQAVYHADNIGHFGLALEEYAHFTSPIRRYPDLTLHRGIKYLLAKEQGAK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
RKTTDTGGYHYSFDEMDLLGNHCSMTERRADDATREVADWLKCEYMQDHVGGEFSGVISS
CCCCCCCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VTGFGLFVRLDDLFIDGLVHISTLENDYYQFDAAKQRLIGENSGMQYRLGDKVRIKVEAV
HHHCEEEEEEHHHHHCCHHEEEECCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEEE
HLENKMVDFSLIGSERKPRRAGKTAKEKAKKVFKELPSKASKKRKSAVKKKDVSKKTSRK
EECCCEEEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RK
CC
>Mature Secondary Structure 
TKKRKNLINQDPHYKRELEKYGNPIPSREFILTVIRDNNAPMNRDEILTALSIRNEDQI
CCHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCHHHH
EAMRRRLRAMENDGQLVFTKRKRYALPEKLDLFKGTVIGHREGFGFLQVDGKKDDLFIPN
HHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCEEECCCCCEEEEECCCCCCEECCC
HQMQRVMHGDFVLAQPAGLDRRGRREVRIVRVLESRKKQIVGRFFLENGFGYVVPDDSRI
HHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
GRDILVPNEHRNGARMGQVVVVELQERSASFNQPIGVITEILGDNMAKGMEVEIALRNHD
CCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCHHHHHHHHCCHHCCCCEEEEEECCCH
ILHKFPSAVEKYVKKFTEEVSEEAKKGRVDLRNLPLVTIDGEDARDFDDAVYCEKHGKGW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEECCCCCCCCCCHHHHHCCCCCE
KLWVAIADVSYYVRLRSTLDVEAHNRGNSVYFPNRVVPMLPEILSNGLCSLNPQVDRLCM
EEEEEEECHHEEEEEHHHCCEEECCCCCEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHH
VCEMQISAKGKLTDYRFYEAVMNSHARLTYTKVAKMLEGDEELRTRYSTLVPHLEELYKL
HHHHHCCCCCCCCHHHHHHHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YQALLSARHQRGAIDFETIETKFIFNAMGRIERIEPVVRNDAXKIIEECMXLANIAAANF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
MEKHKEPALYRIHATPSEEKLTSFRTFLSEFGLTLEGGLKPTTKDYAALLEKVKERPDHE
HHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCHH
LIQTMLLRSLSQAVYHADNIGHFGLALEEYAHFTSPIRRYPDLTLHRGIKYLLAKEQGAK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
RKTTDTGGYHYSFDEMDLLGNHCSMTERRADDATREVADWLKCEYMQDHVGGEFSGVISS
CCCCCCCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VTGFGLFVRLDDLFIDGLVHISTLENDYYQFDAAKQRLIGENSGMQYRLGDKVRIKVEAV
HHHCEEEEEEHHHHHCCHHEEEECCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEEE
HLENKMVDFSLIGSERKPRRAGKTAKEKAKKVFKELPSKASKKRKSAVKKKDVSKKTSRK
EECCCEEEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800