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The map label for this gene is rlmB

Identifier: 16272800

GI number: 16272800

Start: 909583

End: 910323

Strand: Reverse

Name: rlmB

Synonym: HI0860

Alternate gene names: 16272800

Gene position: 910323-909583 (Counterclockwise)

Preceding gene: 16272801

Following gene: 16272796

Centisome position: 49.74

GC content: 41.97

Gene sequence:

>741_bases
ATGTCAGAACAAATCTACGGCATTCATGCTGTTAATAGTATTTTAACCCATTCACCAGAACGTTTAATTGAAGTCTTCGT
ACTTAAAGGCCGTGAAGATAAACGTCTACAGCCACTCTTAAATGAGCTTTATTCCCTGGGAATTGGTGTGCAATTTGTTA
ATCGACAAACACTTGATAAAAAAGCCGATGGTGAGGTTCATCAAGGTGTCATTGCTCGAGTACAAGCGGCAAAAGAACTT
AATGAAAATGATCTTGATGAAATTCTTGCGAATAAACAAAATCCGCTTTTATTAGTTTTGGATGGCGTCACAGATCCACA
CAATTTGGGAGCATGTTTGCGAACAGCGGATGCAGCGGGCGCAGTGGCAGTGATCGTACCGAAAGATAAATCCGCACAAT
TAACATCTATTGCACGTAAAGTTGCTTGTGGCGCGGCAGAAACCGTGCCGTTAATTCGTGTAACCAATCTTTCTCGCACA
CTACGCGATTTACAGCAAAACCATAATATTTGGGTGGTTGGCACTGCTGGCGAAGCAACTGAAACCATTTATCAAAGCAA
ACTGACAGGACCACTAGCACTTGTTATGGGAGCCGAAGGCGAAGGAATGCGTCGTTTAACACGTGAACATTGCGATCAAC
TGATTAGCATTCCAATGGCTGGTTCAGTTTCATCATTAAATGTATCTGTCGCAACAGGGGTATGTTTATTTGAAATTGTA
CGTCAGCGTTTAGGTTCATAA

Upstream 100 bases:

>100_bases
AAAAAGAAAGACGTTTCGAAGAAAACATCTAGAAAACGAAAATAATTCACTTCCTATTGTAAGAATGAATTAAAAATTCA
AATAAGAAAAAGAGAAATTT

Downstream 100 bases:

>100_bases
GAAAAGTGCGGTAAATTTACCGCACTTTTTTAATACCTATTGTCTCGCTTGAACTTCTGCGTTGGCATAATCAATGGTAA
CAACCTTACCAGGTAATAAC

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSEQIYGIHAVNSILTHSPERLIEVFVLKGREDKRLQPLLNELYSLGIGVQFVNRQTLDKKADGEVHQGVIARVQAAKEL
NENDLDEILANKQNPLLLVLDGVTDPHNLGACLRTADAAGAVAVIVPKDKSAQLTSIARKVACGAAETVPLIRVTNLSRT
LRDLQQNHNIWVVGTAGEATETIYQSKLTGPLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
RQRLGS

Sequences:

>Translated_246_residues
MSEQIYGIHAVNSILTHSPERLIEVFVLKGREDKRLQPLLNELYSLGIGVQFVNRQTLDKKADGEVHQGVIARVQAAKEL
NENDLDEILANKQNPLLLVLDGVTDPHNLGACLRTADAAGAVAVIVPKDKSAQLTSIARKVACGAAETVPLIRVTNLSRT
LRDLQQNHNIWVVGTAGEATETIYQSKLTGPLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
RQRLGS
>Mature_245_residues
SEQIYGIHAVNSILTHSPERLIEVFVLKGREDKRLQPLLNELYSLGIGVQFVNRQTLDKKADGEVHQGVIARVQAAKELN
ENDLDEILANKQNPLLLVLDGVTDPHNLGACLRTADAAGAVAVIVPKDKSAQLTSIARKVACGAAETVPLIRVTNLSRTL
RDLQQNHNIWVVGTAGEATETIYQSKLTGPLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIVR
QRLGS

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily

Homologues:

Organism=Homo sapiens, GI40068479, Length=232, Percent_Identity=32.3275862068966, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=72.4279835390947, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1788935, Length=241, Percent_Identity=28.6307053941909, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790083, Length=164, Percent_Identity=33.5365853658537, Blast_Score=67, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=258, Percent_Identity=27.906976744186, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324775, Length=175, Percent_Identity=30.2857142857143, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMB_HAEIN (P44906)

Other databases:

- EMBL:   L42023
- PIR:   E64160
- RefSeq:   NP_439020.1
- ProteinModelPortal:   P44906
- SMR:   P44906
- GeneID:   949872
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0860
- NMPDR:   fig|71421.1.peg.828
- TIGR:   HI_0860
- HOGENOM:   HBG714057
- OMA:   NELVILM
- ProtClustDB:   PRK11181
- BioCyc:   HINF71421:HI_0860-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01887
- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123
- SMART:   SM00967
- TIGRFAMs:   TIGR00186

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26602; Mature: 26471

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: BINDING 197-197 BINDING 217-217 BINDING 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQIYGIHAVNSILTHSPERLIEVFVLKGREDKRLQPLLNELYSLGIGVQFVNRQTLDK
CCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCHHHCCC
KADGEVHQGVIARVQAAKELNENDLDEILANKQNPLLLVLDGVTDPHNLGACLRTADAAG
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCC
AVAVIVPKDKSAQLTSIARKVACGAAETVPLIRVTNLSRTLRDLQQNHNIWVVGTAGEAT
EEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEEECCCHHH
ETIYQSKLTGPLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHH
RQRLGS
HHHHCC
>Mature Secondary Structure 
SEQIYGIHAVNSILTHSPERLIEVFVLKGREDKRLQPLLNELYSLGIGVQFVNRQTLDK
CCCEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCHHHCCC
KADGEVHQGVIARVQAAKELNENDLDEILANKQNPLLLVLDGVTDPHNLGACLRTADAAG
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCC
AVAVIVPKDKSAQLTSIARKVACGAAETVPLIRVTNLSRTLRDLQQNHNIWVVGTAGEAT
EEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEEECCCHHH
ETIYQSKLTGPLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHH
RQRLGS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023