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The map label for this gene is dcuB

Identifier: 16272687

GI number: 16272687

Start: 804454

End: 805776

Strand: Direct

Name: dcuB

Synonym: HI0746

Alternate gene names: 16272687

Gene position: 804454-805776 (Clockwise)

Preceding gene: 16272686

Following gene: 16272688

Centisome position: 43.96

GC content: 40.51

Gene sequence:

>1323_bases
ATGTCTGCAATGTTTTTACTACAATTTGCCATCGTATTATTGTGTATCTTTGTGGGTGCGCGTGTAGGTGGTATCGGTTT
AGGTGTATTTGGTGGTTTAGGTCTTGCCATTCTTTCTTTTGGCTTTGGCTTGAAACCAGCAGGTTTACCTATTGATGTTA
TGTTTATGATTATGGCTGTAGTGGCAGCCGCGGCAGCAATGCAAGCAGCTGGCGGTTTAGACTACATGATCAAAATCGCA
ACCAAAATCTTACGTCGTAATCCTAAACACATTACGTTTATTGCGCCAGCAGTAACTTGGTTATTCACTCTCTTAGCGGG
TACTGGTCACGTGGCATATTCTGTATTGCCAGTTATCGCTGAAGTGAGCCGTCAAAACGGTATCCGTCCTGAACGCCCAA
TGTCAATGGCGGTAATTGCATCACAATTTGCTATCGTAGCAAGCCCAATCGCTGCAGCGGTGGTGGCTGTGGTTGCTTAC
CTTGAGCCACAAGGTATTCATCTTGGCGATGTTTTAATGGTAACCATTCCTTCAACTATTCTAGGACTTTTCCTTGCTTG
CTTATTTGTTAATAAAATGGGTAAAGAATTAAAAGATGATCCAGAATATCAACGTCGTTTGAATGATCCAAAATATGCAG
ATATTTTTGCTTCTACCACTTCAGTCAAAGAAGTAGAAGTGAGCAAAACAGCTAAAATTTCCGTTTCTTTATTCTTATTT
GGTGCTTTACTTGTCGTGTTAATGGGTGCAGCTCCTTCTATTCGACCTGTTTTTGATGGAAAACCAATGGGAATGGCACA
TACCATTGAAATTATTATGCTTTCTATTGGTGCATTGATTATCTTAACTTGTAAACCAGATGGCACAGCGATTACCAAAG
GTTCTGTATTCCATGCAGGTATGCGTGCAGTAATCGCAATCTTCGGTATTGCTTGGTTAGGTGACACTTTAATGCAGGCA
CACATTACTGAAGTAAAAGAAATGGTGAAAGGCTTAGTGGAAACTGCACCATGGGCATTTGCTTTCGCTCTATTTGTACT
TTCAGTATTAGTAAACAGCCAAGGTGCAACAGTAGCGACATTGTTCCCTCTTGGTATCGCATTAGGTATCCCTGCACCTG
TATTAATCGGTGTATTTGTTGCTGTAAATGGTTACTTCTTCATCCCTAACTATGGTCCAATCATTGCATCTCTTGACTTC
GATACCACAGGTACAACCAAAATCGGTAAATACATTTTAAACCACAGCTTTATGCTTCCAGGTTTATTGAGCATGTTTTT
CTGCTTATTAATCGGTTTAGGTCTTTCGCACGTTATTCTTTAA

Upstream 100 bases:

>100_bases
TTATTGATCCAAGTTATGGAATCTCTACCTATTTGTTGCTATATTACACGCCGTTTCAACAAATAAAACACAGTGTTTTT
TATTAACCTAGGGGGGCAGT

Downstream 100 bases:

>100_bases
AATGTGATTCGATAACAAAGAACGGTGCTGAAATAGCACCGTTTTTGTTGTTCTAATATCAACTTAAAATAACTCAAAAA
AATAACCGCACTTTGATATA

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSAMFLLQFAIVLLCIFVGARVGGIGLGVFGGLGLAILSFGFGLKPAGLPIDVMFMIMAVVAAAAAMQAAGGLDYMIKIA
TKILRRNPKHITFIAPAVTWLFTLLAGTGHVAYSVLPVIAEVSRQNGIRPERPMSMAVIASQFAIVASPIAAAVVAVVAY
LEPQGIHLGDVLMVTIPSTILGLFLACLFVNKMGKELKDDPEYQRRLNDPKYADIFASTTSVKEVEVSKTAKISVSLFLF
GALLVVLMGAAPSIRPVFDGKPMGMAHTIEIIMLSIGALIILTCKPDGTAITKGSVFHAGMRAVIAIFGIAWLGDTLMQA
HITEVKEMVKGLVETAPWAFAFALFVLSVLVNSQGATVATLFPLGIALGIPAPVLIGVFVAVNGYFFIPNYGPIIASLDF
DTTGTTKIGKYILNHSFMLPGLLSMFFCLLIGLGLSHVIL

Sequences:

>Translated_440_residues
MSAMFLLQFAIVLLCIFVGARVGGIGLGVFGGLGLAILSFGFGLKPAGLPIDVMFMIMAVVAAAAAMQAAGGLDYMIKIA
TKILRRNPKHITFIAPAVTWLFTLLAGTGHVAYSVLPVIAEVSRQNGIRPERPMSMAVIASQFAIVASPIAAAVVAVVAY
LEPQGIHLGDVLMVTIPSTILGLFLACLFVNKMGKELKDDPEYQRRLNDPKYADIFASTTSVKEVEVSKTAKISVSLFLF
GALLVVLMGAAPSIRPVFDGKPMGMAHTIEIIMLSIGALIILTCKPDGTAITKGSVFHAGMRAVIAIFGIAWLGDTLMQA
HITEVKEMVKGLVETAPWAFAFALFVLSVLVNSQGATVATLFPLGIALGIPAPVLIGVFVAVNGYFFIPNYGPIIASLDF
DTTGTTKIGKYILNHSFMLPGLLSMFFCLLIGLGLSHVIL
>Mature_439_residues
SAMFLLQFAIVLLCIFVGARVGGIGLGVFGGLGLAILSFGFGLKPAGLPIDVMFMIMAVVAAAAAMQAAGGLDYMIKIAT
KILRRNPKHITFIAPAVTWLFTLLAGTGHVAYSVLPVIAEVSRQNGIRPERPMSMAVIASQFAIVASPIAAAVVAVVAYL
EPQGIHLGDVLMVTIPSTILGLFLACLFVNKMGKELKDDPEYQRRLNDPKYADIFASTTSVKEVEVSKTAKISVSLFLFG
ALLVVLMGAAPSIRPVFDGKPMGMAHTIEIIMLSIGALIILTCKPDGTAITKGSVFHAGMRAVIAIFGIAWLGDTLMQAH
ITEVKEMVKGLVETAPWAFAFALFVLSVLVNSQGATVATLFPLGIALGIPAPVLIGVFVAVNGYFFIPNYGPIIASLDFD
TTGTTKIGKYILNHSFMLPGLLSMFFCLLIGLGLSHVIL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family

Homologues:

Organism=Escherichia coli, GI1790580, Length=439, Percent_Identity=42.1412300683371, Blast_Score=314, Evalue=7e-87,
Organism=Escherichia coli, GI1790565, Length=390, Percent_Identity=41.7948717948718, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCUB_HAEIN (P44855)

Other databases:

- EMBL:   L42023
- PIR:   B64090
- RefSeq:   NP_438905.1
- GeneID:   950696
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0746
- TIGR:   HI_0746
- HOGENOM:   HBG297083
- OMA:   FTFLAGT
- ProtClustDB:   PRK09412
- BioCyc:   HINF71421:HI_0746-MONOMER
- InterPro:   IPR004668
- PIRSF:   PIRSF004539
- TIGRFAMs:   TIGR00770

Pfam domain/function: PF03605 DcuA_DcuB

EC number: NA

Molecular weight: Translated: 46558; Mature: 46427

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xcc49030)-; HASH(0xd5604fc)-; HASH(0xd4a0598)-; HASH(0xd4ae31c)-; HASH(0xd5ae434)-; HASH(0xd1b417c)-; HASH(0xd21687c)-; HASH(0xce52b10)-; HASH(0xd2ae1ec)-; HASH(0xc070b2c)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
SAMFLLQFAIVLLCIFVGARVGGIGLGVFGGLGLAILSFGFGLKPAGLPIDVMFMIMAV
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
VAAAAAMQAAGGLDYMIKIATKILRRNPKHITFIAPAVTWLFTLLAGTGHVAYSVLPVIA
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHH
EVSRQNGIRPERPMSMAVIASQFAIVASPIAAAVVAVVAYLEPQGIHLGDVLMVTIPSTI
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LGLFLACLFVNKMGKELKDDPEYQRRLNDPKYADIFASTTSVKEVEVSKTAKISVSLFLF
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHCCCCHHHEECCCHHHHHHHHHHH
GALLVVLMGAAPSIRPVFDGKPMGMAHTIEIIMLSIGALIILTCKPDGTAITKGSVFHAG
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCEEEEEEECCCCCEEECCHHHHHH
MRAVIAIFGIAWLGDTLMQAHITEVKEMVKGLVETAPWAFAFALFVLSVLVNSQGATVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEHH
LFPLGIALGIPAPVLIGVFVAVNGYFFIPNYGPIIASLDFDTTGTTKIGKYILNHSFMLP
HHHHHHHHCCCHHHHHHHHHHHCCEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHH
GLLSMFFCLLIGLGLSHVIL
HHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800