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The map label for this gene is proS [H]

Identifier: 16272670

GI number: 16272670

Start: 782566

End: 784284

Strand: Direct

Name: proS [H]

Synonym: HI0729

Alternate gene names: 16272670

Gene position: 782566-784284 (Clockwise)

Preceding gene: 16272669

Following gene: 16272676

Centisome position: 42.76

GC content: 43.05

Gene sequence:

>1719_bases
ATGCGTACAAGTCAATATTTATTTTCCACCCTAAAAGAAACGCCAAATGATGCTCAGGTTGTGAGCCACCAATTAATGTT
GCGTGCAGGTATGATTCGCCCAATGGCATCAGGTTTATATAACTGGCTACCGACGGGTATTCGCGTATTGAAAAAAGTGG
AAAAAGTTGTTCGCGAAGAGATGAACAAAGGTGGTGCCATTGAGGTATTAATGCCAGTTGTGCAACCTGCGGAATTATGG
GAAGAGTCTGGTCGTTGGGATCAATATGGCCCTGAATTGCTTCGTTTTGAAGACCGCGGAAACCGTAACTTTGTACTTGG
ACCAACTCACGAGGAAGTGATTACAGATTTAGTCCGCCGAGAAGTTTCTTCATACAAACAACTTCCACTTAATTTATATC
AAATTCAAACGAAATTCCGTGATGAAGTGCGCCCTCGTTTTGGTGTAATGCGTTCGCGTGAATTTATTATGAAAGATGCG
TATTCTTTCCACACTACCCAAGAAAGTTTGCAAGCAACCTATGATGTGATGTATCAAGTATACAGTAACATTTTTAACCG
TTTAGGCTTAGATTTCCGTGCGGTGCAAGCGGATACAGGTTCAATCGGGGGTTCAGCTTCTCACGAATTCCAAGTGTTAG
CTTCAAGCGGGGAAGATGATGTTGTGTTCTCAACGGAATCGGATTTTGCCGCGAATATTGAGCTTGCAGAAGCAATTGCT
ATAGGCGAACGCCAAGCTCCAACGGCTGAAATGTGTTTAGTTGATACACCAAATGCGAAAACTATTGCGGAGTTGGTGGA
GCAATTCAATTTACCAATCGAGAAAACCGTAAAAACTTTGATTGTGAAAGGTGCAGACGAAAATCAACCGCTTGTTGCGT
TGATTATTCGCGGCGACCATGAATTAAACGAAATCAAAGCACAAAAACACCCGTTAGTGGCAGATCCTCTTGAGTTTGCG
GACGAAACTGAAATCAAAGCGAAAATTGGTTCGGGCGTGGGATCGTTAGGCGCGGTAAATCTTAATATTCCTGCAATTAT
TGACCGCACTGTGGCGTTAATGTCTGATTTTAGTTGTGGTGCGAATATCGACGGCAAACACTACTTCAACGTGAACTGGG
AGCGTGATGTAGCAATACCAAAAGTATTTGATTTACGTAACGTAGTAGAAGGCGATCCTAGTCCAGATGGCAAAGGCACG
CTTCAAATCAAACGTGGTATTGAAGTGGGGCATATTTTCCAACTGGGTAAAAAATACTCTGAAGCGATGAAAGCGACCGT
TCAAGGCGAAGATGGTAAACCACTTGTGATGACGATGGGTTGCTACGGTATTGGTGTAACTCGTGTGGTGGCTTCAGCCA
TTGAGCAACACCATGATGAACGCGGTATCATCTGGCCATCAGACGAAATCGCCCCATTCACTGTGGCGATTGTGCCAATG
AACATGCACAAATCTGAAGCCGTGCAAAAATACGCTGAAGAACTTTACCGCACTTTACAATCGCAAGGCGTAGATGTGAT
TTTTGATGATCGTAAAGAACGCCCAGGTGTGATGTTTGCAGACATGGAACTTATCGGTGTACCGCATATGGTGGTAATCG
GTGAGAAAAACCTAGACAACGGTGAAATTGAATATAAGAATCGTCGTACCGGTGAAAAAGAAATGATTAGTAAAGATAAA
TTGCTCAGTGTTTTAAACGAAAAGTTAGGTAATCTCTAA

Upstream 100 bases:

>100_bases
GCAAAATAATGACTAAGTGTTAAAAGTGCGGTTAAAAATGACCGCACTTTTTGTTATACTTGCGCCAATTTTTTCAGTTC
TTAGAATTTAAGGTTAAACA

Downstream 100 bases:

>100_bases
TCAGTATTTTATTTTGTATATTGTGACAAAAGAGCGGTTAAAAATAGCCGCTCTTTTTGTTGATCTAACGTTTATATTGA
ACAATGTTTAATCCAAACTA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MRTSQYLFSTLKETPNDAQVVSHQLMLRAGMIRPMASGLYNWLPTGIRVLKKVEKVVREEMNKGGAIEVLMPVVQPAELW
EESGRWDQYGPELLRFEDRGNRNFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFIMKDA
YSFHTTQESLQATYDVMYQVYSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAIA
IGERQAPTAEMCLVDTPNAKTIAELVEQFNLPIEKTVKTLIVKGADENQPLVALIIRGDHELNEIKAQKHPLVADPLEFA
DETEIKAKIGSGVGSLGAVNLNIPAIIDRTVALMSDFSCGANIDGKHYFNVNWERDVAIPKVFDLRNVVEGDPSPDGKGT
LQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVVASAIEQHHDERGIIWPSDEIAPFTVAIVPM
NMHKSEAVQKYAEELYRTLQSQGVDVIFDDRKERPGVMFADMELIGVPHMVVIGEKNLDNGEIEYKNRRTGEKEMISKDK
LLSVLNEKLGNL

Sequences:

>Translated_572_residues
MRTSQYLFSTLKETPNDAQVVSHQLMLRAGMIRPMASGLYNWLPTGIRVLKKVEKVVREEMNKGGAIEVLMPVVQPAELW
EESGRWDQYGPELLRFEDRGNRNFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFIMKDA
YSFHTTQESLQATYDVMYQVYSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAIA
IGERQAPTAEMCLVDTPNAKTIAELVEQFNLPIEKTVKTLIVKGADENQPLVALIIRGDHELNEIKAQKHPLVADPLEFA
DETEIKAKIGSGVGSLGAVNLNIPAIIDRTVALMSDFSCGANIDGKHYFNVNWERDVAIPKVFDLRNVVEGDPSPDGKGT
LQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVVASAIEQHHDERGIIWPSDEIAPFTVAIVPM
NMHKSEAVQKYAEELYRTLQSQGVDVIFDDRKERPGVMFADMELIGVPHMVVIGEKNLDNGEIEYKNRRTGEKEMISKDK
LLSVLNEKLGNL
>Mature_572_residues
MRTSQYLFSTLKETPNDAQVVSHQLMLRAGMIRPMASGLYNWLPTGIRVLKKVEKVVREEMNKGGAIEVLMPVVQPAELW
EESGRWDQYGPELLRFEDRGNRNFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFIMKDA
YSFHTTQESLQATYDVMYQVYSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAIA
IGERQAPTAEMCLVDTPNAKTIAELVEQFNLPIEKTVKTLIVKGADENQPLVALIIRGDHELNEIKAQKHPLVADPLEFA
DETEIKAKIGSGVGSLGAVNLNIPAIIDRTVALMSDFSCGANIDGKHYFNVNWERDVAIPKVFDLRNVVEGDPSPDGKGT
LQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVVASAIEQHHDERGIIWPSDEIAPFTVAIVPM
NMHKSEAVQKYAEELYRTLQSQGVDVIFDDRKERPGVMFADMELIGVPHMVVIGEKNLDNGEIEYKNRRTGEKEMISKDK
LLSVLNEKLGNL

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=46.9026548672566, Blast_Score=230, Evalue=3e-60,
Organism=Escherichia coli, GI1786392, Length=571, Percent_Identity=75.4816112084063, Blast_Score=901, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=216, Percent_Identity=42.1296296296296, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI193203271, Length=93, Percent_Identity=35.4838709677419, Blast_Score=83, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320931, Length=219, Percent_Identity=36.986301369863, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6322112, Length=164, Percent_Identity=26.219512195122, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656200, Length=248, Percent_Identity=35.0806451612903, Blast_Score=175, Evalue=6e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63976; Mature: 63976

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQYLFSTLKETPNDAQVVSHQLMLRAGMIRPMASGLYNWLPTGIRVLKKVEKVVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNKGGAIEVLMPVVQPAELWEESGRWDQYGPELLRFEDRGNRNFVLGPTHEEVITDLVRR
HCCCCCEEEHHHHCCHHHHHHHCCCCCCCCCHHEEEECCCCCEEEECCCHHHHHHHHHHH
EVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFIMKDAYSFHTTQESLQATYDVMYQV
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAIA
HHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCHHHHHHHH
IGERQAPTAEMCLVDTPNAKTIAELVEQFNLPIEKTVKTLIVKGADENQPLVALIIRGDH
CCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCEEEEEEECCC
ELNEIKAQKHPLVADPLEFADETEIKAKIGSGVGSLGAVNLNIPAIIDRTVALMSDFSCG
HHHHHHHHCCCCCCCCHHHCCCCHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHCCCCC
ANIDGKHYFNVNWERDVAIPKVFDLRNVVEGDPSPDGKGTLQIKRGIEVGHIFQLGKKYS
CCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
EAMKATVQGEDGKPLVMTMGCYGIGVTRVVASAIEQHHDERGIIWPSDEIAPFTVAIVPM
HHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEE
NMHKSEAVQKYAEELYRTLQSQGVDVIFDDRKERPGVMFADMELIGVPHMVVIGEKNLDN
CCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCHHHCCCEEEEEECCCCCC
GEIEYKNRRTGEKEMISKDKLLSVLNEKLGNL
CCEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTSQYLFSTLKETPNDAQVVSHQLMLRAGMIRPMASGLYNWLPTGIRVLKKVEKVVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNKGGAIEVLMPVVQPAELWEESGRWDQYGPELLRFEDRGNRNFVLGPTHEEVITDLVRR
HCCCCCEEEHHHHCCHHHHHHHCCCCCCCCCHHEEEECCCCCEEEECCCHHHHHHHHHHH
EVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFIMKDAYSFHTTQESLQATYDVMYQV
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAIA
HHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCHHHHHHHH
IGERQAPTAEMCLVDTPNAKTIAELVEQFNLPIEKTVKTLIVKGADENQPLVALIIRGDH
CCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCEEEEEEECCC
ELNEIKAQKHPLVADPLEFADETEIKAKIGSGVGSLGAVNLNIPAIIDRTVALMSDFSCG
HHHHHHHHCCCCCCCCHHHCCCCHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHCCCCC
ANIDGKHYFNVNWERDVAIPKVFDLRNVVEGDPSPDGKGTLQIKRGIEVGHIFQLGKKYS
CCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
EAMKATVQGEDGKPLVMTMGCYGIGVTRVVASAIEQHHDERGIIWPSDEIAPFTVAIVPM
HHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEE
NMHKSEAVQKYAEELYRTLQSQGVDVIFDDRKERPGVMFADMELIGVPHMVVIGEKNLDN
CCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCHHHCCCEEEEEECCCCCC
GEIEYKNRRTGEKEMISKDKLLSVLNEKLGNL
CCEEECCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA