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The map label for this gene is tpiA [H]

Identifier: 16272620

GI number: 16272620

Start: 720263

End: 721054

Strand: Direct

Name: tpiA [H]

Synonym: HI0678

Alternate gene names: 16272620

Gene position: 720263-721054 (Clockwise)

Preceding gene: 16272617

Following gene: 16272624

Centisome position: 39.36

GC content: 42.8

Gene sequence:

>792_bases
ATGGCACGTCGTCCTTTAGTTATGGGTAACTGGAAATTAAACGGTAGCAAAGCATTCACGAAAGAATTAATCGAGGGATT
AAAAGCGGAATTACATGATGTAACAGGTTGTGATGTAGCAATTGCCCCTCCCGTTATGTATTTAGGTACGGCTGAAGCAG
CACTTTCTGGTTGTGGTTGCAGCTGTGGCGGTAAAAGTGTGATTCAATTAGGTGCACAAAACGTAGATATCAATGTGAAA
GGTGCATTTACTGGTGATATTTCAACTGAAATGTTAAAAGATTTCGGCGCAAAATATATCATTATTGGTCACTCTGAACG
CCGCACTTATCATAAAGAAAGCGACGAATTTGTGGCGAAAAAATTTGGTGCATTAAAAGAAGCAGGTTTAGTGCCAGTAT
TATGTATCGGAGAATCTGAAGCAGAAAACGAAGCAGGCAAAACAGAAGAAGTATGTGCGCGTCAAATTGATGCAGTCATC
AATGCATTAGGCGTAGAAGCATTCAACGGTGCAGTGATCGCATATGAACCAATCTGGGCAATTGGCACGGGTAAATCTGC
AACTCCAGCACAAGCGCAAGCAGTTCACGCATTTATCCGTGGTCACATCGCAGCGAAATCACAAGCCGTGGCAGAGCAAG
TAATTATTCAATACGGCGGTTCTGTAAATGATGCAAATGCCGCTGAATTATTTACTCAGCCAGACATCGATGGCGCATTA
GTGGGTGGCGCATCATTAAAAGCCCCTGCATTTGCAGTAATTGTTAAAGCAGCAGCTGCAGCGAAAAACTAA

Upstream 100 bases:

>100_bases
TCTGTGAGAATTTTTTTAATTTTATGATCTTCTGCAAAGAATAGGGGCGAAATTTTGGTATAGTAAGCAAGTTAATTTTA
TCAACTATGGAGAAACAAAA

Downstream 100 bases:

>100_bases
TTTTCAGTTAATTAGAATAACAACAAAAGTGCGGTAAATTTTTACCGCACTTTTTTTATATCTTATATTTTTGATGAATG
GGTTAGTAAGTGGTATCAAT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MARRPLVMGNWKLNGSKAFTKELIEGLKAELHDVTGCDVAIAPPVMYLGTAEAALSGCGCSCGGKSVIQLGAQNVDINVK
GAFTGDISTEMLKDFGAKYIIIGHSERRTYHKESDEFVAKKFGALKEAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
NALGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHAFIRGHIAAKSQAVAEQVIIQYGGSVNDANAAELFTQPDIDGAL
VGGASLKAPAFAVIVKAAAAAKN

Sequences:

>Translated_263_residues
MARRPLVMGNWKLNGSKAFTKELIEGLKAELHDVTGCDVAIAPPVMYLGTAEAALSGCGCSCGGKSVIQLGAQNVDINVK
GAFTGDISTEMLKDFGAKYIIIGHSERRTYHKESDEFVAKKFGALKEAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
NALGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHAFIRGHIAAKSQAVAEQVIIQYGGSVNDANAAELFTQPDIDGAL
VGGASLKAPAFAVIVKAAAAAKN
>Mature_262_residues
ARRPLVMGNWKLNGSKAFTKELIEGLKAELHDVTGCDVAIAPPVMYLGTAEAALSGCGCSCGGKSVIQLGAQNVDINVKG
AFTGDISTEMLKDFGAKYIIIGHSERRTYHKESDEFVAKKFGALKEAGLVPVLCIGESEAENEAGKTEEVCARQIDAVIN
ALGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHAFIRGHIAAKSQAVAEQVIIQYGGSVNDANAAELFTQPDIDGALV
GGASLKAPAFAVIVKAAAAAKN

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=257, Percent_Identity=45.136186770428, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI226529917, Length=257, Percent_Identity=45.136186770428, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1790353, Length=261, Percent_Identity=72.4137931034483, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17536593, Length=258, Percent_Identity=47.6744186046512, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320255, Length=259, Percent_Identity=44.4015444015444, Blast_Score=195, Evalue=7e-51,
Organism=Drosophila melanogaster, GI28572008, Length=258, Percent_Identity=47.2868217054264, Blast_Score=200, Evalue=9e-52,
Organism=Drosophila melanogaster, GI28572006, Length=258, Percent_Identity=47.2868217054264, Blast_Score=200, Evalue=9e-52,
Organism=Drosophila melanogaster, GI28572004, Length=258, Percent_Identity=47.2868217054264, Blast_Score=199, Evalue=1e-51,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 27287; Mature: 27156

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRPLVMGNWKLNGSKAFTKELIEGLKAELHDVTGCDVAIAPPVMYLGTAEAALSGCGC
CCCCCEEEECEEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCHHHHHHCCCC
SCGGKSVIQLGAQNVDINVKGAFTGDISTEMLKDFGAKYIIIGHSERRTYHKESDEFVAK
CCCCHHHHHCCCCCEEEEEEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHH
KFGALKEAGLVPVLCIGESEAENEAGKTEEVCARQIDAVINALGVEAFNGAVIAYEPIWA
HHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEE
IGTGKSATPAQAQAVHAFIRGHIAAKSQAVAEQVIIQYGGSVNDANAAELFTQPDIDGAL
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEE
VGGASLKAPAFAVIVKAAAAAKN
ECCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARRPLVMGNWKLNGSKAFTKELIEGLKAELHDVTGCDVAIAPPVMYLGTAEAALSGCGC
CCCCEEEECEEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCHHHHHHCCCC
SCGGKSVIQLGAQNVDINVKGAFTGDISTEMLKDFGAKYIIIGHSERRTYHKESDEFVAK
CCCCHHHHHCCCCCEEEEEEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHH
KFGALKEAGLVPVLCIGESEAENEAGKTEEVCARQIDAVINALGVEAFNGAVIAYEPIWA
HHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEE
IGTGKSATPAQAQAVHAFIRGHIAAKSQAVAEQVIIQYGGSVNDANAAELFTQPDIDGAL
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEE
VGGASLKAPAFAVIVKAAAAAKN
ECCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA