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The map label for this gene is fmt [H]

Identifier: 16272566

GI number: 16272566

Start: 663211

End: 664167

Strand: Direct

Name: fmt [H]

Synonym: HI0623

Alternate gene names: 16272566

Gene position: 663211-664167 (Clockwise)

Preceding gene: 16272565

Following gene: 16272567

Centisome position: 36.24

GC content: 38.66

Gene sequence:

>957_bases
ATGAAATCACTTAATATTATTTTTGCTGGTACGCCAGATTTTGCTGCACAGCATTTACAAGCCATTCTAAATTCTCAACA
TAATGTGATCGCTGTTTATACTCAACCTGATAAACCAGCTGGTCGCGGTAAGAAATTGCAAGCAAGTCCTGTAAAGCAAC
TTGCTGAGCAAAATAACATTCCCGTTTATCAACCTAAATCCTTACGTAAAGAAGAAGCACAGTCTGAATTAAAAGCGTTA
AATGCAGATGTAATGGTTGTTGTGGCTTATGGATTAATTTTACCGAAAGCTGTATTAGATGCCCCCCGTTTGGGTTGTTT
GAATGTGCATGGTTCTATTCTTCCACGTTGGCGAGGTGCAGCACCAATTCAGCGTTCAATTTGGGCTGGCGATGTACAAA
CGGGTGTAACCATTATGCAAATGGATGAAGGTTTAGATACAGGCGATATGTTACATAAAGTCTATTGTGATATTTTACCG
ACTGAAACTTCAACGAGTCTTTATAACAAACTGGCAGAGCTTGCTCCATCAGCATTAATCGATGTTTTAGATAATCTTGA
AAACGGTAAATTTATAGCGGAAAAACAAGATGGCAGCCAAAGTAATTATGCAGAAAAACTTTCTAAAGAAGAGGCGCAAT
TAAATTGGTCACTTTCTGCAATGCAACTTGAGCGTAATATCCGCGCTTTTAATCCTTGGCCAATTGCCTATTTTTCAACA
GAAGACAAGGATGGCAATGCACACACTTTAAAAGTGTATCAAGCGAAAGTGTTGCCTCATCAAGATAAACCAGCGGGAAC
TATTTTAAGTGCGGATAAAAATGGCATTCAAATTGCGACTGTCGATGGCGTCTTAAACTTATTGCAATTACAATCTGCAG
GTAAAAAGCCTATGTCTGCACAAGATTTATTAAATGGCCGTGCAGAATGGTTTACTATTGGTAAGGTGCTTGCATAA

Upstream 100 bases:

>100_bases
AAAGCTAAAACGTAGGGTGGGCTTTAGCCAGCAATCATTTCAAAAAACGGTGGGCTTAAGCCCACCCTACTTCCTATCAA
TTACCATAACGTAGAACATT

Downstream 100 bases:

>100_bases
TGAAAAAATTCTCTTCTAAAACTATCAAAGCAAAAAATTCAGTAAAAATGACCGCACTTTCGACCAGGGCTATTGCTGCA
AACTTAATTTTGCAAGTATT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKSLNIIFAGTPDFAAQHLQAILNSQHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQNNIPVYQPKSLRKEEAQSELKAL
NADVMVVVAYGLILPKAVLDAPRLGCLNVHGSILPRWRGAAPIQRSIWAGDVQTGVTIMQMDEGLDTGDMLHKVYCDILP
TETSTSLYNKLAELAPSALIDVLDNLENGKFIAEKQDGSQSNYAEKLSKEEAQLNWSLSAMQLERNIRAFNPWPIAYFST
EDKDGNAHTLKVYQAKVLPHQDKPAGTILSADKNGIQIATVDGVLNLLQLQSAGKKPMSAQDLLNGRAEWFTIGKVLA

Sequences:

>Translated_318_residues
MKSLNIIFAGTPDFAAQHLQAILNSQHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQNNIPVYQPKSLRKEEAQSELKAL
NADVMVVVAYGLILPKAVLDAPRLGCLNVHGSILPRWRGAAPIQRSIWAGDVQTGVTIMQMDEGLDTGDMLHKVYCDILP
TETSTSLYNKLAELAPSALIDVLDNLENGKFIAEKQDGSQSNYAEKLSKEEAQLNWSLSAMQLERNIRAFNPWPIAYFST
EDKDGNAHTLKVYQAKVLPHQDKPAGTILSADKNGIQIATVDGVLNLLQLQSAGKKPMSAQDLLNGRAEWFTIGKVLA
>Mature_318_residues
MKSLNIIFAGTPDFAAQHLQAILNSQHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQNNIPVYQPKSLRKEEAQSELKAL
NADVMVVVAYGLILPKAVLDAPRLGCLNVHGSILPRWRGAAPIQRSIWAGDVQTGVTIMQMDEGLDTGDMLHKVYCDILP
TETSTSLYNKLAELAPSALIDVLDNLENGKFIAEKQDGSQSNYAEKLSKEEAQLNWSLSAMQLERNIRAFNPWPIAYFST
EDKDGNAHTLKVYQAKVLPHQDKPAGTILSADKNGIQIATVDGVLNLLQLQSAGKKPMSAQDLLNGRAEWFTIGKVLA

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI21614513, Length=242, Percent_Identity=28.9256198347107, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI238814322, Length=242, Percent_Identity=29.3388429752066, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI164663775, Length=236, Percent_Identity=32.2033898305085, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1789683, Length=317, Percent_Identity=64.3533123028391, Blast_Score=409, Evalue=1e-116,
Organism=Escherichia coli, GI1788589, Length=301, Percent_Identity=26.578073089701, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI133930964, Length=326, Percent_Identity=25.7668711656442, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319458, Length=280, Percent_Identity=26.0714285714286, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45550868, Length=318, Percent_Identity=31.4465408805031, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI28571984, Length=241, Percent_Identity=34.0248962655602, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24585660, Length=327, Percent_Identity=25.3822629969419, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34789; Mature: 34789

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLNIIFAGTPDFAAQHLQAILNSQHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQNNI
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
PVYQPKSLRKEEAQSELKALNADVMVVVAYGLILPKAVLDAPRLGCLNVHGSILPRWRGA
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
APIQRSIWAGDVQTGVTIMQMDEGLDTGDMLHKVYCDILPTETSTSLYNKLAELAPSALI
CCHHHHHCCCCHHHCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
DVLDNLENGKFIAEKQDGSQSNYAEKLSKEEAQLNWSLSAMQLERNIRAFNPWPIAYFST
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEEC
EDKDGNAHTLKVYQAKVLPHQDKPAGTILSADKNGIQIATVDGVLNLLQLQSAGKKPMSA
CCCCCCEEEEEEEEEEECCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCCCH
QDLLNGRAEWFTIGKVLA
HHHHCCCHHEEECCHHCC
>Mature Secondary Structure
MKSLNIIFAGTPDFAAQHLQAILNSQHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQNNI
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
PVYQPKSLRKEEAQSELKALNADVMVVVAYGLILPKAVLDAPRLGCLNVHGSILPRWRGA
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
APIQRSIWAGDVQTGVTIMQMDEGLDTGDMLHKVYCDILPTETSTSLYNKLAELAPSALI
CCHHHHHCCCCHHHCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
DVLDNLENGKFIAEKQDGSQSNYAEKLSKEEAQLNWSLSAMQLERNIRAFNPWPIAYFST
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEEC
EDKDGNAHTLKVYQAKVLPHQDKPAGTILSADKNGIQIATVDGVLNLLQLQSAGKKPMSA
CCCCCCEEEEEEEEEEECCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCCCH
QDLLNGRAEWFTIGKVLA
HHHHCCCHHEEECCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA