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The map label for this gene is potE

Identifier: 16272534

GI number: 16272534

Start: 611212

End: 612519

Strand: Reverse

Name: potE

Synonym: HI0590

Alternate gene names: 16272534

Gene position: 612519-611212 (Counterclockwise)

Preceding gene: 16272535

Following gene: 30995387

Centisome position: 33.47

GC content: 39.53

Gene sequence:

>1308_bases
ATGAGTGCTAAAAGCAATAAAATTGGTGTGGTACAACTCACCATTCTTACCATGGTTAATATGATGGGTTCTGGTATCAT
TATGTTACCGACCAAATTAGCCGAAATTGGAACCATTTCAATAGTGTCTTGGCTGGTCACAGCGGTTGGCTCAACTGCAT
TAGCATATGCATTTGCCCAATGTGGAATGTTTAGCAAAAAATCTGGTGGTATGGGCGGTTATGCGGAATACTCTTTCGGT
AAAGCCGGTAACTTCATGGCAAACTACACTTACGGTGTATCATTAGTTATTGCCAATACAGCAATCGCAATTTCTGCGGT
GGGTTATGGTTCAGAGCTTTTCGGCACAATCCTTTCACCACTTTCTATCGCATTATGGACTATCTTCACCCTTTGGCTTG
CAACCGTTCTTAACTTTGGTGGTGCAAGAATCACCGGTAATATCAGTTCATTTACCATTTGGGGCGTAATTATTCCTGTG
GTAGGTATTTCTATCATTGGTTGGAAATGGTTTGATGGTTCAATGTATGTAAACTCTTGGAACCCGCACAATGTACCAAC
TTTTGAAGCGATCGGTGTATCTATTTCAATGACATTATGGGCGTTCTTAGGGTTAGAATCAGCTTGTGCTAACGCAGATG
CCGTTGAAAATCCAGAGAAAAACGTACCAATCGCAGTACTTGGTGGTACCTTAGGAGCTGCGGTAATTTATATCGTTTCA
ACTAACGTGATTGCAGGTATTGTACCTAACCTTGAACTCGCAAATTCAACAGCGCCATTCGGTTTAGCCTTTGCTCATAT
GTTTGATGAAACTGTCGGTAAAGTTATCATGGGCTTAATGGTAATGTCTTGCTTTGGTTCATTATTAGGCTGGCAGTTCA
CTATTGCTCAAGTATTCAAATCTTCAGCAGAAGAAGGTTATTTCCCAGCATTTTTCAAAAAAATTACCAGTAAAGATGCA
CCTGTTGTTGGTATGATTACCATTACTGCGTTACAGACTTTACTCTCTTTAATGACAATCAGCCCATCATTAAATAAACA
ATTCAATGTGTTGGTGGACTTAGCAGTGGTTACAAACGTCATCCCATATTTGCTCTCAATGGCTGCTCTCGCAGTGTTAT
TGAAAGCGGAAAATGTTGCGCCACAAAAATATAAAACAACCGTTTTTGTTGCCTTTATTGGTTCACTCTATAGTATCTAT
GCACTTTATGCAGCCGGTGAACAAGCGATGTTATATGGTTCAATTGTGACCTTTATTGGTTGGACATTGTATGGCTTTGT
TTCTTACAAATTTGATCTTAAAAAATAA

Upstream 100 bases:

>100_bases
GTGAGGTTGTGAGGATCTTCTTCATAACTCTCTCAACACAAACAAAAAAGTGCGGTTAAAAATATCTGATTTTTTGACCG
CACTTGTAGGAGTATAAATT

Downstream 100 bases:

>100_bases
AAAAGAAACGCCTACATCTTAATGTAGGCTCTTTCGAGTAGTAATAAGCTAAGTACAAAAGAGCGGTTGAAAATTTAATT
GTTTTCAACCGCTCTTTTTA

Product: putrescine transporter

Products: Proton [Cytoplasm]; putrescine [Cytoplasm] [C]

Alternate protein names: Putrescine transport protein

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSAKSNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISIVSWLVTAVGSTALAYAFAQCGMFSKKSGGMGGYAEYSFG
KAGNFMANYTYGVSLVIANTAIAISAVGYGSELFGTILSPLSIALWTIFTLWLATVLNFGGARITGNISSFTIWGVIIPV
VGISIIGWKWFDGSMYVNSWNPHNVPTFEAIGVSISMTLWAFLGLESACANADAVENPEKNVPIAVLGGTLGAAVIYIVS
TNVIAGIVPNLELANSTAPFGLAFAHMFDETVGKVIMGLMVMSCFGSLLGWQFTIAQVFKSSAEEGYFPAFFKKITSKDA
PVVGMITITALQTLLSLMTISPSLNKQFNVLVDLAVVTNVIPYLLSMAALAVLLKAENVAPQKYKTTVFVAFIGSLYSIY
ALYAAGEQAMLYGSIVTFIGWTLYGFVSYKFDLKK

Sequences:

>Translated_435_residues
MSAKSNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISIVSWLVTAVGSTALAYAFAQCGMFSKKSGGMGGYAEYSFG
KAGNFMANYTYGVSLVIANTAIAISAVGYGSELFGTILSPLSIALWTIFTLWLATVLNFGGARITGNISSFTIWGVIIPV
VGISIIGWKWFDGSMYVNSWNPHNVPTFEAIGVSISMTLWAFLGLESACANADAVENPEKNVPIAVLGGTLGAAVIYIVS
TNVIAGIVPNLELANSTAPFGLAFAHMFDETVGKVIMGLMVMSCFGSLLGWQFTIAQVFKSSAEEGYFPAFFKKITSKDA
PVVGMITITALQTLLSLMTISPSLNKQFNVLVDLAVVTNVIPYLLSMAALAVLLKAENVAPQKYKTTVFVAFIGSLYSIY
ALYAAGEQAMLYGSIVTFIGWTLYGFVSYKFDLKK
>Mature_434_residues
SAKSNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISIVSWLVTAVGSTALAYAFAQCGMFSKKSGGMGGYAEYSFGK
AGNFMANYTYGVSLVIANTAIAISAVGYGSELFGTILSPLSIALWTIFTLWLATVLNFGGARITGNISSFTIWGVIIPVV
GISIIGWKWFDGSMYVNSWNPHNVPTFEAIGVSISMTLWAFLGLESACANADAVENPEKNVPIAVLGGTLGAAVIYIVST
NVIAGIVPNLELANSTAPFGLAFAHMFDETVGKVIMGLMVMSCFGSLLGWQFTIAQVFKSSAEEGYFPAFFKKITSKDAP
VVGMITITALQTLLSLMTISPSLNKQFNVLVDLAVVTNVIPYLLSMAALAVLLKAENVAPQKYKTTVFVAFIGSLYSIYA
LYAAGEQAMLYGSIVTFIGWTLYGFVSYKFDLKK

Specific function: Probable putrescine-ornithine antiporter

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily

Homologues:

Organism=Escherichia coli, GI1786908, Length=434, Percent_Identity=77.1889400921659, Blast_Score=682, Evalue=0.0,
Organism=Escherichia coli, GI2367353, Length=419, Percent_Identity=34.8448687350835, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1790575, Length=408, Percent_Identity=30.8823529411765, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1787890, Length=426, Percent_Identity=25.8215962441315, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786602, Length=220, Percent_Identity=25.9090909090909, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17540018, Length=326, Percent_Identity=26.6871165644172, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45550968, Length=330, Percent_Identity=24.8484848484848, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921172, Length=330, Percent_Identity=24.8484848484848, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): POTE_HAEIN (P44768)

Other databases:

- EMBL:   L42023
- PIR:   E64079
- RefSeq:   NP_438748.1
- GeneID:   950609
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0590
- NMPDR:   fig|71421.1.peg.561
- TIGR:   HI_0590
- HOGENOM:   HBG586921
- OMA:   YSIYALY
- ProtClustDB:   PRK10655
- BioCyc:   HINF71421:HI_0590-MONOMER
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004754
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060
- TIGRFAMs:   TIGR00905

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 46349; Mature: 46218

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xcc4bee4)-; HASH(0xd0b684c)-; HASH(0xd0a78b8)-; HASH(0xaff1d80)-; HASH(0xcbec0d4)-; HASH(0xd04b2bc)-; HASH(0xcadf480)-; HASH(0xce9f610)-; HASH(0xcbb5f94)-; HASH(0xb982c20)-; HASH(0xc8f829c)-; HASH(0x405a15d4)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKSNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISIVSWLVTAVGSTALAYAFAQ
CCCCCCCCEEEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CGMFSKKSGGMGGYAEYSFGKAGNFMANYTYGVSLVIANTAIAISAVGYGSELFGTILSP
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEHHEEEEEECCCCHHHHHHHHHH
LSIALWTIFTLWLATVLNFGGARITGNISSFTIWGVIIPVVGISIIGWKWFDGSMYVNSW
HHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHEEEEEECCEEEEECC
NPHNVPTFEAIGVSISMTLWAFLGLESACANADAVENPEKNVPIAVLGGTLGAAVIYIVS
CCCCCCCHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHEE
TNVIAGIVPNLELANSTAPFGLAFAHMFDETVGKVIMGLMVMSCFGSLLGWQFTIAQVFK
HHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSAEEGYFPAFFKKITSKDAPVVGMITITALQTLLSLMTISPSLNKQFNVLVDLAVVTNV
CCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IPYLLSMAALAVLLKAENVAPQKYKTTVFVAFIGSLYSIYALYAAGEQAMLYGSIVTFIG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
WTLYGFVSYKFDLKK
HHHHHHHEEEEECCH
>Mature Secondary Structure 
SAKSNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISIVSWLVTAVGSTALAYAFAQ
CCCCCCCEEEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CGMFSKKSGGMGGYAEYSFGKAGNFMANYTYGVSLVIANTAIAISAVGYGSELFGTILSP
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEHHEEEEEECCCCHHHHHHHHHH
LSIALWTIFTLWLATVLNFGGARITGNISSFTIWGVIIPVVGISIIGWKWFDGSMYVNSW
HHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHEEEEEECCEEEEECC
NPHNVPTFEAIGVSISMTLWAFLGLESACANADAVENPEKNVPIAVLGGTLGAAVIYIVS
CCCCCCCHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHEE
TNVIAGIVPNLELANSTAPFGLAFAHMFDETVGKVIMGLMVMSCFGSLLGWQFTIAQVFK
HHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSAEEGYFPAFFKKITSKDAPVVGMITITALQTLLSLMTISPSLNKQFNVLVDLAVVTNV
CCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IPYLLSMAALAVLLKAENVAPQKYKTTVFVAFIGSLYSIYALYAAGEQAMLYGSIVTFIG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
WTLYGFVSYKFDLKK
HHHHHHHEEEEECCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; putrescine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + putrescine [Periplasm] = Proton [Cytoplasm] + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800