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The map label for this gene is ksgA [H]

Identifier: 16272493

GI number: 16272493

Start: 569786

End: 570649

Strand: Direct

Name: ksgA [H]

Synonym: HI0549

Alternate gene names: 16272493

Gene position: 569786-570649 (Clockwise)

Preceding gene: 16272487

Following gene: 16272495

Centisome position: 31.13

GC content: 41.2

Gene sequence:

>864_bases
ATGAACTCAAAAAAACACTTAGGCCATACGGCGCGTAAACGCTTTGGTCAAAACTTTTTACATGATACTTCTGTCATTCA
AGGTATCGTGGCGGCGATTTACCCACAACCTAATCAATTCTTAGTGGAAATTGGCCCAGGGCTTGGTGCATTAACAGAGC
CTGTTGGTGAACTTGTCGATCATCTGACTGTGGTAGAGCTTGACCGAGACCTTGCAGAACGTTTACGTCACCATCCATTT
TTACATCAAAAGCTCACCGTTATTGAAACGGATGCGATGCAATTTGATTTTGGTGCGCTTTACACAAAAGAAAATTTAGC
AGAAAAAGGGCAAAAATTACGTGTGTTTGGTAACTTACCTTACAACATTTCTACGCCATTGATGTTCCACTTGTTTAAAT
ATCATGATGTGATTCAAGATATGCACTTTATGCTGCAAAAAGAAGTGGTAAAACGCCTATGTGCCGCACCAAATAGCAAA
GCTTATGGTCGTTTAACGATTATGGCGCAATATTTCTGCCAAGTGATGCCTGTGCTTGAAGTACCGCCTTCGGCATTCAA
ACCTGCACCGAAAGTAGATTCAGCCGTTGTGCGTTTAATTCCGCATAAGGAATTGCCGCACCCTGTAAAAGATTTATATT
GGTTGAACCGTGTTTGCTCCCAAGCGTTCAATCAACGTCGTAAAACATTACGCAATGCACTCTCAACTCTATTTTCACCT
GAAAATCTGACCGCACTTGGTATTGATTTAAATGCACGTGCAGAAAATTTGGCTATCGCAGATTATGCACGTTTAGCGAA
CTGGTTAGCGGATAATCCACCAGCAGATGTCAATAAAGATGAAATCTTAGATTCAGAAGAGTAA

Upstream 100 bases:

>100_bases
ATATTGTTCATGAAAAACTCAATTTGGCATAACCTAACAATGATTTACAAAGCTAAAACAACCCCTATAATAACCGCACT
TTTCTTATTAAATAGTACTT

Downstream 100 bases:

>100_bases
AAATAATAAAAAGGTGTAACCCTTGTTACACCTTGGCTTTCTACATAAAACGAACAATTTCTTTACCTTGCATTTCTTTA
ATACGTTGTTGTTCTTTCCA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNSKKHLGHTARKRFGQNFLHDTSVIQGIVAAIYPQPNQFLVEIGPGLGALTEPVGELVDHLTVVELDRDLAERLRHHPF
LHQKLTVIETDAMQFDFGALYTKENLAEKGQKLRVFGNLPYNISTPLMFHLFKYHDVIQDMHFMLQKEVVKRLCAAPNSK
AYGRLTIMAQYFCQVMPVLEVPPSAFKPAPKVDSAVVRLIPHKELPHPVKDLYWLNRVCSQAFNQRRKTLRNALSTLFSP
ENLTALGIDLNARAENLAIADYARLANWLADNPPADVNKDEILDSEE

Sequences:

>Translated_287_residues
MNSKKHLGHTARKRFGQNFLHDTSVIQGIVAAIYPQPNQFLVEIGPGLGALTEPVGELVDHLTVVELDRDLAERLRHHPF
LHQKLTVIETDAMQFDFGALYTKENLAEKGQKLRVFGNLPYNISTPLMFHLFKYHDVIQDMHFMLQKEVVKRLCAAPNSK
AYGRLTIMAQYFCQVMPVLEVPPSAFKPAPKVDSAVVRLIPHKELPHPVKDLYWLNRVCSQAFNQRRKTLRNALSTLFSP
ENLTALGIDLNARAENLAIADYARLANWLADNPPADVNKDEILDSEE
>Mature_287_residues
MNSKKHLGHTARKRFGQNFLHDTSVIQGIVAAIYPQPNQFLVEIGPGLGALTEPVGELVDHLTVVELDRDLAERLRHHPF
LHQKLTVIETDAMQFDFGALYTKENLAEKGQKLRVFGNLPYNISTPLMFHLFKYHDVIQDMHFMLQKEVVKRLCAAPNSK
AYGRLTIMAQYFCQVMPVLEVPPSAFKPAPKVDSAVVRLIPHKELPHPVKDLYWLNRVCSQAFNQRRKTLRNALSTLFSP
ENLTALGIDLNARAENLAIADYARLANWLADNPPADVNKDEILDSEE

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=294, Percent_Identity=29.9319727891156, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI7657198, Length=226, Percent_Identity=30.0884955752212, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1786236, Length=275, Percent_Identity=68, Blast_Score=386, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25141369, Length=290, Percent_Identity=29.3103448275862, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI25146882, Length=221, Percent_Identity=33.4841628959276, Blast_Score=98, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=205, Percent_Identity=30.2439024390244, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21357273, Length=257, Percent_Identity=30.3501945525292, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21358017, Length=240, Percent_Identity=32.0833333333333, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 32526; Mature: 32526

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
>Mature Secondary Structure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CCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA