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The map label for this gene is comE

Identifier: 16272383

GI number: 16272383

Start: 456967

End: 458304

Strand: Reverse

Name: comE

Synonym: HI0435

Alternate gene names: 16272383

Gene position: 458304-456967 (Counterclockwise)

Preceding gene: 16272384

Following gene: 16272382

Centisome position: 25.04

GC content: 37.67

Gene sequence:

>1338_bases
ATGAAGAAATATTTTTTAAAGTGCGGTTATTTTTTAGTATGTTTTTGTTTGCCATTAATCGTTTTTGCTAATCCTAAAAC
AGATAACGAACGTTTTTTTATTCGTTTATCGCAAGCACCTTTAGCTCAAACACTGGAGCAATTAGCTTTTCAACAAGATG
TGAATTTAGTGATTGGAGATATATTGGAAAACAAGATCTCTTTGAAATTAAACAATATTGATATGCCACGTTTGCTACAA
ATAATCGCAAAAAGTAAGCATCTTACTTTGAATAAAGATGATGGGATTTATTATTTAAACGGCAGTCAATCTGGCAAAGG
TCAGGTTGCAGGAAATCTTACGACAAATGAACCGCACTTAGTGAGTCACACGGTAAAACTCCATTTTGCTAAAGCTTCTG
AATTAATGAAATCCTTAACAACAGGAAGTGGCTCTTTGCTTTCTCCCGCTGGGAGCATTACCTTTGATGATCGCAGTAAT
TTGCTGGTTATTCAGGATGAACCTCGTTCTGTGCAAAATATCAAAAAACTGATTGCTGAAATGGATAAGCCTATTGAACA
GATCGCTATTGAAGCGCGAATTGTGACAATTACGGATGAGAGTTTGAAAGAACTTGGCGTTCGGTGGGGGATTTTTAATC
CAACTGAAAATGCAAGACGAGTTGCGGGCAGCCTTACAGGCAATAGCTTTGAAAATATTGCGGATAATCTTAATGTAAAT
TTTGCGACAACGACGACACCTGCTGGCTCTATAGCATTACAAGTCGCCAAAATTAATGGGCGATTGCTTGATTTAGAATT
GAGTGCGTTGGAGCGTGAAAATAATGTAGAAATTATTGCAAGCCCTCGCTTACTCACTACCAATAAGAAAAGTGCGAGCA
TTAAACAGGGGACAGAAATTCCTTACATCGTGAGTAATACTCGTAACGATACGCAATCTGTGGAATTTCGTGAGGCGGTG
CTTGGTTTGGAAGTGACGCCACATATTTCTAAAGATAACAATATCTTACTTGATTTATTGGTAAGTCAAAATTCCCCTGG
TTCTCGTGTCGCTTATGGACAAAATGAGGTGGTTTCTATTGATAAACAAGAAATTAATACTCAGGTTTTTGCCAAAGATG
GGGAAACCATTGTGCTTGGCGGCGTATTTCACGATACAATCACGAAAAGCGAAGATAAAGTGCCATTGCTTGGCGATATA
CCCGTTATTAAACGATTATTTAGCAAAGAAAGTGAACGACATCAAAAACGTGAGCTAGTGATTTTCGTCACGCCACATAT
TTTAAAAGCAGGAGAAACGTTAGAGGCGTTGAAACAAAAAAGTGAGGGTAAAAAATAA

Upstream 100 bases:

>100_bases
TGTAGAAAAAATCAACCAAAACAATGTTCAATTTATGCGTAAGCTAGGAGAGCAATGTGATAGTAGTGAATGGAAAAAAT
TAAGTTTTTAAAGGAAGATT

Downstream 100 bases:

>100_bases
CTTTTTAAATGATGAATTTTTTTAATTTTCGCTGTATCCACTGTCGTGGCAATCTTCATATCGCAAAAAATGGGTTATGT
TCAGGTTGCCAAAAACAAAT

Product: competence protein E

Products: NA

Alternate protein names: DNA transformation protein ComE

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKKYFLKCGYFLVCFCLPLIVFANPKTDNERFFIRLSQAPLAQTLEQLAFQQDVNLVIGDILENKISLKLNNIDMPRLLQ
IIAKSKHLTLNKDDGIYYLNGSQSGKGQVAGNLTTNEPHLVSHTVKLHFAKASELMKSLTTGSGSLLSPAGSITFDDRSN
LLVIQDEPRSVQNIKKLIAEMDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEIPYIVSNTRNDTQSVEFREAV
LGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSIDKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDI
PVIKRLFSKESERHQKRELVIFVTPHILKAGETLEALKQKSEGKK

Sequences:

>Translated_445_residues
MKKYFLKCGYFLVCFCLPLIVFANPKTDNERFFIRLSQAPLAQTLEQLAFQQDVNLVIGDILENKISLKLNNIDMPRLLQ
IIAKSKHLTLNKDDGIYYLNGSQSGKGQVAGNLTTNEPHLVSHTVKLHFAKASELMKSLTTGSGSLLSPAGSITFDDRSN
LLVIQDEPRSVQNIKKLIAEMDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEIPYIVSNTRNDTQSVEFREAV
LGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSIDKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDI
PVIKRLFSKESERHQKRELVIFVTPHILKAGETLEALKQKSEGKK
>Mature_445_residues
MKKYFLKCGYFLVCFCLPLIVFANPKTDNERFFIRLSQAPLAQTLEQLAFQQDVNLVIGDILENKISLKLNNIDMPRLLQ
IIAKSKHLTLNKDDGIYYLNGSQSGKGQVAGNLTTNEPHLVSHTVKLHFAKASELMKSLTTGSGSLLSPAGSITFDDRSN
LLVIQDEPRSVQNIKKLIAEMDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEIPYIVSNTRNDTQSVEFREAV
LGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSIDKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDI
PVIKRLFSKESERHQKRELVIFVTPHILKAGETLEALKQKSEGKK

Specific function: Involved in transformation (competence for DNA uptake)

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family

Homologues:

Organism=Escherichia coli, GI1789793, Length=436, Percent_Identity=37.1559633027523, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI87082242, Length=337, Percent_Identity=28.1899109792285, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COME_HAEIN (P31772)

Other databases:

- EMBL:   M62809
- EMBL:   L42023
- EMBL:   M59751
- PIR:   H64067
- RefSeq:   NP_438596.1
- GeneID:   950687
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0435
- NMPDR:   fig|71421.1.peg.409
- TIGR:   HI_0435
- HOGENOM:   HBG482665
- OMA:   ITMELNE
- ProtClustDB:   CLSK870404
- BioCyc:   HINF71421:HI_0435-MONOMER
- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR004846
- InterPro:   IPR004845
- InterPro:   IPR001814
- PRINTS:   PR00811
- PRINTS:   PR01032
- TIGRFAMs:   TIGR02515

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N

EC number: NA

Molecular weight: Translated: 49209; Mature: 49209

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYFLKCGYFLVCFCLPLIVFANPKTDNERFFIRLSQAPLAQTLEQLAFQQDVNLVIGD
CCCEEHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHH
ILENKISLKLNNIDMPRLLQIIAKSKHLTLNKDDGIYYLNGSQSGKGQVAGNLTTNEPHL
HHCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCEEEECCCCCCCEE
VSHTVKLHFAKASELMKSLTTGSGSLLSPAGSITFDDRSNLLVIQDEPRSVQNIKKLIAE
EEEEEEEEEHHHHHHHHHHCCCCCCEECCCCCEEECCCCCEEEEECCCCHHHHHHHHHHH
MDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
HCCCHHHEEEEEEEEEECHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCEE
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEI
EEEECCCCCCEEEEEEEECCEEEEEEHHHHCCCCCEEEEECCEEEECCCCCCCCCCCCCC
PYIVSNTRNDTQSVEFREAVLGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSI
CEEEECCCCCCHHHHHHHHHHCEEECCCCCCCCCEEEEEEEECCCCCCEEECCCCCEEEE
DKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDIPVIKRLFSKESERHQKRELV
CHHHCCEEEEECCCCEEEECCEEHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCEE
IFVTPHILKAGETLEALKQKSEGKK
EEECCHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKYFLKCGYFLVCFCLPLIVFANPKTDNERFFIRLSQAPLAQTLEQLAFQQDVNLVIGD
CCCEEHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHH
ILENKISLKLNNIDMPRLLQIIAKSKHLTLNKDDGIYYLNGSQSGKGQVAGNLTTNEPHL
HHCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCEEEECCCCCCCEE
VSHTVKLHFAKASELMKSLTTGSGSLLSPAGSITFDDRSNLLVIQDEPRSVQNIKKLIAE
EEEEEEEEEHHHHHHHHHHCCCCCCEECCCCCEEECCCCCEEEEECCCCHHHHHHHHHHH
MDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
HCCCHHHEEEEEEEEEECHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCEE
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEI
EEEECCCCCCEEEEEEEECCEEEEEEHHHHCCCCCEEEEECCEEEECCCCCCCCCCCCCC
PYIVSNTRNDTQSVEFREAVLGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSI
CEEEECCCCCCHHHHHHHHHHCEEECCCCCCCCCEEEEEEEECCCCCCEEECCCCCEEEE
DKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDIPVIKRLFSKESERHQKRELV
CHHHCCEEEEECCCCEEEECCEEHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCEE
IFVTPHILKAGETLEALKQKSEGKK
EEECCHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1916268; 7542800; 1856167