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The map label for this gene is yfiF [C]

Identifier: 16272372

GI number: 16272372

Start: 446149

End: 447204

Strand: Reverse

Name: yfiF [C]

Synonym: HI0424

Alternate gene names: 16272372

Gene position: 447204-446149 (Counterclockwise)

Preceding gene: 16272374

Following gene: 16272370

Centisome position: 24.44

GC content: 39.87

Gene sequence:

>1056_bases
ATGAACGACAAACGCAAACCCTCTTTTCAATCCGCAGGCAAAACCTTTCAAGAACGCTCAGTGGGGGAAAAGTACAGAGA
AAAACCAACTCAAAATCGACCGCACTTTAATGATAAATTTAATGGAAATAGAAACGAAAAATCTCGTTTTCCTCGTGATA
AACAAGAAGTGAAAGAAACTAGAATTACGCAACTTTCTTTATCTCGTGCGCCATCAAATAAAAATGCAGAACAACCTAAG
GTTCAAGTGACAATAAAAAGTACGGGTACGGTTTATAAAACAAAAGAAAAGAAAACGGGTGCACTTTCTCCTCGTGCACC
TGAAAAAATTAAGAAAAACCGTGCGGAAGAAATGAAGGTTTACGGTGAAAATGCCTGTTTAGCTTTATTTGCAGAACGTC
CTGAAAGTATTGTTCGATTGTGGGCAACCGTGCAAATGTCACACAAAATTGGCGAGGTATTGAGTTATTTAGCCGAAAAC
AAGAAGGCTTATCACGTTGTAGATAGTGAAGAATTAGCTCGAGTGAGTGGAACAGAACATCACGGTGGCATTTGCTTATT
AGTGAAGAAACCGCGTGCTTTCACGTTGCAAGGATATTTGGATATTCCTCGTAACGAGGATTGTTTAGTGCTATTGGACA
ACGTGAATAATGCGCAAAATATTGGTGGCGTATTGCGTACTTGCGCTTATTTTGGCGTGAAAAATATTGTCGCCGATAAT
GTGGAAAATCTGTATTCGGCAGCCTCAATGCGCGTAGCAGAAGGTGGTGCAGAATATATTCGTGTGTTAGAAGCGGATTA
TATTGATTCAGCTTTAATGCAATTGCGTAAATCAGGCTATCAAATTATTCACGTATCGCACAATAAACAAGGCGACCCAT
TGGATAAAGTGCGGTTAAAAAATAAAGTGGTTTTTGTGCTAAGTGAAAGTTCTACGGAAAGTTTAGCGACGCCAGAAGAT
ACACAAGCGCGTTTAACGCTCGCAAGCCCAATTAAATCAGGCTTAAACATTGCAGTGAATGCGGGTGTGTTGTTAGCGAA
GTGGTATTTTAGATAG

Upstream 100 bases:

>100_bases
ACGTGCTTATTAATAGAAAAAAGGCTTTTTTTCGTTATAATGCTCACAAATTTTAGCATTTTTTGGCTAAAAAACAGCCA
TTTTTACTCAGGGAAACCCT

Downstream 100 bases:

>100_bases
TGTATGATTAAAGGGGAAAATGAATTTCCCCTTTAAAATTTCAAATAAAAGTCTTTAGTCAATTCAATAAACGCTTCCGT
GTATTGATTATCCGCATCAT

Product: rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MNDKRKPSFQSAGKTFQERSVGEKYREKPTQNRPHFNDKFNGNRNEKSRFPRDKQEVKETRITQLSLSRAPSNKNAEQPK
VQVTIKSTGTVYKTKEKKTGALSPRAPEKIKKNRAEEMKVYGENACLALFAERPESIVRLWATVQMSHKIGEVLSYLAEN
KKAYHVVDSEELARVSGTEHHGGICLLVKKPRAFTLQGYLDIPRNEDCLVLLDNVNNAQNIGGVLRTCAYFGVKNIVADN
VENLYSAASMRVAEGGAEYIRVLEADYIDSALMQLRKSGYQIIHVSHNKQGDPLDKVRLKNKVVFVLSESSTESLATPED
TQARLTLASPIKSGLNIAVNAGVLLAKWYFR

Sequences:

>Translated_351_residues
MNDKRKPSFQSAGKTFQERSVGEKYREKPTQNRPHFNDKFNGNRNEKSRFPRDKQEVKETRITQLSLSRAPSNKNAEQPK
VQVTIKSTGTVYKTKEKKTGALSPRAPEKIKKNRAEEMKVYGENACLALFAERPESIVRLWATVQMSHKIGEVLSYLAEN
KKAYHVVDSEELARVSGTEHHGGICLLVKKPRAFTLQGYLDIPRNEDCLVLLDNVNNAQNIGGVLRTCAYFGVKNIVADN
VENLYSAASMRVAEGGAEYIRVLEADYIDSALMQLRKSGYQIIHVSHNKQGDPLDKVRLKNKVVFVLSESSTESLATPED
TQARLTLASPIKSGLNIAVNAGVLLAKWYFR
>Mature_351_residues
MNDKRKPSFQSAGKTFQERSVGEKYREKPTQNRPHFNDKFNGNRNEKSRFPRDKQEVKETRITQLSLSRAPSNKNAEQPK
VQVTIKSTGTVYKTKEKKTGALSPRAPEKIKKNRAEEMKVYGENACLALFAERPESIVRLWATVQMSHKIGEVLSYLAEN
KKAYHVVDSEELARVSGTEHHGGICLLVKKPRAFTLQGYLDIPRNEDCLVLLDNVNNAQNIGGVLRTCAYFGVKNIVADN
VENLYSAASMRVAEGGAEYIRVLEADYIDSALMQLRKSGYQIIHVSHNKQGDPLDKVRLKNKVVFVLSESSTESLATPED
TQARLTLASPIKSGLNIAVNAGVLLAKWYFR

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family

Homologues:

Organism=Escherichia coli, GI1788935, Length=312, Percent_Identity=38.1410256410256, Blast_Score=223, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y424_HAEIN (P44703)

Other databases:

- EMBL:   L42023
- PIR:   D64152
- RefSeq:   NP_438585.1
- ProteinModelPortal:   P44703
- GeneID:   949529
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0424
- NMPDR:   fig|71421.1.peg.398
- TIGR:   HI_0424
- HOGENOM:   HBG714057
- OMA:   IVRLWAT
- ProtClustDB:   CLSK2518358
- BioCyc:   HINF71421:HI_0424-MONOMER
- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479
- PIRSF:   PIRSF006280
- SMART:   SM00967

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind

EC number: 2.1.1.- [C]

Molecular weight: Translated: 39397; Mature: 39397

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKRKPSFQSAGKTFQERSVGEKYREKPTQNRPHFNDKFNGNRNEKSRFPRDKQEVKET
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHH
RITQLSLSRAPSNKNAEQPKVQVTIKSTGTVYKTKEKKTGALSPRAPEKIKKNRAEEMKV
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHE
YGENACLALFAERPESIVRLWATVQMSHKIGEVLSYLAENKKAYHVVDSEELARVSGTEH
ECCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCC
HGGICLLVKKPRAFTLQGYLDIPRNEDCLVLLDNVNNAQNIGGVLRTCAYFGVKNIVADN
CCCEEEEEECCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
VENLYSAASMRVAEGGAEYIRVLEADYIDSALMQLRKSGYQIIHVSHNKQGDPLDKVRLK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHC
NKVVFVLSESSTESLATPEDTQARLTLASPIKSGLNIAVNAGVLLAKWYFR
CEEEEEEECCCCCCCCCCCCCCEEEEEHHHHHCCCEEEEECCEEEEEECCC
>Mature Secondary Structure
MNDKRKPSFQSAGKTFQERSVGEKYREKPTQNRPHFNDKFNGNRNEKSRFPRDKQEVKET
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHH
RITQLSLSRAPSNKNAEQPKVQVTIKSTGTVYKTKEKKTGALSPRAPEKIKKNRAEEMKV
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHE
YGENACLALFAERPESIVRLWATVQMSHKIGEVLSYLAENKKAYHVVDSEELARVSGTEH
ECCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCC
HGGICLLVKKPRAFTLQGYLDIPRNEDCLVLLDNVNNAQNIGGVLRTCAYFGVKNIVADN
CCCEEEEEECCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
VENLYSAASMRVAEGGAEYIRVLEADYIDSALMQLRKSGYQIIHVSHNKQGDPLDKVRLK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHC
NKVVFVLSESSTESLATPEDTQARLTLASPIKSGLNIAVNAGVLLAKWYFR
CEEEEEEECCCCCCCCCCCCCCEEEEEHHHHHCCCEEEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800