Click here to switch to the map view.

The map label for this gene is yfiC [C]

Identifier: 16272371

GI number: 16272371

Start: 445394

End: 446116

Strand: Direct

Name: yfiC [C]

Synonym: HI0423

Alternate gene names: 16272371

Gene position: 445394-446116 (Clockwise)

Preceding gene: 16272369

Following gene: 16272373

Centisome position: 24.34

GC content: 36.38

Gene sequence:

>723_bases
GTGCGGTCAATTTACGAAAAATTTATGAGCAGATTTACCTTTAAACAGTTCCATATCAATCAAAATTCTTGTGCAATGAA
AGTCGGCACTGATGGCATTTTGTTAGGTGCTTGGGCTGATGTAAAGCATTGTAAAAATATTTTAGATATGGGCTGTGGCA
CAGGATTATTGGCGTTAATGTTAGCACAACGAACTGAAGAAAACTGTCAAATTCAAGCGGTTGAGTTGGATCCCATTGCA
GCAAAACAAGCACAAGAAAACATCAATAATTCAGTGTGGAAAAATCGCATTCAACTTACTCAAGTGGATATACAACATTT
TTTACAAACCACTGAACAAACCTTTGATTTAATTGTGGCAAATCCACCTTATTTTGAACAAGGTATTGCTTGTAAAAATG
AAGAACGAGAATTGGCTCGTTATACCAAGCAAAGCCACTTAAATTGGTTAGAATGGGCTGCAACACGCTTGTCGGAAAAT
GGGAGAATAAGTTTTGTTTTGCCTTATGAGGCAGGCAAAACGCTCACAAAATCCACCGCACTTTTTTGCATAAAACAGAC
GAATGTCATCACTAAAATAGGAAAAACGCCCCAGAGAATGTTGCTTACCTTTGCCAAACAACCCGAAGTTTTAATGCAAG
ATCAATTGGTAATTTATGATGCGGATAATCAATACACGGAAGCGTTTATTGAATTGACTAAAGACTTTTATTTGAAATTT
TAA

Upstream 100 bases:

>100_bases
GTGCCTTTAAAAGCTCAGGAGAAAGATCGAATTGTTCAAATTGGGATAAATTCATTATTGTGCGGTTCTTTGTTAAAATT
GTCGAGGATTATACCTGAAA

Downstream 100 bases:

>100_bases
AGGGGAAATTCATTTTCCCCTTTAATCATACACTATCTAAAATACCACTTCGCTAACAACACACCCGCATTCACTGCAAT
GTTTAAGCCTGATTTAATTG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA m6A37 methyltransferase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRSIYEKFMSRFTFKQFHINQNSCAMKVGTDGILLGAWADVKHCKNILDMGCGTGLLALMLAQRTEENCQIQAVELDPIA
AKQAQENINNSVWKNRIQLTQVDIQHFLQTTEQTFDLIVANPPYFEQGIACKNEERELARYTKQSHLNWLEWAATRLSEN
GRISFVLPYEAGKTLTKSTALFCIKQTNVITKIGKTPQRMLLTFAKQPEVLMQDQLVIYDADNQYTEAFIELTKDFYLKF

Sequences:

>Translated_240_residues
MRSIYEKFMSRFTFKQFHINQNSCAMKVGTDGILLGAWADVKHCKNILDMGCGTGLLALMLAQRTEENCQIQAVELDPIA
AKQAQENINNSVWKNRIQLTQVDIQHFLQTTEQTFDLIVANPPYFEQGIACKNEERELARYTKQSHLNWLEWAATRLSEN
GRISFVLPYEAGKTLTKSTALFCIKQTNVITKIGKTPQRMLLTFAKQPEVLMQDQLVIYDADNQYTEAFIELTKDFYLKF
>Mature_240_residues
MRSIYEKFMSRFTFKQFHINQNSCAMKVGTDGILLGAWADVKHCKNILDMGCGTGLLALMLAQRTEENCQIQAVELDPIA
AKQAQENINNSVWKNRIQLTQVDIQHFLQTTEQTFDLIVANPPYFEQGIACKNEERELARYTKQSHLNWLEWAATRLSEN
GRISFVLPYEAGKTLTKSTALFCIKQTNVITKIGKTPQRMLLTFAKQPEVLMQDQLVIYDADNQYTEAFIELTKDFYLKF

Specific function: Specifically methylates the adenine in position 37 of tRNA(Val) [H]

COG id: COG4123

COG function: function code R; Predicted O-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. tRNA (adenine-N(6)-)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082131, Length=231, Percent_Identity=47.6190476190476, Blast_Score=208, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR020596
- InterPro:   IPR007848
- InterPro:   IPR022882 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: =2.1.1.55 [H]

Molecular weight: Translated: 27618; Mature: 27618

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIYEKFMSRFTFKQFHINQNSCAMKVGTDGILLGAWADVKHCKNILDMGCGTGLLALM
CHHHHHHHHHHHHHHHEEECCCCEEEEECCCCEEEECCHHHHHHHHHHHCCCCHHHHHHH
LAQRTEENCQIQAVELDPIAAKQAQENINNSVWKNRIQLTQVDIQHFLQTTEQTFDLIVA
HHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
NPPYFEQGIACKNEERELARYTKQSHLNWLEWAATRLSENGRISFVLPYEAGKTLTKSTA
CCCCHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHE
LFCIKQTNVITKIGKTPQRMLLTFAKQPEVLMQDQLVIYDADNQYTEAFIELTKDFYLKF
EEEEECCCHHHHHCCCHHHHHHHHCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSIYEKFMSRFTFKQFHINQNSCAMKVGTDGILLGAWADVKHCKNILDMGCGTGLLALM
CHHHHHHHHHHHHHHHEEECCCCEEEEECCCCEEEECCHHHHHHHHHHHCCCCHHHHHHH
LAQRTEENCQIQAVELDPIAAKQAQENINNSVWKNRIQLTQVDIQHFLQTTEQTFDLIVA
HHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
NPPYFEQGIACKNEERELARYTKQSHLNWLEWAATRLSENGRISFVLPYEAGKTLTKSTA
CCCCHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHE
LFCIKQTNVITKIGKTPQRMLLTFAKQPEVLMQDQLVIYDADNQYTEAFIELTKDFYLKF
EEEEECCCHHHHHCCCHHHHHHHHCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA