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The map label for this gene is mesJ [H]

Identifier: 16272353

GI number: 16272353

Start: 424804

End: 426096

Strand: Reverse

Name: mesJ [H]

Synonym: HI0404

Alternate gene names: 16272353

Gene position: 426096-424804 (Counterclockwise)

Preceding gene: 16272354

Following gene: 16272349

Centisome position: 23.28

GC content: 37.97

Gene sequence:

>1293_bases
ATGGATTTACTTTCAGACATTGAAAAACAACTCCAAAAAGCCACCGCTCAAGCATTTCTTATTGCCTTGAGTGGTGGTTT
AGATTCCACTGTACTTCTTTCTTTATTTGCAAAACTTTGTCAAAAACAACCGCACTTACCGCCTTTATCAATACGAGCAA
TCCATATTCATCACGGATTAAGCCCCAATGCCGACAGTTGGGCTAAACATTGTCAAGACTTATGCGATCAATTTCAAATT
CCATTGATTATTGAGCGAGTACAAGTAGATAAAACTAATGGTATTGAGGCTGGCGCACGTGAAGCACGCTATCAAGCCAT
CAAAAAATATCTTCAAACACAAGAAATGCTGGTCACAGCGCATCATCTAAACGATCAAACTGAAACCTTTTTCTTAGCCC
TAAAACGTGGCAGTGGGCTAAAAGGCTTAGGCGCAATGCAACAACAAAGTGTATTATTCGGCATGCCAATTTTACGCCCT
TTGTTAGGCTTTACTCGCACCCAGTTAGAAAATTACGCGCAAAAAGAAAAACTCAACTGGATTACAGACGAAAGTAATGA
AGATAATCGATACGATCGTAATTTCTTACGCAATGAAATTTTGCCAGAATTACGTGAACGCTGGGCTCATTTTGATTTAG
CCGTGCAACGTTCAGCACAACATTGTTTTGAGCAACAACAGCTAATTAATGATTTATTGAGCGAAATTTTTACAGAACAT
TGTCAAATTAAAAACCAATTTAAACTGTGCCAGTTCCGCCAATATTCTCTCGCAAAACAGACCGCACTTTTGCGTATGTG
GCTTGCCGAAAACCAGCTTGAAATGCCAAGTAAACGCCAGCTTACACAGCTTATTAATGATGTCATTTTTGCAAAAGAAG
AGGCAAATCCTCAATTTCAGCTTGTAAATAAAGTTATTCGACGTTATCAAGATAGTTTATATTTAACTAAGCCGTTTTCT
GATCTAACCAAATGCACCCTAAAATTAGAACAAAATACGCTAAATTTACCCGATGATTTAGGCAATCTCACAGTTCAAGA
AAATGAACATAATCTGATTTTCTACTGGCAGGATTACTCTGTTACGCTGGAAAAAACGAACTTGCCTATTTCTATTCGTT
TTGGCTATTCGGGCAAAGTGAAACATTATCCGAAACGCCCAAGAGAAGATATAAAAAAAATCTGGCAAGAATTAGGCGTG
CCACCTTGGGAACGAAATCGAATCCCCTTAATTTTTTATGGCAACGAGCTAAAAAGTGCGGTCGGTTTTTTTCGTGTTTT
AAAATCAAGTTAA

Upstream 100 bases:

>100_bases
AAACCACCTTTGAACTGGGTTCTTACGAATTACAAACGATTGCTGCACGTTTTGAAATTCTTAATCCAAGCAGCAATTAT
AAAGCTGAAAAAGTTGCCTA

Downstream 100 bases:

>100_bases
AGCGACTTTGTTTCTAAAAATGCGTTCAGAATTTGAGCGGTGAATTCTTTTCTTAGATAAAAACCTAAAGGCAAGGTATC
TATAATTTCGCCATTTTTTC

Product: cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MDLLSDIEKQLQKATAQAFLIALSGGLDSTVLLSLFAKLCQKQPHLPPLSIRAIHIHHGLSPNADSWAKHCQDLCDQFQI
PLIIERVQVDKTNGIEAGAREARYQAIKKYLQTQEMLVTAHHLNDQTETFFLALKRGSGLKGLGAMQQQSVLFGMPILRP
LLGFTRTQLENYAQKEKLNWITDESNEDNRYDRNFLRNEILPELRERWAHFDLAVQRSAQHCFEQQQLINDLLSEIFTEH
CQIKNQFKLCQFRQYSLAKQTALLRMWLAENQLEMPSKRQLTQLINDVIFAKEEANPQFQLVNKVIRRYQDSLYLTKPFS
DLTKCTLKLEQNTLNLPDDLGNLTVQENEHNLIFYWQDYSVTLEKTNLPISIRFGYSGKVKHYPKRPREDIKKIWQELGV
PPWERNRIPLIFYGNELKSAVGFFRVLKSS

Sequences:

>Translated_430_residues
MDLLSDIEKQLQKATAQAFLIALSGGLDSTVLLSLFAKLCQKQPHLPPLSIRAIHIHHGLSPNADSWAKHCQDLCDQFQI
PLIIERVQVDKTNGIEAGAREARYQAIKKYLQTQEMLVTAHHLNDQTETFFLALKRGSGLKGLGAMQQQSVLFGMPILRP
LLGFTRTQLENYAQKEKLNWITDESNEDNRYDRNFLRNEILPELRERWAHFDLAVQRSAQHCFEQQQLINDLLSEIFTEH
CQIKNQFKLCQFRQYSLAKQTALLRMWLAENQLEMPSKRQLTQLINDVIFAKEEANPQFQLVNKVIRRYQDSLYLTKPFS
DLTKCTLKLEQNTLNLPDDLGNLTVQENEHNLIFYWQDYSVTLEKTNLPISIRFGYSGKVKHYPKRPREDIKKIWQELGV
PPWERNRIPLIFYGNELKSAVGFFRVLKSS
>Mature_430_residues
MDLLSDIEKQLQKATAQAFLIALSGGLDSTVLLSLFAKLCQKQPHLPPLSIRAIHIHHGLSPNADSWAKHCQDLCDQFQI
PLIIERVQVDKTNGIEAGAREARYQAIKKYLQTQEMLVTAHHLNDQTETFFLALKRGSGLKGLGAMQQQSVLFGMPILRP
LLGFTRTQLENYAQKEKLNWITDESNEDNRYDRNFLRNEILPELRERWAHFDLAVQRSAQHCFEQQQLINDLLSEIFTEH
CQIKNQFKLCQFRQYSLAKQTALLRMWLAENQLEMPSKRQLTQLINDVIFAKEEANPQFQLVNKVIRRYQDSLYLTKPFS
DLTKCTLKLEQNTLNLPDDLGNLTVQENEHNLIFYWQDYSVTLEKTNLPISIRFGYSGKVKHYPKRPREDIKKIWQELGV
PPWERNRIPLIFYGNELKSAVGFFRVLKSS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=414, Percent_Identity=42.7536231884058, Blast_Score=319, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 50053; Mature: 50053

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLSDIEKQLQKATAQAFLIALSGGLDSTVLLSLFAKLCQKQPHLPPLSIRAIHIHHGL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCC
SPNADSWAKHCQDLCDQFQIPLIIERVQVDKTNGIEAGAREARYQAIKKYLQTQEMLVTA
CCCHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HHLNDQTETFFLALKRGSGLKGLGAMQQQSVLFGMPILRPLLGFTRTQLENYAQKEKLNW
HHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
ITDESNEDNRYDRNFLRNEILPELRERWAHFDLAVQRSAQHCFEQQQLINDLLSEIFTEH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CQIKNQFKLCQFRQYSLAKQTALLRMWLAENQLEMPSKRQLTQLINDVIFAKEEANPQFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
LVNKVIRRYQDSLYLTKPFSDLTKCTLKLEQNTLNLPDDLGNLTVQENEHNLIFYWQDYS
HHHHHHHHHHHCEEEECCHHHHHHHHHEECCCCCCCCHHHCCCEEEECCCCEEEEEECCE
VTLEKTNLPISIRFGYSGKVKHYPKRPREDIKKIWQELGVPPWERNRIPLIFYGNELKSA
EEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHH
VGFFRVLKSS
HHHHHHHHCC
>Mature Secondary Structure
MDLLSDIEKQLQKATAQAFLIALSGGLDSTVLLSLFAKLCQKQPHLPPLSIRAIHIHHGL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCC
SPNADSWAKHCQDLCDQFQIPLIIERVQVDKTNGIEAGAREARYQAIKKYLQTQEMLVTA
CCCHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HHLNDQTETFFLALKRGSGLKGLGAMQQQSVLFGMPILRPLLGFTRTQLENYAQKEKLNW
HHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
ITDESNEDNRYDRNFLRNEILPELRERWAHFDLAVQRSAQHCFEQQQLINDLLSEIFTEH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CQIKNQFKLCQFRQYSLAKQTALLRMWLAENQLEMPSKRQLTQLINDVIFAKEEANPQFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
LVNKVIRRYQDSLYLTKPFSDLTKCTLKLEQNTLNLPDDLGNLTVQENEHNLIFYWQDYS
HHHHHHHHHHHCEEEECCHHHHHHHHHEECCCCCCCCHHHCCCEEEECCCCEEEEEECCE
VTLEKTNLPISIRFGYSGKVKHYPKRPREDIKKIWQELGVPPWERNRIPLIFYGNELKSA
EEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHH
VGFFRVLKSS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA