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The map label for this gene is ampD

Identifier: 16272255

GI number: 16272255

Start: 334212

End: 334766

Strand: Direct

Name: ampD

Synonym: HI0300

Alternate gene names: 16272255

Gene position: 334212-334766 (Clockwise)

Preceding gene: 16272250

Following gene: 16272256

Centisome position: 18.26

GC content: 37.48

Gene sequence:

>555_bases
ATGCGAAAAATAAAAGATATTGAGAAAGGATTGCTGACCGATTGTCGGCAAATTCAGTCACCTCACTTTGATAAACGCCC
AAATCCGCAGGATATTTCCTTATTAGTTATTCATTATATTAGTCTACCGCCAGAACAATTTGGCGGCGGATATGTGGATG
ACTTTTTTCAAGGGAAATTAGATCCTAAGATTCATCCTTATTTTGCAGAAATTTATCAAATGCGTGTTTCAGCACATTGT
TTGATCGAACGAAATGGGCGAATTACGCAATATGTCAATTTTAATGATCGCGCTTGGCACGCAGGCGTATCGAATTTTCA
AGGACGAGAAAAATGCAATGATTTTGCCATTGGAATTGAGTTGGAGGGGAGTAATGAACAGCCTTTTACCGACGCTCAAT
ATTTTTCATTGCAAGAACTTACCAATGTAATAATGAAAAGTTACCCGAAGATTACTAAAGATCGAATTGTTGGACATTGC
GATATTTCGCCGAAACGTAAGATCGATCCTGGTCAATATTTTGATTGGGAACGTTATTTATCCAGTGTGAAATAA

Upstream 100 bases:

>100_bases
TAAGCCTTTGTTGGCAACGACATTGTGAAAAGGAAAAATTTGTTTGTAAAATTGACCGCACTTTTTCTGCGATCCTGATC
GCAAAAAAAAGGAAAAATGT

Downstream 100 bases:

>100_bases
TCTGTATAAAAATTAAACATCAAATTTTGGGTATTCTGTGTATTCTAAATGCGTTCATTCTTTTACTTCTAACCAGTTGA
ATTAAAAGGAATAAATTTAA

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MRKIKDIEKGLLTDCRQIQSPHFDKRPNPQDISLLVIHYISLPPEQFGGGYVDDFFQGKLDPKIHPYFAEIYQMRVSAHC
LIERNGRITQYVNFNDRAWHAGVSNFQGREKCNDFAIGIELEGSNEQPFTDAQYFSLQELTNVIMKSYPKITKDRIVGHC
DISPKRKIDPGQYFDWERYLSSVK

Sequences:

>Translated_184_residues
MRKIKDIEKGLLTDCRQIQSPHFDKRPNPQDISLLVIHYISLPPEQFGGGYVDDFFQGKLDPKIHPYFAEIYQMRVSAHC
LIERNGRITQYVNFNDRAWHAGVSNFQGREKCNDFAIGIELEGSNEQPFTDAQYFSLQELTNVIMKSYPKITKDRIVGHC
DISPKRKIDPGQYFDWERYLSSVK
>Mature_184_residues
MRKIKDIEKGLLTDCRQIQSPHFDKRPNPQDISLLVIHYISLPPEQFGGGYVDDFFQGKLDPKIHPYFAEIYQMRVSAHC
LIERNGRITQYVNFNDRAWHAGVSNFQGREKCNDFAIGIELEGSNEQPFTDAQYFSLQELTNVIMKSYPKITKDRIVGHC
DISPKRKIDPGQYFDWERYLSSVK

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family

Homologues:

Organism=Escherichia coli, GI1786300, Length=173, Percent_Identity=56.0693641618497, Blast_Score=220, Evalue=4e-59,
Organism=Escherichia coli, GI1787092, Length=163, Percent_Identity=34.9693251533742, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMPD_HAEIN (P44624)

Other databases:

- EMBL:   L42023
- PIR:   F64060
- RefSeq:   NP_438467.1
- ProteinModelPortal:   P44624
- SMR:   P44624
- GeneID:   949420
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0300
- NMPDR:   fig|71421.1.peg.282
- TIGR:   HI_0300
- HOGENOM:   HBG700567
- OMA:   LFQGCLD
- ProtClustDB:   PRK11789
- BioCyc:   HINF71421:HI_0300-MONOMER
- GO:   GO:0005737
- InterPro:   IPR002502
- Gene3D:   G3DSA:3.40.80.10
- SMART:   SM00644

Pfam domain/function: PF01510 Amidase_2; SSF55846 Amidase_2

EC number: =3.5.1.28

Molecular weight: Translated: 21449; Mature: 21449

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIKDIEKGLLTDCRQIQSPHFDKRPNPQDISLLVIHYISLPPEQFGGGYVDDFFQGKL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEHHCCCHHHHCCCCHHHHHCCCC
DPKIHPYFAEIYQMRVSAHCLIERNGRITQYVNFNDRAWHAGVSNFQGREKCNDFAIGIE
CCCCCHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCHHHCCCCCCCCHHCCCCEEEEEE
LEGSNEQPFTDAQYFSLQELTNVIMKSYPKITKDRIVGHCDISPKRKIDPGQYFDWERYL
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCCCCHHHHH
SSVK
HHCC
>Mature Secondary Structure
MRKIKDIEKGLLTDCRQIQSPHFDKRPNPQDISLLVIHYISLPPEQFGGGYVDDFFQGKL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEHHCCCHHHHCCCCHHHHHCCCC
DPKIHPYFAEIYQMRVSAHCLIERNGRITQYVNFNDRAWHAGVSNFQGREKCNDFAIGIE
CCCCCHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCHHHCCCCCCCCHHCCCCEEEEEE
LEGSNEQPFTDAQYFSLQELTNVIMKSYPKITKDRIVGHCDISPKRKIDPGQYFDWERYL
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCCCCHHHHH
SSVK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800