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The map label for this gene is rpoH

Identifier: 16272227

GI number: 16272227

Start: 302991

End: 303836

Strand: Direct

Name: rpoH

Synonym: HI0269

Alternate gene names: 16272227

Gene position: 302991-303836 (Clockwise)

Preceding gene: 16272226

Following gene: 16272233

Centisome position: 16.56

GC content: 40.54

Gene sequence:

>846_bases
ATGGATAAAGAAACACAAATGATGTTAGTTCCTCAAGGCAGTATCGAAGGTTATATTCGTGCAGCAAATGAATATCCGAT
GCTGACCGCAGAGGAAGAAAAGGAATTGGCAGAACGTTTGTATTATCACGAGGATTTAGATGCAGCGAAAAAACTCATTT
TGTCGCATCTTCGTTTCGTGATCCACGTGGCGCGTGGCTATTCTGGCTATGGTTTGCCACAAGCTGACTTAATCCAAGAG
GGCAATATCGGTTTAATGAAAGCGGTCAAACGTTTTAACCCTGAAGTTGGTGTACGCTTGGTGTCATTCGCTGTGCATTG
GATTAAAGCTGAGATCCACGAATATGTTCTTCGTAACTGGCGTATCGTAAAAGTCGCAACCACGAAAGCTCAACGTAAAT
TATTTTTCAACTTACGTAAAACTAAACAACGTTTAGGTTGGTTCAATGAAAATGAAGTTGATATGGTCGCAAATGAATTG
GGTGTTTCCAAAGAAGATGTGATCGAAATGGAATCTCGAATGAGCGGTGCGGATGTCGGTTTTGATTTACCCACTGATGA
TGCAGAAACAGAGACCTATTCTCCAGCCCTTTATTTGGAAGATAAAAGTTCTAATTTCGCAGCAGAGCTGGAAAATGAAA
ACTTTGAAAGCCAAGCAACCGAGCAATTAGGTGCGGCGTTACAAAGTCTAGATGCACGTAGTCAGGATATTATCAAAGCT
CGTTGGTTAGATGATAACAAAGCCACATTGCACGACTTGGCAGCAAAATATAATGTCTCTGCGGAACGTATTCGTCAATT
AGAAACTAATGCACTGAAAAAATTAAAAAGTGCGGTGAATTTTTAA

Upstream 100 bases:

>100_bases
GATAAAATCTGTCTTATTAACAAATTGGTTCAATAAGATAGCTTATTGTGCTATAACTAGGTACATTGATTGGCAAAAGC
CAAAATAAAGAAGGGAAACG

Downstream 100 bases:

>100_bases
ATAATTTAGAAAAAGAAAACCTGCTGATTAGCAGGTTTTTTATTTCTCATTTAACCGTAATTTCAAATCTTCAGCAGTTT
GGCAAGTCTTAATCTTATCA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MDKETQMMLVPQGSIEGYIRAANEYPMLTAEEEKELAERLYYHEDLDAAKKLILSHLRFVIHVARGYSGYGLPQADLIQE
GNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVDMVANEL
GVSKEDVIEMESRMSGADVGFDLPTDDAETETYSPALYLEDKSSNFAAELENENFESQATEQLGAALQSLDARSQDIIKA
RWLDDNKATLHDLAAKYNVSAERIRQLETNALKKLKSAVNF

Sequences:

>Translated_281_residues
MDKETQMMLVPQGSIEGYIRAANEYPMLTAEEEKELAERLYYHEDLDAAKKLILSHLRFVIHVARGYSGYGLPQADLIQE
GNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVDMVANEL
GVSKEDVIEMESRMSGADVGFDLPTDDAETETYSPALYLEDKSSNFAAELENENFESQATEQLGAALQSLDARSQDIIKA
RWLDDNKATLHDLAAKYNVSAERIRQLETNALKKLKSAVNF
>Mature_281_residues
MDKETQMMLVPQGSIEGYIRAANEYPMLTAEEEKELAERLYYHEDLDAAKKLILSHLRFVIHVARGYSGYGLPQADLIQE
GNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVDMVANEL
GVSKEDVIEMESRMSGADVGFDLPTDDAETETYSPALYLEDKSSNFAAELENENFESQATEQLGAALQSLDARSQDIIKA
RWLDDNKATLHDLAAKYNVSAERIRQLETNALKKLKSAVNF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily

Homologues:

Organism=Escherichia coli, GI1789871, Length=280, Percent_Identity=71.0714285714286, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1789098, Length=280, Percent_Identity=27.8571428571429, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789448, Length=257, Percent_Identity=29.5719844357977, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): RP32_HAEIN (P44404)

Other databases:

- EMBL:   L42023
- PIR:   H64058
- RefSeq:   NP_438438.1
- ProteinModelPortal:   P44404
- SMR:   P44404
- GeneID:   949393
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0269
- TIGR:   HI_0269
- HOGENOM:   HBG745096
- OMA:   VNSIPVL
- ProtClustDB:   PRK06596
- BioCyc:   HINF71421:HI_0269-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02392
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 32034; Mature: 32034

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKETQMMLVPQGSIEGYIRAANEYPMLTAEEEKELAERLYYHEDLDAAKKLILSHLRFV
CCCCCCEEEEECCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IHVARGYSGYGLPQADLIQEGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNW
HHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVDMVANELGVSKEDVIEMESRMSGADVG
EEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
FDLPTDDAETETYSPALYLEDKSSNFAAELENENFESQATEQLGAALQSLDARSQDIIKA
CCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHE
RWLDDNKATLHDLAAKYNVSAERIRQLETNALKKLKSAVNF
EECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKETQMMLVPQGSIEGYIRAANEYPMLTAEEEKELAERLYYHEDLDAAKKLILSHLRFV
CCCCCCEEEEECCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IHVARGYSGYGLPQADLIQEGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNW
HHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVDMVANELGVSKEDVIEMESRMSGADVG
EEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
FDLPTDDAETETYSPALYLEDKSSNFAAELENENFESQATEQLGAALQSLDARSQDIIKA
CCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHE
RWLDDNKATLHDLAAKYNVSAERIRQLETNALKKLKSAVNF
EECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800