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The map label for this gene is yaiM [C]

Identifier: 16272149

GI number: 16272149

Start: 198638

End: 199465

Strand: Reverse

Name: yaiM [C]

Synonym: HI0184

Alternate gene names: 16272149

Gene position: 199465-198638 (Counterclockwise)

Preceding gene: 16272150

Following gene: 16272146

Centisome position: 10.9

GC content: 43.84

Gene sequence:

>828_bases
ATGAAACTAATTGAACAACATCAAATTTTTGGCGGTTCGCAGCAAGTTTGGGCGCATAATGCTCAAACACTTCAATGTGA
AATGAAATTTGCCGTTTATTTGCCAAATAATCCAGAAAATCGACCGCTTGGTGTGATTTATTGGCTTTCAGGCTTAACTT
GTACTGAGCAAAATTTCATTACCAAATCAGGCTTCCAGCGTTACGCGGCAGAACATCAAGTGATTGTTGTTGCTCCCGAT
ACAAGCCCTCGTGGAGAGCAAGTGCCGAATGATGCGGCTTACGATTTAGGGCAGGGAGCGGGCTTTTATCTTAATGCGAC
CGAGCAGCCTTGGGCGACGAATTATCAAATGTATGATTATATCTTGAATGAATTGCCTGATTTGATTGAAGCAAATTTCC
CTACCAACGGCAAACGTTCCATTATGGGACATTCAATGGGTGGACACGGTGCATTGGTATTGGCACTGCGAAATCGGGAA
CGTTATCAAAGCGTTTCTGCCTTTTCGCCCATTTTGTCGCCAAGCCTTGTGCCTTGGGGAGAAAAAGCCTTTTCTGCCTA
TTTAGGGGAAGATCGTGAAAAATGGCAGCAATATGATGCCAGCTCGCTCATTCAACAAGGCTATAAAGTGCAAGGTATGC
GCATTGATCAGGGCTTGGAAGATGAGTTTTTACCGACACAATTGCGTACCGAAGATTTTATAGAAACCTGTCGAGTGGCA
AATCAGCCAGTCGATGTGCGCTTCCATAAAGGCTATGATCACAGCTATTACTTCATCGCCAGTTTTATTGGCGAGCATAT
TGCCTATCATGCGGAATTTTTGAAGTAA

Upstream 100 bases:

>100_bases
TTGTGACTCACACAATGCCACTTGATAAAATTAATGAAGCCTTTGAATTAATGCACGAAGGTAAATCAATCCGTACTGTT
ATTCACTACTAAGGTACGCT

Downstream 100 bases:

>100_bases
GCTAAAGAGCGGTCAGTTTTCAAAGCAGTTTGGGATAGCCGGCACGAGGGCGGTAAAAAGTGCCGGCAATAGTTTATTCA
GTCTGAACTAGAAGAACCAA

Product: esterase

Products: NA

Alternate protein names: FGH

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLGVIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPD
TSPRGEQVPNDAAYDLGQGAGFYLNATEQPWATNYQMYDYILNELPDLIEANFPTNGKRSIMGHSMGGHGALVLALRNRE
RYQSVSAFSPILSPSLVPWGEKAFSAYLGEDREKWQQYDASSLIQQGYKVQGMRIDQGLEDEFLPTQLRTEDFIETCRVA
NQPVDVRFHKGYDHSYYFIASFIGEHIAYHAEFLK

Sequences:

>Translated_275_residues
MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLGVIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPD
TSPRGEQVPNDAAYDLGQGAGFYLNATEQPWATNYQMYDYILNELPDLIEANFPTNGKRSIMGHSMGGHGALVLALRNRE
RYQSVSAFSPILSPSLVPWGEKAFSAYLGEDREKWQQYDASSLIQQGYKVQGMRIDQGLEDEFLPTQLRTEDFIETCRVA
NQPVDVRFHKGYDHSYYFIASFIGEHIAYHAEFLK
>Mature_275_residues
MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLGVIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPD
TSPRGEQVPNDAAYDLGQGAGFYLNATEQPWATNYQMYDYILNELPDLIEANFPTNGKRSIMGHSMGGHGALVLALRNRE
RYQSVSAFSPILSPSLVPWGEKAFSAYLGEDREKWQQYDASSLIQQGYKVQGMRIDQGLEDEFLPTQLRTEDFIETCRVA
NQPVDVRFHKGYDHSYYFIASFIGEHIAYHAEFLK

Specific function: Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate

COG id: COG0627

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the esterase D family

Homologues:

Organism=Homo sapiens, GI33413400, Length=278, Percent_Identity=54.6762589928058, Blast_Score=317, Evalue=6e-87,
Organism=Escherichia coli, GI1786551, Length=274, Percent_Identity=62.4087591240876, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1788477, Length=276, Percent_Identity=52.536231884058, Blast_Score=309, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17510185, Length=278, Percent_Identity=47.841726618705, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6322393, Length=296, Percent_Identity=40.8783783783784, Blast_Score=227, Evalue=1e-60,
Organism=Drosophila melanogaster, GI45551932, Length=281, Percent_Identity=49.4661921708185, Blast_Score=278, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24648347, Length=281, Percent_Identity=49.4661921708185, Blast_Score=277, Evalue=5e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SFGH_HAEIN (P44556)

Other databases:

- EMBL:   L42023
- PIR:   A64145
- RefSeq:   NP_438352.1
- ProteinModelPortal:   P44556
- SMR:   P44556
- GeneID:   951093
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0184
- NMPDR:   fig|71421.1.peg.173
- TIGR:   HI_0184
- HOGENOM:   HBG286713
- OMA:   KRSIMGH
- ProtClustDB:   CLSK877992
- BioCyc:   HINF71421:HI_0184-MONOMER
- InterPro:   IPR000801
- InterPro:   IPR014186
- PANTHER:   PTHR10061
- TIGRFAMs:   TIGR02821

Pfam domain/function: PF00756 Esterase

EC number: =3.1.2.12

Molecular weight: Translated: 31317; Mature: 31317

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: ACT_SITE 145-145 ACT_SITE 221-221 ACT_SITE 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLGVIYWLSGLTCTEQNFI
CCCCHHHHHCCCCCCEEECCCEEEEEEEEEEEECCCCCCCCCEEEEEEECCCEECCCCHH
TKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDAAYDLGQGAGFYLNATEQPWATNYQMYDY
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCCHHHHHH
ILNELPDLIEANFPTNGKRSIMGHSMGGHGALVLALRNRERYQSVSAFSPILSPSLVPWG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCC
EKAFSAYLGEDREKWQQYDASSLIQQGYKVQGMRIDQGLEDEFLPTQLRTEDFIETCRVA
HHHHHHHHCCCHHHHHHCCHHHHHHCCCEEECEEECCCCCCCCCCCCCCHHHHHHHHHHC
NQPVDVRFHKGYDHSYYFIASFIGEHIAYHAEFLK
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLGVIYWLSGLTCTEQNFI
CCCCHHHHHCCCCCCEEECCCEEEEEEEEEEEECCCCCCCCCEEEEEEECCCEECCCCHH
TKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDAAYDLGQGAGFYLNATEQPWATNYQMYDY
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCCHHHHHH
ILNELPDLIEANFPTNGKRSIMGHSMGGHGALVLALRNRERYQSVSAFSPILSPSLVPWG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCC
EKAFSAYLGEDREKWQQYDASSLIQQGYKVQGMRIDQGLEDEFLPTQLRTEDFIETCRVA
HHHHHHHHCCCHHHHHHCCHHHHHHCCCEEECEEECCCCCCCCCCCCCCHHHHHHHHHHC
NQPVDVRFHKGYDHSYYFIASFIGEHIAYHAEFLK
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023