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The map label for this gene is dnaQ

Identifier: 16272104

GI number: 16272104

Start: 152492

End: 153262

Strand: Reverse

Name: dnaQ

Synonym: HI0137

Alternate gene names: 16272104

Gene position: 153262-152492 (Counterclockwise)

Preceding gene: 16272107

Following gene: 16272103

Centisome position: 8.37

GC content: 36.54

Gene sequence:

>771_bases
ATGATTAATCCCAATCGCCAAATTGTATTAGATACTGAAACTACAGGTATGAATCAGCTTGGCGCGCATTATGAAGGACA
CTGCATTATTGAAATTGGTGCAGTTGAATTAATAAATCGTCGTTACACAGGTAATAATTRTCATATTTATATTAAGCCAG
ATCGCCCCGNTGATCCTGATGCGATTAAAGTTCATGGAATTACTGATGAAATGTTAGCGGATAAACCTGAATTCAAAGAA
GTTGCCCAAGATTTTTTGGATTATATTAATGGCGCAGAATTGCTTATTCACAATGCCCCCTTTGACGTTGGATTTATGGA
TTATGAATTTCGTAAACTTAACCTCAATGTGAAAACTGACGATATTTGTTTGGTGACAGATACGCTACAAATGGCGCGTC
AGATGTATCCTGGAAAACGCAATAATTTGGATGCACTTTGTGATCGTTTAGGTATTGATAATAGCAAACGTACTTTGCAC
GGTGCGTTACTGGATGCCGAGATCTTGGCGGATGTTTATTTGATGATGACGGGAGGGCAAACAAATTTATTCGATGAAGA
AGAATCTGTTGAGTCTGGAGTTATCCGTGTTATGCAAGAAAAAACGGCGGAAGAAATAAAAAGTGCGGTAGATTTTTCGC
ACAATTTGAAATTACTTCAACCTACTAATGATGAGCTACAAGCGCATTTAGAATTCTTAAAGATGATGAATAAAAAAAGT
GGAAATAATTGCCTTTGGGATAAACGTTTTGGCAATAATAATGTGCATTAA

Upstream 100 bases:

>100_bases
AGCAAGATCCATCAGTAAAAATTTCAATCTGTTTMGGCATAACAAAAAGGTTGTTGTGTAGAATGGCAAGTATTTTAATG
GAAAGAGATAGGTTGAACAA

Downstream 100 bases:

>100_bases
TCGTGTGGAATTTAAGCAGGTAAGTAAAATTTAATAAAAAAAGATTGACGAGATTTGTGTTCATCATTAAGATACGCACC
ACTTAGCGGAGTGGTAGTTC

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MINPNRQIVLDTETTGMNQLGAHYEGHCIIEIGAVELINRRYTGNNXHIYIKPDRPXDPDAIKVHGITDEMLADKPEFKE
VAQDFLDYINGAELLIHNAPFDVGFMDYEFRKLNLNVKTDDICLVTDTLQMARQMYPGKRNNLDALCDRLGIDNSKRTLH
GALLDAEILADVYLMMTGGQTNLFDEEESVESGVIRVMQEKTAEEIKSAVDFSHNLKLLQPTNDELQAHLEFLKMMNKKS
GNNCLWDKRFGNNNVH

Sequences:

>Translated_256_residues
MINPNRQIVLDTETTGMNQLGAHYEGHCIIEIGAVELINRRYTGNNXHIYIKPDRPXDPDAIKVHGITDEMLADKPEFKE
VAQDFLDYINGAELLIHNAPFDVGFMDYEFRKLNLNVKTDDICLVTDTLQMARQMYPGKRNNLDALCDRLGIDNSKRTLH
GALLDAEILADVYLMMTGGQTNLFDEEESVESGVIRVMQEKTAEEIKSAVDFSHNLKLLQPTNDELQAHLEFLKMMNKKS
GNNCLWDKRFGNNNVH
>Mature_256_residues
MINPNRQIVLDTETTGMNQLGAHYEGHCIIEIGAVELINRRYTGNNXHIYIKPDRPXDPDAIKVHGITDEMLADKPEFKE
VAQDFLDYINGAELLIHNAPFDVGFMDYEFRKLNLNVKTDDICLVTDTLQMARQMYPGKRNNLDALCDRLGIDNSKRTLH
GALLDAEILADVYLMMTGGQTNLFDEEESVESGVIRVMQEKTAEEIKSAVDFSHNLKLLQPTNDELQAHLEFLKMMNKKS
GNNCLWDKRFGNNNVH

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786409, Length=243, Percent_Identity=59.6707818930041, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1788149, Length=181, Percent_Identity=30.3867403314917, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPO3E_HAEIN (P43745)

Other databases:

- EMBL:   L42023
- PIR:   B64050
- RefSeq:   NP_438306.1
- ProteinModelPortal:   P43745
- GeneID:   951047
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0137
- NMPDR:   fig|71421.1.peg.128
- TIGR:   HI_0137
- HOGENOM:   HBG728612
- OMA:   HGITNEF
- ProtClustDB:   PRK05711
- BioCyc:   HINF71421:HI_0137-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005622
- InterPro:   IPR006054
- InterPro:   IPR006309
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR012337
- SMART:   SM00479
- TIGRFAMs:   TIGR00573
- TIGRFAMs:   TIGR01406

Pfam domain/function: PF00929 Exonuc_X-T; SSF53098 RNaseH_fold

EC number: =2.7.7.7

Molecular weight: Translated: 29025; Mature: 29025

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: ACT_SITE 160-160 BINDING 11-11 BINDING 13-13 BINDING 60-60 BINDING 65-65 BINDING 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPNRQIVLDTETTGMNQLGAHYEGHCIIEIGAVELINRRYTGNNXHIYIKPDRPXDPD
CCCCCCEEEEECCCCCHHHHCCCCCCEEEEEECHHHHHHHCCCCCCCEEEECCCCCCCCC
AIKVHGITDEMLADKPEFKEVAQDFLDYINGAELLIHNAPFDVGFMDYEFRKLNLNVKTD
CEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEECEEEEEEEEEECCC
DICLVTDTLQMARQMYPGKRNNLDALCDRLGIDNSKRTLHGALLDAEILADVYLMMTGGQ
CEEEEHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEECCC
TNLFDEEESVESGVIRVMQEKTAEEIKSAVDFSHNLKLLQPTNDELQAHLEFLKMMNKKS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCC
GNNCLWDKRFGNNNVH
CCCCEEECCCCCCCCC
>Mature Secondary Structure
MINPNRQIVLDTETTGMNQLGAHYEGHCIIEIGAVELINRRYTGNNXHIYIKPDRPXDPD
CCCCCCEEEEECCCCCHHHHCCCCCCEEEEEECHHHHHHHCCCCCCCEEEECCCCCCCCC
AIKVHGITDEMLADKPEFKEVAQDFLDYINGAELLIHNAPFDVGFMDYEFRKLNLNVKTD
CEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEECEEEEEEEEEECCC
DICLVTDTLQMARQMYPGKRNNLDALCDRLGIDNSKRTLHGALLDAEILADVYLMMTGGQ
CEEEEHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEECCC
TNLFDEEESVESGVIRVMQEKTAEEIKSAVDFSHNLKLLQPTNDELQAHLEFLKMMNKKS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCC
GNNCLWDKRFGNNNVH
CCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800