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The map label for this gene is thrA

Identifier: 16272063

GI number: 16272063

Start: 94425

End: 96872

Strand: Reverse

Name: thrA

Synonym: HI0089

Alternate gene names: 16272063

Gene position: 96872-94425 (Counterclockwise)

Preceding gene: 16272065

Following gene: 16272062

Centisome position: 5.29

GC content: 40.52

Gene sequence:

>2448_bases
ATGCGCGTACTTAAATTTGGCGGCACTTCGCTTGCAAATCCGGAACGTTTTTCCCAAGCGGCGAAATTGATCGAGCAAGC
TCATTTAGAAGAGCAGGCTGCAGGTGTTTTATCTGCACCAGCCAAAATAACCAACCATCTCGTCGCCCTTTCTGAAAAAG
CAGCATTAAATCAATCAACCGATACGCATTTCAACGAAGCAATCGAAATTTTCTATAACATCATCAACGGCTTACATACG
GAAAATAATCAATTCGACTTAAATGGTACCAAAGCACTTATTGATGCTGAATTTGTTCAAATTAAAGGATTATTAGAAGA
AATCCGTCAGGCAGGAAAAGTAGAAGATGCAGTTAAAGCGACCATTGATTGCCGTGGGGAAAAACTTTCTATTGCAATGA
TGAAAGCTTGGTTTGAAGCGCGTGGCTATAGCGTACATATTGTGGATCCTGTCAAACAATTATTGGCAAAAGGCGGTTAT
CTTGAATCTTCTGTTGAAATTGAAGAATCAACAAAACGTGTTGATGCAGCAAATATCGCAAAAGATAAAGTGGTGCTGAT
GGCAGGTTTTACTGCGGGTAATGAAAAAGGCGAGTTGGTATTGCTCGGTCGTAATGGCTCAGACTATTCTGCCGCCTGTT
TAGCCGCTTGTTTAGGCGCAAGTGTATGCGAAATTTGGACCGATGTGGACGGCGTTTATACTTGCGATCCGCGTTTAGTG
CCTGATGCACGTTTATTACCAACGCTTTCTTATCGTGAAGCGATGGAACTCTCTTATTTCGGTGCGAAAGTGATTCATCC
TCGTACGATTGGGCCATTGTTGCCACAAAATATTCCTTGTGTCATTAAAAATACAGGAAATCCTTCTGCACCAGGTTCGA
TTATTGATGGCAATGTGAAATCAGAAAGTTTGCAAGTTAAAGGTATCACTAACTTAGACAATTTGGCGATGTTTAATGTG
TCAGGCCCTGGTATGCAAGGTATGGTAGGCATGGCATCTCGCGTATTTTCAGCCATGTCAGGCGCAGGCATTTCAGTTAT
TTTAATTACTCAGTCTTCGTCTGAATATAGTATTAGTTTCTGTGTGCCTGTGAAATCTGCAGAAGTAGCGAAAACCGTAC
TAGAAACGGAGTTTGCTAATGAATTAAATGAACATCAATTAGAACCAATTGAAGTGATTAAAGACTTATCCATTATTTCT
GTTGTGGGCGATGGAATGAAGCAAGCGAAAGGCATTGCTGCTCGTTTCTTCTCGGCATTAGCGCAGGCAAACATTAGCAT
TGTGGCGATTGCACAAGGATCTAGCGAACGTTCAATTTCTGCGGTTGTGCCGCAAAATAAAGCCATTGAAGCGGTGAAGG
CTACCCATCAGGCGCTTTTCAATAATAAAAAAGTCGTGGATATGTTTTTAGTGGGCGTTGGTGGAGTAGGCGGAGAATTA
ATTGAGCAGGTAAAGCGTCAAAAGGAGTATCTAGCAAAGAAGAATGTTGAAATTCGAGTTTGTGCTATTGCGAATTCCAA
CCGTATGTTGCTTGATGAAAATGGATTAAATTTAGAGGATTGGAAAAACGATTTAGAAAATGCGACTCAGCCATCAGATT
TTGATGTATTGCTTTCTTTCATTAAATTACATCATGTGGTGAATCCAGTATTTGTAGATTGTACTTCAGCCGAATCTGTA
GCAGGGCTTTATGCTCGTGCGTTAAAAGAAGGTTTCCATGTGGTTACGCCAAACAAAAAAGCGAATACACGAGAATTAGT
TTATTACAATGAGTTACGCCAAAATGCCCAAGCAAGCCAGCATAAATTTTTATATGAAACCAATGTAGGTGCGGGCTTGC
CAGTTATTGAAAATCTCCAAAACTTACTTGCAGCAGGTGATGAGCTTGAATATTTTGAAGGGATTTTATCTGGCTCGCTT
TCCTTTATTTTTGGTAAGTTGGAAGAAGGACTTTCTCTTTCAGAAGTAACCGCACTTGCGCGTGAAAAAGGCTTTACTGA
GCCAGATCCTCGAGATGATCTTTCTGGACAAGATGTAGCACGCAAATTATTAATTCTTGCTCGTGAAGCGGGAATTGAAC
TTGAACTTTCAGATGTTGAAGTTGAAGGTGTATTGCCAAAAGGCTTCTCCGATGGAAAATCTGCAGATGAGTTTATGGCA
ATGTTGCCTCAGCTTGATGAAGAATTTAAAACTCGCGTTGCCACAGCTAAAGCAGAAGGCAAAGTATTGCGTTATGTTGG
AAAAATTAGTGAAGGAAAATGCAAAGTATCCATCGTTGCGGTAGATCTGAATAATCCACTTTATAAAGTGAAAGATGGTG
AAAATGCCTTGGCATTTTACACTCGCTATTATCAGCCGATTCCACTTTTATTACGTGGTTATGGCGCAGGTAATGCAGTT
ACTGCGGCAGGTATCTTTGCAGATATTTTAAGAACATTACAACATTAA

Upstream 100 bases:

>100_bases
CGGGTTAGTGCAGCAAAAACAAGATACAGAAAACCCGCGATTCAACTGAATAGCGGGTTTTTTTATAAATTTAAAAACAC
AATATCTAGGAGAACCAATT

Downstream 100 bases:

>100_bases
TAAGGATTCCCAATGTTAAGAATTTACGCCCCTGCATCTAGCGCGAATATTAGCGTAGGTTTCGATACTTTAGGTGCTGC
GATCTCTCCTATTGATGGTT

Product: bifunctional aspartokinase I/homoserine dehydrogenase I

Products: NA

Alternate protein names: AK-HD; Aspartokinase; Homoserine dehydrogenase

Number of amino acids: Translated: 815; Mature: 815

Protein sequence:

>815_residues
MRVLKFGGTSLANPERFSQAAKLIEQAHLEEQAAGVLSAPAKITNHLVALSEKAALNQSTDTHFNEAIEIFYNIINGLHT
ENNQFDLNGTKALIDAEFVQIKGLLEEIRQAGKVEDAVKATIDCRGEKLSIAMMKAWFEARGYSVHIVDPVKQLLAKGGY
LESSVEIEESTKRVDAANIAKDKVVLMAGFTAGNEKGELVLLGRNGSDYSAACLAACLGASVCEIWTDVDGVYTCDPRLV
PDARLLPTLSYREAMELSYFGAKVIHPRTIGPLLPQNIPCVIKNTGNPSAPGSIIDGNVKSESLQVKGITNLDNLAMFNV
SGPGMQGMVGMASRVFSAMSGAGISVILITQSSSEYSISFCVPVKSAEVAKTVLETEFANELNEHQLEPIEVIKDLSIIS
VVGDGMKQAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPQNKAIEAVKATHQALFNNKKVVDMFLVGVGGVGGEL
IEQVKRQKEYLAKKNVEIRVCAIANSNRMLLDENGLNLEDWKNDLENATQPSDFDVLLSFIKLHHVVNPVFVDCTSAESV
AGLYARALKEGFHVVTPNKKANTRELVYYNELRQNAQASQHKFLYETNVGAGLPVIENLQNLLAAGDELEYFEGILSGSL
SFIFGKLEEGLSLSEVTALAREKGFTEPDPRDDLSGQDVARKLLILAREAGIELELSDVEVEGVLPKGFSDGKSADEFMA
MLPQLDEEFKTRVATAKAEGKVLRYVGKISEGKCKVSIVAVDLNNPLYKVKDGENALAFYTRYYQPIPLLLRGYGAGNAV
TAAGIFADILRTLQH

Sequences:

>Translated_815_residues
MRVLKFGGTSLANPERFSQAAKLIEQAHLEEQAAGVLSAPAKITNHLVALSEKAALNQSTDTHFNEAIEIFYNIINGLHT
ENNQFDLNGTKALIDAEFVQIKGLLEEIRQAGKVEDAVKATIDCRGEKLSIAMMKAWFEARGYSVHIVDPVKQLLAKGGY
LESSVEIEESTKRVDAANIAKDKVVLMAGFTAGNEKGELVLLGRNGSDYSAACLAACLGASVCEIWTDVDGVYTCDPRLV
PDARLLPTLSYREAMELSYFGAKVIHPRTIGPLLPQNIPCVIKNTGNPSAPGSIIDGNVKSESLQVKGITNLDNLAMFNV
SGPGMQGMVGMASRVFSAMSGAGISVILITQSSSEYSISFCVPVKSAEVAKTVLETEFANELNEHQLEPIEVIKDLSIIS
VVGDGMKQAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPQNKAIEAVKATHQALFNNKKVVDMFLVGVGGVGGEL
IEQVKRQKEYLAKKNVEIRVCAIANSNRMLLDENGLNLEDWKNDLENATQPSDFDVLLSFIKLHHVVNPVFVDCTSAESV
AGLYARALKEGFHVVTPNKKANTRELVYYNELRQNAQASQHKFLYETNVGAGLPVIENLQNLLAAGDELEYFEGILSGSL
SFIFGKLEEGLSLSEVTALAREKGFTEPDPRDDLSGQDVARKLLILAREAGIELELSDVEVEGVLPKGFSDGKSADEFMA
MLPQLDEEFKTRVATAKAEGKVLRYVGKISEGKCKVSIVAVDLNNPLYKVKDGENALAFYTRYYQPIPLLLRGYGAGNAV
TAAGIFADILRTLQH
>Mature_815_residues
MRVLKFGGTSLANPERFSQAAKLIEQAHLEEQAAGVLSAPAKITNHLVALSEKAALNQSTDTHFNEAIEIFYNIINGLHT
ENNQFDLNGTKALIDAEFVQIKGLLEEIRQAGKVEDAVKATIDCRGEKLSIAMMKAWFEARGYSVHIVDPVKQLLAKGGY
LESSVEIEESTKRVDAANIAKDKVVLMAGFTAGNEKGELVLLGRNGSDYSAACLAACLGASVCEIWTDVDGVYTCDPRLV
PDARLLPTLSYREAMELSYFGAKVIHPRTIGPLLPQNIPCVIKNTGNPSAPGSIIDGNVKSESLQVKGITNLDNLAMFNV
SGPGMQGMVGMASRVFSAMSGAGISVILITQSSSEYSISFCVPVKSAEVAKTVLETEFANELNEHQLEPIEVIKDLSIIS
VVGDGMKQAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPQNKAIEAVKATHQALFNNKKVVDMFLVGVGGVGGEL
IEQVKRQKEYLAKKNVEIRVCAIANSNRMLLDENGLNLEDWKNDLENATQPSDFDVLLSFIKLHHVVNPVFVDCTSAESV
AGLYARALKEGFHVVTPNKKANTRELVYYNELRQNAQASQHKFLYETNVGAGLPVIENLQNLLAAGDELEYFEGILSGSL
SFIFGKLEEGLSLSEVTALAREKGFTEPDPRDDLSGQDVARKLLILAREAGIELELSDVEVEGVLPKGFSDGKSADEFMA
MLPQLDEEFKTRVATAKAEGKVLRYVGKISEGKCKVSIVAVDLNNPLYKVKDGENALAFYTRYYQPIPLLLRGYGAGNAV
TAAGIFADILRTLQH

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains

Homologues:

Organism=Escherichia coli, GI1786183, Length=814, Percent_Identity=62.2850122850123, Blast_Score=1009, Evalue=0.0,
Organism=Escherichia coli, GI1790376, Length=817, Percent_Identity=29.2533659730722, Blast_Score=326, Evalue=3e-90,
Organism=Escherichia coli, GI1790455, Length=467, Percent_Identity=28.4796573875803, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322599, Length=338, Percent_Identity=35.5029585798817, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320893, Length=465, Percent_Identity=30.1075268817204, Blast_Score=156, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AKH_HAEIN (P44505)

Other databases:

- EMBL:   L42023
- PIR:   A64048
- RefSeq:   NP_438262.1
- ProteinModelPortal:   P44505
- GeneID:   950991
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0089
- NMPDR:   fig|71421.1.peg.88
- TIGR:   HI_0089
- HOGENOM:   HBG298754
- OMA:   VKDGENA
- ProtClustDB:   PRK09436
- BioCyc:   HINF71421:HI_0089-MONOMER
- BRENDA:   1.1.1.3
- BRENDA:   2.7.2.4
- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.1160.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000727
- TIGRFAMs:   TIGR00657

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; PF00742 Homoserine_dh; PF03447 NAD_binding_3; SSF53633 Aa_kinase

EC number: =2.7.2.4; =1.1.1.3

Molecular weight: Translated: 88222; Mature: 88222

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00324 ASPARTOKINASE; PS01042 HOMOSER_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLKFGGTSLANPERFSQAAKLIEQAHLEEQAAGVLSAPAKITNHLVALSEKAALNQST
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
DTHFNEAIEIFYNIINGLHTENNQFDLNGTKALIDAEFVQIKGLLEEIRQAGKVEDAVKA
CHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHH
TIDCRGEKLSIAMMKAWFEARGYSVHIVDPVKQLLAKGGYLESSVEIEESTKRVDAANIA
HHCCCCCEEHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCC
KDKVVLMAGFTAGNEKGELVLLGRNGSDYSAACLAACLGASVCEIWTDVDGVYTCDPRLV
CCCEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCEEECCCCCC
PDARLLPTLSYREAMELSYFGAKVIHPRTIGPLLPQNIPCVIKNTGNPSAPGSIIDGNVK
CCCHHCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCEEECCCC
SESLQVKGITNLDNLAMFNVSGPGMQGMVGMASRVFSAMSGAGISVILITQSSSEYSISF
CCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEE
CVPVKSAEVAKTVLETEFANELNEHQLEPIEVIKDLSIISVVGDGMKQAKGIAARFFSAL
EEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AQANISIVAIAQGSSERSISAVVPQNKAIEAVKATHQALFNNKKVVDMFLVGVGGVGGEL
HHCCEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHH
IEQVKRQKEYLAKKNVEIRVCAIANSNRMLLDENGLNLEDWKNDLENATQPSDFDVLLSF
HHHHHHHHHHHHHCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHH
IKLHHVVNPVFVDCTSAESVAGLYARALKEGFHVVTPNKKANTRELVYYNELRQNAQASQ
HHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEHHHHHHHHHHHH
HKFLYETNVGAGLPVIENLQNLLAAGDELEYFEGILSGSLSFIFGKLEEGLSLSEVTALA
CCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHH
REKGFTEPDPRDDLSGQDVARKLLILAREAGIELELSDVEVEGVLPKGFSDGKSADEFMA
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECEEEEECCCCCCCCCCCHHHHHH
MLPQLDEEFKTRVATAKAEGKVLRYVGKISEGKCKVSIVAVDLNNPLYKVKDGENALAFY
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCHHHHH
TRYYQPIPLLLRGYGAGNAVTAAGIFADILRTLQH
HHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVLKFGGTSLANPERFSQAAKLIEQAHLEEQAAGVLSAPAKITNHLVALSEKAALNQST
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
DTHFNEAIEIFYNIINGLHTENNQFDLNGTKALIDAEFVQIKGLLEEIRQAGKVEDAVKA
CHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHH
TIDCRGEKLSIAMMKAWFEARGYSVHIVDPVKQLLAKGGYLESSVEIEESTKRVDAANIA
HHCCCCCEEHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCC
KDKVVLMAGFTAGNEKGELVLLGRNGSDYSAACLAACLGASVCEIWTDVDGVYTCDPRLV
CCCEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCEEECCCCCC
PDARLLPTLSYREAMELSYFGAKVIHPRTIGPLLPQNIPCVIKNTGNPSAPGSIIDGNVK
CCCHHCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCEEECCCC
SESLQVKGITNLDNLAMFNVSGPGMQGMVGMASRVFSAMSGAGISVILITQSSSEYSISF
CCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEE
CVPVKSAEVAKTVLETEFANELNEHQLEPIEVIKDLSIISVVGDGMKQAKGIAARFFSAL
EEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AQANISIVAIAQGSSERSISAVVPQNKAIEAVKATHQALFNNKKVVDMFLVGVGGVGGEL
HHCCEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHH
IEQVKRQKEYLAKKNVEIRVCAIANSNRMLLDENGLNLEDWKNDLENATQPSDFDVLLSF
HHHHHHHHHHHHHCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHH
IKLHHVVNPVFVDCTSAESVAGLYARALKEGFHVVTPNKKANTRELVYYNELRQNAQASQ
HHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEHHHHHHHHHHHH
HKFLYETNVGAGLPVIENLQNLLAAGDELEYFEGILSGSLSFIFGKLEEGLSLSEVTALA
CCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHH
REKGFTEPDPRDDLSGQDVARKLLILAREAGIELELSDVEVEGVLPKGFSDGKSADEFMA
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECEEEEECCCCCCCCCCCHHHHHH
MLPQLDEEFKTRVATAKAEGKVLRYVGKISEGKCKVSIVAVDLNNPLYKVKDGENALAFY
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCHHHHH
TRYYQPIPLLLRGYGAGNAVTAAGIFADILRTLQH
HHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800