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The map label for this gene is ppnK [H]

Identifier: 16272045

GI number: 16272045

Start: 77902

End: 78687

Strand: Reverse

Name: ppnK [H]

Synonym: HI0072

Alternate gene names: 16272045

Gene position: 78687-77902 (Counterclockwise)

Preceding gene: 16272047

Following gene: 16272044

Centisome position: 4.3

GC content: 38.17

Gene sequence:

>786_bases
ATGCACAAAAACTTATTTCATTGGTTAATGGAACGTGGTTATCAAGTGTTGGTGGAAAAAGAAGTCGCCATAACACTTGA
GTTACCTTTTGAACATCTTGCTACGTTAGAAGAAATAGGCCACCGAGCCCAATTAGCCATTGTGATTGGTGGAGACGGCA
ATATGCTAGGGCGCGCTCGCGTATTAGCAAAATATGATATTCCATTGATTGGTATTAATCGTGGTAATTTGGGATTTTTA
ACGGATATTGACCCGAAAAATGCCTATTCCCAGCTTGAAGCTTGTTTAGAACGTGGCGAATTTTTTGTGGAAGAACGTTT
TTTATTGGAAGCAAAAATCGAACGAGCAAGTGAAATCGTATCAACCAGCAATGCGGTAAATGAAGCGGTTATTCATCCCG
CCAAAATTGCACATATGATTGATTTTCACGTATATATCAATGATAAGTTTGCATTTTCTCAACGTTCTGATGGATTAATT
GTTTCTACTCCAACAGGTTCTACGGCTTATTCTCTTTCCGCTGGTGGACCTATTTTGACACCAAACCTTAATGCCATTGC
ATTAGTGCCAATGTTTCCACATACATTAACTTCTCGCCCTCTTGTTGTTGATGGGGATAGTAAAATATCGATTCGTTTTG
CTGAACATAATACCTCTCAATTAGAAGTGGGCTGTGATAGTCAAATTACCTTACCTTTTACCCCAGATGATGTGGTGCAT
ATTCAAAAAAGCGAGCATAAACTCCGATTGCTTCATCTGAAAATTATAATTATTACAATGTGTTAA

Upstream 100 bases:

>100_bases
AAATGTTAAATTCAAGTGCGGTCATTTTATGAATCATTTATATCGTTCCTTTAAAACCATTGCTCTTGTAGGTAAGCCTC
GCAATGATATTAACTTACAA

Downstream 100 bases:

>100_bases
GTAGCAAATTAGGTTGGTTAAAATCATTTTGATTATGGTAAAAATTTTCGATTGAAATAGATTTGACAAAATACTGTAAA
AAATCCTTTACTGTAAATTA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MHKNLFHWLMERGYQVLVEKEVAITLELPFEHLATLEEIGHRAQLAIVIGGDGNMLGRARVLAKYDIPLIGINRGNLGFL
TDIDPKNAYSQLEACLERGEFFVEERFLLEAKIERASEIVSTSNAVNEAVIHPAKIAHMIDFHVYINDKFAFSQRSDGLI
VSTPTGSTAYSLSAGGPILTPNLNAIALVPMFPHTLTSRPLVVDGDSKISIRFAEHNTSQLEVGCDSQITLPFTPDDVVH
IQKSEHKLRLLHLKIIIITMC

Sequences:

>Translated_261_residues
MHKNLFHWLMERGYQVLVEKEVAITLELPFEHLATLEEIGHRAQLAIVIGGDGNMLGRARVLAKYDIPLIGINRGNLGFL
TDIDPKNAYSQLEACLERGEFFVEERFLLEAKIERASEIVSTSNAVNEAVIHPAKIAHMIDFHVYINDKFAFSQRSDGLI
VSTPTGSTAYSLSAGGPILTPNLNAIALVPMFPHTLTSRPLVVDGDSKISIRFAEHNTSQLEVGCDSQITLPFTPDDVVH
IQKSEHKLRLLHLKIIIITMC
>Mature_261_residues
MHKNLFHWLMERGYQVLVEKEVAITLELPFEHLATLEEIGHRAQLAIVIGGDGNMLGRARVLAKYDIPLIGINRGNLGFL
TDIDPKNAYSQLEACLERGEFFVEERFLLEAKIERASEIVSTSNAVNEAVIHPAKIAHMIDFHVYINDKFAFSQRSDGLI
VSTPTGSTAYSLSAGGPILTPNLNAIALVPMFPHTLTSRPLVVDGDSKISIRFAEHNTSQLEVGCDSQITLPFTPDDVVH
IQKSEHKLRLLHLKIIIITMC

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=284, Percent_Identity=27.8169014084507, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788968, Length=253, Percent_Identity=58.498023715415, Blast_Score=302, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6320794, Length=220, Percent_Identity=30.9090909090909, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6325068, Length=186, Percent_Identity=31.7204301075269, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322509, Length=174, Percent_Identity=32.183908045977, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI28573826, Length=204, Percent_Identity=31.3725490196078, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28573832, Length=247, Percent_Identity=30.3643724696356, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28573828, Length=204, Percent_Identity=31.3725490196078, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28573830, Length=202, Percent_Identity=31.6831683168317, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161077047, Length=202, Percent_Identity=31.6831683168317, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363323, Length=229, Percent_Identity=28.3842794759825, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24653424, Length=229, Percent_Identity=28.3842794759825, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363321, Length=229, Percent_Identity=28.3842794759825, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129957, Length=229, Percent_Identity=28.3842794759825, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24653422, Length=229, Percent_Identity=28.3842794759825, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 29017; Mature: 29017

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKNLFHWLMERGYQVLVEKEVAITLELPFEHLATLEEIGHRAQLAIVIGGDGNMLGRAR
CCCHHHHHHHHCCEEEEEEEEEEEEEECCHHHHHHHHHCCCEEEEEEEECCCCCEECCEE
VLAKYDIPLIGINRGNLGFLTDIDPKNAYSQLEACLERGEFFVEERFLLEAKIERASEIV
EEEEECCCEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHH
STSNAVNEAVIHPAKIAHMIDFHVYINDKFAFSQRSDGLIVSTPTGSTAYSLSAGGPILT
HCCCHHHHHHCCHHHEEEEEEEEEEECCEEEECCCCCCEEEECCCCCCEEEECCCCCEEC
PNLNAIALVPMFPHTLTSRPLVVDGDSKISIRFAEHNTSQLEVGCDSQITLPFTPDDVVH
CCCCEEEEEECCCCHHCCCCEEEECCCEEEEEEECCCCCEEEECCCCEEEEECCCCCEEE
IQKSEHKLRLLHLKIIIITMC
EECCCCEEEEEEEEEEEEEEC
>Mature Secondary Structure
MHKNLFHWLMERGYQVLVEKEVAITLELPFEHLATLEEIGHRAQLAIVIGGDGNMLGRAR
CCCHHHHHHHHCCEEEEEEEEEEEEEECCHHHHHHHHHCCCEEEEEEEECCCCCEECCEE
VLAKYDIPLIGINRGNLGFLTDIDPKNAYSQLEACLERGEFFVEERFLLEAKIERASEIV
EEEEECCCEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHH
STSNAVNEAVIHPAKIAHMIDFHVYINDKFAFSQRSDGLIVSTPTGSTAYSLSAGGPILT
HCCCHHHHHHCCHHHEEEEEEEEEEECCEEEECCCCCCEEEECCCCCCEEEECCCCCEEC
PNLNAIALVPMFPHTLTSRPLVVDGDSKISIRFAEHNTSQLEVGCDSQITLPFTPDDVVH
CCCCEEEEEECCCCHHCCCCEEEECCCEEEEEEECCCCCEEEECCCCEEEEECCCCCEEE
IQKSEHKLRLLHLKIIIITMC
EECCCCEEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA