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The map label for this gene is potH [H]

Identifier: 162448123

GI number: 162448123

Start: 1342319

End: 1343158

Strand: Direct

Name: potH [H]

Synonym: ACL_1284

Alternate gene names: 162448123

Gene position: 1342319-1343158 (Clockwise)

Preceding gene: 162448122

Following gene: 162448124

Centisome position: 89.67

GC content: 30.95

Gene sequence:

>840_bases
ATGAAGAAACCATTTAGTCGACTATCCACACCATATATTGTATGGTTATTTATATTAGCACTTATCCCGATTGTTGTGAT
GTTATTTTTATCTGTTACAACAACTCAAGGTTTAAGTTTAGATGGTCTAACATTTAATTCATCCTTTTACCAAGCATTCT
TTGAAAGATCTATTACTACTGCATTCTTTAACTCAATCTATTATGCAATACTTACAACAGTTATTTCTCTACTATTAGGT
TATTTAGTTGCATATACTGTATTTAGATCTAAGTTTAAAAACAAGTTCTTAGTACTTGCAATCTTTATCTTACCAATGTG
GTCTAACCTTCTACTTAGAACAGAATCTCTTGGTAACTTAATGAATGAAAACAACTTGATTACAGATATCTTATCTAAAC
TATTAAATACTAATGTAAGTTTTCCAGTGATCAAAGGTAGTGGCTTAGCTGTCGTGATTGGTTTAGTCTTAACTTATCTA
CCATTTATGATCTTACCAATTTACACAGCTTTAGAAAAAATTGATTATAGTTTAGAAGAAGCTGCACTAGACTTAGGTCT
TACTGACCTACAAAAGTTTATGAAAGTTGTACTTCCACTTTCATTAAAAGGTGTTGCTACTGGTTCTATTCTAGTATTCT
TACCTGCAATGAGTGGCTTTGCTATTCCAGAAATATTAGGTTCTGGTAATATCTTATTAATTGGTAACATTATTGAACAA
TCCTTTAGATATATGGACTACAACTTAGGTTCCCTATTATCTATTATGGTCTTACTCATTATCTTCTTAGGTATCTTTGT
CGTAGCTAAGGTTGATAAGGAAGGAGAGATGCTTCTATGA

Upstream 100 bases:

>100_bases
TATAAGTTAATGGTAAACACCTACGAGACATATCAAGCAGGTGATACCATTGGATTAAAAATAGATCCTTATGAAATCCA
CTTAATGCCGGTATCAAATA

Downstream 100 bases:

>100_bases
CGCACTATCCAAAAGCAACCCTTGAATCCTACGGATACAAGGATTATAAGAAAACTAAAAAGTTTTTTACAGTTTTAAGA
AAAGTATTTGTTGTAGTGAT

Product: ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKKPFSRLSTPYIVWLFILALIPIVVMLFLSVTTTQGLSLDGLTFNSSFYQAFFERSITTAFFNSIYYAILTTVISLLLG
YLVAYTVFRSKFKNKFLVLAIFILPMWSNLLLRTESLGNLMNENNLITDILSKLLNTNVSFPVIKGSGLAVVIGLVLTYL
PFMILPIYTALEKIDYSLEEAALDLGLTDLQKFMKVVLPLSLKGVATGSILVFLPAMSGFAIPEILGSGNILLIGNIIEQ
SFRYMDYNLGSLLSIMVLLIIFLGIFVVAKVDKEGEMLL

Sequences:

>Translated_279_residues
MKKPFSRLSTPYIVWLFILALIPIVVMLFLSVTTTQGLSLDGLTFNSSFYQAFFERSITTAFFNSIYYAILTTVISLLLG
YLVAYTVFRSKFKNKFLVLAIFILPMWSNLLLRTESLGNLMNENNLITDILSKLLNTNVSFPVIKGSGLAVVIGLVLTYL
PFMILPIYTALEKIDYSLEEAALDLGLTDLQKFMKVVLPLSLKGVATGSILVFLPAMSGFAIPEILGSGNILLIGNIIEQ
SFRYMDYNLGSLLSIMVLLIIFLGIFVVAKVDKEGEMLL
>Mature_279_residues
MKKPFSRLSTPYIVWLFILALIPIVVMLFLSVTTTQGLSLDGLTFNSSFYQAFFERSITTAFFNSIYYAILTTVISLLLG
YLVAYTVFRSKFKNKFLVLAIFILPMWSNLLLRTESLGNLMNENNLITDILSKLLNTNVSFPVIKGSGLAVVIGLVLTYL
PFMILPIYTALEKIDYSLEEAALDLGLTDLQKFMKVVLPLSLKGVATGSILVFLPAMSGFAIPEILGSGNILLIGNIIEQ
SFRYMDYNLGSLLSIMVLLIIFLGIFVVAKVDKEGEMLL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=215, Percent_Identity=37.6744186046512, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1787369, Length=262, Percent_Identity=30.5343511450382, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787713, Length=182, Percent_Identity=31.3186813186813, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31034; Mature: 31034

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPFSRLSTPYIVWLFILALIPIVVMLFLSVTTTQGLSLDGLTFNSSFYQAFFERSITT
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHH
AFFNSIYYAILTTVISLLLGYLVAYTVFRSKFKNKFLVLAIFILPMWSNLLLRTESLGNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
MNENNLITDILSKLLNTNVSFPVIKGSGLAVVIGLVLTYLPFMILPIYTALEKIDYSLEE
HCCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALDLGLTDLQKFMKVVLPLSLKGVATGSILVFLPAMSGFAIPEILGSGNILLIGNIIEQ
HHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCEEEEEHHHHH
SFRYMDYNLGSLLSIMVLLIIFLGIFVVAKVDKEGEMLL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKKPFSRLSTPYIVWLFILALIPIVVMLFLSVTTTQGLSLDGLTFNSSFYQAFFERSITT
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHH
AFFNSIYYAILTTVISLLLGYLVAYTVFRSKFKNKFLVLAIFILPMWSNLLLRTESLGNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
MNENNLITDILSKLLNTNVSFPVIKGSGLAVVIGLVLTYLPFMILPIYTALEKIDYSLEE
HCCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALDLGLTDLQKFMKVVLPLSLKGVATGSILVFLPAMSGFAIPEILGSGNILLIGNIIEQ
HHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCEEEEEHHHHH
SFRYMDYNLGSLLSIMVLLIIFLGIFVVAKVDKEGEMLL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]