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The map label for this gene is amyA3 [H]

Identifier: 162447517

GI number: 162447517

Start: 684241

End: 686043

Strand: Direct

Name: amyA3 [H]

Synonym: ACL_0657

Alternate gene names: 162447517

Gene position: 684241-686043 (Clockwise)

Preceding gene: 162447514

Following gene: 162447525

Centisome position: 45.71

GC content: 31.45

Gene sequence:

>1803_bases
ATGAATAAACACGCATTATTTCATCAAGCAAAAAGTGAATACAGTTATGCTTATGATGATAAAAAACTTCATATATTATT
TAGAACCATGAAAGATGATGTCCAATCTGTAAAACTGTCATATGGTGATCCATTTGATTGGACTTATAAAAATAGAAAAG
CTCGTTGGGAGAGCCAATTAGTCGAGATGACTAAAAGATATTCTACCCATCTTTTTGATTATTACTTCATTGAGGTTGAA
CCAAAGGATTATCGCTGCAAGTATGCTTTTTTAATCGAAGCAGATGATAAACATTATTTTTATGGTTCAAAGCGTATGGA
AGAACTAGAAGGTCTACCACCTACGGATTTTGATGAAGGTAGTTTTTATGATTTAAGTAATTATTTTAACTATCCATATT
TAAACTATGAAGATCAAGCCAATACACCAAAATGGGTAAAAGATACTGTATGGTATCAAATATTTATGGATAGATTTTAT
AGTCATAGTAAGAAATCTAATTTACCCTGGGCAAAACTTCCTGTAAATAACCATGAATTCTATGGCGGTGATATTAAAGG
TGTTATAGACAAACTACCATATTTAAAGGATTTAGGTATATCTGGTATATATTTTACTCCGATTTTTGAATCTCGTACAG
CACATAAATATGATACTAAAGATTATTTTAAAATTGATCCACAATTTGGGACAAATGAAGACTTTAGATTATTAGTCCAA
GAAGCGCATAAATTAGGCATTAAAGTAGTGCTTGATGCTGTCTTAAACCACTGTGGTTGGGATCATCCGTTCTTTCAAGA
TGTTGTAAAATATGGTAGAAACTCTAAATATGCGGATTGTTTCTTTTTAGATAAAGATCCAATTCTAAATTTTGAACTTA
CTGAAGATGGTAGTCCTAAAGGACACGGTAATTTAAGACCAAATTTTAGAACGTTTGCTTATACACCATTTATGCCAAAA
TGGAATACAGGTAATGAGTTAGCTCAGAAACATTTACTTGATTCAATTGAGTTTTGGATTAAAGAATTTGACATTGATGG
CTGGAGACTGGATGTGTCTAACGAAGTTAGTCATGACTTCTTAAGAAAAGTTAAACAGGTATCCAGAGCTGCTAAAGCAG
ATACATTCATATTTGGTGAAAACTGGGATTCTTCGATGCCTTGGTTAAGAGGGGATCAAATGGATGCTGTAATGAACTAT
GATTTATCCATACCAATGTGGCAATATTTTGATGGAAAAATTGATTCGGAAGAGTTTAAAAATGAATTAACTCAATATAC
CGCATTGACTCCAAAACATGTTATGAAAAATATGTTTAATCAATTAGATACACATGATACAATCCGCATGAAAAGACGTT
TGAAAGATAATGATGAACGCTTAAAGATTGCATACATTCTAATGTTTGCCAGTGCCGGTGCACCAAATATCTATTATGGT
AGTGAGGTTGGCTTAACTGGTGAACACGATCCGGATAATAGACGCTGTTTTAATTGGGATGAGTCTACTTGGAATCATAG
TTTACATCAATTGATTAAAGACTTAATACAATTAAGAAAACAACATCCTGCATTTATGGAAAGTGATTATCAGTTTATTG
ATAAATCATCCATTTCATTTGTGAAACATCATAAAAATGAAAGTTTACTATTTCTAATTAATGATTCCGAAAAATCAGTT
AAACTAAACATAGATCCGGCATATCAATTACAATATACGGATGTATTTAGTAAAAAAGCTGTAAATGTACATGCAGAAGT
TGAACTTCCAGCATATGGTTATAAAATACTTAAATTCATTTAA

Upstream 100 bases:

>100_bases
TATTTTACCAAAATAACTTCTATACTAAAACATGATAGCGGTTACATATTTATCAATCAAATGACTAAATGATATAATGA
AATTCAAGGAGTGATATACC

Downstream 100 bases:

>100_bases
ACGTTAAGGTCCCTAAAGAAGAAATCTTTAAGGACCTTTTTTAGGTGTTATTTTAAATGAAATATAATAGGTTCGTTACG
AAGTGGTAATGAGCCATTTT

Product: alpha-amylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MNKHALFHQAKSEYSYAYDDKKLHILFRTMKDDVQSVKLSYGDPFDWTYKNRKARWESQLVEMTKRYSTHLFDYYFIEVE
PKDYRCKYAFLIEADDKHYFYGSKRMEELEGLPPTDFDEGSFYDLSNYFNYPYLNYEDQANTPKWVKDTVWYQIFMDRFY
SHSKKSNLPWAKLPVNNHEFYGGDIKGVIDKLPYLKDLGISGIYFTPIFESRTAHKYDTKDYFKIDPQFGTNEDFRLLVQ
EAHKLGIKVVLDAVLNHCGWDHPFFQDVVKYGRNSKYADCFFLDKDPILNFELTEDGSPKGHGNLRPNFRTFAYTPFMPK
WNTGNELAQKHLLDSIEFWIKEFDIDGWRLDVSNEVSHDFLRKVKQVSRAAKADTFIFGENWDSSMPWLRGDQMDAVMNY
DLSIPMWQYFDGKIDSEEFKNELTQYTALTPKHVMKNMFNQLDTHDTIRMKRRLKDNDERLKIAYILMFASAGAPNIYYG
SEVGLTGEHDPDNRRCFNWDESTWNHSLHQLIKDLIQLRKQHPAFMESDYQFIDKSSISFVKHHKNESLLFLINDSEKSV
KLNIDPAYQLQYTDVFSKKAVNVHAEVELPAYGYKILKFI

Sequences:

>Translated_600_residues
MNKHALFHQAKSEYSYAYDDKKLHILFRTMKDDVQSVKLSYGDPFDWTYKNRKARWESQLVEMTKRYSTHLFDYYFIEVE
PKDYRCKYAFLIEADDKHYFYGSKRMEELEGLPPTDFDEGSFYDLSNYFNYPYLNYEDQANTPKWVKDTVWYQIFMDRFY
SHSKKSNLPWAKLPVNNHEFYGGDIKGVIDKLPYLKDLGISGIYFTPIFESRTAHKYDTKDYFKIDPQFGTNEDFRLLVQ
EAHKLGIKVVLDAVLNHCGWDHPFFQDVVKYGRNSKYADCFFLDKDPILNFELTEDGSPKGHGNLRPNFRTFAYTPFMPK
WNTGNELAQKHLLDSIEFWIKEFDIDGWRLDVSNEVSHDFLRKVKQVSRAAKADTFIFGENWDSSMPWLRGDQMDAVMNY
DLSIPMWQYFDGKIDSEEFKNELTQYTALTPKHVMKNMFNQLDTHDTIRMKRRLKDNDERLKIAYILMFASAGAPNIYYG
SEVGLTGEHDPDNRRCFNWDESTWNHSLHQLIKDLIQLRKQHPAFMESDYQFIDKSSISFVKHHKNESLLFLINDSEKSV
KLNIDPAYQLQYTDVFSKKAVNVHAEVELPAYGYKILKFI
>Mature_600_residues
MNKHALFHQAKSEYSYAYDDKKLHILFRTMKDDVQSVKLSYGDPFDWTYKNRKARWESQLVEMTKRYSTHLFDYYFIEVE
PKDYRCKYAFLIEADDKHYFYGSKRMEELEGLPPTDFDEGSFYDLSNYFNYPYLNYEDQANTPKWVKDTVWYQIFMDRFY
SHSKKSNLPWAKLPVNNHEFYGGDIKGVIDKLPYLKDLGISGIYFTPIFESRTAHKYDTKDYFKIDPQFGTNEDFRLLVQ
EAHKLGIKVVLDAVLNHCGWDHPFFQDVVKYGRNSKYADCFFLDKDPILNFELTEDGSPKGHGNLRPNFRTFAYTPFMPK
WNTGNELAQKHLLDSIEFWIKEFDIDGWRLDVSNEVSHDFLRKVKQVSRAAKADTFIFGENWDSSMPWLRGDQMDAVMNY
DLSIPMWQYFDGKIDSEEFKNELTQYTALTPKHVMKNMFNQLDTHDTIRMKRRLKDNDERLKIAYILMFASAGAPNIYYG
SEVGLTGEHDPDNRRCFNWDESTWNHSLHQLIKDLIQLRKQHPAFMESDYQFIDKSSISFVKHHKNESLLFLINDSEKSV
KLNIDPAYQLQYTDVFSKKAVNVHAEVELPAYGYKILKFI

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=392, Percent_Identity=23.9795918367347, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786604, Length=468, Percent_Identity=30.982905982906, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1790687, Length=400, Percent_Identity=26, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1789995, Length=133, Percent_Identity=29.3233082706767, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322245, Length=231, Percent_Identity=32.034632034632, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6322241, Length=228, Percent_Identity=31.140350877193, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322021, Length=228, Percent_Identity=31.140350877193, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324416, Length=228, Percent_Identity=31.140350877193, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321726, Length=269, Percent_Identity=30.4832713754647, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319776, Length=230, Percent_Identity=30.4347826086957, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321731, Length=230, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24586587, Length=181, Percent_Identity=32.5966850828729, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24583745, Length=181, Percent_Identity=33.1491712707182, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24586599, Length=181, Percent_Identity=31.4917127071823, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45549022, Length=177, Percent_Identity=33.8983050847458, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24583747, Length=226, Percent_Identity=30.9734513274336, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583749, Length=226, Percent_Identity=30.9734513274336, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24586591, Length=184, Percent_Identity=31.5217391304348, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI221330053, Length=179, Percent_Identity=29.608938547486, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586597, Length=98, Percent_Identity=40.8163265306122, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24586593, Length=241, Percent_Identity=27.3858921161826, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24586589, Length=83, Percent_Identity=46.9879518072289, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 71182; Mature: 71182

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKHALFHQAKSEYSYAYDDKKLHILFRTMKDDVQSVKLSYGDPFDWTYKNRKARWESQL
CCCCHHHHHHHCCCCEEECCCEEEEEHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHH
VEMTKRYSTHLFDYYFIEVEPKDYRCKYAFLIEADDKHYFYGSKRMEELEGLPPTDFDEG
HHHHHHHHHHEEEEEEEEECCCCCEEEEEEEEEECCCEEECCCHHHHHHCCCCCCCCCCC
SFYDLSNYFNYPYLNYEDQANTPKWVKDTVWYQIFMDRFYSHSKKSNLPWAKLPVNNHEF
CEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCC
YGGDIKGVIDKLPYLKDLGISGIYFTPIFESRTAHKYDTKDYFKIDPQFGTNEDFRLLVQ
CCCCHHHHHHHCCCHHHCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHH
EAHKLGIKVVLDAVLNHCGWDHPFFQDVVKYGRNSKYADCFFLDKDPILNFELTEDGSPK
HHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCC
GHGNLRPNFRTFAYTPFMPKWNTGNELAQKHLLDSIEFWIKEFDIDGWRLDVSNEVSHDF
CCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHH
LRKVKQVSRAAKADTFIFGENWDSSMPWLRGDQMDAVMNYDLSIPMWQYFDGKIDSEEFK
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCHHHHCCCCCCHHHHH
NELTQYTALTPKHVMKNMFNQLDTHDTIRMKRRLKDNDERLKIAYILMFASAGAPNIYYG
HHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCEEEC
SEVGLTGEHDPDNRRCFNWDESTWNHSLHQLIKDLIQLRKQHPAFMESDYQFIDKSSISF
CCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHCCCHHHH
VKHHKNESLLFLINDSEKSVKLNIDPAYQLQYTDVFSKKAVNVHAEVELPAYGYKILKFI
HHHCCCCEEEEEEECCCCEEEEECCCCEEEEEHHHHCCCEEEEEEEEECCHHHHHHHHCC
>Mature Secondary Structure
MNKHALFHQAKSEYSYAYDDKKLHILFRTMKDDVQSVKLSYGDPFDWTYKNRKARWESQL
CCCCHHHHHHHCCCCEEECCCEEEEEHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHH
VEMTKRYSTHLFDYYFIEVEPKDYRCKYAFLIEADDKHYFYGSKRMEELEGLPPTDFDEG
HHHHHHHHHHEEEEEEEEECCCCCEEEEEEEEEECCCEEECCCHHHHHHCCCCCCCCCCC
SFYDLSNYFNYPYLNYEDQANTPKWVKDTVWYQIFMDRFYSHSKKSNLPWAKLPVNNHEF
CEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCC
YGGDIKGVIDKLPYLKDLGISGIYFTPIFESRTAHKYDTKDYFKIDPQFGTNEDFRLLVQ
CCCCHHHHHHHCCCHHHCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHH
EAHKLGIKVVLDAVLNHCGWDHPFFQDVVKYGRNSKYADCFFLDKDPILNFELTEDGSPK
HHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCC
GHGNLRPNFRTFAYTPFMPKWNTGNELAQKHLLDSIEFWIKEFDIDGWRLDVSNEVSHDF
CCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHH
LRKVKQVSRAAKADTFIFGENWDSSMPWLRGDQMDAVMNYDLSIPMWQYFDGKIDSEEFK
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCHHHHCCCCCCHHHHH
NELTQYTALTPKHVMKNMFNQLDTHDTIRMKRRLKDNDERLKIAYILMFASAGAPNIYYG
HHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCEEEC
SEVGLTGEHDPDNRRCFNWDESTWNHSLHQLIKDLIQLRKQHPAFMESDYQFIDKSSISF
CCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHCCCHHHH
VKHHKNESLLFLINDSEKSVKLNIDPAYQLQYTDVFSKKAVNVHAEVELPAYGYKILKFI
HHHCCCCEEEEEEECCCCEEEEECCCCEEEEEHHHHCCCEEEEEEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]