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The map label for this gene is ydjE [H]

Identifier: 162447278

GI number: 162447278

Start: 432611

End: 433570

Strand: Direct

Name: ydjE [H]

Synonym: ACL_0413

Alternate gene names: 162447278

Gene position: 432611-433570 (Clockwise)

Preceding gene: 162447277

Following gene: 162447279

Centisome position: 28.9

GC content: 33.23

Gene sequence:

>960_bases
ATGAAAGTTATAACAATAGGTGAACTATTAATAGATTTCATACCTAAGGAAAAAGGTGTAAATCTAAAAGGAGTCCAAAA
CTTTATAAAACATGCAGGTGGAGCACCAGCAAATGTAGCAGCAGTGGTTGCTAAACTTGGTGGTGAGTCTATATTTTTAG
GACAAGTTGGTCATGACAGTTTCGGTAGTTATTTGATTGATAAACTAAAATCATTTAATGTAGAAACTAAATATATTCAC
CAAACATCTAAAAGACCAACATCACTGGCGTTTGTGAGTTTAACAGATGTAGGTGATAGAGATTTTGTATTCTATAGAAA
TCCCGGTGCGGATGAATTATATGAAGCATCTATGGTGCCTAAAAAAGAATTTGACAGAAACATCCTACATTTTTGCTCTG
TATCACTAACAGATAACCCAATTAAAGAAGCACACATCAAAGCGATTGAACTTACTAGAAAGCATAACGGATTAGTAAGC
TTTGATCCAAATATTAGACTGGCGTTATGGCAAGACCATAAAAAAATGCTGGATGTCATTTATGAATTCTTACACTTAAC
AGATATCGTTAAAGTATCTAGTGATGAACTAAACTTTATGACAGGCTTTGATGATGAACAAGTAGCGATTAAATCCCTAT
TTGTTGGTCAAGTGAAAGTAGTCATCGTGACTAAAGGTAAAGAAGGTTCTAGACTATACTTTAAAGATATTGATGCAGTC
ATTAAACATCCTGGATTTACAATAAATAGTATTGATACCACAGGTGCAGGCGATGCATTTATGGGTGCCTTCCTATATCA
ACTCAGTAAAAACAATCTTATCTTAAATCAATATAACAGTTATGACATTTTAAAATTTGCAAATGCCTATGCAGCACTAT
CTACTACAAAACTAGGAGCAATGGAAAATATTCCTAGTCTTGAAGAAGTTGATGCAATGATTAATAACTACAAAGAATGA

Upstream 100 bases:

>100_bases
AATACGATAAATACGATCCAATCGGATTGTATTTTTATTAGAAGCATTAAACATTTACCGGTTTAGTGATAAAATAGAAG
TATATGGGGAGGTTATACTT

Downstream 100 bases:

>100_bases
TGTTTAATAGCCTTATAAGGCACTTATACGCCGATTATCAACCGTATTCACTATTCAAATAAGACCGCTTTCGCGGCCTA
ACTAAGGTTTCAAATGTATA

Product: carbohydrate and purine kinase family protein

Products: ADP; D-fructose 6-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKVITIGELLIDFIPKEKGVNLKGVQNFIKHAGGAPANVAAVVAKLGGESIFLGQVGHDSFGSYLIDKLKSFNVETKYIH
QTSKRPTSLAFVSLTDVGDRDFVFYRNPGADELYEASMVPKKEFDRNILHFCSVSLTDNPIKEAHIKAIELTRKHNGLVS
FDPNIRLALWQDHKKMLDVIYEFLHLTDIVKVSSDELNFMTGFDDEQVAIKSLFVGQVKVVIVTKGKEGSRLYFKDIDAV
IKHPGFTINSIDTTGAGDAFMGAFLYQLSKNNLILNQYNSYDILKFANAYAALSTTKLGAMENIPSLEEVDAMINNYKE

Sequences:

>Translated_319_residues
MKVITIGELLIDFIPKEKGVNLKGVQNFIKHAGGAPANVAAVVAKLGGESIFLGQVGHDSFGSYLIDKLKSFNVETKYIH
QTSKRPTSLAFVSLTDVGDRDFVFYRNPGADELYEASMVPKKEFDRNILHFCSVSLTDNPIKEAHIKAIELTRKHNGLVS
FDPNIRLALWQDHKKMLDVIYEFLHLTDIVKVSSDELNFMTGFDDEQVAIKSLFVGQVKVVIVTKGKEGSRLYFKDIDAV
IKHPGFTINSIDTTGAGDAFMGAFLYQLSKNNLILNQYNSYDILKFANAYAALSTTKLGAMENIPSLEEVDAMINNYKE
>Mature_319_residues
MKVITIGELLIDFIPKEKGVNLKGVQNFIKHAGGAPANVAAVVAKLGGESIFLGQVGHDSFGSYLIDKLKSFNVETKYIH
QTSKRPTSLAFVSLTDVGDRDFVFYRNPGADELYEASMVPKKEFDRNILHFCSVSLTDNPIKEAHIKAIELTRKHNGLVS
FDPNIRLALWQDHKKMLDVIYEFLHLTDIVKVSSDELNFMTGFDDEQVAIKSLFVGQVKVVIVTKGKEGSRLYFKDIDAV
IKHPGFTINSIDTTGAGDAFMGAFLYQLSKNNLILNQYNSYDILKFANAYAALSTTKLGAMENIPSLEEVDAMINNYKE

Specific function: Unknown

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Homo sapiens, GI11545855, Length=308, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI87082343, Length=321, Percent_Identity=26.1682242990654, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082281, Length=279, Percent_Identity=26.1648745519713, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790193, Length=294, Percent_Identity=24.4897959183673, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532905, Length=325, Percent_Identity=25.8461538461538, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20128807, Length=287, Percent_Identity=26.8292682926829, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.4

Molecular weight: Translated: 35525; Mature: 35525

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVITIGELLIDFIPKEKGVNLKGVQNFIKHAGGAPANVAAVVAKLGGESIFLGQVGHDS
CEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCH
FGSYLIDKLKSFNVETKYIHQTSKRPTSLAFVSLTDVGDRDFVFYRNPGADELYEASMVP
HHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEEECCCCCCEEEEECCCHHHHHHHHCCC
KKEFDRNILHFCSVSLTDNPIKEAHIKAIELTRKHNGLVSFDPNIRLALWQDHKKMLDVI
HHHHHHHHHHHEEECCCCCCHHHHHHHHEEEEHHCCCEEEECCCEEEEEEHHHHHHHHHH
YEFLHLTDIVKVSSDELNFMTGFDDEQVAIKSLFVGQVKVVIVTKGKEGSRLYFKDIDAV
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCEEEEEEEECCCCCCEEHHHHHHHH
IKHPGFTINSIDTTGAGDAFMGAFLYQLSKNNLILNQYNSYDILKFANAYAALSTTKLGA
HCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCH
MENIPSLEEVDAMINNYKE
HHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKVITIGELLIDFIPKEKGVNLKGVQNFIKHAGGAPANVAAVVAKLGGESIFLGQVGHDS
CEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCH
FGSYLIDKLKSFNVETKYIHQTSKRPTSLAFVSLTDVGDRDFVFYRNPGADELYEASMVP
HHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEEECCCCCCEEEEECCCHHHHHHHHCCC
KKEFDRNILHFCSVSLTDNPIKEAHIKAIELTRKHNGLVSFDPNIRLALWQDHKKMLDVI
HHHHHHHHHHHEEECCCCCCHHHHHHHHEEEEHHCCCEEEECCCEEEEEEHHHHHHHHHH
YEFLHLTDIVKVSSDELNFMTGFDDEQVAIKSLFVGQVKVVIVTKGKEGSRLYFKDIDAV
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCEEEEEEEECCCCCCEEHHHHHHHH
IKHPGFTINSIDTTGAGDAFMGAFLYQLSKNNLILNQYNSYDILKFANAYAALSTTKLGA
HCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCH
MENIPSLEEVDAMINNYKE
HHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-fructose

Specific reaction: ATP + D-fructose = ADP + D-fructose 6-phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]