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The map label for this gene is ruvA

Identifier: 162447235

GI number: 162447235

Start: 388990

End: 389550

Strand: Direct

Name: ruvA

Synonym: ACL_0369

Alternate gene names: 162447235

Gene position: 388990-389550 (Clockwise)

Preceding gene: 162447233

Following gene: 162447236

Centisome position: 25.98

GC content: 28.52

Gene sequence:

>561_bases
ATGTATCGATACATTAAAGGTATAGTAACACAAATTAATCCACAACATATTGTTGTTGAAAATAATGGTGTGGGCTATTT
AGTCTTAAGTCCAGTACCATATCAATATAAAATTGGTGAAGAAACAACAGTAGTAACTTATTTACATGTAAGAGAGGATA
TTTTTCAACTTTATGGATTTAAAGATGAAGAAACTTTAAATCTATTTTTAAAACTTATAAGTGTATCTGGTATTGGTCCA
AAATCTGCAATGAGTATTGTAGCATTTGATGATACAAATAAAATTATTGCAGCCATTGAAACATCAGATGCAAAATATTT
AACTAAGTTTCCTGGTATTGGGATGAAGAGTGCTCAACAAATTATTTTAGACTTAAAAGGTAAACTGGTAAATGATGAAC
TAGATATGCAACTTTTAAGTGATAACTCTAAGGATGTAGCTGCTGCATTAGAAGCATTAGGCTATAATAAAAAAGAGATT
GCAAAATCATTAAAGCATGTTAATTTTGATCAAGACTTAAATAAAGCACTAAAAGAAGCACTTGCAATATTATTAAAGTA
G

Upstream 100 bases:

>100_bases
TATATAGCCATAAAACAATGTAAAAAAGGCACATATTGGTCTAAAAAGTCCGTAAAAATGTTATAATTAAATTTAAAGAT
AATAAAGTTAGGTGAAATCT

Downstream 100 bases:

>100_bases
GTATGACACATGAGTGAAGAAAAATCAGTATTAAGAGATTTAAATTTAAAAAATGATGATGAATTGATGCTTCGTCCTCA
AACCTTAAACCAATATATAG

Product: holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MYRYIKGIVTQINPQHIVVENNGVGYLVLSPVPYQYKIGEETTVVTYLHVREDIFQLYGFKDEETLNLFLKLISVSGIGP
KSAMSIVAFDDTNKIIAAIETSDAKYLTKFPGIGMKSAQQIILDLKGKLVNDELDMQLLSDNSKDVAAALEALGYNKKEI
AKSLKHVNFDQDLNKALKEALAILLK

Sequences:

>Translated_186_residues
MYRYIKGIVTQINPQHIVVENNGVGYLVLSPVPYQYKIGEETTVVTYLHVREDIFQLYGFKDEETLNLFLKLISVSGIGP
KSAMSIVAFDDTNKIIAAIETSDAKYLTKFPGIGMKSAQQIILDLKGKLVNDELDMQLLSDNSKDVAAALEALGYNKKEI
AKSLKHVNFDQDLNKALKEALAILLK
>Mature_186_residues
MYRYIKGIVTQINPQHIVVENNGVGYLVLSPVPYQYKIGEETTVVTYLHVREDIFQLYGFKDEETLNLFLKLISVSGIGP
KSAMSIVAFDDTNKIIAAIETSDAKYLTKFPGIGMKSAQQIILDLKGKLVNDELDMQLLSDNSKDVAAALEALGYNKKEI
AKSLKHVNFDQDLNKALKEALAILLK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:
GO:0000166: Holliday junction ATP-dependent DNA helicase ruvA
GO:0003677: Holliday junction ATP-dependent DNA helicase ruvA
GO:0003678: Holliday junction ATP-dependent DNA helicase ruvA
GO:0004386: Holliday junction ATP-dependent DNA helicase ruvA
GO:0005524: Holliday junction ATP-dependent DNA helicase ruvA
GO:0006281: Holliday junction ATP-dependent DNA helicase ruvA
GO:0006310: Holliday junction ATP-dependent DNA helicase ruvA
GO:0006974: Holliday junction ATP-dependent DNA helicase ruvA
GO:0009378: Holliday junction ATP-dependent DNA helicase ruvA
GO:0009379: Holliday junction ATP-dependent DNA helicase ruvA
GO:0009432: Holliday junction ATP-dependent DNA helicase ruvA
GO:0016787: Holliday junction ATP-dependent DNA helicase ruvA

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=199, Percent_Identity=32.1608040201005, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_ACHLI (A9NF61)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620367.1
- ProteinModelPortal:   A9NF61
- SMR:   A9NF61
- GeneID:   5804367
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0369
- HOGENOM:   HBG635309
- OMA:   FRERILF
- BioCyc:   ALAI441768:ACL_0369-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 20723; Mature: 20723

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRYIKGIVTQINPQHIVVENNGVGYLVLSPVPYQYKIGEETTVVTYLHVREDIFQLYGF
CCHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCEEECCCCCEEEEEEHHHHHHHHHHCC
KDEETLNLFLKLISVSGIGPKSAMSIVAFDDTNKIIAAIETSDAKYLTKFPGIGMKSAQQ
CCCHHHHHHHHHHHHCCCCCHHHEEEEEECCCCEEEEEEECCCCHHHHHCCCCCHHHHHH
IILDLKGKLVNDELDMQLLSDNSKDVAAALEALGYNKKEIAKSLKHVNFDQDLNKALKEA
HHHHHCCEEECCHHHHHEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHH
LAILLK
HHHHCC
>Mature Secondary Structure
MYRYIKGIVTQINPQHIVVENNGVGYLVLSPVPYQYKIGEETTVVTYLHVREDIFQLYGF
CCHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCEEECCCCCEEEEEEHHHHHHHHHHCC
KDEETLNLFLKLISVSGIGPKSAMSIVAFDDTNKIIAAIETSDAKYLTKFPGIGMKSAQQ
CCCHHHHHHHHHHHHCCCCCHHHEEEEEECCCCEEEEEEECCCCHHHHHCCCCCHHHHHH
IILDLKGKLVNDELDMQLLSDNSKDVAAALEALGYNKKEIAKSLKHVNFDQDLNKALKEA
HHHHHCCEEECCHHHHHEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHH
LAILLK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA