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The map label for this gene is topA [H]

Identifier: 162447205

GI number: 162447205

Start: 351025

End: 352812

Strand: Direct

Name: topA [H]

Synonym: ACL_0339

Alternate gene names: 162447205

Gene position: 351025-352812 (Clockwise)

Preceding gene: 162447204

Following gene: 162447206

Centisome position: 23.45

GC content: 32.44

Gene sequence:

>1788_bases
ATGTCAGACAAAGTAATTATTGTAGAATCACCTGCAAAATCAAAAACTATCTCAAGTTATTTCAACGATGAAGTAACTGT
TTTATCATCGGTAGGTCATATTAGAGACCTAGCAACCTCTGGTAAGGGTGGTTTAGGTGTAGATGTAGATAACAATTTTG
AACCTACCTATAAAATCATTACTGGTAAAGGTAAACTGATTACTGATTTAAAGAAAAAAACAAAAGGTAAAGAAGTTTTA
ATTGCAACCGACCCGGACCGCGAAGGAGAAGCTATCGCTTGGCATATCGCTGATGAGTTAGGTCTAGATTTAAACGAACA
TAACCGTATTGTTTTTAAAGAAATCACAAAACCTGCAGTTATGGAAGCAATCAACCATAAACGTCAAATCGATTTAGGAT
TAGTCAACTCTCAAGAAAGTAGACGTATTTTAGACAGAATCATTGGTTTTAAACTATCTGCATTTTTAAGAGACAAAATT
AAATCCAGATCAGCTGGTCGTGTTCAAAGTGTGGCTTTAAAACTAATTGTGGACTTAGAACGTGAAATTGAAAAATTTGT
GCCTGAAACATATTTCACCATGCATGCACATTTTAATGACCATGTAGCAGATTATGTTATTCCAAAAGATACACGTATTG
GATTAGAAGAAGCTACTAAAATTGTTGAAACATCAACAAATCCATTTTTAGTAAGTGATATTACTCAAAAAGAAACAAAA
AGAAACGCTAAACCACCTTACATGACATCTACCTTACTTCAAGATGGCGTAAACAGTCTATATATGAGCTCTAGCCGTGT
GATGTCAGTTGCATCTGAACTTTATCAAGGTATTGAAATCAATGGTCAAATTATCGGTTTAATCACGTATATGAAAACAG
ACTCTAATCGTTTATCTAAAGAGTTTGTAAATCCTGCAAAAGCATTCATTGAAACAAACTATGGAAAAGAATATGTTGGT
TTTTATAAGACAAATAATAAAGACTCTAGTCAGGATGCCCACGAAGCAATCAGACCTACAGATATTCATTTAACACCAGA
AAAAATAGAGCAATACTTATCAAAAGACCAATTTAAACTTTATAAGAGAATTTATGAACGTGCAGTAGGATCACTTATGG
CACCTGCAGTATTTAACAATACTAAAATTACGCTAGATTCAAATGGCAATATTTATACAATGGATGGAAGTATTCAAGTA
TTTGATGGTCACTTAAAAGTAACAGGTGATAAATCAAAAGATAAATTCTTACCTGAATATAAAATTGGTGATAAACTAAA
TGCTGATTTAGTTGAAAAGATTGAAAAACAAACCCAACCACCTGCTCGTTTTAATGAAGCATCACTCATTAAAGCATTAG
AAGCAAACGGTATTGGTAGACCATCTACATATGCAAGTATCATTAAAACGATTAAAGCACGCGACTATGTTATTGTAGAT
GAAAAACGATTTATTCCAACAGAACAAGGAAAGCTGACAGTAGATACCTTGAATCAATACTTTAATAAAATCATGAAAAT
TGATTATACATCTAATATGGAATCAGAACTTGACTTAATTGCAGATGGTAAAGTTGATCATATTCAATTATTGAAAGATT
TTTACAATAAATTTATGCCGATTTTAGAAGCTGCTCAACAAAATATGGAAAAAATAAAACCGGTTGTAACTGATAAAATA
TGTCCACTGTGCGGTGGAAACCTAGTTATTAGAAAATCTAGATATGGTGAGTTCTATGGTTGCAGCAATTTTCCGAAGTG
TAAACACATTGAACAGATGTCGGAATAA

Upstream 100 bases:

>100_bases
ATATTTATATAGAATAAAATATAAGAAAATTGAATACTTGACATCAAGAATTTTAATTATATAATTGATAAGGTATAAAA
CTAAAGAATTGAGGCAATCT

Downstream 100 bases:

>100_bases
ATGTGTTATTAAATACATAAATACGCATTAAACTTTACATAGAAAAAGAATAAGAATATAATGAAAATGCATCCAAACTA
TTGGGTGCTACTTATCCCCT

Product: DNA topoisomerase IA

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 595; Mature: 594

Protein sequence:

>595_residues
MSDKVIIVESPAKSKTISSYFNDEVTVLSSVGHIRDLATSGKGGLGVDVDNNFEPTYKIITGKGKLITDLKKKTKGKEVL
IATDPDREGEAIAWHIADELGLDLNEHNRIVFKEITKPAVMEAINHKRQIDLGLVNSQESRRILDRIIGFKLSAFLRDKI
KSRSAGRVQSVALKLIVDLEREIEKFVPETYFTMHAHFNDHVADYVIPKDTRIGLEEATKIVETSTNPFLVSDITQKETK
RNAKPPYMTSTLLQDGVNSLYMSSSRVMSVASELYQGIEINGQIIGLITYMKTDSNRLSKEFVNPAKAFIETNYGKEYVG
FYKTNNKDSSQDAHEAIRPTDIHLTPEKIEQYLSKDQFKLYKRIYERAVGSLMAPAVFNNTKITLDSNGNIYTMDGSIQV
FDGHLKVTGDKSKDKFLPEYKIGDKLNADLVEKIEKQTQPPARFNEASLIKALEANGIGRPSTYASIIKTIKARDYVIVD
EKRFIPTEQGKLTVDTLNQYFNKIMKIDYTSNMESELDLIADGKVDHIQLLKDFYNKFMPILEAAQQNMEKIKPVVTDKI
CPLCGGNLVIRKSRYGEFYGCSNFPKCKHIEQMSE

Sequences:

>Translated_595_residues
MSDKVIIVESPAKSKTISSYFNDEVTVLSSVGHIRDLATSGKGGLGVDVDNNFEPTYKIITGKGKLITDLKKKTKGKEVL
IATDPDREGEAIAWHIADELGLDLNEHNRIVFKEITKPAVMEAINHKRQIDLGLVNSQESRRILDRIIGFKLSAFLRDKI
KSRSAGRVQSVALKLIVDLEREIEKFVPETYFTMHAHFNDHVADYVIPKDTRIGLEEATKIVETSTNPFLVSDITQKETK
RNAKPPYMTSTLLQDGVNSLYMSSSRVMSVASELYQGIEINGQIIGLITYMKTDSNRLSKEFVNPAKAFIETNYGKEYVG
FYKTNNKDSSQDAHEAIRPTDIHLTPEKIEQYLSKDQFKLYKRIYERAVGSLMAPAVFNNTKITLDSNGNIYTMDGSIQV
FDGHLKVTGDKSKDKFLPEYKIGDKLNADLVEKIEKQTQPPARFNEASLIKALEANGIGRPSTYASIIKTIKARDYVIVD
EKRFIPTEQGKLTVDTLNQYFNKIMKIDYTSNMESELDLIADGKVDHIQLLKDFYNKFMPILEAAQQNMEKIKPVVTDKI
CPLCGGNLVIRKSRYGEFYGCSNFPKCKHIEQMSE
>Mature_594_residues
SDKVIIVESPAKSKTISSYFNDEVTVLSSVGHIRDLATSGKGGLGVDVDNNFEPTYKIITGKGKLITDLKKKTKGKEVLI
ATDPDREGEAIAWHIADELGLDLNEHNRIVFKEITKPAVMEAINHKRQIDLGLVNSQESRRILDRIIGFKLSAFLRDKIK
SRSAGRVQSVALKLIVDLEREIEKFVPETYFTMHAHFNDHVADYVIPKDTRIGLEEATKIVETSTNPFLVSDITQKETKR
NAKPPYMTSTLLQDGVNSLYMSSSRVMSVASELYQGIEINGQIIGLITYMKTDSNRLSKEFVNPAKAFIETNYGKEYVGF
YKTNNKDSSQDAHEAIRPTDIHLTPEKIEQYLSKDQFKLYKRIYERAVGSLMAPAVFNNTKITLDSNGNIYTMDGSIQVF
DGHLKVTGDKSKDKFLPEYKIGDKLNADLVEKIEKQTQPPARFNEASLIKALEANGIGRPSTYASIIKTIKARDYVIVDE
KRFIPTEQGKLTVDTLNQYFNKIMKIDYTSNMESELDLIADGKVDHIQLLKDFYNKFMPILEAAQQNMEKIKPVVTDKIC
PLCGGNLVIRKSRYGEFYGCSNFPKCKHIEQMSE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=587, Percent_Identity=25.7240204429302, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI4507635, Length=479, Percent_Identity=24.0083507306889, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1787529, Length=641, Percent_Identity=37.2854914196568, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32563869, Length=555, Percent_Identity=24.3243243243243, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17555378, Length=534, Percent_Identity=26.7790262172285, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24585251, Length=658, Percent_Identity=25.0759878419453, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640096, Length=508, Percent_Identity=23.0314960629921, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 67206; Mature: 67075

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKVIIVESPAKSKTISSYFNDEVTVLSSVGHIRDLATSGKGGLGVDVDNNFEPTYKII
CCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEE
TGKGKLITDLKKKTKGKEVLIATDPDREGEAIAWHIADELGLDLNEHNRIVFKEITKPAV
ECCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEHHHHCCCCCCCCEEEEHHHCCHHH
MEAINHKRQIDLGLVNSQESRRILDRIIGFKLSAFLRDKIKSRSAGRVQSVALKLIVDLE
HHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
REIEKFVPETYFTMHAHFNDHVADYVIPKDTRIGLEEATKIVETSTNPFLVSDITQKETK
HHHHHHCCCCEEEEEECCCCCHHHEECCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHH
RNAKPPYMTSTLLQDGVNSLYMSSSRVMSVASELYQGIEINGQIIGLITYMKTDSNRLSK
CCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCEECCCEEEEEEEEEECCCHHHHH
EFVNPAKAFIETNYGKEYVGFYKTNNKDSSQDAHEAIRPTDIHLTPEKIEQYLSKDQFKL
HHHCHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCEEECHHHHHHHHCHHHHHH
YKRIYERAVGSLMAPAVFNNTKITLDSNGNIYTMDGSIQVFDGHLKVTGDKSKDKFLPEY
HHHHHHHHHHHHHCCCEECCCEEEECCCCCEEEECCEEEEEECEEEEECCCCCCCCCCCC
KIGDKLNADLVEKIEKQTQPPARFNEASLIKALEANGIGRPSTYASIIKTIKARDYVIVD
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEC
EKRFIPTEQGKLTVDTLNQYFNKIMKIDYTSNMESELDLIADGKVDHIQLLKDFYNKFMP
CCCCCCCCCCCEEHHHHHHHHHHHEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
ILEAAQQNMEKIKPVVTDKICPLCGGNLVIRKSRYGEFYGCSNFPKCKHIEQMSE
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SDKVIIVESPAKSKTISSYFNDEVTVLSSVGHIRDLATSGKGGLGVDVDNNFEPTYKII
CCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEE
TGKGKLITDLKKKTKGKEVLIATDPDREGEAIAWHIADELGLDLNEHNRIVFKEITKPAV
ECCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEHHHHCCCCCCCCEEEEHHHCCHHH
MEAINHKRQIDLGLVNSQESRRILDRIIGFKLSAFLRDKIKSRSAGRVQSVALKLIVDLE
HHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
REIEKFVPETYFTMHAHFNDHVADYVIPKDTRIGLEEATKIVETSTNPFLVSDITQKETK
HHHHHHCCCCEEEEEECCCCCHHHEECCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHH
RNAKPPYMTSTLLQDGVNSLYMSSSRVMSVASELYQGIEINGQIIGLITYMKTDSNRLSK
CCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCEECCCEEEEEEEEEECCCHHHHH
EFVNPAKAFIETNYGKEYVGFYKTNNKDSSQDAHEAIRPTDIHLTPEKIEQYLSKDQFKL
HHHCHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCEEECHHHHHHHHCHHHHHH
YKRIYERAVGSLMAPAVFNNTKITLDSNGNIYTMDGSIQVFDGHLKVTGDKSKDKFLPEY
HHHHHHHHHHHHHCCCEECCCEEEECCCCCEEEECCEEEEEECEEEEECCCCCCCCCCCC
KIGDKLNADLVEKIEKQTQPPARFNEASLIKALEANGIGRPSTYASIIKTIKARDYVIVD
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEC
EKRFIPTEQGKLTVDTLNQYFNKIMKIDYTSNMESELDLIADGKVDHIQLLKDFYNKFMP
CCCCCCCCCCCEEHHHHHHHHHHHEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
ILEAAQQNMEKIKPVVTDKICPLCGGNLVIRKSRYGEFYGCSNFPKCKHIEQMSE
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]