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The map label for this gene is rnhB

Identifier: 162447204

GI number: 162447204

Start: 350249

End: 350857

Strand: Direct

Name: rnhB

Synonym: ACL_0338

Alternate gene names: 162447204

Gene position: 350249-350857 (Clockwise)

Preceding gene: 162447203

Following gene: 162447205

Centisome position: 23.4

GC content: 33.66

Gene sequence:

>609_bases
TTGACCATAGACAATCTAGCATTTGAAAAAGCACTTTATGATAAAGGGTATACCTATATTTGTGGTGTAGATGAAGCTGG
ACGTGGTCCACTAGCTGGTCCAGTTGTTGCTGCAGCTGTAATTTTCGAAAAAGGTTTTTATCATGAAGACATTAATGATT
CTAAAACTTTATCAGCTAAAAAACGTGAAACCTTATTTGAACTCATTAAACAACATGCACTTGCAATTGGTGTAAGTGTT
GTAAATCATGAAGTGATTGATAAAATAAATATATTAGAAGCAGCAAGACATGCAATGGAAGATGCTATTTCAAAACTAAA
GATTAAACCACAATACGCATTAACTGATCATATGGATATAAAAGGTATACCTTATACCTCCATTAAACATGGCGACCAAC
TATCTATATCGATTGCTGCAGCAAGTATAATTGCTAAAGTGACTAGAGATAAATTGATGGTGGATTATGATGAGGTTTAT
CCTCAATACGGATTTAAAGACCATAAAGGTTATGGTACAAGAAAACATTTAGAGGCTCTAAATTTGTATGGTGTATCACC
AATTCACCGTAAAACTTTTGCGCCCGTGGCTAAGTTATTAAAACAATAA

Upstream 100 bases:

>100_bases
GTAAGAGGTGCTATTGTTAAAGGTGGCGAAACTGATTATGACAGAGTATATACGATATTATTAACAGATATAAGAAATAG
AAATTTAGGAGCACTAAGCT

Downstream 100 bases:

>100_bases
TCATGTGAAATCATGGTTTTGTTTTTCTATATGGACTAAAAAATGTAAAAAAAACCTTCTATATAATATATTTATATAGA
ATAAAATATAAGAAAATTGA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MTIDNLAFEKALYDKGYTYICGVDEAGRGPLAGPVVAAAVIFEKGFYHEDINDSKTLSAKKRETLFELIKQHALAIGVSV
VNHEVIDKINILEAARHAMEDAISKLKIKPQYALTDHMDIKGIPYTSIKHGDQLSISIAAASIIAKVTRDKLMVDYDEVY
PQYGFKDHKGYGTRKHLEALNLYGVSPIHRKTFAPVAKLLKQ

Sequences:

>Translated_202_residues
MTIDNLAFEKALYDKGYTYICGVDEAGRGPLAGPVVAAAVIFEKGFYHEDINDSKTLSAKKRETLFELIKQHALAIGVSV
VNHEVIDKINILEAARHAMEDAISKLKIKPQYALTDHMDIKGIPYTSIKHGDQLSISIAAASIIAKVTRDKLMVDYDEVY
PQYGFKDHKGYGTRKHLEALNLYGVSPIHRKTFAPVAKLLKQ
>Mature_201_residues
TIDNLAFEKALYDKGYTYICGVDEAGRGPLAGPVVAAAVIFEKGFYHEDINDSKTLSAKKRETLFELIKQHALAIGVSVV
NHEVIDKINILEAARHAMEDAISKLKIKPQYALTDHMDIKGIPYTSIKHGDQLSISIAAASIIAKVTRDKLMVDYDEVYP
QYGFKDHKGYGTRKHLEALNLYGVSPIHRKTFAPVAKLLKQ

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=183, Percent_Identity=45.3551912568306, Blast_Score=148, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_ACHLI (A9NF30)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620336.1
- ProteinModelPortal:   A9NF30
- SMR:   A9NF30
- GeneID:   5804320
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0338
- HOGENOM:   HBG584843
- OMA:   IICGVDE
- BioCyc:   ALAI441768:ACL_0338-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 22471; Mature: 22340

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDNLAFEKALYDKGYTYICGVDEAGRGPLAGPVVAAAVIFEKGFYHEDINDSKTLSAK
CCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHH
KRETLFELIKQHALAIGVSVVNHEVIDKINILEAARHAMEDAISKLKIKPQYALTDHMDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC
KGIPYTSIKHGDQLSISIAAASIIAKVTRDKLMVDYDEVYPQYGFKDHKGYGTRKHLEAL
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHEECHHHHCCCCCCCCCCCCCHHHHHHHH
NLYGVSPIHRKTFAPVAKLLKQ
HHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIDNLAFEKALYDKGYTYICGVDEAGRGPLAGPVVAAAVIFEKGFYHEDINDSKTLSAK
CCCHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHH
KRETLFELIKQHALAIGVSVVNHEVIDKINILEAARHAMEDAISKLKIKPQYALTDHMDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC
KGIPYTSIKHGDQLSISIAAASIIAKVTRDKLMVDYDEVYPQYGFKDHKGYGTRKHLEAL
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHEECHHHHCCCCCCCCCCCCCHHHHHHHH
NLYGVSPIHRKTFAPVAKLLKQ
HHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA