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The map label for this gene is fusA [H]

Identifier: 162447164

GI number: 162447164

Start: 307936

End: 309993

Strand: Direct

Name: fusA [H]

Synonym: ACL_0298

Alternate gene names: 162447164

Gene position: 307936-309993 (Clockwise)

Preceding gene: 162447162

Following gene: 162447165

Centisome position: 20.57

GC content: 35.18

Gene sequence:

>2058_bases
ATGAAAGAATACAAAACCAAGAACCTCAGAAACATTGCGCTATTAGGGCATTTGGGTAGTGGTAAGACTTCACTAACTGA
AAGCCTCTTAAATGTAACTGGCGTAACTAACGTAAAAGGTGAGGTGGAACGTAAAAACACCAAATCCGATTTTCTTGTGG
AGGAACAAACACGTATTGCCTCCATGCAAACATCTTTAATACCGATCGAAGCACATGACTGCAAACTTAACTTTCTAGAC
GTACCAGGTAATGATGAGCTCATTAGCGAATTATACCATGCCCTAGAAGTAGTTAAGGGTGCAGTCATTTTAATTGATGC
GACCAAAGGGGTTGAAGTTGGCACGGAACGTGTTTGGAATGAAATTAGACGTAGACGTATACCAGCAGTATTATTTATAA
ATAAAATGGATAAAGATAATATTGACTATGAAGCATTATTAGAAGATATACGTACGAAACTTGGTAAACGTGCCGTACCT
TTTACATATCCTATAGGACGTAAAGAAGATTTTGAAGGCTTTGTAAATGTTGTTGAGATGAAGGCTCGTATCTATAACGG
TAATACTTGCGAAGATGCAGAAATTTGGGATGAAAAGAAACCTAAAGTGGAACAACTTCGTGAGATGATTATGGAATCAG
TAGCTGAAACATCCGAAGAATTATTAGAAAAATACTTTGGTGGTGAAGAAATCACTAATGAAGAAATCACACATGCTTTA
AATCACGCAATTATTAATGGTGAGTTAACACCAGTCTTAGTAGGTTCTGTACTAAAAAATATTGGTGTAAATACCTTACT
TAAAATGTTAACTGACTTCTTACCTTCTCCTGATATGTTAAGAGAATTAGAAGCAACTAAAGTTGGAAGTGATGAAAAAC
TTCAAGTTAAAACATTGGATGAAGAACCATTTTCAGGATTTGTCTTTAAAACAATGATTGACCCATTTGTTGGAACCATT
TCATTTATTAAATTAAATTCAGGTATCTTAAAAGTTGGGGATGAAGTATATCACGCACAAAGTGATTCAACCGTTAAGAT
TAATACCTTAATGACACTGATTGGTAAAGATCAAATACCAATGGATTTAGCACATGCGGGTGATATTGTAGTTACAACTA
AATTAGATGCATTAAAGACTGGTCATACAATCACAGATACTAAACGCAAAATTGTATATGATACAGTGAAACTTCCTACA
CCAGTTATCTATCAGGCAATTGAACCAGATAATGAAAAAGATGAAGACAAGATTTCAATGGCTTTAACAAAACTAAATAT
TGAAGATCCTTCATTTGATATCCAAAGAAATAAAGAAACTGGTCAATTACTCATTGGTGGTTATGGTATTAGTCATATTA
AATACATTACAGACCGTATGAAGAATCTATATAAGGTCAGTGTTTCATTCGAAGACCAAAAGATAGTATATAGAGAAACG
ATTAAGAAAAAAGGTGTTGGCGAAGGTAAACATAAGAAACAATCCGGTGGTGCAGGTCAATTTGGTCATGTTTGGATACG
TTTTGAACCAACAGATAAGCTTTTCGAGTTTTCTGAAGAAGTATTTGGGGGTGCAGTACCTAGATCATACTTCCCAGCCG
TTGAAAAAGGATTAATTCGTGCTTTTGAAAAAGGTCCTTTGGCAGGGTTCCCTGTAATTCATGTGAAAGCAACCCTTTAT
GATGGAAGTTATCACTCAGTAGATAGTAATGAAATATCGTTTGTTATGGCAGCTGATCTCGCATTTAAAGCGGCATTACC
TGAAATTCAACCAACGATATTAGAGCCAATTTTACACCTAACAATCACTGTTAAAGAATCTTATGTTGGTGATGTTATGG
GGGATATGAATAAACGTCGTGGTCGTATACTAGGTATGGATCAAGCAGATGGTTACACGGTAATTAATGCATATGTTCCA
GAAGCAGAAGTCACTACTTACGCCATAGATTTAAAAGCAATGACTCAAGGTTCAGGACAGTTTGAAAGACGTTTTGATCG
TTACGAAGAAGTACCACAACAATTCCAATCCAAGATTATTGAACAATATAAAAAGTAA

Upstream 100 bases:

>100_bases
AAAATTCACATATATTTATTTATATTAAATATTGATAAAAAAATAAGGTGTGATATAATAAAAGTACAATGTAAACGCAT
ACATATATAAGGAGGTATTT

Downstream 100 bases:

>100_bases
AAACATATCATTTTAACTACTATAGTATGGAGGAATTAGTCCATGCAGTGTAAAATATGTAGAAATGATATAAAGGTTCA
AAGAACTTTTAGAAATTTAC

Product: translation elongation factor EF-G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 685; Mature: 685

Protein sequence:

>685_residues
MKEYKTKNLRNIALLGHLGSGKTSLTESLLNVTGVTNVKGEVERKNTKSDFLVEEQTRIASMQTSLIPIEAHDCKLNFLD
VPGNDELISELYHALEVVKGAVILIDATKGVEVGTERVWNEIRRRRIPAVLFINKMDKDNIDYEALLEDIRTKLGKRAVP
FTYPIGRKEDFEGFVNVVEMKARIYNGNTCEDAEIWDEKKPKVEQLREMIMESVAETSEELLEKYFGGEEITNEEITHAL
NHAIINGELTPVLVGSVLKNIGVNTLLKMLTDFLPSPDMLRELEATKVGSDEKLQVKTLDEEPFSGFVFKTMIDPFVGTI
SFIKLNSGILKVGDEVYHAQSDSTVKINTLMTLIGKDQIPMDLAHAGDIVVTTKLDALKTGHTITDTKRKIVYDTVKLPT
PVIYQAIEPDNEKDEDKISMALTKLNIEDPSFDIQRNKETGQLLIGGYGISHIKYITDRMKNLYKVSVSFEDQKIVYRET
IKKKGVGEGKHKKQSGGAGQFGHVWIRFEPTDKLFEFSEEVFGGAVPRSYFPAVEKGLIRAFEKGPLAGFPVIHVKATLY
DGSYHSVDSNEISFVMAADLAFKAALPEIQPTILEPILHLTITVKESYVGDVMGDMNKRRGRILGMDQADGYTVINAYVP
EAEVTTYAIDLKAMTQGSGQFERRFDRYEEVPQQFQSKIIEQYKK

Sequences:

>Translated_685_residues
MKEYKTKNLRNIALLGHLGSGKTSLTESLLNVTGVTNVKGEVERKNTKSDFLVEEQTRIASMQTSLIPIEAHDCKLNFLD
VPGNDELISELYHALEVVKGAVILIDATKGVEVGTERVWNEIRRRRIPAVLFINKMDKDNIDYEALLEDIRTKLGKRAVP
FTYPIGRKEDFEGFVNVVEMKARIYNGNTCEDAEIWDEKKPKVEQLREMIMESVAETSEELLEKYFGGEEITNEEITHAL
NHAIINGELTPVLVGSVLKNIGVNTLLKMLTDFLPSPDMLRELEATKVGSDEKLQVKTLDEEPFSGFVFKTMIDPFVGTI
SFIKLNSGILKVGDEVYHAQSDSTVKINTLMTLIGKDQIPMDLAHAGDIVVTTKLDALKTGHTITDTKRKIVYDTVKLPT
PVIYQAIEPDNEKDEDKISMALTKLNIEDPSFDIQRNKETGQLLIGGYGISHIKYITDRMKNLYKVSVSFEDQKIVYRET
IKKKGVGEGKHKKQSGGAGQFGHVWIRFEPTDKLFEFSEEVFGGAVPRSYFPAVEKGLIRAFEKGPLAGFPVIHVKATLY
DGSYHSVDSNEISFVMAADLAFKAALPEIQPTILEPILHLTITVKESYVGDVMGDMNKRRGRILGMDQADGYTVINAYVP
EAEVTTYAIDLKAMTQGSGQFERRFDRYEEVPQQFQSKIIEQYKK
>Mature_685_residues
MKEYKTKNLRNIALLGHLGSGKTSLTESLLNVTGVTNVKGEVERKNTKSDFLVEEQTRIASMQTSLIPIEAHDCKLNFLD
VPGNDELISELYHALEVVKGAVILIDATKGVEVGTERVWNEIRRRRIPAVLFINKMDKDNIDYEALLEDIRTKLGKRAVP
FTYPIGRKEDFEGFVNVVEMKARIYNGNTCEDAEIWDEKKPKVEQLREMIMESVAETSEELLEKYFGGEEITNEEITHAL
NHAIINGELTPVLVGSVLKNIGVNTLLKMLTDFLPSPDMLRELEATKVGSDEKLQVKTLDEEPFSGFVFKTMIDPFVGTI
SFIKLNSGILKVGDEVYHAQSDSTVKINTLMTLIGKDQIPMDLAHAGDIVVTTKLDALKTGHTITDTKRKIVYDTVKLPT
PVIYQAIEPDNEKDEDKISMALTKLNIEDPSFDIQRNKETGQLLIGGYGISHIKYITDRMKNLYKVSVSFEDQKIVYRET
IKKKGVGEGKHKKQSGGAGQFGHVWIRFEPTDKLFEFSEEVFGGAVPRSYFPAVEKGLIRAFEKGPLAGFPVIHVKATLY
DGSYHSVDSNEISFVMAADLAFKAALPEIQPTILEPILHLTITVKESYVGDVMGDMNKRRGRILGMDQADGYTVINAYVP
EAEVTTYAIDLKAMTQGSGQFERRFDRYEEVPQQFQSKIIEQYKK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=692, Percent_Identity=31.5028901734104, Blast_Score=358, Evalue=1e-98,
Organism=Homo sapiens, GI19923640, Length=721, Percent_Identity=26.9070735090153, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI25306283, Length=441, Percent_Identity=27.2108843537415, Blast_Score=167, Evalue=4e-41,
Organism=Homo sapiens, GI25306287, Length=286, Percent_Identity=31.4685314685315, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI217272894, Length=789, Percent_Identity=20.9125475285171, Blast_Score=85, Evalue=3e-16,
Organism=Homo sapiens, GI217272892, Length=789, Percent_Identity=20.9125475285171, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI4503483, Length=152, Percent_Identity=32.2368421052632, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=30.9352517985612, Blast_Score=74, Evalue=7e-13,
Organism=Escherichia coli, GI1789738, Length=696, Percent_Identity=35.7758620689655, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1790835, Length=499, Percent_Identity=26.25250501002, Blast_Score=169, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17533571, Length=693, Percent_Identity=30.3030303030303, Blast_Score=345, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI17556745, Length=712, Percent_Identity=23.876404494382, Blast_Score=197, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17552882, Length=135, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=695, Percent_Identity=30.0719424460432, Blast_Score=338, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6322359, Length=776, Percent_Identity=25.7731958762887, Blast_Score=220, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6324707, Length=164, Percent_Identity=29.8780487804878, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=164, Percent_Identity=29.8780487804878, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24582462, Length=692, Percent_Identity=32.2254335260116, Blast_Score=357, Evalue=2e-98,
Organism=Drosophila melanogaster, GI221458488, Length=716, Percent_Identity=25.8379888268156, Blast_Score=228, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24585709, Length=802, Percent_Identity=21.9451371571072, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24585711, Length=802, Percent_Identity=21.9451371571072, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24585713, Length=802, Percent_Identity=21.9451371571072, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 76981; Mature: 76981

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEYKTKNLRNIALLGHLGSGKTSLTESLLNVTGVTNVKGEVERKNTKSDFLVEEQTRIA
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCEEHHHHHHH
SMQTSLIPIEAHDCKLNFLDVPGNDELISELYHALEVVKGAVILIDATKGVEVGTERVWN
HHHHHEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHH
EIRRRRIPAVLFINKMDKDNIDYEALLEDIRTKLGKRAVPFTYPIGRKEDFEGFVNVVEM
HHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHH
KARIYNGNTCEDAEIWDEKKPKVEQLREMIMESVAETSEELLEKYFGGEEITNEEITHAL
HHHEECCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
NHAIINGELTPVLVGSVLKNIGVNTLLKMLTDFLPSPDMLRELEATKVGSDEKLQVKTLD
HHHEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECC
EEPFSGFVFKTMIDPFVGTISFIKLNSGILKVGDEVYHAQSDSTVKINTLMTLIGKDQIP
CCCCCCHHHHHHHHHHHHHEEEEEECCCHHHCCCHHHCCCCCCEEEHHHHHHHHCCCCCC
MDLAHAGDIVVTTKLDALKTGHTITDTKRKIVYDTVKLPTPVIYQAIEPDNEKDEDKISM
CHHHCCCCEEEEEEHHHHHCCCCCCCCCCEEEEEECCCCCHHHEEECCCCCCCCHHHHHE
ALTKLNIEDPSFDIQRNKETGQLLIGGYGISHIKYITDRMKNLYKVSVSFEDQKIVYRET
EHEEECCCCCCCCEECCCCCCCEEEECCCHHHHHHHHHHHHCEEEEEEECCCCHHHHHHH
IKKKGVGEGKHKKQSGGAGQFGHVWIRFEPTDKLFEFSEEVFGGAVPRSYFPAVEKGLIR
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AFEKGPLAGFPVIHVKATLYDGSYHSVDSNEISFVMAADLAFKAALPEIQPTILEPILHL
HHHCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEEEHHHHHHHCCCCCHHHHHHHHEE
TITVKESYVGDVMGDMNKRRGRILGMDQADGYTVINAYVPEAEVTTYAIDLKAMTQGSGQ
EEEEEHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCCH
FERRFDRYEEVPQQFQSKIIEQYKK
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEYKTKNLRNIALLGHLGSGKTSLTESLLNVTGVTNVKGEVERKNTKSDFLVEEQTRIA
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCEEHHHHHHH
SMQTSLIPIEAHDCKLNFLDVPGNDELISELYHALEVVKGAVILIDATKGVEVGTERVWN
HHHHHEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHH
EIRRRRIPAVLFINKMDKDNIDYEALLEDIRTKLGKRAVPFTYPIGRKEDFEGFVNVVEM
HHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHH
KARIYNGNTCEDAEIWDEKKPKVEQLREMIMESVAETSEELLEKYFGGEEITNEEITHAL
HHHEECCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
NHAIINGELTPVLVGSVLKNIGVNTLLKMLTDFLPSPDMLRELEATKVGSDEKLQVKTLD
HHHEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECC
EEPFSGFVFKTMIDPFVGTISFIKLNSGILKVGDEVYHAQSDSTVKINTLMTLIGKDQIP
CCCCCCHHHHHHHHHHHHHEEEEEECCCHHHCCCHHHCCCCCCEEEHHHHHHHHCCCCCC
MDLAHAGDIVVTTKLDALKTGHTITDTKRKIVYDTVKLPTPVIYQAIEPDNEKDEDKISM
CHHHCCCCEEEEEEHHHHHCCCCCCCCCCEEEEEECCCCCHHHEEECCCCCCCCHHHHHE
ALTKLNIEDPSFDIQRNKETGQLLIGGYGISHIKYITDRMKNLYKVSVSFEDQKIVYRET
EHEEECCCCCCCCEECCCCCCCEEEECCCHHHHHHHHHHHHCEEEEEEECCCCHHHHHHH
IKKKGVGEGKHKKQSGGAGQFGHVWIRFEPTDKLFEFSEEVFGGAVPRSYFPAVEKGLIR
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AFEKGPLAGFPVIHVKATLYDGSYHSVDSNEISFVMAADLAFKAALPEIQPTILEPILHL
HHHCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEEEHHHHHHHCCCCCHHHHHHHHEE
TITVKESYVGDVMGDMNKRRGRILGMDQADGYTVINAYVPEAEVTTYAIDLKAMTQGSGQ
EEEEEHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCCH
FERRFDRYEEVPQQFQSKIIEQYKK
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]