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The map label for this gene is proS [H]

Identifier: 162447162

GI number: 162447162

Start: 305325

End: 306749

Strand: Direct

Name: proS [H]

Synonym: ACL_0296

Alternate gene names: 162447162

Gene position: 305325-306749 (Clockwise)

Preceding gene: 162447161

Following gene: 162447164

Centisome position: 20.4

GC content: 34.53

Gene sequence:

>1425_bases
ATGAGTAAAAATAAAAAATTAGTTGAAAGCATTACATCAAGAGATGTAGACTTTAGTCAATGGTATACAGACCTTTGTCT
AAAAGCAGAGTTAATGGATTATTCTAACACACAAGGGTTTATTATCTATAGACCTTTAGGATATGGTATTTGGGAAAATA
TCCAAAACTACCTAAATAATGAATTTAGAAAAACAGGGCATCAAAACGTATATATGCCCATGTTGATTCCAGAAAGTTTA
TTCCAAAAAGAAAAGGATCACGTTTCAGGTTTTGCACCTGAAACAGCGATGGTTACAACAACAGGGGTTGAAAACTTAAG
CGAGCGCTTAATTATTCGCCCTACATCAGAAGTATTATTTGGTACACATTATAGTAAAATTGTTAAATCCTATAGAGATT
TACCAAAAATGTATAACCAATGGTGTTCTGTTGTTCGCTGGGAGAAGACTACACGCCCATTTTTACGCGGTAAAGAGTTT
TTATGGCAAGAAGGTCACACAGTTCATGCGACTGCTGAAGAGGCTAAAAAAGAGACACATCAAATGTTAGAAGTGTATGA
CAAATTAGGTAAAGAGTTACTAGCTATACCATTTGTTACAGGACGTAAGACAGATAAAGAAAAATTTGCTGGTGCACTTG
AAACTTATGCGATTGAAGCATTAATGCATGATGGACAAGCACTACAATCTGGTACATCACACTATTTTGGTAATGAGTTT
GCTCATGCCTTTGATATCAAATTCTTAAATGATAAAAATGAACTAGAATATGCATACCAAACTTCTTGGGGTGTATCTAC
AAGACTTATTGGCGCTATCATTATGGTACATGGTGATGACAATGGTTTAGTATTACCGCCATATGTTGCACCAACTCAAG
TGGTCATTGTACCAATTAGACCAAATGAAGAAGTGCTAAGTGCTGTTAAACAAATTGAAAATGAACTATCAGATAAATTT
AGAGTATTTGTAGATGATTCTGATAAATCACCTGGATGGAAATTTAGTGAACACGAAATGAAGGGTGTACCACTACGTAT
CGAAATTGGTCCAAGAGATTTAGAAAACGGTCAAGTTGTTGTAACATTTAGACATAACTATGAAAAACATACAGTAGCAC
TTACTGATTTACATAAGTTTGTTGAAGATGGTTTAAAAACAATGCATGAAGCAATGTATCAAAAAGCATTAGAAGCTACA
ACAAGTAGAACTTATGAAGCGAAAAGCTATGACGAATTTAAAGCATTCTTAAAACAGGGCGGATATATTAAAATGTCAAC
TTGTGGCGAAGAAGCTGAAATCCAAATTAAGGAAGAAACAGGTGCTACAGCACGTGTGATCCTAGATGAACCACTACTTA
CAGAAAATTGTCCAGTAACTGGTAAAAAAGCTACTCAAACAATTCTTTTTGCAAGAGCATACTAA

Upstream 100 bases:

>100_bases
TTTGGACTAAGTAGAAAATCATTTAAACGTGCATTAGGTTTATTGTATAAAGAAAGAAAAGTAAAATTTGAAGGAAATGA
AACAATTTTGGTGAAATCAA

Downstream 100 bases:

>100_bases
TAACTATAAAAAGAAGCAGAAATATCAAATTAGATATTTCTGCTTTTATTTTGTTTAACGCATGAATTGTACAAACTCAG
ACAACTGAGATGCCCAAAGT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSKNKKLVESITSRDVDFSQWYTDLCLKAELMDYSNTQGFIIYRPLGYGIWENIQNYLNNEFRKTGHQNVYMPMLIPESL
FQKEKDHVSGFAPETAMVTTTGVENLSERLIIRPTSEVLFGTHYSKIVKSYRDLPKMYNQWCSVVRWEKTTRPFLRGKEF
LWQEGHTVHATAEEAKKETHQMLEVYDKLGKELLAIPFVTGRKTDKEKFAGALETYAIEALMHDGQALQSGTSHYFGNEF
AHAFDIKFLNDKNELEYAYQTSWGVSTRLIGAIIMVHGDDNGLVLPPYVAPTQVVIVPIRPNEEVLSAVKQIENELSDKF
RVFVDDSDKSPGWKFSEHEMKGVPLRIEIGPRDLENGQVVVTFRHNYEKHTVALTDLHKFVEDGLKTMHEAMYQKALEAT
TSRTYEAKSYDEFKAFLKQGGYIKMSTCGEEAEIQIKEETGATARVILDEPLLTENCPVTGKKATQTILFARAY

Sequences:

>Translated_474_residues
MSKNKKLVESITSRDVDFSQWYTDLCLKAELMDYSNTQGFIIYRPLGYGIWENIQNYLNNEFRKTGHQNVYMPMLIPESL
FQKEKDHVSGFAPETAMVTTTGVENLSERLIIRPTSEVLFGTHYSKIVKSYRDLPKMYNQWCSVVRWEKTTRPFLRGKEF
LWQEGHTVHATAEEAKKETHQMLEVYDKLGKELLAIPFVTGRKTDKEKFAGALETYAIEALMHDGQALQSGTSHYFGNEF
AHAFDIKFLNDKNELEYAYQTSWGVSTRLIGAIIMVHGDDNGLVLPPYVAPTQVVIVPIRPNEEVLSAVKQIENELSDKF
RVFVDDSDKSPGWKFSEHEMKGVPLRIEIGPRDLENGQVVVTFRHNYEKHTVALTDLHKFVEDGLKTMHEAMYQKALEAT
TSRTYEAKSYDEFKAFLKQGGYIKMSTCGEEAEIQIKEETGATARVILDEPLLTENCPVTGKKATQTILFARAY
>Mature_473_residues
SKNKKLVESITSRDVDFSQWYTDLCLKAELMDYSNTQGFIIYRPLGYGIWENIQNYLNNEFRKTGHQNVYMPMLIPESLF
QKEKDHVSGFAPETAMVTTTGVENLSERLIIRPTSEVLFGTHYSKIVKSYRDLPKMYNQWCSVVRWEKTTRPFLRGKEFL
WQEGHTVHATAEEAKKETHQMLEVYDKLGKELLAIPFVTGRKTDKEKFAGALETYAIEALMHDGQALQSGTSHYFGNEFA
HAFDIKFLNDKNELEYAYQTSWGVSTRLIGAIIMVHGDDNGLVLPPYVAPTQVVIVPIRPNEEVLSAVKQIENELSDKFR
VFVDDSDKSPGWKFSEHEMKGVPLRIEIGPRDLENGQVVVTFRHNYEKHTVALTDLHKFVEDGLKTMHEAMYQKALEATT
SRTYEAKSYDEFKAFLKQGGYIKMSTCGEEAEIQIKEETGATARVILDEPLLTENCPVTGKKATQTILFARAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=493, Percent_Identity=41.5821501014199, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI1786392, Length=167, Percent_Identity=24.5508982035928, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71991342, Length=498, Percent_Identity=40.3614457831325, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71991346, Length=367, Percent_Identity=43.3242506811989, Blast_Score=325, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6321808, Length=509, Percent_Identity=39.2927308447937, Blast_Score=366, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24649468, Length=495, Percent_Identity=40, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI24649466, Length=495, Percent_Identity=40, Blast_Score=354, Evalue=9e-98,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 54253; Mature: 54122

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNKKLVESITSRDVDFSQWYTDLCLKAELMDYSNTQGFIIYRPLGYGIWENIQNYLNN
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHH
EFRKTGHQNVYMPMLIPESLFQKEKDHVSGFAPETAMVTTTGVENLSERLIIRPTSEVLF
HHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCCEEEECCHHHHH
GTHYSKIVKSYRDLPKMYNQWCSVVRWEKTTRPFLRGKEFLWQEGHTVHATAEEAKKETH
HHHHHHHHHHHHHHHHHHHHHHHHEECCHHCCHHHCCCHHHHCCCCEEEECHHHHHHHHH
QMLEVYDKLGKELLAIPFVTGRKTDKEKFAGALETYAIEALMHDGQALQSGTSHYFGNEF
HHHHHHHHHCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCC
AHAFDIKFLNDKNELEYAYQTSWGVSTRLIGAIIMVHGDDNGLVLPPYVAPTQVVIVPIR
CEEEEEEEECCCCCEEEEEECCCCHHHEEEEEEEEEEECCCCEEECCCCCCCEEEEEEEC
PNEEVLSAVKQIENELSDKFRVFVDDSDKSPGWKFSEHEMKGVPLRIEIGPRDLENGQVV
CCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEE
VTFRHNYEKHTVALTDLHKFVEDGLKTMHEAMYQKALEATTSRTYEAKSYDEFKAFLKQG
EEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
GYIKMSTCGEEAEIQIKEETGATARVILDEPLLTENCPVTGKKATQTILFARAY
CEEEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHEEEEEEECC
>Mature Secondary Structure 
SKNKKLVESITSRDVDFSQWYTDLCLKAELMDYSNTQGFIIYRPLGYGIWENIQNYLNN
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHH
EFRKTGHQNVYMPMLIPESLFQKEKDHVSGFAPETAMVTTTGVENLSERLIIRPTSEVLF
HHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCCEEEECCHHHHH
GTHYSKIVKSYRDLPKMYNQWCSVVRWEKTTRPFLRGKEFLWQEGHTVHATAEEAKKETH
HHHHHHHHHHHHHHHHHHHHHHHHEECCHHCCHHHCCCHHHHCCCCEEEECHHHHHHHHH
QMLEVYDKLGKELLAIPFVTGRKTDKEKFAGALETYAIEALMHDGQALQSGTSHYFGNEF
HHHHHHHHHCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCC
AHAFDIKFLNDKNELEYAYQTSWGVSTRLIGAIIMVHGDDNGLVLPPYVAPTQVVIVPIR
CEEEEEEEECCCCCEEEEEECCCCHHHEEEEEEEEEEECCCCEEECCCCCCCEEEEEEEC
PNEEVLSAVKQIENELSDKFRVFVDDSDKSPGWKFSEHEMKGVPLRIEIGPRDLENGQVV
CCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEE
VTFRHNYEKHTVALTDLHKFVEDGLKTMHEAMYQKALEATTSRTYEAKSYDEFKAFLKQG
EEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
GYIKMSTCGEEAEIQIKEETGATARVILDEPLLTENCPVTGKKATQTILFARAY
CEEEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA