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The map label for this gene is rlmN

Identifier: 162447121

GI number: 162447121

Start: 251471

End: 252487

Strand: Direct

Name: rlmN

Synonym: ACL_0251

Alternate gene names: 162447121

Gene position: 251471-252487 (Clockwise)

Preceding gene: 162447120

Following gene: 162447122

Centisome position: 16.8

GC content: 31.56

Gene sequence:

>1017_bases
ATGCTAATTTATGATTTAACATACGAAGAATTAGAAGAATTCATCGTAGAAAACGGCTATAAGAAATTTAGAGCCGATCA
AATTTGGAACTGGCTCTATAAACAAAAAATAGAGGCGTTCAGTGAAATGAATAACATCCCAGAGGACATCATTAAATTAT
TGAATGATAATTATACCTTTGCAGGACTTGAAACAGTGATCAAAAATACCAGTGCAGATGGTACAATTAAGTTTTTATTT
GACTTAAAAGATGCGAATCTAATTGAAACGGTATTAATGAGTCATAACTATGGCATGAGTGCCTGTGTGACTACTCAAGT
AGGATGTAACATTGGATGTAGTTTTTGTGCATCTGGTGTATTAAAGAAAAAAAGAGACTTAACAGCAGGTGAAATTGTTG
CACAAATCATTCGTGCAGAAAAAGAATCTGGTGTGAGAGTGTCTAGTATTGTTATTATGGGTATTGGTGAACCATTTGAT
AACTATAAGAACTTTGTTAAATTTATTAGCATTGTAAATCACCCTAAAGGACTAGCAATTGGTGCAAGACATATTACAGT
GTCTACATCTGGTTTAGTACCTAAGATTAAAGAATTTGCTCATTTAGGTATTCAAGTAAACTTAGCGGTTTCGCTACATG
CACCGAATAATGAAATTAGATCTAAATTAATGAAAATCAATGATAGATTCAAAGTTGAAGAAGTCGTAGATGCAATCAAA
TACTATATTCATGTTACTAACAGAAGAGTTACAATTGAATATATTATGATTCAAGATTTAAATGATAGTGTAGAAACTGC
AGTAGAATTAGCTAAACTCTTAAAGGGTATGAATGTCTATGTGAATTTAATTCCATATAACACCGTTAAAGAAGCAGATT
ATCAAAGATCAAGTCTTGAGAATCGTTTAGCATTCCACAAGACTTTAAAAGAACATAAAATCACTGCAATTTTAAGAAAA
GAACAAGGTCATGACATTAATGCTGCTTGTGGCCAATTAAGAAGTCAAAATCTATAA

Upstream 100 bases:

>100_bases
AATTCAATTTTAAAGCAGTATTTAGAGCATAACTTGATTTCTATTTCACCATATGAAAAATTATCAAAATCTTGTATAAT
AACAAGTGAGGAATAATATT

Downstream 100 bases:

>100_bases
GGAGATACTTACATTTTTGAATAAAGCACGTGTAATTAAATTAATCGGAGGCTTATATAGCTTTAAAGACCTTAAGACGC
AAACAGTGTATGAAGGTTAT

Product: radical SAM superfamily Fe-S oxidoreductase

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MLIYDLTYEELEEFIVENGYKKFRADQIWNWLYKQKIEAFSEMNNIPEDIIKLLNDNYTFAGLETVIKNTSADGTIKFLF
DLKDANLIETVLMSHNYGMSACVTTQVGCNIGCSFCASGVLKKKRDLTAGEIVAQIIRAEKESGVRVSSIVIMGIGEPFD
NYKNFVKFISIVNHPKGLAIGARHITVSTSGLVPKIKEFAHLGIQVNLAVSLHAPNNEIRSKLMKINDRFKVEEVVDAIK
YYIHVTNRRVTIEYIMIQDLNDSVETAVELAKLLKGMNVYVNLIPYNTVKEADYQRSSLENRLAFHKTLKEHKITAILRK
EQGHDINAACGQLRSQNL

Sequences:

>Translated_338_residues
MLIYDLTYEELEEFIVENGYKKFRADQIWNWLYKQKIEAFSEMNNIPEDIIKLLNDNYTFAGLETVIKNTSADGTIKFLF
DLKDANLIETVLMSHNYGMSACVTTQVGCNIGCSFCASGVLKKKRDLTAGEIVAQIIRAEKESGVRVSSIVIMGIGEPFD
NYKNFVKFISIVNHPKGLAIGARHITVSTSGLVPKIKEFAHLGIQVNLAVSLHAPNNEIRSKLMKINDRFKVEEVVDAIK
YYIHVTNRRVTIEYIMIQDLNDSVETAVELAKLLKGMNVYVNLIPYNTVKEADYQRSSLENRLAFHKTLKEHKITAILRK
EQGHDINAACGQLRSQNL
>Mature_338_residues
MLIYDLTYEELEEFIVENGYKKFRADQIWNWLYKQKIEAFSEMNNIPEDIIKLLNDNYTFAGLETVIKNTSADGTIKFLF
DLKDANLIETVLMSHNYGMSACVTTQVGCNIGCSFCASGVLKKKRDLTAGEIVAQIIRAEKESGVRVSSIVIMGIGEPFD
NYKNFVKFISIVNHPKGLAIGARHITVSTSGLVPKIKEFAHLGIQVNLAVSLHAPNNEIRSKLMKINDRFKVEEVVDAIK
YYIHVTNRRVTIEYIMIQDLNDSVETAVELAKLLKGMNVYVNLIPYNTVKEADYQRSSLENRLAFHKTLKEHKITAILRK
EQGHDINAACGQLRSQNL

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=341, Percent_Identity=37.5366568914956, Blast_Score=233, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_ACHLI (A9NEU7)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620253.1
- ProteinModelPortal:   A9NEU7
- GeneID:   5804259
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0251
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- BioCyc:   ALAI441768:ACL_0251-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 38283; Mature: 38283

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIYDLTYEELEEFIVENGYKKFRADQIWNWLYKQKIEAFSEMNNIPEDIIKLLNDNYTF
CEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEE
AGLETVIKNTSADGTIKFLFDLKDANLIETVLMSHNYGMSACVTTQVGCNIGCSFCASGV
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHH
LKKKRDLTAGEIVAQIIRAEKESGVRVSSIVIMGIGEPFDNYKNFVKFISIVNHPKGLAI
HHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEE
GARHITVSTSGLVPKIKEFAHLGIQVNLAVSLHAPNNEIRSKLMKINDRFKVEEVVDAIK
EEEEEEEECCCCCHHHHHHHHCCEEEEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHH
YYIHVTNRRVTIEYIMIQDLNDSVETAVELAKLLKGMNVYVNLIPYNTVKEADYQRSSLE
HHEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHH
NRLAFHKTLKEHKITAILRKEQGHDINAACGQLRSQNL
HHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MLIYDLTYEELEEFIVENGYKKFRADQIWNWLYKQKIEAFSEMNNIPEDIIKLLNDNYTF
CEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEE
AGLETVIKNTSADGTIKFLFDLKDANLIETVLMSHNYGMSACVTTQVGCNIGCSFCASGV
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHH
LKKKRDLTAGEIVAQIIRAEKESGVRVSSIVIMGIGEPFDNYKNFVKFISIVNHPKGLAI
HHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEE
GARHITVSTSGLVPKIKEFAHLGIQVNLAVSLHAPNNEIRSKLMKINDRFKVEEVVDAIK
EEEEEEEECCCCCHHHHHHHHCCEEEEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHH
YYIHVTNRRVTIEYIMIQDLNDSVETAVELAKLLKGMNVYVNLIPYNTVKEADYQRSSLE
HHEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHH
NRLAFHKTLKEHKITAILRKEQGHDINAACGQLRSQNL
HHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA