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The map label for this gene is adhE [H]

Identifier: 162447047

GI number: 162447047

Start: 165156

End: 167744

Strand: Direct

Name: adhE [H]

Synonym: ACL_0177

Alternate gene names: 162447047

Gene position: 165156-167744 (Clockwise)

Preceding gene: 162447046

Following gene: 162447048

Centisome position: 11.03

GC content: 35.15

Gene sequence:

>2589_bases
ATGGAAAAAGACACACTTATCTTAAACGAGGTAGATCTTTTAGTCACGTCTGCTTTAGAAGCACTTGACGAATACATGGA
GTTTACCCAAGAGAAGATTGATGAGATTGTAGCCAAAGTTTCTGTTGAAGCAATTGATGCACATGGCCGTTTAGCCAAGC
TTGCTGTAGAGGAAACTAAACGAGGGGTATTTGAAGATAAAGCAACCAAGAATTTATTTTCAGTAGAATATGTCATCAAT
CATATGAGACACCTAAAAACTGTAGGGATTATCTCGGAAAATACAGTAACGGGTATTACAGAGGTAGCTGATCCTGTTGG
GGTAATTTGCGCTATTACACCAGTAACGAATCCAACATCGACGACAATATTTAAGTCATTGATTGCACTTAAAACTAGAA
ATCCAATCGTCTTTGCGTTTCATCCAAATGCGCAAAATTCATCAAAGGAAGCGGCTAGAATTATTAAAGAAGCAGCGATT
AAATCAGGTGCTCCAAAAAATTGTATTTCATGGATTGAACATCCAAGTTTAGATAATACAAATAAACTTATGAACCACCC
GGGGATTGCAACTATTTTAGCAACTGGTGGTAATGCAATGGTTAAGGCAGCTTACTCATGTGGTAAACCAGCTCTTGGTG
TCGGTGCAGGTAACGTACCAGCATACATTGAAAAAACTGCAAACATTAAAACCGCAATCAATGATATTGTTATGTCTAAG
TCATTTGATAATGGTATGGTATGTGCATCTGAACAAGCAGCTATTATTGATAAAGAAATCTATAAAGAAGCTTTGGCGGA
ATTTAAAAAGTTACATACTTTTTTATGTAACAAAGAACAAAAACAAATGCTGGAAACATTTATGTTCCCAGATGGTGTCA
AGCTTAATCCTTATGTTGTAGGTAAGAGTGCACATCACATTGCAGAACTTGCAGGTTTCGAAGTTCCTTTAAATACCAAC
ATTTTACTAGTAGAATGTAAAAGTGTTGGTAAAGAAGAACCACTTACAAGAGAAAAACTCTCGCCAGTATTAGCAATTCT
TAAATCAAATGATGAAGTACATGGTGTAGCACTTGCAAAAAGTATGGTAGAACTTGATGGTTTAGGACACTCTGCAGTGA
TTCACACACAAAATGATGAACTTAAAAAAACCTTTGGTTTAGCACTTAAAGCGATACGTATTATAGTGAATTCACCAGCG
ACTTTTGGTGGCATAGGTAATGTATATAACAGGTTTATACCATCACTTACCTTAGGGTGTGGTTCTTATGGTAAAAACTC
TGTTTCTGATAATGTCAGTGCCATCCACTTACTTAATATCAAACGTATCGGTGAAAGGAAAAATAATATGCAATGGTTTA
AGGTTCCATCTAAAATTTACTTTGAAAAAGATTCTATTGAATACTTAAGACAAATGAGAGATGTTAACAAGGTTGTCATC
ATCACAGATAGAGTGATGGTTGACTTAGGTTATGTTAAAAAAGTAACTGATCAACTAAGTTTAAGACGTAATCATGTTGT
ATATCAACTATTTACCGATGTTGAAGCTGATCCATCCATTGAAACAGTTCATAAAGGTTATGAACTGATGAAATCCTTCC
AACCAGATACGATTATAGCTATTGGTGGTGGTTCACCAATGGATGCTGCAAAAGCTATGTGGTTATTCTATGAAAACCCT
GAAGTAGATTTTAACGACTTAAAACAAAAATTCATGGATATTAGAAAACGTGCATTTAGATATCCTGATTTAGGTAGAAA
ATCTAAATTAGTATGTATTCCAACAACCTCAGGTACTGGTAGTGAAGTGACTCCGTTTGCAGTTATCACTGATAATAAAG
AGTCTAAAAAATATCCATTAGCAGATTATTCATTAACACCAACTGTAGCTATTATTGACCCTGCACTTACACTTACTCAA
CCAAAATCTGTTACAGCAGATACTGGAATGGACGTGTTAACACATGCAGTAGAAGCTTACGTTTCAGTACTTGCTAATGA
CTATACAGACTCACTTGCAATTCAAGCAGTTAAAATGGTATTTAAATACTTAAAACGTGCATTTGATAATGGATTGGACT
TTGAAGCAAGAGAAAAGATGCATAATGCATCTACCATGGCAGGTATGGCATTTGGTAATGCATTCTTGGGTATATCTCAC
TCTATGGCTCATAAAGTAGGTGGAAGATATCATATACCTCATGGTAGAATTAATGCAATCTTATTACCACATGTCGTTTT
ATATAATGGTACAGTTCCAGCTAAGTTATCTACTTGGCCAAAATACACATATTATGTAGCAGATGAAAAATACATGGAAC
TAGCTAGAGCAATCGGATTAGATCCTAAATCTAAAGAAGAAGGCGTTAAGATGTTTGCTAAGGCATGTCATGATTTAGCA
GTATCATTAGGTATTAATATGAGTTTTGCTAGTCAAAAAGTAGAAGAAAAAGATTTCTTAGAACACAGTGAAACTTTAGC
TTATCTAGCTTATGAGGATCAATGTTCAACAGCTAACCCTAGACTACCTTTAGTAGCGGATATGAAGGTGATCCTAGAAA
ATGCATATTACGGCAAACTAGATATATAG

Upstream 100 bases:

>100_bases
TTTATGAAAACGTATTCATTATAGTTTCATTTGAATTAATTTTAAAATCATATAAAATAGGGCTATAAAACCAATTCATA
TAACACAAGGAGAATTTAAT

Downstream 100 bases:

>100_bases
TATATATTCAAATAATTAAATCAAAGACTTTCAAATAACTTATAGGTTTTAGTAAATCTTGTAAGACAAAAGTTATTTGA
AAGTCTTTTTTTACTATTTA

Product: bifunctional aldehyde/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 862; Mature: 862

Protein sequence:

>862_residues
MEKDTLILNEVDLLVTSALEALDEYMEFTQEKIDEIVAKVSVEAIDAHGRLAKLAVEETKRGVFEDKATKNLFSVEYVIN
HMRHLKTVGIISENTVTGITEVADPVGVICAITPVTNPTSTTIFKSLIALKTRNPIVFAFHPNAQNSSKEAARIIKEAAI
KSGAPKNCISWIEHPSLDNTNKLMNHPGIATILATGGNAMVKAAYSCGKPALGVGAGNVPAYIEKTANIKTAINDIVMSK
SFDNGMVCASEQAAIIDKEIYKEALAEFKKLHTFLCNKEQKQMLETFMFPDGVKLNPYVVGKSAHHIAELAGFEVPLNTN
ILLVECKSVGKEEPLTREKLSPVLAILKSNDEVHGVALAKSMVELDGLGHSAVIHTQNDELKKTFGLALKAIRIIVNSPA
TFGGIGNVYNRFIPSLTLGCGSYGKNSVSDNVSAIHLLNIKRIGERKNNMQWFKVPSKIYFEKDSIEYLRQMRDVNKVVI
ITDRVMVDLGYVKKVTDQLSLRRNHVVYQLFTDVEADPSIETVHKGYELMKSFQPDTIIAIGGGSPMDAAKAMWLFYENP
EVDFNDLKQKFMDIRKRAFRYPDLGRKSKLVCIPTTSGTGSEVTPFAVITDNKESKKYPLADYSLTPTVAIIDPALTLTQ
PKSVTADTGMDVLTHAVEAYVSVLANDYTDSLAIQAVKMVFKYLKRAFDNGLDFEAREKMHNASTMAGMAFGNAFLGISH
SMAHKVGGRYHIPHGRINAILLPHVVLYNGTVPAKLSTWPKYTYYVADEKYMELARAIGLDPKSKEEGVKMFAKACHDLA
VSLGINMSFASQKVEEKDFLEHSETLAYLAYEDQCSTANPRLPLVADMKVILENAYYGKLDI

Sequences:

>Translated_862_residues
MEKDTLILNEVDLLVTSALEALDEYMEFTQEKIDEIVAKVSVEAIDAHGRLAKLAVEETKRGVFEDKATKNLFSVEYVIN
HMRHLKTVGIISENTVTGITEVADPVGVICAITPVTNPTSTTIFKSLIALKTRNPIVFAFHPNAQNSSKEAARIIKEAAI
KSGAPKNCISWIEHPSLDNTNKLMNHPGIATILATGGNAMVKAAYSCGKPALGVGAGNVPAYIEKTANIKTAINDIVMSK
SFDNGMVCASEQAAIIDKEIYKEALAEFKKLHTFLCNKEQKQMLETFMFPDGVKLNPYVVGKSAHHIAELAGFEVPLNTN
ILLVECKSVGKEEPLTREKLSPVLAILKSNDEVHGVALAKSMVELDGLGHSAVIHTQNDELKKTFGLALKAIRIIVNSPA
TFGGIGNVYNRFIPSLTLGCGSYGKNSVSDNVSAIHLLNIKRIGERKNNMQWFKVPSKIYFEKDSIEYLRQMRDVNKVVI
ITDRVMVDLGYVKKVTDQLSLRRNHVVYQLFTDVEADPSIETVHKGYELMKSFQPDTIIAIGGGSPMDAAKAMWLFYENP
EVDFNDLKQKFMDIRKRAFRYPDLGRKSKLVCIPTTSGTGSEVTPFAVITDNKESKKYPLADYSLTPTVAIIDPALTLTQ
PKSVTADTGMDVLTHAVEAYVSVLANDYTDSLAIQAVKMVFKYLKRAFDNGLDFEAREKMHNASTMAGMAFGNAFLGISH
SMAHKVGGRYHIPHGRINAILLPHVVLYNGTVPAKLSTWPKYTYYVADEKYMELARAIGLDPKSKEEGVKMFAKACHDLA
VSLGINMSFASQKVEEKDFLEHSETLAYLAYEDQCSTANPRLPLVADMKVILENAYYGKLDI
>Mature_862_residues
MEKDTLILNEVDLLVTSALEALDEYMEFTQEKIDEIVAKVSVEAIDAHGRLAKLAVEETKRGVFEDKATKNLFSVEYVIN
HMRHLKTVGIISENTVTGITEVADPVGVICAITPVTNPTSTTIFKSLIALKTRNPIVFAFHPNAQNSSKEAARIIKEAAI
KSGAPKNCISWIEHPSLDNTNKLMNHPGIATILATGGNAMVKAAYSCGKPALGVGAGNVPAYIEKTANIKTAINDIVMSK
SFDNGMVCASEQAAIIDKEIYKEALAEFKKLHTFLCNKEQKQMLETFMFPDGVKLNPYVVGKSAHHIAELAGFEVPLNTN
ILLVECKSVGKEEPLTREKLSPVLAILKSNDEVHGVALAKSMVELDGLGHSAVIHTQNDELKKTFGLALKAIRIIVNSPA
TFGGIGNVYNRFIPSLTLGCGSYGKNSVSDNVSAIHLLNIKRIGERKNNMQWFKVPSKIYFEKDSIEYLRQMRDVNKVVI
ITDRVMVDLGYVKKVTDQLSLRRNHVVYQLFTDVEADPSIETVHKGYELMKSFQPDTIIAIGGGSPMDAAKAMWLFYENP
EVDFNDLKQKFMDIRKRAFRYPDLGRKSKLVCIPTTSGTGSEVTPFAVITDNKESKKYPLADYSLTPTVAIIDPALTLTQ
PKSVTADTGMDVLTHAVEAYVSVLANDYTDSLAIQAVKMVFKYLKRAFDNGLDFEAREKMHNASTMAGMAFGNAFLGISH
SMAHKVGGRYHIPHGRINAILLPHVVLYNGTVPAKLSTWPKYTYYVADEKYMELARAIGLDPKSKEEGVKMFAKACHDLA
VSLGINMSFASQKVEEKDFLEHSETLAYLAYEDQCSTANPRLPLVADMKVILENAYYGKLDI

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=329, Percent_Identity=31.306990881459, Blast_Score=129, Evalue=1e-29,
Organism=Escherichia coli, GI1787493, Length=861, Percent_Identity=46.2253193960511, Blast_Score=777, Evalue=0.0,
Organism=Escherichia coli, GI48994951, Length=397, Percent_Identity=35.264483627204, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI87082107, Length=411, Percent_Identity=33.8199513381995, Blast_Score=205, Evalue=8e-54,
Organism=Escherichia coli, GI1789163, Length=402, Percent_Identity=32.5870646766169, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1788797, Length=379, Percent_Identity=31.1345646437995, Blast_Score=163, Evalue=5e-41,
Organism=Escherichia coli, GI1789386, Length=315, Percent_Identity=26.031746031746, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17537053, Length=279, Percent_Identity=28.673835125448, Blast_Score=130, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321181, Length=418, Percent_Identity=32.0574162679426, Blast_Score=197, Evalue=6e-51,
Organism=Drosophila melanogaster, GI24657991, Length=334, Percent_Identity=32.0359281437126, Blast_Score=124, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 95110; Mature: 95110

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDTLILNEVDLLVTSALEALDEYMEFTQEKIDEIVAKVSVEAIDAHGRLAKLAVEETK
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RGVFEDKATKNLFSVEYVINHMRHLKTVGIISENTVTGITEVADPVGVICAITPVTNPTS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHCCCCEEEEEEECCCCCCH
TTIFKSLIALKTRNPIVFAFHPNAQNSSKEAARIIKEAAIKSGAPKNCISWIEHPSLDNT
HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
NKLMNHPGIATILATGGNAMVKAAYSCGKPALGVGAGNVPAYIEKTANIKTAINDIVMSK
HHHHCCCCEEEEEECCCCCEEHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHC
SFDNGMVCASEQAAIIDKEIYKEALAEFKKLHTFLCNKEQKQMLETFMFPDGVKLNPYVV
CCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCEECCEEE
GKSAHHIAELAGFEVPLNTNILLVECKSVGKEEPLTREKLSPVLAILKSNDEVHGVALAK
CCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
SMVELDGLGHSAVIHTQNDELKKTFGLALKAIRIIVNSPATFGGIGNVYNRFIPSLTLGC
HHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEECC
GSYGKNSVSDNVSAIHLLNIKRIGERKNNMQWFKVPSKIYFEKDSIEYLRQMRDVNKVVI
CCCCCCCCCCCCCEEEEEEHHHHCCCCCCCEEEECCCCEEECCHHHHHHHHHHCCCEEEE
ITDRVMVDLGYVKKVTDQLSLRRNHVVYQLFTDVEADPSIETVHKGYELMKSFQPDTIIA
EECHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEE
IGGGSPMDAAKAMWLFYENPEVDFNDLKQKFMDIRKRAFRYPDLGRKSKLVCIPTTSGTG
ECCCCCCCHHCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
SEVTPFAVITDNKESKKYPLADYSLTPTVAIIDPALTLTQPKSVTADTGMDVLTHAVEAY
CCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHH
VSVLANDYTDSLAIQAVKMVFKYLKRAFDNGLDFEAREKMHNASTMAGMAFGNAFLGISH
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCH
SMAHKVGGRYHIPHGRINAILLPHVVLYNGTVPAKLSTWPKYTYYVADEKYMELARAIGL
HHHHHCCCEEECCCCCCCEEEECEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHCC
DPKSKEEGVKMFAKACHDLAVSLGINMSFASQKVEEKDFLEHSETLAYLAYEDQCSTANP
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
RLPLVADMKVILENAYYGKLDI
CCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEKDTLILNEVDLLVTSALEALDEYMEFTQEKIDEIVAKVSVEAIDAHGRLAKLAVEETK
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RGVFEDKATKNLFSVEYVINHMRHLKTVGIISENTVTGITEVADPVGVICAITPVTNPTS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHCCCCEEEEEEECCCCCCH
TTIFKSLIALKTRNPIVFAFHPNAQNSSKEAARIIKEAAIKSGAPKNCISWIEHPSLDNT
HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
NKLMNHPGIATILATGGNAMVKAAYSCGKPALGVGAGNVPAYIEKTANIKTAINDIVMSK
HHHHCCCCEEEEEECCCCCEEHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHC
SFDNGMVCASEQAAIIDKEIYKEALAEFKKLHTFLCNKEQKQMLETFMFPDGVKLNPYVV
CCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCEECCEEE
GKSAHHIAELAGFEVPLNTNILLVECKSVGKEEPLTREKLSPVLAILKSNDEVHGVALAK
CCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
SMVELDGLGHSAVIHTQNDELKKTFGLALKAIRIIVNSPATFGGIGNVYNRFIPSLTLGC
HHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEECC
GSYGKNSVSDNVSAIHLLNIKRIGERKNNMQWFKVPSKIYFEKDSIEYLRQMRDVNKVVI
CCCCCCCCCCCCCEEEEEEHHHHCCCCCCCEEEECCCCEEECCHHHHHHHHHHCCCEEEE
ITDRVMVDLGYVKKVTDQLSLRRNHVVYQLFTDVEADPSIETVHKGYELMKSFQPDTIIA
EECHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEE
IGGGSPMDAAKAMWLFYENPEVDFNDLKQKFMDIRKRAFRYPDLGRKSKLVCIPTTSGTG
ECCCCCCCHHCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
SEVTPFAVITDNKESKKYPLADYSLTPTVAIIDPALTLTQPKSVTADTGMDVLTHAVEAY
CCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHH
VSVLANDYTDSLAIQAVKMVFKYLKRAFDNGLDFEAREKMHNASTMAGMAFGNAFLGISH
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCH
SMAHKVGGRYHIPHGRINAILLPHVVLYNGTVPAKLSTWPKYTYYVADEKYMELARAIGL
HHHHHCCCEEECCCCCCCEEEECEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHCC
DPKSKEEGVKMFAKACHDLAVSLGINMSFASQKVEEKDFLEHSETLAYLAYEDQCSTANP
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
RLPLVADMKVILENAYYGKLDI
CCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]