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The map label for this gene is tmk

Identifier: 162446997

GI number: 162446997

Start: 108887

End: 109492

Strand: Direct

Name: tmk

Synonym: ACL_0127

Alternate gene names: 162446997

Gene position: 108887-109492 (Clockwise)

Preceding gene: 162446996

Following gene: 162446998

Centisome position: 7.27

GC content: 32.84

Gene sequence:

>606_bases
ATGTTTATTACATTTGAAGGCGGAGAAGGTAGCGGTAAAACGACCTTAATCGAAAAACTAAAACACACATTACTAGAGAA
AAAATACGATGTACTAACCACGCGTGAACCTGGTGGATCTAAGGTTGCTGAAAAAATTCGTTCTGTATTACTGGATAATA
AAAACACAGAAATCACTGCACATACAGAAGCTCTTTTATTTGCAGCAAGTAGAGCACAACATTTAGATGAAGTCATCATT
CCTAATTTAGATAAAGTCATACTTTGTGACCGATATATCGATTCATCCTATGCGTATCAAGCGTTTGGTAGAAACCTAGG
TATGGAGTTTGTTCAAAGTATTAATAGTTATGCATTAAAATACTTACCTGACTTAACATTTTATATCGACTTAGATCCTA
AAACAGGTATTGATAGAGTAAAGAAAAACAGATTACATAAAACAGATAGATTAGACATGGAAGTTCAAACATTTCATCAA
AAGGTACGTGAAGGTTATATTCGCATTTCAGAAATGTTTAAAGAACGTATCGTCGTGATTGATGGTAATCAATCGATTGA
TGCAATCTATCAAATTATAATGGATAACATATTAAAACGCCTATGA

Upstream 100 bases:

>100_bases
TTGAAACTAAAAATAGAGAACTATCTGCAAAAACAGCAGATGCTAAAGGACTATATTTAAAACATATTTATTATTAATAT
TAGAAAAAGGGGCATGCATT

Downstream 100 bases:

>100_bases
ATAAAATAGTTGAAAGATTTAAATATGCAATTGAAAAAGAAAGACTTTCCCACTTATATGTTATAAGTGGGTCTTTAGGT
CTTACCAAGCAAAATCTAGT

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MFITFEGGEGSGKTTLIEKLKHTLLEKKYDVLTTREPGGSKVAEKIRSVLLDNKNTEITAHTEALLFAASRAQHLDEVII
PNLDKVILCDRYIDSSYAYQAFGRNLGMEFVQSINSYALKYLPDLTFYIDLDPKTGIDRVKKNRLHKTDRLDMEVQTFHQ
KVREGYIRISEMFKERIVVIDGNQSIDAIYQIIMDNILKRL

Sequences:

>Translated_201_residues
MFITFEGGEGSGKTTLIEKLKHTLLEKKYDVLTTREPGGSKVAEKIRSVLLDNKNTEITAHTEALLFAASRAQHLDEVII
PNLDKVILCDRYIDSSYAYQAFGRNLGMEFVQSINSYALKYLPDLTFYIDLDPKTGIDRVKKNRLHKTDRLDMEVQTFHQ
KVREGYIRISEMFKERIVVIDGNQSIDAIYQIIMDNILKRL
>Mature_201_residues
MFITFEGGEGSGKTTLIEKLKHTLLEKKYDVLTTREPGGSKVAEKIRSVLLDNKNTEITAHTEALLFAASRAQHLDEVII
PNLDKVILCDRYIDSSYAYQAFGRNLGMEFVQSINSYALKYLPDLTFYIDLDPKTGIDRVKKNRLHKTDRLDMEVQTFHQ
KVREGYIRISEMFKERIVVIDGNQSIDAIYQIIMDNILKRL

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:
GO:0000166: Thymidylate kinase
GO:0004798: Thymidylate kinase
GO:0005524: Thymidylate kinase
GO:0006233: Thymidylate kinase
GO:0009165: Thymidylate kinase
GO:0016301: Thymidylate kinase
GO:0016310: Thymidylate kinase
GO:0016740: Thymidylate kinase

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=203, Percent_Identity=33.9901477832512, Blast_Score=104, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_ACHLI (A9NEH3)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620129.1
- ProteinModelPortal:   A9NEH3
- SMR:   A9NEH3
- GeneID:   5803553
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0127
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- BioCyc:   ALAI441768:ACL_0127-MONOMER
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 23148; Mature: 23148

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFITFEGGEGSGKTTLIEKLKHTLLEKKYDVLTTREPGGSKVAEKIRSVLLDNKNTEITA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEE
HTEALLFAASRAQHLDEVIIPNLDKVILCDRYIDSSYAYQAFGRNLGMEFVQSINSYALK
HHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHH
YLPDLTFYIDLDPKTGIDRVKKNRLHKTDRLDMEVQTFHQKVREGYIRISEMFKERIVVI
HCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEE
DGNQSIDAIYQIIMDNILKRL
ECCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MFITFEGGEGSGKTTLIEKLKHTLLEKKYDVLTTREPGGSKVAEKIRSVLLDNKNTEITA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEE
HTEALLFAASRAQHLDEVIIPNLDKVILCDRYIDSSYAYQAFGRNLGMEFVQSINSYALK
HHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHH
YLPDLTFYIDLDPKTGIDRVKKNRLHKTDRLDMEVQTFHQKVREGYIRISEMFKERIVVI
HCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEE
DGNQSIDAIYQIIMDNILKRL
ECCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA