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The map label for this gene is truA [H]

Identifier: 162446996

GI number: 162446996

Start: 108141

End: 108863

Strand: Direct

Name: truA [H]

Synonym: ACL_0126

Alternate gene names: 162446996

Gene position: 108141-108863 (Clockwise)

Preceding gene: 162446995

Following gene: 162446997

Centisome position: 7.22

GC content: 31.4

Gene sequence:

>723_bases
TTGCGTTATAAGGCAATTTTCAGTTATGATGGCACAAATTATAGTGGCTATCAAAAACAAATTAATCAATTAGGTATTCA
AACAGTTATCGAAAAAGCATTTAGGCTGATGACTCAAATAGATATAGAAACCTTTGCCGCCTCTAGAACAGATAAGGGGG
TACATGCATTACATCAAGTACTTCATTTTGATTCAGATATTGAGTTAACATCGGATCAATGGGTAGATGCTTTAAATAAA
AGACTACCTACAGATATTAGACTGATGAAAGTTACACCAGTAAAGGATAACTTTCATGCAAGACACAGTGCAAAGTCTAA
AAGATATATTTATAAAATAGCCAAACAACCATCAACAGTCTTTAGAGGTAACCACGAACTTTATGTGAAGCACTTTGACA
TTGATCTTGTTAAAGATCATTTGCATACAATTGTAGGTACACACGACTTTAGCGGGTTTAGTCCAAACAAAGAACATAAA
CCACCAATCAAAACTATTTATAGTTTTGATTATAAAGAAACCAAAACTCATTATATATTTAGCATTCATGGCAACAGCTT
TTTAAGATATATGGTGCGTATTATTATGGGGAATGTGATTGCTGTAGCAACAAATAAAAAACCTCAAAACCAACTAGAAA
TATTACTTGAAACTAAAAATAGAGAACTATCTGCAAAAACAGCAGATGCTAAAGGACTATATTTAAAACATATTTATTAT
TAA

Upstream 100 bases:

>100_bases
GAACCTAGAACGAAATTGGATGTCTATAAAATCAACTATAAAGATATTATAATTTTAAGTGTTGTCATACTCATAATTAT
TGGATTGTTGGTGACTAGAT

Downstream 100 bases:

>100_bases
TATTAGAAAAAGGGGCATGCATTATGTTTATTACATTTGAAGGCGGAGAAGGTAGCGGTAAAACGACCTTAATCGAAAAA
CTAAAACACACATTACTAGA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRYKAIFSYDGTNYSGYQKQINQLGIQTVIEKAFRLMTQIDIETFAASRTDKGVHALHQVLHFDSDIELTSDQWVDALNK
RLPTDIRLMKVTPVKDNFHARHSAKSKRYIYKIAKQPSTVFRGNHELYVKHFDIDLVKDHLHTIVGTHDFSGFSPNKEHK
PPIKTIYSFDYKETKTHYIFSIHGNSFLRYMVRIIMGNVIAVATNKKPQNQLEILLETKNRELSAKTADAKGLYLKHIYY

Sequences:

>Translated_240_residues
MRYKAIFSYDGTNYSGYQKQINQLGIQTVIEKAFRLMTQIDIETFAASRTDKGVHALHQVLHFDSDIELTSDQWVDALNK
RLPTDIRLMKVTPVKDNFHARHSAKSKRYIYKIAKQPSTVFRGNHELYVKHFDIDLVKDHLHTIVGTHDFSGFSPNKEHK
PPIKTIYSFDYKETKTHYIFSIHGNSFLRYMVRIIMGNVIAVATNKKPQNQLEILLETKNRELSAKTADAKGLYLKHIYY
>Mature_240_residues
MRYKAIFSYDGTNYSGYQKQINQLGIQTVIEKAFRLMTQIDIETFAASRTDKGVHALHQVLHFDSDIELTSDQWVDALNK
RLPTDIRLMKVTPVKDNFHARHSAKSKRYIYKIAKQPSTVFRGNHELYVKHFDIDLVKDHLHTIVGTHDFSGFSPNKEHK
PPIKTIYSFDYKETKTHYIFSIHGNSFLRYMVRIIMGNVIAVATNKKPQNQLEILLETKNRELSAKTADAKGLYLKHIYY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=273, Percent_Identity=26.7399267399267, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1788657, Length=242, Percent_Identity=32.6446280991736, Blast_Score=137, Evalue=8e-34,
Organism=Drosophila melanogaster, GI116007752, Length=303, Percent_Identity=27.7227722772277, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281362146, Length=266, Percent_Identity=29.6992481203008, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648455, Length=266, Percent_Identity=29.6992481203008, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27935; Mature: 27935

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYKAIFSYDGTNYSGYQKQINQLGIQTVIEKAFRLMTQIDIETFAASRTDKGVHALHQV
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LHFDSDIELTSDQWVDALNKRLPTDIRLMKVTPVKDNFHARHSAKSKRYIYKIAKQPSTV
HHCCCCCEECHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCHHHHHHHHHCCCCCE
FRGNHELYVKHFDIDLVKDHLHTIVGTHDFSGFSPNKEHKPPIKTIYSFDYKETKTHYIF
ECCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEECCCCCCCEEEEE
SIHGNSFLRYMVRIIMGNVIAVATNKKPQNQLEILLETKNRELSAKTADAKGLYLKHIYY
EEECCHHHHHHHHHHHCCEEEEEECCCCHHHEEEEEECCCCEEECCCCCCCCEEEEEECC
>Mature Secondary Structure
MRYKAIFSYDGTNYSGYQKQINQLGIQTVIEKAFRLMTQIDIETFAASRTDKGVHALHQV
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LHFDSDIELTSDQWVDALNKRLPTDIRLMKVTPVKDNFHARHSAKSKRYIYKIAKQPSTV
HHCCCCCEECHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCHHHHHHHHHCCCCCE
FRGNHELYVKHFDIDLVKDHLHTIVGTHDFSGFSPNKEHKPPIKTIYSFDYKETKTHYIF
ECCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEECCCCCCCEEEEE
SIHGNSFLRYMVRIIMGNVIAVATNKKPQNQLEILLETKNRELSAKTADAKGLYLKHIYY
EEECCHHHHHHHHHHHCCEEEEEECCCCHHHEEEEEECCCCEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA