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The map label for this gene is pyrB [H]

Identifier: 162210118

GI number: 162210118

Start: 298871

End: 300166

Strand: Direct

Name: pyrB [H]

Synonym: BURPS1710b_A0197

Alternate gene names: 162210118

Gene position: 298871-300166 (Clockwise)

Preceding gene: 76818914

Following gene: 76817444

Centisome position: 9.39

GC content: 66.59

Gene sequence:

>1296_bases
ATGACCGTTCCTCAGCAAGCGTTCCTGCGCGACGCGATGCGCCGCCTGAACATGACCCGCGAAGCGTTCGCGAACCGCAT
CGGCGTGAGCCGGCGCGCCCTCGACACATGGCTGTTGCCGGACGATTCGCAGGAATCGCGCGGCATGCCCGAGATCGTCG
AGCGTTTCGTCTCCGAAATCGTCGAGCGCGCGCCGGCCGCCGACGGATATGCGCAAAGCGTAGATTCTCGACAGCCGCTT
GCGCAGCAGTTCCTGTTCGAAGGCCGGCCGCAGTTGCTGTCGGTCGACCAGTTCTCGCGCGATTCCGTCGAAGCGCTGTT
CCGGGTCGCCGACGTGATGCAGCCGATCGCGCGCCGCCGCAAGATCTCGCGCGTGCTCGAGGGCGCGGTGCTCGGCAACC
TGTTCTTCGAGGCGAGCACGCGCACGCGCGTGTCGTTCGGCGCCGCGTTCTGCAGGCTCGGCGGCTCGGTGTGCGACACG
ACGGGCTTCACCTTCTCGTCGATGGCCAAGGGTGAATCGATCTACGATACGAGCCGCGTGATGGCCGGCTACGTCGACGC
GCTCGTGATCCGCCATCCGGAACAAGGCTCGGTCGCCGAATTCGCGCGCGCGACCAACCTGCCCGTGATCAACGGCGGCG
ACGGCCCCGGCGAGCATCCGAGCCAGGCGCTCCTCGATCTGTATACGATCCAGCGCGAATTCTCGCGCCTGGGCAAGATC
GTCGACGGCGCGTGCATCGCGCTCGTCGGCGACCTGAAGTACGGCCGCACGGTCCACTCGCTCGTCAAGCTGCTCGCGCT
GTATCGCGGGCTCAAGTTCACGCTCGTATCGCCGCCGACGCTCGAGATGCCGGCCTACATCGTCGATCAGATCTCGAAGA
ACGGTCACGTCGTCGAGGAAACGCACGACCTCGCGGCGGGCCTGAAGGGCGCGGACGTCGTCTACGCGACGCGAATCCAG
AAGGAGCGCTTCACCGACGAATCGTTCGAAGGCTATACGCCCGACTTCCAGATCAATCAGGCACGGGTCGACGCGGCCTG
CGGCCCCGACACGCTGATCATGCACCCGCTGCCGCGCGACAGCCGGCCCGGTGCGAACGATCTGTCGACCGATCTGAACC
GCGACCCGCGCCTCGCGATCTTCCGCCAGACCGACAACGGGATTCCGGTGCGCATGGCGATCTTCGCGGTGCTGCTCGGC
GTCGAGCATCTCGTCCAACACTCGATGCGCGACGCGACGTGGCGTCCACCCACGTACCTCGGGCCGGAGGACGCGGTGTT
CCACGGGATCGACTAA

Upstream 100 bases:

>100_bases
CGATTCCGCCTTCGTCAATCCGCGCGCACTTTCCGCTTGACTTCGGCTGCCGACGACCTACCATACGCATCGCGTATATT
TCAGAGAGCCCAGCCGACCG

Downstream 100 bases:

>100_bases
CGGTTCCACGCGCGGGCATGCCCGCGCCCGCCGCGTGCGGCGGCCGCCGCGGCCGGGCCCGCCCCGCTCGCATGCGTCGC
TCGCGCGCCTGACGCATGCG

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTVPQQAFLRDAMRRLNMTREAFANRIGVSRRALDTWLLPDDSQESRGMPEIVERFVSEIVERAPAADGYAQSVDSRQPL
AQQFLFEGRPQLLSVDQFSRDSVEALFRVADVMQPIARRRKISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDT
TGFTFSSMAKGESIYDTSRVMAGYVDALVIRHPEQGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKI
VDGACIALVGDLKYGRTVHSLVKLLALYRGLKFTLVSPPTLEMPAYIVDQISKNGHVVEETHDLAAGLKGADVVYATRIQ
KERFTDESFEGYTPDFQINQARVDAACGPDTLIMHPLPRDSRPGANDLSTDLNRDPRLAIFRQTDNGIPVRMAIFAVLLG
VEHLVQHSMRDATWRPPTYLGPEDAVFHGID

Sequences:

>Translated_431_residues
MTVPQQAFLRDAMRRLNMTREAFANRIGVSRRALDTWLLPDDSQESRGMPEIVERFVSEIVERAPAADGYAQSVDSRQPL
AQQFLFEGRPQLLSVDQFSRDSVEALFRVADVMQPIARRRKISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDT
TGFTFSSMAKGESIYDTSRVMAGYVDALVIRHPEQGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKI
VDGACIALVGDLKYGRTVHSLVKLLALYRGLKFTLVSPPTLEMPAYIVDQISKNGHVVEETHDLAAGLKGADVVYATRIQ
KERFTDESFEGYTPDFQINQARVDAACGPDTLIMHPLPRDSRPGANDLSTDLNRDPRLAIFRQTDNGIPVRMAIFAVLLG
VEHLVQHSMRDATWRPPTYLGPEDAVFHGID
>Mature_430_residues
TVPQQAFLRDAMRRLNMTREAFANRIGVSRRALDTWLLPDDSQESRGMPEIVERFVSEIVERAPAADGYAQSVDSRQPLA
QQFLFEGRPQLLSVDQFSRDSVEALFRVADVMQPIARRRKISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDTT
GFTFSSMAKGESIYDTSRVMAGYVDALVIRHPEQGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKIV
DGACIALVGDLKYGRTVHSLVKLLALYRGLKFTLVSPPTLEMPAYIVDQISKNGHVVEETHDLAAGLKGADVVYATRIQK
ERFTDESFEGYTPDFQINQARVDAACGPDTLIMHPLPRDSRPGANDLSTDLNRDPRLAIFRQTDNGIPVRMAIFAVLLGV
EHLVQHSMRDATWRPPTYLGPEDAVFHGID

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=41.8006430868167, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI38788445, Length=335, Percent_Identity=28.3582089552239, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI2367364, Length=316, Percent_Identity=36.0759493670886, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1790703, Length=279, Percent_Identity=27.2401433691756, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786469, Length=281, Percent_Identity=28.1138790035587, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=331, Percent_Identity=42.5981873111783, Blast_Score=230, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=36.9897959183673, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6322373, Length=334, Percent_Identity=26.0479041916168, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24642586, Length=333, Percent_Identity=38.7387387387387, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 47715; Mature: 47584

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPQQAFLRDAMRRLNMTREAFANRIGVSRRALDTWLLPDDSQESRGMPEIVERFVSEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHH
VERAPAADGYAQSVDSRQPLAQQFLFEGRPQLLSVDQFSRDSVEALFRVADVMQPIARRR
HHHCCCCCCHHHHCCCCCHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHH
KISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDTTGFTFSSMAKGESIYDTSRV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHCCCCCHHHHHCCCHHHHHHHH
MAGYVDALVIRHPEQGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKI
HHHHHHHHHEECCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VDGACIALVGDLKYGRTVHSLVKLLALYRGLKFTLVSPPTLEMPAYIVDQISKNGHVVEE
HCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCCCEEHH
THDLAAGLKGADVVYATRIQKERFTDESFEGYTPDFQINQARVDAACGPDTLIMHPLPRD
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECHHHCCCCCCCCCEEECCCCCC
SRPGANDLSTDLNRDPRLAIFRQTDNGIPVRMAIFAVLLGVEHLVQHSMRDATWRPPTYL
CCCCCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPEDAVFHGID
CCCCCCCCCCC
>Mature Secondary Structure 
TVPQQAFLRDAMRRLNMTREAFANRIGVSRRALDTWLLPDDSQESRGMPEIVERFVSEI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHH
VERAPAADGYAQSVDSRQPLAQQFLFEGRPQLLSVDQFSRDSVEALFRVADVMQPIARRR
HHHCCCCCCHHHHCCCCCHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHH
KISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDTTGFTFSSMAKGESIYDTSRV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHCCCCCHHHHHCCCHHHHHHHH
MAGYVDALVIRHPEQGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKI
HHHHHHHHHEECCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VDGACIALVGDLKYGRTVHSLVKLLALYRGLKFTLVSPPTLEMPAYIVDQISKNGHVVEE
HCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCCCEEHH
THDLAAGLKGADVVYATRIQKERFTDESFEGYTPDFQINQARVDAACGPDTLIMHPLPRD
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECHHHCCCCCCCCCEEECCCCCC
SRPGANDLSTDLNRDPRLAIFRQTDNGIPVRMAIFAVLLGVEHLVQHSMRDATWRPPTYL
CCCCCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPEDAVFHGID
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA