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The map label for this gene is hsrA [H]

Identifier: 162149052

GI number: 162149052

Start: 3374079

End: 3375461

Strand: Direct

Name: hsrA [H]

Synonym: GDI_3282

Alternate gene names: 162149052

Gene position: 3374079-3375461 (Clockwise)

Preceding gene: 162149051

Following gene: 162149064

Centisome position: 85.55

GC content: 69.2

Gene sequence:

>1383_bases
ATGACCCTGCTGCCCTATATCGTCGCCGCCACGTTCTTCATGGAGTATCTGGACTCCACGATCATCGCGACGGCGCTACC
GTCCATGGCGCGGTCCTTCCATGTGGGGCCGAACGAAATCAGCTTGGGCATGACCGCCTATATGCTGACGCTGGCGGTCT
TCATCCCGATCAGCGGCTGGGCGGCCGACCGGTTCGGGTCGCGCACGGTCTTCGCCAGCGCGGTCGTGGTCTTCACCGCC
GCCTCGGTCCTGTGCGGGTTCGCGGGCAGCGTGGACCAGTTCGTCGCCGCCCGCGTGCTGCAGGGCCTGGGCGGGGCGAT
GATGGTGCCGGTCGGCCGGTTCATCGTGGTGCGCAACACCGAAACCAGCCGGATGATCCAGGCGATTTCCACCATCACCT
GGCCGGCGGTGGTGGCCCCGGTGGTGGGGCCGACGGTCGGCGGCATGATCGTGACGTTCGCCACCTGGCACTGGATCTTC
TTCATCAACGTGCCGCTGGGGCTGATGGTGCTGGGCGTCATCTTCGCCGTGGTGCCCCAGCAGTTCGGCGAGCGCCGGCC
GATGGACTGGGGCGGCTTCGTGCTGGGCGGCCTGTCGCTGACCGCGATCCTGCTGGGCACGGAACTGGCCAGCAACACCC
AGGCCAGCCTGGTCCTGGCGGGGGCGCTGTTCGTGGCCGGCCTGCTGCTGGGCGCGGCCACGCTGCGCCACGCGGCGGGG
CAGGACCATCCGCTGCTGGATTTCCGGCTGATGCGGCATCCGACCTTCGCCGTCACCGTGCTGGCCGGCACGCTCAGCCG
CTTCACCATCGACGCCATCCCCTATCTGGTGCCGCTGCTGTTGCAGGTAGGGTTCGGGCTGACGGCCTTTCACGCCGGGT
CGCTGATGCTGGCCTTCGCCTTCGGCAACCTGGGCATGAAGATGTTCACGACGCGCATCCTGGACCGCTTCGGCTTCCGC
ACCACGGCGGCGGTGAACGCGGGGCTGGGCGTGCTGTTCGTCCTGCTGGCGGGGGCGATGGTCCCGGCGACGCCGGTCGC
GATCCTGCTGCTGGTGCTGCTGGGCTGCGGGGTCACACGGTCCATGCAGTACACACTGCTGGCCACGCTGGGCTACGCCG
ATATCGGCAATCGTGAAAAAGGCGCGGCCAGCACGCTGCTCAGCGTCACCCGGCAGATCTGCATCGGGCTGGGCGTGGCC
TTCGCGGCGCTGACGCTGCGCGCCGTCGCCACCCTGCGGGGCGAACACCCGGCCACCGACGCCTTCGTCCCGGCCGATTT
CCACTGGGCGTTCCTGATCGTCGCCATCCCGCTGGCCCTGTCGATCCCGCGCTACCTGCGCATGGACCGCACGGCCGGGG
CGGCGCTACGCACCGCTTCCTGA

Upstream 100 bases:

>100_bases
GTCGGGGTGCGGATGCGCCACCTGCCCATCCGTCCGGCGGATGTGCTGCATGCCCTGCGGAGCAGGAACGGCACCGCATC
AACCTGAAACCGGCCGCGCC

Downstream 100 bases:

>100_bases
TATCTGGGACGCCGCCTGCCGGATGGCGCGCGCCATCGCCTCCACCCCGTTTCCCCGGTTGGTGGACAGCGCCTGCACCA
GTCCGAGATCGCGCAGGAAG

Product: transporter protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTLLPYIVAATFFMEYLDSTIIATALPSMARSFHVGPNEISLGMTAYMLTLAVFIPISGWAADRFGSRTVFASAVVVFTA
ASVLCGFAGSVDQFVAARVLQGLGGAMMVPVGRFIVVRNTETSRMIQAISTITWPAVVAPVVGPTVGGMIVTFATWHWIF
FINVPLGLMVLGVIFAVVPQQFGERRPMDWGGFVLGGLSLTAILLGTELASNTQASLVLAGALFVAGLLLGAATLRHAAG
QDHPLLDFRLMRHPTFAVTVLAGTLSRFTIDAIPYLVPLLLQVGFGLTAFHAGSLMLAFAFGNLGMKMFTTRILDRFGFR
TTAAVNAGLGVLFVLLAGAMVPATPVAILLLVLLGCGVTRSMQYTLLATLGYADIGNREKGAASTLLSVTRQICIGLGVA
FAALTLRAVATLRGEHPATDAFVPADFHWAFLIVAIPLALSIPRYLRMDRTAGAALRTAS

Sequences:

>Translated_460_residues
MTLLPYIVAATFFMEYLDSTIIATALPSMARSFHVGPNEISLGMTAYMLTLAVFIPISGWAADRFGSRTVFASAVVVFTA
ASVLCGFAGSVDQFVAARVLQGLGGAMMVPVGRFIVVRNTETSRMIQAISTITWPAVVAPVVGPTVGGMIVTFATWHWIF
FINVPLGLMVLGVIFAVVPQQFGERRPMDWGGFVLGGLSLTAILLGTELASNTQASLVLAGALFVAGLLLGAATLRHAAG
QDHPLLDFRLMRHPTFAVTVLAGTLSRFTIDAIPYLVPLLLQVGFGLTAFHAGSLMLAFAFGNLGMKMFTTRILDRFGFR
TTAAVNAGLGVLFVLLAGAMVPATPVAILLLVLLGCGVTRSMQYTLLATLGYADIGNREKGAASTLLSVTRQICIGLGVA
FAALTLRAVATLRGEHPATDAFVPADFHWAFLIVAIPLALSIPRYLRMDRTAGAALRTAS
>Mature_459_residues
TLLPYIVAATFFMEYLDSTIIATALPSMARSFHVGPNEISLGMTAYMLTLAVFIPISGWAADRFGSRTVFASAVVVFTAA
SVLCGFAGSVDQFVAARVLQGLGGAMMVPVGRFIVVRNTETSRMIQAISTITWPAVVAPVVGPTVGGMIVTFATWHWIFF
INVPLGLMVLGVIFAVVPQQFGERRPMDWGGFVLGGLSLTAILLGTELASNTQASLVLAGALFVAGLLLGAATLRHAAGQ
DHPLLDFRLMRHPTFAVTVLAGTLSRFTIDAIPYLVPLLLQVGFGLTAFHAGSLMLAFAFGNLGMKMFTTRILDRFGFRT
TAAVNAGLGVLFVLLAGAMVPATPVAILLLVLLGCGVTRSMQYTLLATLGYADIGNREKGAASTLLSVTRQICIGLGVAF
AALTLRAVATLRGEHPATDAFVPADFHWAFLIVAIPLALSIPRYLRMDRTAGAALRTAS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=458, Percent_Identity=35.589519650655, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI1788392, Length=437, Percent_Identity=32.7231121281465, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788710, Length=316, Percent_Identity=26.2658227848101, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1789042, Length=288, Percent_Identity=30.5555555555556, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081983, Length=426, Percent_Identity=27.2300469483568, Blast_Score=105, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6325455, Length=153, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=29.3333333333333, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=158, Percent_Identity=31.6455696202532, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48642; Mature: 48511

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLPYIVAATFFMEYLDSTIIATALPSMARSFHVGPNEISLGMTAYMLTLAVFIPISGW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
AADRFGSRTVFASAVVVFTAASVLCGFAGSVDQFVAARVLQGLGGAMMVPVGRFIVVRNT
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCEEEEECC
ETSRMIQAISTITWPAVVAPVVGPTVGGMIVTFATWHWIFFINVPLGLMVLGVIFAVVPQ
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHH
QFGERRPMDWGGFVLGGLSLTAILLGTELASNTQASLVLAGALFVAGLLLGAATLRHAAG
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QDHPLLDFRLMRHPTFAVTVLAGTLSRFTIDAIPYLVPLLLQVGFGLTAFHAGSLMLAFA
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FGNLGMKMFTTRILDRFGFRTTAAVNAGLGVLFVLLAGAMVPATPVAILLLVLLGCGVTR
HHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
SMQYTLLATLGYADIGNREKGAASTLLSVTRQICIGLGVAFAALTLRAVATLRGEHPATD
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AFVPADFHWAFLIVAIPLALSIPRYLRMDRTAGAALRTAS
CCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TLLPYIVAATFFMEYLDSTIIATALPSMARSFHVGPNEISLGMTAYMLTLAVFIPISGW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
AADRFGSRTVFASAVVVFTAASVLCGFAGSVDQFVAARVLQGLGGAMMVPVGRFIVVRNT
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCEEEEECC
ETSRMIQAISTITWPAVVAPVVGPTVGGMIVTFATWHWIFFINVPLGLMVLGVIFAVVPQ
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHH
QFGERRPMDWGGFVLGGLSLTAILLGTELASNTQASLVLAGALFVAGLLLGAATLRHAAG
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QDHPLLDFRLMRHPTFAVTVLAGTLSRFTIDAIPYLVPLLLQVGFGLTAFHAGSLMLAFA
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FGNLGMKMFTTRILDRFGFRTTAAVNAGLGVLFVLLAGAMVPATPVAILLLVLLGCGVTR
HHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
SMQYTLLATLGYADIGNREKGAASTLLSVTRQICIGLGVAFAALTLRAVATLRGEHPATD
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AFVPADFHWAFLIVAIPLALSIPRYLRMDRTAGAALRTAS
CCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]