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The map label for this gene is 162149020

Identifier: 162149020

GI number: 162149020

Start: 3340411

End: 3341823

Strand: Reverse

Name: 162149020

Synonym: GDI_3250

Alternate gene names: NA

Gene position: 3341823-3340411 (Counterclockwise)

Preceding gene: 162149021

Following gene: 162149019

Centisome position: 84.73

GC content: 70.28

Gene sequence:

>1413_bases
ATGTCGAACCCCGGCGAGCAAGATACGACCCCCATTGAATCGGTCAGCCAACTGGCCCGCCTGCTGGCGGACGGGGGCAA
GCCGCGCGGCCTGTGGCGGATCGGCACCGAGCACGAGAAATTCGGCTTCGTCCTGCCCGCCGCCGCCGGCCCGGCGCGCC
CGGCCTGGATGCCGCCGCCCTACGCCCCCGACGGCATCGCCGAAATCCTGACCGACCTGAACCGCCAGGATGGCGGCACG
CGGTGGGAGGCGATCCATGATGCGGGCAACCTGATCGGGCTGAAGGGCATCGGGCCCGAGCGCGGCGGGTCGGTGTCGCT
GGAGCCGGCGGGGCAGTTCGAACTGTCGGGCGCGCCGCTGGCCAGCCTGCACGATACGGGGCACGAGATGGCCCGGCATT
TCGAGGCGATCCGTCCGATTTCCCGGTCGCTGGGGGTCGGCTTCGCCCCGCTGGGCTTCCACCCGCTGGCGCGGCGCGCC
GACATGCCGTGGATGCCCAAGAGCCGCTACGCCATCATGCGGAACTACATGCCCAAGGTCGGCACGCTGGGCCTGGACAT
GATGCAGCGGACCTGCACCGTGCAGGTCAACCTGGATTTCGCATCCGAAGCCGACATGCTGCGCAAGCTGCGCGTGTCGC
TGGCGCTGCAGCCGGTGACGACGGCGCTGTTCGCCAATTCCCCGTTCCACGAGGGGCGGCCGAACGGGTTCATGTCCAAC
CGTGCCCGGATCTGGACCGATACCGACACTGCGCGGTCGGGCATGCCGGACCTGTTCTTCTCGCCCTCCTTCGGGTTCGA
GCCCTATGTCGAATGGCTGCTGGACGTGCCGATGTATTTCGTCGTGCGCGACGGGCGCATCCTGGACGTGGCGGGGCGGT
CGTTCCGGGCGTGGATGAAGGGCGAGCGCCAGGACGGGCTGGAGGGCCTGACCCCCACCGTGGGCGATTTCGAGGACCAC
ATGACGACGGCCTTCACCGACGTGCGGCTGAAGCGGTTCCTGGAAATGCGCGGGGCGGATGCCGGGTCGCCCGAGATGAT
GCTGGCCATGTCCGCCCTGTGGGTGGGGCTGCTGTATGACGACGCGGCGCTGGCCGCCGCCGACGCGCTGGTGCGCGAGC
AGGATTGGGCGCACTACGTCGCCCTGCGCGCCGCCGTGCCACGGGCGGGGCTGGACGCGCCGTGGGTGGGCGGCACGCTG
CGCGAACTGACGGCGCGGATGGTCGAGATCGCGGTGCAGGGGCTGCGCGCGCGCGCGGTGACGGACGGGACCGGACGTGA
CGAGAGCCATCTGCTGGCCCCGCTGCAGGAGATCGCGGCCGGCGGCCCGACGCAGGCCGAACGGTGGCTGGAACGCTATC
ACGGGGCCTGGAAAGGCGACGTCACGCATATCTTCAGCGAAGCGGCGGTTTAA

Upstream 100 bases:

>100_bases
GCAGGGTCGCGCTGAGAGCGCAGCCTCAAAAGCCTTGATGCTTACGACCCGCACGGCCTCTGACCGATCCGTTGCGTTTT
TTAGGATTTTTGCAGAAACG

Downstream 100 bases:

>100_bases
GAGTATCGGGCCTTCGTTTCGTTGAATTCGATAACGCGAATAATTCGCACGATATCGAAATGACTTGTGCGCATCAGCGA
TGTTCGACATACAGGCTGGA

Product: glutamate-cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSNPGEQDTTPIESVSQLARLLADGGKPRGLWRIGTEHEKFGFVLPAAAGPARPAWMPPPYAPDGIAEILTDLNRQDGGT
RWEAIHDAGNLIGLKGIGPERGGSVSLEPAGQFELSGAPLASLHDTGHEMARHFEAIRPISRSLGVGFAPLGFHPLARRA
DMPWMPKSRYAIMRNYMPKVGTLGLDMMQRTCTVQVNLDFASEADMLRKLRVSLALQPVTTALFANSPFHEGRPNGFMSN
RARIWTDTDTARSGMPDLFFSPSFGFEPYVEWLLDVPMYFVVRDGRILDVAGRSFRAWMKGERQDGLEGLTPTVGDFEDH
MTTAFTDVRLKRFLEMRGADAGSPEMMLAMSALWVGLLYDDAALAAADALVREQDWAHYVALRAAVPRAGLDAPWVGGTL
RELTARMVEIAVQGLRARAVTDGTGRDESHLLAPLQEIAAGGPTQAERWLERYHGAWKGDVTHIFSEAAV

Sequences:

>Translated_470_residues
MSNPGEQDTTPIESVSQLARLLADGGKPRGLWRIGTEHEKFGFVLPAAAGPARPAWMPPPYAPDGIAEILTDLNRQDGGT
RWEAIHDAGNLIGLKGIGPERGGSVSLEPAGQFELSGAPLASLHDTGHEMARHFEAIRPISRSLGVGFAPLGFHPLARRA
DMPWMPKSRYAIMRNYMPKVGTLGLDMMQRTCTVQVNLDFASEADMLRKLRVSLALQPVTTALFANSPFHEGRPNGFMSN
RARIWTDTDTARSGMPDLFFSPSFGFEPYVEWLLDVPMYFVVRDGRILDVAGRSFRAWMKGERQDGLEGLTPTVGDFEDH
MTTAFTDVRLKRFLEMRGADAGSPEMMLAMSALWVGLLYDDAALAAADALVREQDWAHYVALRAAVPRAGLDAPWVGGTL
RELTARMVEIAVQGLRARAVTDGTGRDESHLLAPLQEIAAGGPTQAERWLERYHGAWKGDVTHIFSEAAV
>Mature_469_residues
SNPGEQDTTPIESVSQLARLLADGGKPRGLWRIGTEHEKFGFVLPAAAGPARPAWMPPPYAPDGIAEILTDLNRQDGGTR
WEAIHDAGNLIGLKGIGPERGGSVSLEPAGQFELSGAPLASLHDTGHEMARHFEAIRPISRSLGVGFAPLGFHPLARRAD
MPWMPKSRYAIMRNYMPKVGTLGLDMMQRTCTVQVNLDFASEADMLRKLRVSLALQPVTTALFANSPFHEGRPNGFMSNR
ARIWTDTDTARSGMPDLFFSPSFGFEPYVEWLLDVPMYFVVRDGRILDVAGRSFRAWMKGERQDGLEGLTPTVGDFEDHM
TTAFTDVRLKRFLEMRGADAGSPEMMLAMSALWVGLLYDDAALAAADALVREQDWAHYVALRAAVPRAGLDAPWVGGTLR
ELTARMVEIAVQGLRARAVTDGTGRDESHLLAPLQEIAAGGPTQAERWLERYHGAWKGDVTHIFSEAAV

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 51515; Mature: 51384

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPGEQDTTPIESVSQLARLLADGGKPRGLWRIGTEHEKFGFVLPAAAGPARPAWMPPP
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCC
YAPDGIAEILTDLNRQDGGTRWEAIHDAGNLIGLKGIGPERGGSVSLEPAGQFELSGAPL
CCCCHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECCCCCCCCCEEEECCCCCEEECCCCH
ASLHDTGHEMARHFEAIRPISRSLGVGFAPLGFHPLARRADMPWMPKSRYAIMRNYMPKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHCCCCH
GTLGLDMMQRTCTVQVNLDFASEADMLRKLRVSLALQPVTTALFANSPFHEGRPNGFMSN
HHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
RARIWTDTDTARSGMPDLFFSPSFGFEPYVEWLLDVPMYFVVRDGRILDVAGRSFRAWMK
CCEEEECCCHHHCCCCCCEECCCCCCHHHHHHHHHCCEEEEEECCEEEECCCCHHHHHHC
GERQDGLEGLTPTVGDFEDHMTTAFTDVRLKRFLEMRGADAGSPEMMLAMSALWVGLLYD
CCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
DAALAAADALVREQDWAHYVALRAAVPRAGLDAPWVGGTLRELTARMVEIAVQGLRARAV
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEE
TDGTGRDESHLLAPLQEIAAGGPTQAERWLERYHGAWKGDVTHIFSEAAV
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SNPGEQDTTPIESVSQLARLLADGGKPRGLWRIGTEHEKFGFVLPAAAGPARPAWMPPP
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCC
YAPDGIAEILTDLNRQDGGTRWEAIHDAGNLIGLKGIGPERGGSVSLEPAGQFELSGAPL
CCCCHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECCCCCCCCCEEEECCCCCEEECCCCH
ASLHDTGHEMARHFEAIRPISRSLGVGFAPLGFHPLARRADMPWMPKSRYAIMRNYMPKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHCCCCH
GTLGLDMMQRTCTVQVNLDFASEADMLRKLRVSLALQPVTTALFANSPFHEGRPNGFMSN
HHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
RARIWTDTDTARSGMPDLFFSPSFGFEPYVEWLLDVPMYFVVRDGRILDVAGRSFRAWMK
CCEEEECCCHHHCCCCCCEECCCCCCHHHHHHHHHCCEEEEEECCEEEECCCCHHHHHHC
GERQDGLEGLTPTVGDFEDHMTTAFTDVRLKRFLEMRGADAGSPEMMLAMSALWVGLLYD
CCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
DAALAAADALVREQDWAHYVALRAAVPRAGLDAPWVGGTLRELTARMVEIAVQGLRARAV
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEE
TDGTGRDESHLLAPLQEIAAGGPTQAERWLERYHGAWKGDVTHIFSEAAV
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA