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The map label for this gene is pncA [H]

Identifier: 162148978

GI number: 162148978

Start: 3299360

End: 3299872

Strand: Direct

Name: pncA [H]

Synonym: GDI_3208

Alternate gene names: 162148978

Gene position: 3299360-3299872 (Clockwise)

Preceding gene: 162148972

Following gene: 162148979

Centisome position: 83.65

GC content: 68.42

Gene sequence:

>513_bases
GTGCCCGAGGGGGACGCCGTCATTCCCGTCATCAACCACCTGACGCGCCTGCCCTTCGGCCAGCAGGCGGCCAGCCAGGA
CTGGCACCCGCACGGTCATGCATCCTTCGCCACCGCCCAGCCGCCCGGCCCCTGGCGACAGCATTGCGTCGCCGGCACTC
CGGGCGCGGAGCTGGCGGCGTCGCTGGACCAGACGCGGATCGGCCTGGTGATCCGCAAGGGCCGCGCGCAGGACGTCGAC
AGCTATTCCGCCTTCCTCGACAACGACCGGACCACCCGTACGGGGCTGGAGGACTGGCTGAGGGGGCTGGGGATCACGCG
AATCTTCATCGCCGGCCTGGCGCTGGATTATTGCGTGGCCTTCACCGCCATCGACGCGAAAGCCCTTGGTTTTGAAACGA
TCGTGGTCGAAGATGCCTGCCGTGGGATCGATCCCGACCCCGACGCCACATGGCAGGCCCTGGCGCGGCACGATATCCTG
CATGTCCGGTCCGCCGAACTGGCGGGACAGTAA

Upstream 100 bases:

>100_bases
CCCCGCGCAAGGAGGCCCGACCGTGATCCACGTGACCGCGCATGATGCCCTTCTGGTCATCGACCTGCAGAACGATTTCC
TGCCCGGCGGCGCGCTGCCG

Downstream 100 bases:

>100_bases
GGGGGGGATCGACCATACCGGGGGAACGCCCGGCGCCGGGTGGGACCGGCCCGGGCATCGAGTGGGGGAAGACGAAGACA
TGACCGTTCCAGGTCAGGTA

Product: pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MPEGDAVIPVINHLTRLPFGQQAASQDWHPHGHASFATAQPPGPWRQHCVAGTPGAELAASLDQTRIGLVIRKGRAQDVD
SYSAFLDNDRTTRTGLEDWLRGLGITRIFIAGLALDYCVAFTAIDAKALGFETIVVEDACRGIDPDPDATWQALARHDIL
HVRSAELAGQ

Sequences:

>Translated_170_residues
MPEGDAVIPVINHLTRLPFGQQAASQDWHPHGHASFATAQPPGPWRQHCVAGTPGAELAASLDQTRIGLVIRKGRAQDVD
SYSAFLDNDRTTRTGLEDWLRGLGITRIFIAGLALDYCVAFTAIDAKALGFETIVVEDACRGIDPDPDATWQALARHDIL
HVRSAELAGQ
>Mature_169_residues
PEGDAVIPVINHLTRLPFGQQAASQDWHPHGHASFATAQPPGPWRQHCVAGTPGAELAASLDQTRIGLVIRKGRAQDVDS
YSAFLDNDRTTRTGLEDWLRGLGITRIFIAGLALDYCVAFTAIDAKALGFETIVVEDACRGIDPDPDATWQALARHDILH
VRSAELAGQ

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=163, Percent_Identity=42.3312883435583, Blast_Score=124, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17543130, Length=172, Percent_Identity=29.6511627906977, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193206735, Length=168, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321401, Length=177, Percent_Identity=31.638418079096, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648247, Length=185, Percent_Identity=33.5135135135135, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 18388; Mature: 18257

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGDAVIPVINHLTRLPFGQQAASQDWHPHGHASFATAQPPGPWRQHCVAGTPGAELAA
CCCCCEEHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHH
SLDQTRIGLVIRKGRAQDVDSYSAFLDNDRTTRTGLEDWLRGLGITRIFIAGLALDYCVA
HHHHHHEEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FTAIDAKALGFETIVVEDACRGIDPDPDATWQALARHDILHVRSAELAGQ
HHHHHHHHHCHHHEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PEGDAVIPVINHLTRLPFGQQAASQDWHPHGHASFATAQPPGPWRQHCVAGTPGAELAA
CCCCEEHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHH
SLDQTRIGLVIRKGRAQDVDSYSAFLDNDRTTRTGLEDWLRGLGITRIFIAGLALDYCVA
HHHHHHEEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FTAIDAKALGFETIVVEDACRGIDPDPDATWQALARHDILHVRSAELAGQ
HHHHHHHHHCHHHEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]