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The map label for this gene is ligA

Identifier: 162148970

GI number: 162148970

Start: 3291986

End: 3294073

Strand: Direct

Name: ligA

Synonym: GDI_3200

Alternate gene names: 162148970

Gene position: 3291986-3294073 (Clockwise)

Preceding gene: 162148969

Following gene: 162148971

Centisome position: 83.46

GC content: 69.64

Gene sequence:

>2088_bases
CTGGACCCGTCCGAACTGACCGAACCCCAGGCCATGGTGGAACTGGAGCGTCTGGCCCAGCAGATCCGCGCCCTGGACCG
CGCCTATTACGAGGACGACGCCCCCACCGTCACCGACGCCGAATATGACGCGCTACGCCAGCGCAACCTGGCGATCGAGG
CCCGCTTCCCCGACCTGCGCCGCGCGGACAGCCCTTCGCTGCACGTCAGCGGCGCGCCCTCCGCCGCGTTCGGCCGGCAT
CGCCACCTGGTGCCGATGCTCTCGCTGGACAACGTGTTCGGCCGCGAGGATTTCGAATCCTTCGTGACCCGCGCCGCCCG
CTTCCTCGGCCTGAACGACGATCAGGCCCGCGCCCTGCGCTTCGTCGCGGAACCCAAGATCGACGGCCTGTCGATCAGCC
TGACCTACGAGCACGGCCGCTTCGTGCGCGGCACCACGCGCGGCGACGGGACGGAGGGCGAGGACGTCACCGCCAACCTG
CGCACGCTGCGCGACGTGCCGCTGCGGCTGAAGGGCCCGGCCCCCGCGCTGATCGAAATCAGGGGCGAGGTCTTCCTGTC
CAAGCCCGCCTTCCTGTCGATCAACGCCGCCCAGGCCGAAGCCGGACAGAAACCCTTCGCCAATCCGCGCAATGCGGCGG
CGGGGTCGCTGCGCCAGCTCGATCCCAACATCACCGCCCGCCGGCCGCTGTCGCTGTTCGCCTATGCCCAGGGGTTTTCG
TCGGACCGGGTGGCCGACACCCACTGGGATTATCTGGAGCGGCTGCGGCAGTGGGGATTCACGGTCAATCCACTGTCCTG
CGTCGTCGAAAGCGCCGAGGCGGCGGAAGCGTTCATGGATCGCATCGCCCGCGAACGCTCGGGGCTGGAATACGATATCG
ACGGCGTGGTCTATAAGGTCGACGACCTGGCCCTGCAGGACCGGCTGGGCTTCGTCGGCCGGGCGCCGCGCTGGGCGATC
GCGTGGAAGTTCCCCGCCGAACAGGCCATCACCCGCCTGACCCGGATCGACATCCAGGTCGGCCGCACGGGCGCGCTGAC
CCCGGTGGCGATCCTGGAACCGGTCAATGTCGGCGGCGTCATCGTCACCCGTGCGACGTTGCACAACGAGGACGAGATCG
CCCGCAAGGACGTCCGCGTCGGCGACCTGGTCCAGATCCAGCGCGCGGGCGACGTCATTCCGCAGATCCTGGGCGTGGTG
CCGCCCGGCGCGGATGCCCCGCCGCGCGGCGAGGCATTCATCTTCCCCCACACCTGCCCGATCTGCGGCGCCCGCGCCGA
GCGGCCGCCGGGCGAGGTGGTGTGGCGCTGTACCGGCGGCCTGACCTGCCCGGCGCAGGTCGTCGAACGGCTGATCCATT
TCGTCTCGCGCGACGCGTTCGACATCGACGGGCTGGGCGAACGCACGATCACCGAATTCCATGCCGACGGGCTGCTGAAG
ACCCCGGCCGACATCTTCCGCCTGCCGGACCACGAGGCCGACATCGCGACGCGCGAGGGCTGGGGCACGGTCTCGGCCCG
CAACCTGACCGCGTCGGTCCGCGCTCGCCAGACCATCCCGCTGGCGCGCTTCATCTACGCGCTGGGCATCCGCCGCATCG
GCACCAGCAATGCACGCCTGCTGGCCCGGCATTACGGATCGTACACGCATTGGCGCGAACAGATGCTGCGCGCCACCACC
ATCGGGTCCGACGAACGGCTGGCGCTGGGCTCGATCACCGGGATCGGCGGCGCCATCGCGGACGAACTGGCCGCCTTCTT
CATGGAGGCGCACAATCTGGAAACCCTGGACGACCTGACGGCGATGCTGACGGCGATCGAGGACGAGGACCTGCCGGCCC
AGGGCCATCTGTCGGGCAAGACCGTCGTCTTCACCGGCACACTGACGACCATGACCCGGCCCGAGGCCAAGGCCATCGCC
GAACGGCTGGGCGCCCGCGTCACCGACAGCGTCTCGAAGAAGACCGATCTGGTGGTGCTGGGCGCCGATGCGGGCTCCAA
GGCCCGCAAGGCCGCCGAACTGGGAATCGACACGCTGGATGAGGAAGGCTGGCGCGAACTGGCCGGTATCGGCCCTGTCG
GTCCGTAA

Upstream 100 bases:

>100_bases
GACACGATCACCGAGGCCGCGCGGGCGGCGGCGGACAGTCTTCTGCAAGGTTAATATCGCCATGTCCCGCGCACACGATC
CCGCCCCCGATCTCGCCAGG

Downstream 100 bases:

>100_bases
ACGCGCTCTGGTGGCGATCCGACGCGCGTCCGCTGCGCTCCGGTGCTCACGGCCATCAAGGCCGCTCCGCTCCGGTGCTC
GCAGACACACGCCGGACGTG

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 695; Mature: 695

Protein sequence:

>695_residues
MDPSELTEPQAMVELERLAQQIRALDRAYYEDDAPTVTDAEYDALRQRNLAIEARFPDLRRADSPSLHVSGAPSAAFGRH
RHLVPMLSLDNVFGREDFESFVTRAARFLGLNDDQARALRFVAEPKIDGLSISLTYEHGRFVRGTTRGDGTEGEDVTANL
RTLRDVPLRLKGPAPALIEIRGEVFLSKPAFLSINAAQAEAGQKPFANPRNAAAGSLRQLDPNITARRPLSLFAYAQGFS
SDRVADTHWDYLERLRQWGFTVNPLSCVVESAEAAEAFMDRIARERSGLEYDIDGVVYKVDDLALQDRLGFVGRAPRWAI
AWKFPAEQAITRLTRIDIQVGRTGALTPVAILEPVNVGGVIVTRATLHNEDEIARKDVRVGDLVQIQRAGDVIPQILGVV
PPGADAPPRGEAFIFPHTCPICGARAERPPGEVVWRCTGGLTCPAQVVERLIHFVSRDAFDIDGLGERTITEFHADGLLK
TPADIFRLPDHEADIATREGWGTVSARNLTASVRARQTIPLARFIYALGIRRIGTSNARLLARHYGSYTHWREQMLRATT
IGSDERLALGSITGIGGAIADELAAFFMEAHNLETLDDLTAMLTAIEDEDLPAQGHLSGKTVVFTGTLTTMTRPEAKAIA
ERLGARVTDSVSKKTDLVVLGADAGSKARKAAELGIDTLDEEGWRELAGIGPVGP

Sequences:

>Translated_695_residues
MDPSELTEPQAMVELERLAQQIRALDRAYYEDDAPTVTDAEYDALRQRNLAIEARFPDLRRADSPSLHVSGAPSAAFGRH
RHLVPMLSLDNVFGREDFESFVTRAARFLGLNDDQARALRFVAEPKIDGLSISLTYEHGRFVRGTTRGDGTEGEDVTANL
RTLRDVPLRLKGPAPALIEIRGEVFLSKPAFLSINAAQAEAGQKPFANPRNAAAGSLRQLDPNITARRPLSLFAYAQGFS
SDRVADTHWDYLERLRQWGFTVNPLSCVVESAEAAEAFMDRIARERSGLEYDIDGVVYKVDDLALQDRLGFVGRAPRWAI
AWKFPAEQAITRLTRIDIQVGRTGALTPVAILEPVNVGGVIVTRATLHNEDEIARKDVRVGDLVQIQRAGDVIPQILGVV
PPGADAPPRGEAFIFPHTCPICGARAERPPGEVVWRCTGGLTCPAQVVERLIHFVSRDAFDIDGLGERTITEFHADGLLK
TPADIFRLPDHEADIATREGWGTVSARNLTASVRARQTIPLARFIYALGIRRIGTSNARLLARHYGSYTHWREQMLRATT
IGSDERLALGSITGIGGAIADELAAFFMEAHNLETLDDLTAMLTAIEDEDLPAQGHLSGKTVVFTGTLTTMTRPEAKAIA
ERLGARVTDSVSKKTDLVVLGADAGSKARKAAELGIDTLDEEGWRELAGIGPVGP
>Mature_695_residues
MDPSELTEPQAMVELERLAQQIRALDRAYYEDDAPTVTDAEYDALRQRNLAIEARFPDLRRADSPSLHVSGAPSAAFGRH
RHLVPMLSLDNVFGREDFESFVTRAARFLGLNDDQARALRFVAEPKIDGLSISLTYEHGRFVRGTTRGDGTEGEDVTANL
RTLRDVPLRLKGPAPALIEIRGEVFLSKPAFLSINAAQAEAGQKPFANPRNAAAGSLRQLDPNITARRPLSLFAYAQGFS
SDRVADTHWDYLERLRQWGFTVNPLSCVVESAEAAEAFMDRIARERSGLEYDIDGVVYKVDDLALQDRLGFVGRAPRWAI
AWKFPAEQAITRLTRIDIQVGRTGALTPVAILEPVNVGGVIVTRATLHNEDEIARKDVRVGDLVQIQRAGDVIPQILGVV
PPGADAPPRGEAFIFPHTCPICGARAERPPGEVVWRCTGGLTCPAQVVERLIHFVSRDAFDIDGLGERTITEFHADGLLK
TPADIFRLPDHEADIATREGWGTVSARNLTASVRARQTIPLARFIYALGIRRIGTSNARLLARHYGSYTHWREQMLRATT
IGSDERLALGSITGIGGAIADELAAFFMEAHNLETLDDLTAMLTAIEDEDLPAQGHLSGKTVVFTGTLTTMTRPEAKAIA
ERLGARVTDSVSKKTDLVVLGADAGSKARKAAELGIDTLDEEGWRELAGIGPVGP

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=686, Percent_Identity=45.33527696793, Blast_Score=550, Evalue=1e-157,
Organism=Escherichia coli, GI87082305, Length=539, Percent_Identity=24.860853432282, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324791, Length=72, Percent_Identity=51.3888888888889, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_GLUDA (A9H0L6)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001603431.1
- RefSeq:   YP_002277505.1
- ProteinModelPortal:   A9H0L6
- GeneID:   5790617
- GeneID:   6976598
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_3160
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK935936
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75947; Mature: 75947

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 126-126 BINDING 124-124 BINDING 147-147 BINDING 183-183 BINDING 299-299 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPSELTEPQAMVELERLAQQIRALDRAYYEDDAPTVTDAEYDALRQRNLAIEARFPDLR
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEEEECCCCCC
RADSPSLHVSGAPSAAFGRHRHLVPMLSLDNVFGREDFESFVTRAARFLGLNDDQARALR
CCCCCCEEECCCCCHHHCCCCCEEEEECHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHH
FVAEPKIDGLSISLTYEHGRFVRGTTRGDGTEGEDVTANLRTLRDVPLRLKGPAPALIEI
HHCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHCCCEEECCCCCEEEEE
RGEVFLSKPAFLSINAAQAEAGQKPFANPRNAAAGSLRQLDPNITARRPLSLFAYAQGFS
ECEEEECCCCEEEEEHHHHHCCCCCCCCCCCHHCCCHHHCCCCCCCCCCHHHHHHHCCCC
SDRVADTHWDYLERLRQWGFTVNPLSCVVESAEAAEAFMDRIARERSGLEYDIDGVVYKV
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEE
DDLALQDRLGFVGRAPRWAIAWKFPAEQAITRLTRIDIQVGRTGALTPVAILEPVNVGGV
HHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHEEEECCCCCCCCCCEECCCCCCEE
IVTRATLHNEDEIARKDVRVGDLVQIQRAGDVIPQILGVVPPGADAPPRGEAFIFPHTCP
EEEEEECCCCHHHHHHCCCCCHHEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCC
ICGARAERPPGEVVWRCTGGLTCPAQVVERLIHFVSRDAFDIDGLGERTITEFHADGLLK
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCC
TPADIFRLPDHEADIATREGWGTVSARNLTASVRARQTIPLARFIYALGIRRIGTSNARL
CCHHHHCCCCCCCCCCCCCCCCCEECCCEEHHHHHHHCCHHHHHHHHHHHHHCCCCCHHH
LARHYGSYTHWREQMLRATTIGSDERLALGSITGIGGAIADELAAFFMEAHNLETLDDLT
HHHHHCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
AMLTAIEDEDLPAQGHLSGKTVVFTGTLTTMTRPEAKAIAERLGARVTDSVSKKTDLVVL
HHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHCCHHHHHHCCCCCEEEE
GADAGSKARKAAELGIDTLDEEGWRELAGIGPVGP
ECCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDPSELTEPQAMVELERLAQQIRALDRAYYEDDAPTVTDAEYDALRQRNLAIEARFPDLR
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEEEECCCCCC
RADSPSLHVSGAPSAAFGRHRHLVPMLSLDNVFGREDFESFVTRAARFLGLNDDQARALR
CCCCCCEEECCCCCHHHCCCCCEEEEECHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHH
FVAEPKIDGLSISLTYEHGRFVRGTTRGDGTEGEDVTANLRTLRDVPLRLKGPAPALIEI
HHCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHCCCEEECCCCCEEEEE
RGEVFLSKPAFLSINAAQAEAGQKPFANPRNAAAGSLRQLDPNITARRPLSLFAYAQGFS
ECEEEECCCCEEEEEHHHHHCCCCCCCCCCCHHCCCHHHCCCCCCCCCCHHHHHHHCCCC
SDRVADTHWDYLERLRQWGFTVNPLSCVVESAEAAEAFMDRIARERSGLEYDIDGVVYKV
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEE
DDLALQDRLGFVGRAPRWAIAWKFPAEQAITRLTRIDIQVGRTGALTPVAILEPVNVGGV
HHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHEEEECCCCCCCCCCEECCCCCCEE
IVTRATLHNEDEIARKDVRVGDLVQIQRAGDVIPQILGVVPPGADAPPRGEAFIFPHTCP
EEEEEECCCCHHHHHHCCCCCHHEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCC
ICGARAERPPGEVVWRCTGGLTCPAQVVERLIHFVSRDAFDIDGLGERTITEFHADGLLK
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCC
TPADIFRLPDHEADIATREGWGTVSARNLTASVRARQTIPLARFIYALGIRRIGTSNARL
CCHHHHCCCCCCCCCCCCCCCCCEECCCEEHHHHHHHCCHHHHHHHHHHHHHCCCCCHHH
LARHYGSYTHWREQMLRATTIGSDERLALGSITGIGGAIADELAAFFMEAHNLETLDDLT
HHHHHCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
AMLTAIEDEDLPAQGHLSGKTVVFTGTLTTMTRPEAKAIAERLGARVTDSVSKKTDLVVL
HHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHCCHHHHHHCCCCCEEEE
GADAGSKARKAAELGIDTLDEEGWRELAGIGPVGP
ECCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA