Click here to switch to the map view.

The map label for this gene is murB [H]

Identifier: 162148963

GI number: 162148963

Start: 3281534

End: 3283471

Strand: Direct

Name: murB [H]

Synonym: GDI_3193

Alternate gene names: 162148963

Gene position: 3281534-3283471 (Clockwise)

Preceding gene: 162148962

Following gene: 162148964

Centisome position: 83.2

GC content: 72.65

Gene sequence:

>1938_bases
ATGACCACGCCCCCCGCCCCTTCGGATGCATCCTGGACCGCCGAGGTACGCGCAATGCTGGCCGGCCTGCGCGGACGGCT
GACCCAGGGTGCGCCGCTGGGCCCGCGCACCTGGTTCCGCGTCGGCGGCCCGGCGGAAATCCTGCTGCAGCCGGCCGATA
CGCAGGACCTGGCGGACGCGCTGCACCGCCTGCCGCCGGACGTGCCGGTCACGGTGCTGGGCGCGTGCTCGAACGTGATC
ATCCGCGACGGCGGGATCGACGGGGTGGTGATCCGGCTGGGCGGCGGCTTTGCCGACATCGTGGCGGAACCGGACGGGCT
GGTGGTCGGCGCGGCCTGCCTGGACATGGTCGTGGCCGAACGCGCGGCCGAGGCGGGGCTGAAGGGGCTGGAATTCCTGG
CCGGCATTCCCGGTTCGATCGGCGGCGCGGTCGCCATGAACGCGGGCGCCCACGGGTCCGACGTCGCGACGGTGCTGGAC
TGGGCCGACATCATCACCCGCGACGGCAACAGCGTGCGCCTGTCCGGGCCGGCGCTGGGGTTCGGCTATCGCCGGTCGGC
GCTGCCGGCTGGCGCGGTGGTGGTGCGCGCGCGCCTGCGCGCCGCGCCCGCGGCCCCCGCCGACATCCGCCAGGCCATCG
CGGCCATCCGCCAGTCGCGCGAGGAGTCGCAGCCCACGCGGGCCCGCACCGGCGGATCGACCTTCCGCAACCCCGACCCG
TCGGTGACGGACCGCAAGGCGTGGGAACTGATCGACAGCGCCGGCTGCCGGGGCCTGACCATGGACGGCGCACAGGTCAG
CACGAAGCACTGCAATTTCATCCTGAACACCGGCGACGCCACCGCCGCCGCCCCTGGAACGCCTGGGCGAGAGCGTGCGC
AAGCGGGTGCGCGTCCATACCGGCGTCACGCTGGAGTGGGAAATCAAGCGGATCGGCCGTCCCGCCACCCCATCCCCGAC
CGAGACAGCCATTGGGGCAGGAGAACCGTCATGACCCGTCCGCGCACCATCACGGTGCTGACCGGCGGCCTGTCCGCCGA
GCGCACCGTCAGCCTGTCCAGCGGCGCCGGCATCAGCGCCGCCCTGCGCGAGGAAGGATTCGACGTCCGCGTGCTGGATG
CCGGGCCGGACCTCGGCGCCATCGTCGCGGACCTGACGGCGCACCGGCCCGACGTGGTGTTCAACGCCCTGCACGGCCGC
TTCGGCGAGGACGGGTCAATCCAGGGCGTGCTGGACTGGATGAACATCCCCTACACCCATTCCGGCGTCCGCGCGTCGGC
CATGGCGATGGACAAGGCGGCGGCCCGCTCGGCCTTCCGCGCCGCGGGCCTGCCGGTGGCGCAGGGCGGGGTCATCGCCA
TCGCCGACTTTCCCGCCGCCGACCCGCTGCCCGCCCCCTATGTCATCAAGCCGCTGAACGAAGGATCGTCGGTGGGTGTC
GAGATCGTACGGCCGGGCACCAACCGCCGCGCCGCGATCGCCGACGCATGGACCTACGGCCCCCTGGCGCTGGTCGAGGA
ATTCATCCCCGGGCGCGAACTGACGGTGGGGGTAATGGGCGACCGCGCCCTGACCGTGACCGACATCACCCCCAATCCCG
ATGCCGGGCATGAATTCTACGACTACGCCGCCAAGTACCAGAATGGCGGATCGCGGCACATCCTGCCCGCGCGTATCCAC
CCGGACGCGTTCGCCCAGGCGCTGGACCTGGCGGTGGCCGCCCACCGCGCGCTGGGCTGCGCCGGCGCGTCGCGCACCGA
TTTCCGTTACGACGACACGCAGTGCGGCGACGGGCCGGGGCGACTGGTGATCCTGGAGGTCAATACCCAGCCCGGCATGA
CCCCGACCTCGCTGCTGCCCGAACAGGCGGGGGCCTGCGGCATCCCCTATGGCGCGCTGTGCCGGTGGATGGTCGAACAC
GCGGCGTGCCGGACCTAG

Upstream 100 bases:

>100_bases
GCCTGGGGGCCGGCAGCATCACCAACTGGGCGCAGGCCCTGCCCGGCCAACTGGCGGCGTTGCAGAACCCGGCCGCGGCG
CGAAAGGGCGAATGCGCGGC

Downstream 100 bases:

>100_bases
ATGCGACGGACGCGGGACAATCGAAGCGACCGGCCGTCACGGCTGTCGATCTTCATGCGGCGGCAACGCCGCATGGCGCG
GCCGGTCGCGCTTGCCCTCG

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 645; Mature: 644

Protein sequence:

>645_residues
MTTPPAPSDASWTAEVRAMLAGLRGRLTQGAPLGPRTWFRVGGPAEILLQPADTQDLADALHRLPPDVPVTVLGACSNVI
IRDGGIDGVVIRLGGGFADIVAEPDGLVVGAACLDMVVAERAAEAGLKGLEFLAGIPGSIGGAVAMNAGAHGSDVATVLD
WADIITRDGNSVRLSGPALGFGYRRSALPAGAVVVRARLRAAPAAPADIRQAIAAIRQSREESQPTRARTGGSTFRNPDP
SVTDRKAWELIDSAGCRGLTMDGAQVSTKHCNFILNTGDATAAAPGTPGRERAQAGARPYRRHAGVGNQADRPSRHPIPD
RDSHWGRRTVMTRPRTITVLTGGLSAERTVSLSSGAGISAALREEGFDVRVLDAGPDLGAIVADLTAHRPDVVFNALHGR
FGEDGSIQGVLDWMNIPYTHSGVRASAMAMDKAAARSAFRAAGLPVAQGGVIAIADFPAADPLPAPYVIKPLNEGSSVGV
EIVRPGTNRRAAIADAWTYGPLALVEEFIPGRELTVGVMGDRALTVTDITPNPDAGHEFYDYAAKYQNGGSRHILPARIH
PDAFAQALDLAVAAHRALGCAGASRTDFRYDDTQCGDGPGRLVILEVNTQPGMTPTSLLPEQAGACGIPYGALCRWMVEH
AACRT

Sequences:

>Translated_645_residues
MTTPPAPSDASWTAEVRAMLAGLRGRLTQGAPLGPRTWFRVGGPAEILLQPADTQDLADALHRLPPDVPVTVLGACSNVI
IRDGGIDGVVIRLGGGFADIVAEPDGLVVGAACLDMVVAERAAEAGLKGLEFLAGIPGSIGGAVAMNAGAHGSDVATVLD
WADIITRDGNSVRLSGPALGFGYRRSALPAGAVVVRARLRAAPAAPADIRQAIAAIRQSREESQPTRARTGGSTFRNPDP
SVTDRKAWELIDSAGCRGLTMDGAQVSTKHCNFILNTGDATAAAPGTPGRERAQAGARPYRRHAGVGNQADRPSRHPIPD
RDSHWGRRTVMTRPRTITVLTGGLSAERTVSLSSGAGISAALREEGFDVRVLDAGPDLGAIVADLTAHRPDVVFNALHGR
FGEDGSIQGVLDWMNIPYTHSGVRASAMAMDKAAARSAFRAAGLPVAQGGVIAIADFPAADPLPAPYVIKPLNEGSSVGV
EIVRPGTNRRAAIADAWTYGPLALVEEFIPGRELTVGVMGDRALTVTDITPNPDAGHEFYDYAAKYQNGGSRHILPARIH
PDAFAQALDLAVAAHRALGCAGASRTDFRYDDTQCGDGPGRLVILEVNTQPGMTPTSLLPEQAGACGIPYGALCRWMVEH
AACRT
>Mature_644_residues
TTPPAPSDASWTAEVRAMLAGLRGRLTQGAPLGPRTWFRVGGPAEILLQPADTQDLADALHRLPPDVPVTVLGACSNVII
RDGGIDGVVIRLGGGFADIVAEPDGLVVGAACLDMVVAERAAEAGLKGLEFLAGIPGSIGGAVAMNAGAHGSDVATVLDW
ADIITRDGNSVRLSGPALGFGYRRSALPAGAVVVRARLRAAPAAPADIRQAIAAIRQSREESQPTRARTGGSTFRNPDPS
VTDRKAWELIDSAGCRGLTMDGAQVSTKHCNFILNTGDATAAAPGTPGRERAQAGARPYRRHAGVGNQADRPSRHPIPDR
DSHWGRRTVMTRPRTITVLTGGLSAERTVSLSSGAGISAALREEGFDVRVLDAGPDLGAIVADLTAHRPDVVFNALHGRF
GEDGSIQGVLDWMNIPYTHSGVRASAMAMDKAAARSAFRAAGLPVAQGGVIAIADFPAADPLPAPYVIKPLNEGSSVGVE
IVRPGTNRRAAIADAWTYGPLALVEEFIPGRELTVGVMGDRALTVTDITPNPDAGHEFYDYAAKYQNGGSRHILPARIHP
DAFAQALDLAVAAHRALGCAGASRTDFRYDDTQCGDGPGRLVILEVNTQPGMTPTSLLPEQAGACGIPYGALCRWMVEHA
ACRT

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=314, Percent_Identity=37.5796178343949, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1786579, Length=358, Percent_Identity=32.4022346368715, Blast_Score=137, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 67297; Mature: 67166

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPPAPSDASWTAEVRAMLAGLRGRLTQGAPLGPRTWFRVGGPAEILLQPADTQDLADA
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEECCCHHHHHHH
LHRLPPDVPVTVLGACSNVIIRDGGIDGVVIRLGGGFADIVAEPDGLVVGAACLDMVVAE
HHHCCCCCCEEEEECCCCEEEECCCCCEEEEEECCCEEEEEECCCCEEEHHHHHHHHHHH
RAAEAGLKGLEFLAGIPGSIGGAVAMNAGAHGSDVATVLDWADIITRDGNSVRLSGPALG
HHHHHCCHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCC
FGYRRSALPAGAVVVRARLRAAPAAPADIRQAIAAIRQSREESQPTRARTGGSTFRNPDP
CCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCC
SVTDRKAWELIDSAGCRGLTMDGAQVSTKHCNFILNTGDATAAAPGTPGRERAQAGARPY
CCCCHHHHHHHHHCCCCCEEECCCEEECCCCEEEEECCCCCCCCCCCCCHHHHHHCCCHH
RRHAGVGNQADRPSRHPIPDRDSHWGRRTVMTRPRTITVLTGGLSAERTVSLSSGAGISA
HHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEECCCCCCHH
ALREEGFDVRVLDAGPDLGAIVADLTAHRPDVVFNALHGRFGEDGSIQGVLDWMNIPYTH
HHHHCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
SGVRASAMAMDKAAARSAFRAAGLPVAQGGVIAIADFPAADPLPAPYVIKPLNEGSSVGV
CCCHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCCEEEEECCCCCCCCE
EIVRPGTNRRAAIADAWTYGPLALVEEFIPGRELTVGVMGDRALTVTDITPNPDAGHEFY
EEECCCCCCCEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCCHHH
DYAAKYQNGGSRHILPARIHPDAFAQALDLAVAAHRALGCAGASRTDFRYDDTQCGDGPG
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
RLVILEVNTQPGMTPTSLLPEQAGACGIPYGALCRWMVEHAACRT
EEEEEEECCCCCCCHHHHCHHHCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTPPAPSDASWTAEVRAMLAGLRGRLTQGAPLGPRTWFRVGGPAEILLQPADTQDLADA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEECCCHHHHHHH
LHRLPPDVPVTVLGACSNVIIRDGGIDGVVIRLGGGFADIVAEPDGLVVGAACLDMVVAE
HHHCCCCCCEEEEECCCCEEEECCCCCEEEEEECCCEEEEEECCCCEEEHHHHHHHHHHH
RAAEAGLKGLEFLAGIPGSIGGAVAMNAGAHGSDVATVLDWADIITRDGNSVRLSGPALG
HHHHHCCHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCC
FGYRRSALPAGAVVVRARLRAAPAAPADIRQAIAAIRQSREESQPTRARTGGSTFRNPDP
CCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCC
SVTDRKAWELIDSAGCRGLTMDGAQVSTKHCNFILNTGDATAAAPGTPGRERAQAGARPY
CCCCHHHHHHHHHCCCCCEEECCCEEECCCCEEEEECCCCCCCCCCCCCHHHHHHCCCHH
RRHAGVGNQADRPSRHPIPDRDSHWGRRTVMTRPRTITVLTGGLSAERTVSLSSGAGISA
HHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEECCCCCCHH
ALREEGFDVRVLDAGPDLGAIVADLTAHRPDVVFNALHGRFGEDGSIQGVLDWMNIPYTH
HHHHCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
SGVRASAMAMDKAAARSAFRAAGLPVAQGGVIAIADFPAADPLPAPYVIKPLNEGSSVGV
CCCHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCCEEEEECCCCCCCCE
EIVRPGTNRRAAIADAWTYGPLALVEEFIPGRELTVGVMGDRALTVTDITPNPDAGHEFY
EEECCCCCCCEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCCHHH
DYAAKYQNGGSRHILPARIHPDAFAQALDLAVAAHRALGCAGASRTDFRYDDTQCGDGPG
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
RLVILEVNTQPGMTPTSLLPEQAGACGIPYGALCRWMVEHAACRT
EEEEEEECCCCCCCHHHHCHHHCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA