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The map label for this gene is murD [H]

Identifier: 162148959

GI number: 162148959

Start: 3276360

End: 3277757

Strand: Direct

Name: murD [H]

Synonym: GDI_3189

Alternate gene names: 162148959

Gene position: 3276360-3277757 (Clockwise)

Preceding gene: 162148958

Following gene: 162148960

Centisome position: 83.07

GC content: 74.61

Gene sequence:

>1398_bases
ATGAGCGGCCCGGGCCTGTTCCCCCCCGGCCTGTTGGCCGGCAGCCGCTACGCGGTGCTGGGGCTGGGGCGCAACGGCGC
CCCCGCCGTCCGCGCGCTGGCCGCGATGGGGGCGGACGTGCAGGCCTGGGATGACGGCGACGCCGCTCGTGCGGCGGTCG
CGGACGTGCCGGGCGTCGCCATCGCGCCGTTCGACACGCTGGCGGGGTTCGACGCGCTGGTGCTCTCGCCCGGCATTCCG
CACATCCTGCCCCACCCGCATCCTGTCGCCCGGATGGCGCACGCGGCGGGCGTTCCCATCCTGTCGGATGCCAACCTGCT
GTTCCAGGCGGTGCGCCGGTCCGGGTCGCGCGCGCGCTTCGCCGGCATCACCGGAACCAACGGCAAATCGACCACCACCA
CCCTGCTGGCCCACATGCTCCACACCGCCGGCCTGCCGGTCGCGGCGGGCGGGAACCTGGGCCCGGCGGCGCTGGCGCTG
CCGCTGCTGCCCGACACGGGCGTCTATGTGCTGGAAATGTCGTCCTACATGCTGGAACGGCTGCGGGACCTGCGCTTCGA
CGCCGCGTGCCTGCTGAACCTGACGCCCGACCACCTGGACCGCCATGGCGACATGGCAGGCTATCGACGGGCCAAGCAGC
ACGTCTTCGACCGCCAGGGACCGGGCGACCTGGCCGTGCTGGGCCAGTACGACGCCCCGGACGACGCGGCGCTGGCCCGC
GACCTGGCGGCGCGCGGCATCCGGGTCGTCACCATATCCGGCACCCGTGCCGACGCCGGCCTGACCGGCGCCGGGGGTGT
GCTGCGCGACGCGGACGGGCCGATCGCCACCCTGGCCGATGCCCCATCCCTGCCCGGCGCGCACAATGCCGAAAACGCGG
CGGCGGCGGCGGCGATGGCCCTGGCGCTGGGCGTGCCGCGCGCCACGATCGCCGGCGCGCTGCGGACCTTCCCGGGCCTG
GCCCACCGGCAGAAGCCGGTCGGCACCGTCGACGGCGTGCGCTTCATCGACGACAGCAAGGCGACGAACGCCGATGCCGC
ATCGCGGGCGCTGGGCTGCTACGACCGGCTGGTGTGGATCGCGGGCGGTGTGGCCAAGGCCGGCGGAATCGCGGACCTGG
CGCCGTTCTTCCCGCGCGTGGCCCATGCTTTCCTGATCGGGCAGGACGCGCCGGCGCTGGCCGAAACCCTGGCCGCGCAC
GGCGTTGCCTTCACGCTGTCGGGCACGATGGACGCGGCGGTGCCCGCCGCCCTCGCCCTGGCGCGGCGGGACGGCGTGCC
GGTGGTGCTGCTGTCCCCCGCCTGCGCCAGCTTTGATCAGTACGCCAGCTTCGAACATCGCGGCCGGCACTTCGCGGACC
TGGTCCGCCTGCTGGCGCCCACATTGGGGACGGACTGA

Upstream 100 bases:

>100_bases
ATTTCGAAAAGAAAGGCTGGCAGGAGCCCAAGATCGTCATCCGCTTCTGGATCGTGTCCATCGTCCTGGGACTGTGTGGC
CTGGCCACGCTGAAGCTGCG

Downstream 100 bases:

>100_bases
TGGCCGGGATCTCACGCGTCGATGCATCCTACCTTGCGCGCTGGTGGCGCAATGTCGACCGGGTGACGCTGTCCTGCGTC
GGCGTACTGATCGGCTTCGG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSGPGLFPPGLLAGSRYAVLGLGRNGAPAVRALAAMGADVQAWDDGDAARAAVADVPGVAIAPFDTLAGFDALVLSPGIP
HILPHPHPVARMAHAAGVPILSDANLLFQAVRRSGSRARFAGITGTNGKSTTTTLLAHMLHTAGLPVAAGGNLGPAALAL
PLLPDTGVYVLEMSSYMLERLRDLRFDAACLLNLTPDHLDRHGDMAGYRRAKQHVFDRQGPGDLAVLGQYDAPDDAALAR
DLAARGIRVVTISGTRADAGLTGAGGVLRDADGPIATLADAPSLPGAHNAENAAAAAAMALALGVPRATIAGALRTFPGL
AHRQKPVGTVDGVRFIDDSKATNADAASRALGCYDRLVWIAGGVAKAGGIADLAPFFPRVAHAFLIGQDAPALAETLAAH
GVAFTLSGTMDAAVPAALALARRDGVPVVLLSPACASFDQYASFEHRGRHFADLVRLLAPTLGTD

Sequences:

>Translated_465_residues
MSGPGLFPPGLLAGSRYAVLGLGRNGAPAVRALAAMGADVQAWDDGDAARAAVADVPGVAIAPFDTLAGFDALVLSPGIP
HILPHPHPVARMAHAAGVPILSDANLLFQAVRRSGSRARFAGITGTNGKSTTTTLLAHMLHTAGLPVAAGGNLGPAALAL
PLLPDTGVYVLEMSSYMLERLRDLRFDAACLLNLTPDHLDRHGDMAGYRRAKQHVFDRQGPGDLAVLGQYDAPDDAALAR
DLAARGIRVVTISGTRADAGLTGAGGVLRDADGPIATLADAPSLPGAHNAENAAAAAAMALALGVPRATIAGALRTFPGL
AHRQKPVGTVDGVRFIDDSKATNADAASRALGCYDRLVWIAGGVAKAGGIADLAPFFPRVAHAFLIGQDAPALAETLAAH
GVAFTLSGTMDAAVPAALALARRDGVPVVLLSPACASFDQYASFEHRGRHFADLVRLLAPTLGTD
>Mature_464_residues
SGPGLFPPGLLAGSRYAVLGLGRNGAPAVRALAAMGADVQAWDDGDAARAAVADVPGVAIAPFDTLAGFDALVLSPGIPH
ILPHPHPVARMAHAAGVPILSDANLLFQAVRRSGSRARFAGITGTNGKSTTTTLLAHMLHTAGLPVAAGGNLGPAALALP
LLPDTGVYVLEMSSYMLERLRDLRFDAACLLNLTPDHLDRHGDMAGYRRAKQHVFDRQGPGDLAVLGQYDAPDDAALARD
LAARGIRVVTISGTRADAGLTGAGGVLRDADGPIATLADAPSLPGAHNAENAAAAAAMALALGVPRATIAGALRTFPGLA
HRQKPVGTVDGVRFIDDSKATNADAASRALGCYDRLVWIAGGVAKAGGIADLAPFFPRVAHAFLIGQDAPALAETLAAHG
VAFTLSGTMDAAVPAALALARRDGVPVVLLSPACASFDQYASFEHRGRHFADLVRLLAPTLGTD

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=401, Percent_Identity=36.4089775561097, Blast_Score=155, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47456; Mature: 47324

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPGLFPPGLLAGSRYAVLGLGRNGAPAVRALAAMGADVQAWDDGDAARAAVADVPGVA
CCCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEECCCCCHHHHHHHCCCCCE
IAPFDTLAGFDALVLSPGIPHILPHPHPVARMAHAAGVPILSDANLLFQAVRRSGSRARF
ECCHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCEECCHHHHHHHHHCCCCCEEE
AGITGTNGKSTTTTLLAHMLHTAGLPVAAGGNLGPAALALPLLPDTGVYVLEMSSYMLER
EECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCHHHHEEECCCCCCEEEEHHHHHHHHH
LRDLRFDAACLLNLTPDHLDRHGDMAGYRRAKQHVFDRQGPGDLAVLGQYDAPDDAALAR
HHHCCCCCEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHH
DLAARGIRVVTISGTRADAGLTGAGGVLRDADGPIATLADAPSLPGAHNAENAAAAAAMA
HHHHCCEEEEEEECCCCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
LALGVPRATIAGALRTFPGLAHRQKPVGTVDGVRFIDDSKATNADAASRALGCYDRLVWI
HHHCCCHHHHHHHHHHCCCHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHH
AGGVAKAGGIADLAPFFPRVAHAFLIGQDAPALAETLAAHGVAFTLSGTMDAAVPAALAL
HCCHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHH
ARRDGVPVVLLSPACASFDQYASFEHRGRHFADLVRLLAPTLGTD
HHCCCCCEEEECCCHHCHHHHHHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGPGLFPPGLLAGSRYAVLGLGRNGAPAVRALAAMGADVQAWDDGDAARAAVADVPGVA
CCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEECCCCCHHHHHHHCCCCCE
IAPFDTLAGFDALVLSPGIPHILPHPHPVARMAHAAGVPILSDANLLFQAVRRSGSRARF
ECCHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCEECCHHHHHHHHHCCCCCEEE
AGITGTNGKSTTTTLLAHMLHTAGLPVAAGGNLGPAALALPLLPDTGVYVLEMSSYMLER
EECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCHHHHEEECCCCCCEEEEHHHHHHHHH
LRDLRFDAACLLNLTPDHLDRHGDMAGYRRAKQHVFDRQGPGDLAVLGQYDAPDDAALAR
HHHCCCCCEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHH
DLAARGIRVVTISGTRADAGLTGAGGVLRDADGPIATLADAPSLPGAHNAENAAAAAAMA
HHHHCCEEEEEEECCCCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
LALGVPRATIAGALRTFPGLAHRQKPVGTVDGVRFIDDSKATNADAASRALGCYDRLVWI
HHHCCCHHHHHHHHHHCCCHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHH
AGGVAKAGGIADLAPFFPRVAHAFLIGQDAPALAETLAAHGVAFTLSGTMDAAVPAALAL
HCCHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHH
ARRDGVPVVLLSPACASFDQYASFEHRGRHFADLVRLLAPTLGTD
HHCCCCCEEEECCCHHCHHHHHHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA