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The map label for this gene is trmD

Identifier: 162148860

GI number: 162148860

Start: 3184575

End: 3185288

Strand: Reverse

Name: trmD

Synonym: GDI_3089

Alternate gene names: 162148860

Gene position: 3185288-3184575 (Counterclockwise)

Preceding gene: 162148861

Following gene: 162148859

Centisome position: 80.76

GC content: 72.13

Gene sequence:

>714_bases
ATGACCTGGCGGGCCACCGTGCTGACCCTGTTCCCCGACATGTTCCCCGGCCCGCTGGGCCAGTCGCTGGCCGGCCGTGC
GCTGGAAGGCGGCCTGTGGTCGGTCGAGGCACGGAACCTGCGCGAGTACGGGCTGGGGCGGCATCGCGTCGTGGACGACA
CGCCGTTCGGGGGCGGGGCCGGCATGGTCATGCGTCCCGATGTGGTGTCGGCCGCGATCCGCGACGTGGCGCCGGCCGGG
CGTCCGCTGGTCTATCCCACGCCGCGCGGCCGCCCGCTGCGCCAGGACGACGTGCGCCGCTATGCCGGCGGCCCGGGCCT
GGTCGTGCTGTGCGGCCGGTACGAGGGGGTGGACGAACGGGCGCTGGAGGCCTGCGGTGCCGAACAGGTCTCGATCGGCG
ACTACGTCCTGTCGGGGGGGGAGATGGCGGCCCTGGTGCTGCTGGATTCCTGCGTCCGGCTGATCCCGGGGGTCATGGGC
TCCGACGCCAGCGGCACGGAGGAAAGCTTTTCCGACGGGCTGCTGGAATATCCGCACTACACCAAGCCGGCGAGCTGGGA
AGGGCGCGACGTGCCCGACATCCTGCTGTCCGGCCACCACGCCGCCATCGCCGGATGGCGGCAGACTGAATCCGAGACGG
CGACCCGGACCCGTCGGCCCGACCTGTGGGCCGCACATCTGGAGCGCACCGGCTGCACTGACAGACAGTTTTGA

Upstream 100 bases:

>100_bases
CCGCCGGCCGGATCGAGGCGGTCCTGCCGGACGAGATCGAGGTGCAGGGCGACCTGTCCGACAAGGGCGAGGTCGAGGTT
TCGGCAACCGGGGGTGCGTC

Downstream 100 bases:

>100_bases
AACGAGGGACTCGATCATGAACATTATCCAGCAGTATGAGGCCGACGAGATCGCGCGCCTTTCCGCCGCCCGCGAAGTGC
CGGAATTCGGCCCCGGCGAC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MTWRATVLTLFPDMFPGPLGQSLAGRALEGGLWSVEARNLREYGLGRHRVVDDTPFGGGAGMVMRPDVVSAAIRDVAPAG
RPLVYPTPRGRPLRQDDVRRYAGGPGLVVLCGRYEGVDERALEACGAEQVSIGDYVLSGGEMAALVLLDSCVRLIPGVMG
SDASGTEESFSDGLLEYPHYTKPASWEGRDVPDILLSGHHAAIAGWRQTESETATRTRRPDLWAAHLERTGCTDRQF

Sequences:

>Translated_237_residues
MTWRATVLTLFPDMFPGPLGQSLAGRALEGGLWSVEARNLREYGLGRHRVVDDTPFGGGAGMVMRPDVVSAAIRDVAPAG
RPLVYPTPRGRPLRQDDVRRYAGGPGLVVLCGRYEGVDERALEACGAEQVSIGDYVLSGGEMAALVLLDSCVRLIPGVMG
SDASGTEESFSDGLLEYPHYTKPASWEGRDVPDILLSGHHAAIAGWRQTESETATRTRRPDLWAAHLERTGCTDRQF
>Mature_236_residues
TWRATVLTLFPDMFPGPLGQSLAGRALEGGLWSVEARNLREYGLGRHRVVDDTPFGGGAGMVMRPDVVSAAIRDVAPAGR
PLVYPTPRGRPLRQDDVRRYAGGPGLVVLCGRYEGVDERALEACGAEQVSIGDYVLSGGEMAALVLLDSCVRLIPGVMGS
DASGTEESFSDGLLEYPHYTKPASWEGRDVPDILLSGHHAAIAGWRQTESETATRTRRPDLWAAHLERTGCTDRQF

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=237, Percent_Identity=44.3037974683544, Blast_Score=193, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_GLUDA (A9HS61)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001603321.1
- RefSeq:   YP_002277620.1
- ProteinModelPortal:   A9HS61
- SMR:   A9HS61
- GeneID:   5791918
- GeneID:   6976717
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_3278
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 25634; Mature: 25503

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWRATVLTLFPDMFPGPLGQSLAGRALEGGLWSVEARNLREYGLGRHRVVDDTPFGGGA
CCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECHHHHHHHCCCCCEECCCCCCCCCC
GMVMRPDVVSAAIRDVAPAGRPLVYPTPRGRPLRQDDVRRYAGGPGLVVLCGRYEGVDER
CCEECCHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHH
ALEACGAEQVSIGDYVLSGGEMAALVLLDSCVRLIPGVMGSDASGTEESFSDGLLEYPHY
HHHHCCCCCEECCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCC
TKPASWEGRDVPDILLSGHHAAIAGWRQTESETATRTRRPDLWAAHLERTGCTDRQF
CCCCCCCCCCCHHHHHCCCHHEEECCHHCCHHHHHHCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TWRATVLTLFPDMFPGPLGQSLAGRALEGGLWSVEARNLREYGLGRHRVVDDTPFGGGA
CCHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECHHHHHHHCCCCCEECCCCCCCCCC
GMVMRPDVVSAAIRDVAPAGRPLVYPTPRGRPLRQDDVRRYAGGPGLVVLCGRYEGVDER
CCEECCHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHH
ALEACGAEQVSIGDYVLSGGEMAALVLLDSCVRLIPGVMGSDASGTEESFSDGLLEYPHY
HHHHCCCCCEECCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCC
TKPASWEGRDVPDILLSGHHAAIAGWRQTESETATRTRRPDLWAAHLERTGCTDRQF
CCCCCCCCCCCHHHHHCCCHHEEECCHHCCHHHHHHCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA