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The map label for this gene is tig

Identifier: 162148816

GI number: 162148816

Start: 3137645

End: 3138982

Strand: Reverse

Name: tig

Synonym: GDI_3045

Alternate gene names: 162148816

Gene position: 3138982-3137645 (Counterclockwise)

Preceding gene: 162148817

Following gene: 162148815

Centisome position: 79.59

GC content: 66.97

Gene sequence:

>1338_bases
ATGCAGGTTACTGAAACGCTTTCCGCCGGCCTGAAGCGCGGATTTACCGTCACGGTGCCCGCCGGCGAACTGGAGAGCAA
GCGGACCGCCCGCCTGAAGGAACTGGGGCAGTCCATGAACCTGCCGGGCTTCCGTCCCGGCAAGGTGCCCCTGAGCATCG
TCAAGCAGCGCTACGGCGACGCCGTGCAGGGCGAGGTGCTGGAACAGGCGGTCAGCGACGCCACGCGCGCGCTGATGGAC
GAGCGTGGCCTGCGTCCGGCGATGCAGCCGCGCGTCGACCTGGTGGCGGGTGCCGAGCCGGGCGGCAAGGCCGACCTGGA
ATTCAAGGTCGAGGTCGAACTGCTGCCGGACATCGCGCAGCCCGACCTGTCCACCCTGTCGCTGACCCGCCTGAAGGCCA
CCCCGGACGCCGAGACGATCGACAAGGCGCTGAAGGACATCGCCAGCCGCCAGCGCGATTTCGAGACGATCGAGGACGTC
CGTCCGGCCGCGCAGGGCGACGTCGTGGTCGTGGATTTCGTCGGCAAGGTCGATGGCGTGGCGTTCGAGGGCGGCACCGC
CCAGGACGTCAATGTCGAACTCGGCGGCGCGGGCTTCATCCCCGGATTCGCCGAGCAGATCGAAGGCATGTCCCCGGGTG
AGGAGAAGGTCATCACCGTGACCTTCCCGGCGGATTACTCGGCCGAGAACCTGGCGGGCAAGGAAGCGACCTTCGACATC
ACCGCCAAGGCGCTGAAGCGCCCGGTCGATGTCGAGATCGACGACGAGATGGCGAAGAAGATCGGATTCGAGGGGCTGGA
ACAGGTCCGCGAACTGATCACCAGGCAGGTCGAGCAGGAATACGAGCAGCTGTCGCGCCTGCGCATCAAGCGCGAGCTGC
TGGACGCCCTGGCCGAGAAGACCGACTTCGAGGCCCCGCAGGGCATGGTCGAGGCCGAGTTCGCGCAGATCTGGCAGCGC
GTCGAAGCCGACCGGAAGGCCGGCGAGCTGGACGAGGAAGACAAGGAAAAGGACGAGGACACCCTGCGCGCCGACTACCG
CAAGATCGCGGAACGGCGGGTGAAGCTGGGCCTGCTGCTGGCCGAGATCGGCCGCGCCAACGCCATCACCGTCTCGCAGG
ACGAGATGCTGCAGGCCATCCGGGCCGAGGCCATGCGCTATCCCGGCCAGGAACAGCAGGTGTTCGAGTTCTTCCGCAAG
AACCCGCAGGCCGCCGAGAGCCTCCGTGGCCCGATCTTCGAGAACAAGGTTGTCGACTATGTGATCGAGCTGGCGACGGT
CGAGGACAAGGACGTCACCCCCGAGGAACTGGCCGAGATCCCGCCCGCCGACCTCTGA

Upstream 100 bases:

>100_bases
AGTCCCTCCGCCCGCACCAGCCCGGCCAGTCGCGCGCGGATGCCGGCCCGAAGGGCCCCGTGCGTTCTATGAAGTTCTGA
CCGAGAGGATGGCCTGGCAG

Downstream 100 bases:

>100_bases
GCGGCGCGGGTCCTGGATGGAACGCCCCGGCGCGCAGCGCGCGCCGGGCGCGGAGGACCGCCCTTCGCCGGATTTATCGC
CATGAAATGATGGCGGCGGG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MQVTETLSAGLKRGFTVTVPAGELESKRTARLKELGQSMNLPGFRPGKVPLSIVKQRYGDAVQGEVLEQAVSDATRALMD
ERGLRPAMQPRVDLVAGAEPGGKADLEFKVEVELLPDIAQPDLSTLSLTRLKATPDAETIDKALKDIASRQRDFETIEDV
RPAAQGDVVVVDFVGKVDGVAFEGGTAQDVNVELGGAGFIPGFAEQIEGMSPGEEKVITVTFPADYSAENLAGKEATFDI
TAKALKRPVDVEIDDEMAKKIGFEGLEQVRELITRQVEQEYEQLSRLRIKRELLDALAEKTDFEAPQGMVEAEFAQIWQR
VEADRKAGELDEEDKEKDEDTLRADYRKIAERRVKLGLLLAEIGRANAITVSQDEMLQAIRAEAMRYPGQEQQVFEFFRK
NPQAAESLRGPIFENKVVDYVIELATVEDKDVTPEELAEIPPADL

Sequences:

>Translated_445_residues
MQVTETLSAGLKRGFTVTVPAGELESKRTARLKELGQSMNLPGFRPGKVPLSIVKQRYGDAVQGEVLEQAVSDATRALMD
ERGLRPAMQPRVDLVAGAEPGGKADLEFKVEVELLPDIAQPDLSTLSLTRLKATPDAETIDKALKDIASRQRDFETIEDV
RPAAQGDVVVVDFVGKVDGVAFEGGTAQDVNVELGGAGFIPGFAEQIEGMSPGEEKVITVTFPADYSAENLAGKEATFDI
TAKALKRPVDVEIDDEMAKKIGFEGLEQVRELITRQVEQEYEQLSRLRIKRELLDALAEKTDFEAPQGMVEAEFAQIWQR
VEADRKAGELDEEDKEKDEDTLRADYRKIAERRVKLGLLLAEIGRANAITVSQDEMLQAIRAEAMRYPGQEQQVFEFFRK
NPQAAESLRGPIFENKVVDYVIELATVEDKDVTPEELAEIPPADL
>Mature_445_residues
MQVTETLSAGLKRGFTVTVPAGELESKRTARLKELGQSMNLPGFRPGKVPLSIVKQRYGDAVQGEVLEQAVSDATRALMD
ERGLRPAMQPRVDLVAGAEPGGKADLEFKVEVELLPDIAQPDLSTLSLTRLKATPDAETIDKALKDIASRQRDFETIEDV
RPAAQGDVVVVDFVGKVDGVAFEGGTAQDVNVELGGAGFIPGFAEQIEGMSPGEEKVITVTFPADYSAENLAGKEATFDI
TAKALKRPVDVEIDDEMAKKIGFEGLEQVRELITRQVEQEYEQLSRLRIKRELLDALAEKTDFEAPQGMVEAEFAQIWQR
VEADRKAGELDEEDKEKDEDTLRADYRKIAERRVKLGLLLAEIGRANAITVSQDEMLQAIRAEAMRYPGQEQQVFEFFRK
NPQAAESLRGPIFENKVVDYVIELATVEDKDVTPEELAEIPPADL

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=441, Percent_Identity=31.2925170068027, Blast_Score=181, Evalue=9e-47,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_GLUDA (A9HRV7)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001603277.1
- RefSeq:   YP_002277665.1
- ProteinModelPortal:   A9HRV7
- GeneID:   5791872
- GeneID:   6976764
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_3323
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49129; Mature: 49129

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVTETLSAGLKRGFTVTVPAGELESKRTARLKELGQSMNLPGFRPGKVPLSIVKQRYGD
CCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
AVQGEVLEQAVSDATRALMDERGLRPAMQPRVDLVAGAEPGGKADLEFKVEVELLPDIAQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCCCC
PDLSTLSLTRLKATPDAETIDKALKDIASRQRDFETIEDVRPAAQGDVVVVDFVGKVDGV
CCCHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCE
AFEGGTAQDVNVELGGAGFIPGFAEQIEGMSPGEEKVITVTFPADYSAENLAGKEATFDI
EECCCCCCCCEEEECCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCHHHCCCCCCHHHH
TAKALKRPVDVEIDDEMAKKIGFEGLEQVRELITRQVEQEYEQLSRLRIKRELLDALAEK
HHHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDFEAPQGMVEAEFAQIWQRVEADRKAGELDEEDKEKDEDTLRADYRKIAERRVKLGLLL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEIGRANAITVSQDEMLQAIRAEAMRYPGQEQQVFEFFRKNPQAAESLRGPIFENKVVDY
HHHCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHH
VIELATVEDKDVTPEELAEIPPADL
HHHHHHCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MQVTETLSAGLKRGFTVTVPAGELESKRTARLKELGQSMNLPGFRPGKVPLSIVKQRYGD
CCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
AVQGEVLEQAVSDATRALMDERGLRPAMQPRVDLVAGAEPGGKADLEFKVEVELLPDIAQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCCCC
PDLSTLSLTRLKATPDAETIDKALKDIASRQRDFETIEDVRPAAQGDVVVVDFVGKVDGV
CCCHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCE
AFEGGTAQDVNVELGGAGFIPGFAEQIEGMSPGEEKVITVTFPADYSAENLAGKEATFDI
EECCCCCCCCEEEECCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCHHHCCCCCCHHHH
TAKALKRPVDVEIDDEMAKKIGFEGLEQVRELITRQVEQEYEQLSRLRIKRELLDALAEK
HHHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDFEAPQGMVEAEFAQIWQRVEADRKAGELDEEDKEKDEDTLRADYRKIAERRVKLGLLL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEIGRANAITVSQDEMLQAIRAEAMRYPGQEQQVFEFFRKNPQAAESLRGPIFENKVVDY
HHHCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHH
VIELATVEDKDVTPEELAEIPPADL
HHHHHHCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA