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The map label for this gene is nouI [H]

Identifier: 162148803

GI number: 162148803

Start: 3123081

End: 3123569

Strand: Reverse

Name: nouI [H]

Synonym: GDI_3032

Alternate gene names: 162148803

Gene position: 3123569-3123081 (Counterclockwise)

Preceding gene: 162148804

Following gene: 162148802

Centisome position: 79.19

GC content: 62.78

Gene sequence:

>489_bases
ATGGGACATTTCGACCGGACGGTCCGTTCCTTCCTTCTGGCCGAGCTCGTGGCCGGAATGGGGGCCACGTTCAAGGCCAT
GTTCAAGCCCAAGGTGACGATCAACTATCCCTATGAAAAGGGACCGCTCTCGCCCCGCTTTCGTGGCGAGCACGCCCTGC
GCCGCTACCCGAACGGCGAGGAACGCTGCATCGCCTGCAAATTGTGCGAGGCGACGTGCCCGGCGGAAGCGATCACCATC
GAATCCGAACCCCGCGATGACGGGTCGCGGCGCACCACGCGCTACGACATCGACATGACGAAGTGCATCTATTGCGGCCT
GTGCGAGGAAGCATGCCCGGTCGATGCGATCGTCGAGGGACCGAATTACGAGTTCGCGACCGAGACGCGGGAGGAACTCA
TGTACAACAAGGACAAGCTGCTCGCCAACGGCGACCGCTGGGAAAGCGTCCTGGCACGGCGGCTGGAGCTCGATGCGCCC
TATCGTTGA

Upstream 100 bases:

>100_bases
TGGGATGGAAGGTGTTCCTTCCGTTCTCGCTGGTGTGGATGGTGCTGACGGCGGGCTTCCTGCTGGCGACCGGGCTTCTG
CCCCAGGGAGGAGCAAGCTG

Downstream 100 bases:

>100_bases
GACATCCGGCGTAGGGACATCCAGCGTGGGGACATCGAGCCCCGGGCATCGGGAGGACATATCCATGCGGCAGGCCCGCT
ACAAACAGCCCCGCTACAAA

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MGHFDRTVRSFLLAELVAGMGATFKAMFKPKVTINYPYEKGPLSPRFRGEHALRRYPNGEERCIACKLCEATCPAEAITI
ESEPRDDGSRRTTRYDIDMTKCIYCGLCEEACPVDAIVEGPNYEFATETREELMYNKDKLLANGDRWESVLARRLELDAP
YR

Sequences:

>Translated_162_residues
MGHFDRTVRSFLLAELVAGMGATFKAMFKPKVTINYPYEKGPLSPRFRGEHALRRYPNGEERCIACKLCEATCPAEAITI
ESEPRDDGSRRTTRYDIDMTKCIYCGLCEEACPVDAIVEGPNYEFATETREELMYNKDKLLANGDRWESVLARRLELDAP
YR
>Mature_161_residues
GHFDRTVRSFLLAELVAGMGATFKAMFKPKVTINYPYEKGPLSPRFRGEHALRRYPNGEERCIACKLCEATCPAEAITIE
SEPRDDGSRRTTRYDIDMTKCIYCGLCEEACPVDAIVEGPNYEFATETREELMYNKDKLLANGDRWESVLARRLELDAPY
R

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=158, Percent_Identity=72.7848101265823, Blast_Score=251, Evalue=3e-67,
Organism=Escherichia coli, GI1788617, Length=139, Percent_Identity=39.568345323741, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1789075, Length=126, Percent_Identity=29.3650793650794, Blast_Score=62, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555194, Length=161, Percent_Identity=68.944099378882, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI17864306, Length=158, Percent_Identity=72.1518987341772, Blast_Score=249, Evalue=8e-67,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18515; Mature: 18384

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHFDRTVRSFLLAELVAGMGATFKAMFKPKVTINYPYEKGPLSPRFRGEHALRRYPNGE
CCHHHHHHHHHHHHHHHHCCCCHHHEEECCEEEEECCCCCCCCCCCCCCCHHHHCCCCCC
ERCIACKLCEATCPAEAITIESEPRDDGSRRTTRYDIDMTKCIYCGLCEEACPVDAIVEG
CEEEEEEECCCCCCCCEEEECCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCCCCEEEEC
PNYEFATETREELMYNKDKLLANGDRWESVLARRLELDAPYR
CCCCCHHHHHHHHHHCCCHHEECCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GHFDRTVRSFLLAELVAGMGATFKAMFKPKVTINYPYEKGPLSPRFRGEHALRRYPNGE
CHHHHHHHHHHHHHHHHCCCCHHHEEECCEEEEECCCCCCCCCCCCCCCHHHHCCCCCC
ERCIACKLCEATCPAEAITIESEPRDDGSRRTTRYDIDMTKCIYCGLCEEACPVDAIVEG
CEEEEEEECCCCCCCCEEEECCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCCCCEEEEC
PNYEFATETREELMYNKDKLLANGDRWESVLARRLELDAPYR
CCCCCHHHHHHHHHHCCCHHEECCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA