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The map label for this gene is bioB [H]

Identifier: 162148725

GI number: 162148725

Start: 3041613

End: 3042713

Strand: Direct

Name: bioB [H]

Synonym: GDI_2953

Alternate gene names: 162148725

Gene position: 3041613-3042713 (Clockwise)

Preceding gene: 162148724

Following gene: 162148726

Centisome position: 77.12

GC content: 69.21

Gene sequence:

>1101_bases
TTGCGCGGGCCGGGCACGCCTTGGGTGACACCGCCCCCCCTTCTGTGGCAGAGCGTGTTCCTTATCGCCGACAGTCCAGG
AACCAGCCCGATGCCCGATGGTGTCATCCCGTCCACCTCGCCGTGTCATGCCGACCCGTCGCTTCCGCCGGTGCGCACCG
ACTGGACGCGGGACGAGATCGCGGCGCTGATCGCCCTGCCCTTCCCCGAACTGATGTTCCGGGCGCAGAGCGTGCATCGC
GCCCGCTTCGACCCCACCGAGATCCAGATTTCCACCCTGCTGTCGATCAAGACCGGCGGCTGCCCCGAGGATTGCGCCTA
CTGCCCGCAGAGCGCGAAGCACGAGGACGGCGGCGTGAAGGCCAGCCGCCTGATGGCGGTGGAGGCCGTGCTGAAGGAAG
CCCGGGCGGCGCGGGACGCCGGGGCCGCGCGCTTCTGCATGGGCGCCGCGTGGCGTTCGCCCAAGGATCATGACCTGGAG
ACCGTTTGCGCCATGGTCGAGGGCGTGAAGAGCCTGGGCATGGAGACCTGCGTCACGCTGGGCATGCTGGACGACCGGCA
GGCGCACCGCCTGCGCGAGGCCGGGCTGGATTACTACAACCACAATCTGGACACCTCGCCCGAGCATTACGGGTCCATCA
TTTCCACCCGCACCTATCAGGAGCGGCTGGATACGCTGGCGCATGTGCGCGATGCCGGGATCAATGTCTGCTGCGGCGGC
ATCGTGGGCATGGGCGAGAACGACGACGACCGCGCGGGGCTGATCGCCAGCCTGGCCAGCCTGCCGCGGCACCCCGAAAG
CGTGCCGATCAACCTGCTGGTCCGCGTGGCGGGAACGCCGCTGGCCGGGGCCGATCCGGTCGATCCGATCGACTTCGTCC
GCATCATCGCCGCCGCGCGCATCGCCATGCCGGCCAGCCGGGTACGGCTGGCGGCGGGGCGCGAGGACATGACGGACGAA
GCGCAGACCCTGTGCTTCCTGGCCGGCGCGAATTCGATCTTCTATGGCGAGAAGCTGCTGACCACCCCCAACCCCGAAGC
CAGCCGCGATGCCAGGCTGCTCGCGCGGCTGGGGATGCATACGAGTCTGGCCCAGGCCTGA

Upstream 100 bases:

>100_bases
ATCCATGTCTCGATCAGCCCGGCGCTGCTGGGCCATACGCTGCTGGAGGTCATGGCGGGCCGCGCCTGACCCGCCCGCCG
CACAAGGCGTGCCCGTCCCG

Downstream 100 bases:

>100_bases
ACGCCGTCATTTAGCACTTGCGTCGCGCAGCGGCGTGTCGGAAACCACCGCCATGTCGTATGCGATCAAGGAGATGTTCG
CGACCCTCCAGGGCGAGGGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MRGPGTPWVTPPPLLWQSVFLIADSPGTSPMPDGVIPSTSPCHADPSLPPVRTDWTRDEIAALIALPFPELMFRAQSVHR
ARFDPTEIQISTLLSIKTGGCPEDCAYCPQSAKHEDGGVKASRLMAVEAVLKEARAARDAGAARFCMGAAWRSPKDHDLE
TVCAMVEGVKSLGMETCVTLGMLDDRQAHRLREAGLDYYNHNLDTSPEHYGSIISTRTYQERLDTLAHVRDAGINVCCGG
IVGMGENDDDRAGLIASLASLPRHPESVPINLLVRVAGTPLAGADPVDPIDFVRIIAAARIAMPASRVRLAAGREDMTDE
AQTLCFLAGANSIFYGEKLLTTPNPEASRDARLLARLGMHTSLAQA

Sequences:

>Translated_366_residues
MRGPGTPWVTPPPLLWQSVFLIADSPGTSPMPDGVIPSTSPCHADPSLPPVRTDWTRDEIAALIALPFPELMFRAQSVHR
ARFDPTEIQISTLLSIKTGGCPEDCAYCPQSAKHEDGGVKASRLMAVEAVLKEARAARDAGAARFCMGAAWRSPKDHDLE
TVCAMVEGVKSLGMETCVTLGMLDDRQAHRLREAGLDYYNHNLDTSPEHYGSIISTRTYQERLDTLAHVRDAGINVCCGG
IVGMGENDDDRAGLIASLASLPRHPESVPINLLVRVAGTPLAGADPVDPIDFVRIIAAARIAMPASRVRLAAGREDMTDE
AQTLCFLAGANSIFYGEKLLTTPNPEASRDARLLARLGMHTSLAQA
>Mature_366_residues
MRGPGTPWVTPPPLLWQSVFLIADSPGTSPMPDGVIPSTSPCHADPSLPPVRTDWTRDEIAALIALPFPELMFRAQSVHR
ARFDPTEIQISTLLSIKTGGCPEDCAYCPQSAKHEDGGVKASRLMAVEAVLKEARAARDAGAARFCMGAAWRSPKDHDLE
TVCAMVEGVKSLGMETCVTLGMLDDRQAHRLREAGLDYYNHNLDTSPEHYGSIISTRTYQERLDTLAHVRDAGINVCCGG
IVGMGENDDDRAGLIASLASLPRHPESVPINLLVRVAGTPLAGADPVDPIDFVRIIAAARIAMPASRVRLAAGREDMTDE
AQTLCFLAGANSIFYGEKLLTTPNPEASRDARLLARLGMHTSLAQA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=309, Percent_Identity=61.8122977346278, Blast_Score=413, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=47.1337579617834, Blast_Score=309, Evalue=5e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 39369; Mature: 39369

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGPGTPWVTPPPLLWQSVFLIADSPGTSPMPDGVIPSTSPCHADPSLPPVRTDWTRDEI
CCCCCCCCCCCCHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
AALIALPFPELMFRAQSVHRARFDPTEIQISTLLSIKTGGCPEDCAYCPQSAKHEDGGVK
HHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHCCCCCCCCCCCCH
ASRLMAVEAVLKEARAARDAGAARFCMGAAWRSPKDHDLETVCAMVEGVKSLGMETCVTL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHH
GMLDDRQAHRLREAGLDYYNHNLDTSPEHYGSIISTRTYQERLDTLAHVRDAGINVCCGG
CCCCCHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
IVGMGENDDDRAGLIASLASLPRHPESVPINLLVRVAGTPLAGADPVDPIDFVRIIAAAR
EEECCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
IAMPASRVRLAAGREDMTDEAQTLCFLAGANSIFYGEKLLTTPNPEASRDARLLARLGMH
HHCCHHHHHHHCCCCCCHHHHHHEEHEECCCCEEECCEEECCCCCCCHHHHHHHHHHCCH
TSLAQA
HHHHCC
>Mature Secondary Structure
MRGPGTPWVTPPPLLWQSVFLIADSPGTSPMPDGVIPSTSPCHADPSLPPVRTDWTRDEI
CCCCCCCCCCCCHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
AALIALPFPELMFRAQSVHRARFDPTEIQISTLLSIKTGGCPEDCAYCPQSAKHEDGGVK
HHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHCCCCCCCCCCCCH
ASRLMAVEAVLKEARAARDAGAARFCMGAAWRSPKDHDLETVCAMVEGVKSLGMETCVTL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHH
GMLDDRQAHRLREAGLDYYNHNLDTSPEHYGSIISTRTYQERLDTLAHVRDAGINVCCGG
CCCCCHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
IVGMGENDDDRAGLIASLASLPRHPESVPINLLVRVAGTPLAGADPVDPIDFVRIIAAAR
EEECCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
IAMPASRVRLAAGREDMTDEAQTLCFLAGANSIFYGEKLLTTPNPEASRDARLLARLGMH
HHCCHHHHHHHCCCCCCHHHHHHEEHEECCCCEEECCEEECCCCCCCHHHHHHHHHHCCH
TSLAQA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA