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The map label for this gene is raiI [H]

Identifier: 162148609

GI number: 162148609

Start: 2928234

End: 2928863

Strand: Reverse

Name: raiI [H]

Synonym: GDI_2836

Alternate gene names: 162148609

Gene position: 2928863-2928234 (Counterclockwise)

Preceding gene: 162148614

Following gene: 162148605

Centisome position: 74.26

GC content: 49.21

Gene sequence:

>630_bases
ATGCAGACCTATGTATTTTCCTATTCTAGCCGCTCGCAAGTTCCAGATATATATTTGCAAATGAGCAAGGCGAGATGTCG
GGTGTTTAAGGAGCGTCTGGGCTGGGATGTGCACGACGACGATGGATTGGAAGTCGACGAGTTCGATACGACGTTGGACC
CTCTTTATTTCGTCGTCGCCGACAACCGGGGCAAACATATTGCCTCCCTGAGATTGCTTTCCTGCAAAGGACCAACAATG
CTAACCCGAATATTCCAAGATTTTTTTCCCGCTGGCTCTGTGCGGGTTTGTAATGCCGCCTTTGAATGTACGCGACTCTG
CCTTTATGCTCCCGGGATCGATGAACCGCTTCGTCGGATCGGAATTGCGCAGTTGTGCTACACGCTAAGCAATTATGCAC
TTTCGCAGAATGCGTCGGGCATTATAGGTGTGTATTATTCGATCATGCGGAGAGTGTACGCAAAGGTTGGGTGGGCACCC
GACACTCTGGTTAGTTCTATTCAAGAAGATAAAAATATTGAGCTGGGTCGATGGAATGTGTCGGAGGAGGTGGTAGGCTC
GCTTGGCGCTCTATGTTGGCGCCTGTCGCTTGATATACCCCATTATAATCGCGTCATTAAGGTCGAATAA

Upstream 100 bases:

>100_bases
ATGACTTCCGCTATATCGATATGGAGATACTAGGGCTGGTGAATGGATAGCATATCTTCCGTCACGTAGATCGGTTTCCG
TTAAATCGGAGACATCATCG

Downstream 100 bases:

>100_bases
TACCCGTGCACCGGGTCGCGGGGCTGGCAGGGTGATCATTCTGAAGCTGTCGCAACTATTCCGGCTGAGGACGCCATGGT
TTCGTGCTTTCCGCTCAAAG

Product: autoinducer synthesis protein

Products: NA

Alternate protein names: Autoinducer synthesis protein raiI [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MQTYVFSYSSRSQVPDIYLQMSKARCRVFKERLGWDVHDDDGLEVDEFDTTLDPLYFVVADNRGKHIASLRLLSCKGPTM
LTRIFQDFFPAGSVRVCNAAFECTRLCLYAPGIDEPLRRIGIAQLCYTLSNYALSQNASGIIGVYYSIMRRVYAKVGWAP
DTLVSSIQEDKNIELGRWNVSEEVVGSLGALCWRLSLDIPHYNRVIKVE

Sequences:

>Translated_209_residues
MQTYVFSYSSRSQVPDIYLQMSKARCRVFKERLGWDVHDDDGLEVDEFDTTLDPLYFVVADNRGKHIASLRLLSCKGPTM
LTRIFQDFFPAGSVRVCNAAFECTRLCLYAPGIDEPLRRIGIAQLCYTLSNYALSQNASGIIGVYYSIMRRVYAKVGWAP
DTLVSSIQEDKNIELGRWNVSEEVVGSLGALCWRLSLDIPHYNRVIKVE
>Mature_209_residues
MQTYVFSYSSRSQVPDIYLQMSKARCRVFKERLGWDVHDDDGLEVDEFDTTLDPLYFVVADNRGKHIASLRLLSCKGPTM
LTRIFQDFFPAGSVRVCNAAFECTRLCLYAPGIDEPLRRIGIAQLCYTLSNYALSQNASGIIGVYYSIMRRVYAKVGWAP
DTLVSSIQEDKNIELGRWNVSEEVVGSLGALCWRLSLDIPHYNRVIKVE

Specific function: Required for the synthesis of autoinducer molecules which bind to raiR and that are involved in the restriction of nodule number [H]

COG id: COG3916

COG function: function code TQ; N-acyl-L-homoserine lactone synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the autoinducer synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR001690
- InterPro:   IPR018311 [H]

Pfam domain/function: PF00765 Autoind_synth [H]

EC number: =2.3.1.184 [H]

Molecular weight: Translated: 23751; Mature: 23751

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00949 AUTOINDUCERS_SYNTH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTYVFSYSSRSQVPDIYLQMSKARCRVFKERLGWDVHDDDGLEVDEFDTTLDPLYFVVA
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCEEEEEE
DNRGKHIASLRLLSCKGPTMLTRIFQDFFPAGSVRVCNAAFECTRLCLYAPGIDEPLRRI
CCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHEECCCCCHHHHHH
GIAQLCYTLSNYALSQNASGIIGVYYSIMRRVYAKVGWAPDTLVSSIQEDKNIELGRWNV
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCC
SEEVVGSLGALCWRLSLDIPHYNRVIKVE
HHHHHHHHHHHHHHHCCCCCCCCCEEEEC
>Mature Secondary Structure
MQTYVFSYSSRSQVPDIYLQMSKARCRVFKERLGWDVHDDDGLEVDEFDTTLDPLYFVVA
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCEEEEEE
DNRGKHIASLRLLSCKGPTMLTRIFQDFFPAGSVRVCNAAFECTRLCLYAPGIDEPLRRI
CCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHEECCCCCHHHHHH
GIAQLCYTLSNYALSQNASGIIGVYYSIMRRVYAKVGWAPDTLVSSIQEDKNIELGRWNV
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCC
SEEVVGSLGALCWRLSLDIPHYNRVIKVE
HHHHHHHHHHHHHHHCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9473034 [H]