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The map label for this gene is entA [C]

Identifier: 162148560

GI number: 162148560

Start: 2870610

End: 2871590

Strand: Direct

Name: entA [C]

Synonym: GDI_2784

Alternate gene names: 162148560

Gene position: 2870610-2871590 (Clockwise)

Preceding gene: 162148558

Following gene: 162148561

Centisome position: 72.78

GC content: 63.2

Gene sequence:

>981_bases
ATGACTCAGATCTTTGACGCGACCTCGACGACCGACGATGTTCTCTCCGGCATATCCCTGAAAGGCAAGCGCGTTCTCGT
GACCGGCGTTTCCGCCGGACTGGGCATTGAAACGGCCCGGGCACTGGCAGGTCATGGCGCGCATATCGTGGGCGCGGCGC
GCGATCTTGCGAAAGCGGAACGCGCAACCGATCAGGTTCGCGTGGCCGCGTCGCAAGGAGGCGGAGCGTTCGAACTCACC
GCGCTCGACCTTGCGGATCTGGCAAGTGTGCGTGCCTGCGCCGACCGTCTGAATGCGCAAGGCACGCCCTTCGACCTGGT
CATCGCCAACGCGGGCGTGATGGCGACACCATTCGGGCATACGAAGGACGGGTTCGAGACGCAGTTTGGCACCAACCATC
TGGGACATTTCGTTCTGGTCAACCGAATTGCCGGACTACTGCGCGACGGCGCACGACTGGTCAATGTGTCCTCGGCCGGA
CATCGCTTCGCCGATGTCGATCTCGACGATCCGAATTTCGAGCACACGCCGTACGTGCCGTTCGTGGCCTATGGACGTTC
CAAGACCGCCAATATTCTCTTCGCCGTTGCCTTCGATGCGCGGCATCGCGCACGGGGCGTGCGGGCGACGGCGGTTCACC
CCGGTGGGATTGTGACGGAACTGGCGCGGCACATGGCACCCGGGGAGATCGAAGCCATGTTGGACCAGATCAACAAGCAG
GCCACCGCCGAGGGCCAGAAGCCGTTCCAGTTCAAGAGCATTCCGCAGGGGGCTGCAACCTCGGTCTGGGCCGGCGTCGT
GGCTGAAGCCGGTGCGGTAGGCGCTCATTACTGCGAGGATTGCCACGTCAGTGATGTCGTGCCGAACGACCAGCCGATCA
GTCTGGTCAACGCGGGCGTACGCGCCTACGCTCTTGATCCGGCACACGCCGAAGCCCTGTGGACAAAAAGCGAGGAGATG
GTCGGCGAACGCTTCGCATGA

Upstream 100 bases:

>100_bases
AGTTATCCTCCGTATATATCAAACGGAGCGATGCTCCACATAGAGCCAATGTTGCCATATCGGCAAGAAGTGCCCGCACC
GTTCCAAGGGAGTTCTACCC

Downstream 100 bases:

>100_bases
CACGATCGCGCGAACAGCGCTTCGATACAGGGCAAGCTCGCAGATTGGCCCAGGGCGCCGACGGCCAACACACGCCGTCG
GGCCCACCTATCCATGAGGA

Product: dehydrogenase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTQIFDATSTTDDVLSGISLKGKRVLVTGVSAGLGIETARALAGHGAHIVGAARDLAKAERATDQVRVAASQGGGAFELT
ALDLADLASVRACADRLNAQGTPFDLVIANAGVMATPFGHTKDGFETQFGTNHLGHFVLVNRIAGLLRDGARLVNVSSAG
HRFADVDLDDPNFEHTPYVPFVAYGRSKTANILFAVAFDARHRARGVRATAVHPGGIVTELARHMAPGEIEAMLDQINKQ
ATAEGQKPFQFKSIPQGAATSVWAGVVAEAGAVGAHYCEDCHVSDVVPNDQPISLVNAGVRAYALDPAHAEALWTKSEEM
VGERFA

Sequences:

>Translated_326_residues
MTQIFDATSTTDDVLSGISLKGKRVLVTGVSAGLGIETARALAGHGAHIVGAARDLAKAERATDQVRVAASQGGGAFELT
ALDLADLASVRACADRLNAQGTPFDLVIANAGVMATPFGHTKDGFETQFGTNHLGHFVLVNRIAGLLRDGARLVNVSSAG
HRFADVDLDDPNFEHTPYVPFVAYGRSKTANILFAVAFDARHRARGVRATAVHPGGIVTELARHMAPGEIEAMLDQINKQ
ATAEGQKPFQFKSIPQGAATSVWAGVVAEAGAVGAHYCEDCHVSDVVPNDQPISLVNAGVRAYALDPAHAEALWTKSEEM
VGERFA
>Mature_325_residues
TQIFDATSTTDDVLSGISLKGKRVLVTGVSAGLGIETARALAGHGAHIVGAARDLAKAERATDQVRVAASQGGGAFELTA
LDLADLASVRACADRLNAQGTPFDLVIANAGVMATPFGHTKDGFETQFGTNHLGHFVLVNRIAGLLRDGARLVNVSSAGH
RFADVDLDDPNFEHTPYVPFVAYGRSKTANILFAVAFDARHRARGVRATAVHPGGIVTELARHMAPGEIEAMLDQINKQA
TAEGQKPFQFKSIPQGAATSVWAGVVAEAGAVGAHYCEDCHVSDVVPNDQPISLVNAGVRAYALDPAHAEALWTKSEEMV
GERFA

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7706523, Length=333, Percent_Identity=34.5345345345345, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI10190746, Length=317, Percent_Identity=35.3312302839117, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI186928839, Length=312, Percent_Identity=34.6153846153846, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI166795268, Length=309, Percent_Identity=33.6569579288026, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI225579078, Length=211, Percent_Identity=40.2843601895735, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI193804850, Length=308, Percent_Identity=31.1688311688312, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI19923931, Length=145, Percent_Identity=43.448275862069, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI146231950, Length=311, Percent_Identity=29.903536977492, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193207620, Length=339, Percent_Identity=31.5634218289086, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17532805, Length=328, Percent_Identity=31.4024390243902, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71992402, Length=329, Percent_Identity=28.8753799392097, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17532791, Length=288, Percent_Identity=29.8611111111111, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17535059, Length=325, Percent_Identity=28.6153846153846, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17558006, Length=322, Percent_Identity=30.7453416149068, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32565532, Length=230, Percent_Identity=31.304347826087, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17505416, Length=262, Percent_Identity=30.5343511450382, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI72001364, Length=233, Percent_Identity=27.0386266094421, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324820, Length=221, Percent_Identity=28.5067873303167, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129347, Length=327, Percent_Identity=31.8042813455657, Blast_Score=160, Evalue=8e-40,
Organism=Drosophila melanogaster, GI45554248, Length=304, Percent_Identity=35.5263157894737, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24640117, Length=304, Percent_Identity=35.5263157894737, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI22024069, Length=307, Percent_Identity=34.2019543973941, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI19921754, Length=304, Percent_Identity=33.5526315789474, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI28573296, Length=306, Percent_Identity=32.3529411764706, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24586328, Length=303, Percent_Identity=32.3432343234323, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI161076371, Length=262, Percent_Identity=34.3511450381679, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24647946, Length=310, Percent_Identity=30.9677419354839, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45553409, Length=309, Percent_Identity=31.0679611650485, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24647948, Length=309, Percent_Identity=31.0679611650485, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24656154, Length=264, Percent_Identity=30.3030303030303, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922628, Length=264, Percent_Identity=30.3030303030303, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24647931, Length=295, Percent_Identity=26.7796610169492, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 34242; Mature: 34111

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQIFDATSTTDDVLSGISLKGKRVLVTGVSAGLGIETARALAGHGAHIVGAARDLAKAE
CCEECCCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEHHHHHHHHHH
RATDQVRVAASQGGGAFELTALDLADLASVRACADRLNAQGTPFDLVIANAGVMATPFGH
HHHHHEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCC
TKDGFETQFGTNHLGHFVLVNRIAGLLRDGARLVNVSSAGHRFADVDLDDPNFEHTPYVP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCCCCCCCC
FVAYGRSKTANILFAVAFDARHRARGVRATAVHPGGIVTELARHMAPGEIEAMLDQINKQ
EEEECCCCCCEEEEEEEECCHHHHCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHH
ATAEGQKPFQFKSIPQGAATSVWAGVVAEAGAVGAHYCEDCHVSDVVPNDQPISLVNAGV
HHCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCHHHHHCCC
RAYALDPAHAEALWTKSEEMVGERFA
EEEEECCHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
TQIFDATSTTDDVLSGISLKGKRVLVTGVSAGLGIETARALAGHGAHIVGAARDLAKAE
CEECCCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEHHHHHHHHHH
RATDQVRVAASQGGGAFELTALDLADLASVRACADRLNAQGTPFDLVIANAGVMATPFGH
HHHHHEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCC
TKDGFETQFGTNHLGHFVLVNRIAGLLRDGARLVNVSSAGHRFADVDLDDPNFEHTPYVP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCCCCCCCC
FVAYGRSKTANILFAVAFDARHRARGVRATAVHPGGIVTELARHMAPGEIEAMLDQINKQ
EEEECCCCCCEEEEEEEECCHHHHCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHH
ATAEGQKPFQFKSIPQGAATSVWAGVVAEAGAVGAHYCEDCHVSDVVPNDQPISLVNAGV
HHCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCHHHHHCCC
RAYALDPAHAEALWTKSEEMVGERFA
EEEEECCHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]